PHS 398 (Rev. 9/04), Biographical Sketch Format Page
... identifying new interacting proteins and post-translational modifications will require technical and computational expertise, and biological insight. My graduate and post-graduate work in each of these areas helped advance the nascent proteomics field to its current state: pioneering investigations ...
... identifying new interacting proteins and post-translational modifications will require technical and computational expertise, and biological insight. My graduate and post-graduate work in each of these areas helped advance the nascent proteomics field to its current state: pioneering investigations ...
Where in the cell is your protein most likely found?
... • All proteins are synthesized in the cytoplasm. • Proteins with export signals can be directed to other cellular locations: – cytoplasm, cytoplasmic membrane, outer membrane or periplasm of Gram (-) bacteria, cell wall, or as secreted products in extracellular space ...
... • All proteins are synthesized in the cytoplasm. • Proteins with export signals can be directed to other cellular locations: – cytoplasm, cytoplasmic membrane, outer membrane or periplasm of Gram (-) bacteria, cell wall, or as secreted products in extracellular space ...
shRNA Customer Presentation
... E. Coli was transformed with 4 constructs with mammalian ORFs in the pEX-N-His-GST vector. GST-fused proteins were expressed at high levels with all constructs. ...
... E. Coli was transformed with 4 constructs with mammalian ORFs in the pEX-N-His-GST vector. GST-fused proteins were expressed at high levels with all constructs. ...
Bacteria with vuvuzelas: Microbes use a channel protein
... structure. "It may be that a drop in electrical tension or some specific unpacking proteins such as TcB are necessary for TcC to exit the vesicle and enter the cell, where it can unleash its deadly effects." The results show that the TcA of roundworm bacteria is similar in shape to the toxins of the ...
... structure. "It may be that a drop in electrical tension or some specific unpacking proteins such as TcB are necessary for TcC to exit the vesicle and enter the cell, where it can unleash its deadly effects." The results show that the TcA of roundworm bacteria is similar in shape to the toxins of the ...
Ty Samo Seminar (PDF)
... Activity Measurements in Marine Bacteria" Continuing advances in fluorescence tagging, staining, and microscopy have enabled the efficient and sensitive measurement of cell-specific metabolism. Traditionally developed for and applied to biomedical model systems, these tools are well suited for micro ...
... Activity Measurements in Marine Bacteria" Continuing advances in fluorescence tagging, staining, and microscopy have enabled the efficient and sensitive measurement of cell-specific metabolism. Traditionally developed for and applied to biomedical model systems, these tools are well suited for micro ...
Eating for Lean Body Mass By Kelly Drew, Registered Dietitian
... While it’s true that calories burned must exceed calories eaten in order to achieve weight loss, the types of calories we consume are important. Loss of lean body mass (muscle) in addition to loss of fat mass can occur when eating unbalanced meals or insufficient protein while following a reducedcal ...
... While it’s true that calories burned must exceed calories eaten in order to achieve weight loss, the types of calories we consume are important. Loss of lean body mass (muscle) in addition to loss of fat mass can occur when eating unbalanced meals or insufficient protein while following a reducedcal ...
A new strategy for quantitative proteomics using isotope
... Quantitative proteome analyses usually are accomplished by 2Delectrophoresis (2DE) followed by mass spectrometric protein identification. Although this method is well established, quantitative determination is not accurate and the reproducibility of the 2D-gels is very poor. Recent developments, lik ...
... Quantitative proteome analyses usually are accomplished by 2Delectrophoresis (2DE) followed by mass spectrometric protein identification. Although this method is well established, quantitative determination is not accurate and the reproducibility of the 2D-gels is very poor. Recent developments, lik ...
Sample preparation and analytical strategies for
... and dephosphorylated within minutes, and protease and phosphatase inhibitors must be used during cell lysis and protein extraction to preserve the integrity of proteins and their phosphorylation status [45]. Cocktails of phosphatase inhibitors generally contain sodium orthovanadate (tyrosine phospha ...
... and dephosphorylated within minutes, and protease and phosphatase inhibitors must be used during cell lysis and protein extraction to preserve the integrity of proteins and their phosphorylation status [45]. Cocktails of phosphatase inhibitors generally contain sodium orthovanadate (tyrosine phospha ...
Laura Bassi Centres of Expertise - PlantBioP Plant
... delivery of the respective DNA constructs into plants (magnicon®: Marillonnet et al., 2005); (ii) the generation of glycoengineered plant mutants that allow incorporation of a homogeneous, human-like glycosylation pattern into the recombinant proteins (Strasser et al., 2008, Strasser et al., 2009). ...
... delivery of the respective DNA constructs into plants (magnicon®: Marillonnet et al., 2005); (ii) the generation of glycoengineered plant mutants that allow incorporation of a homogeneous, human-like glycosylation pattern into the recombinant proteins (Strasser et al., 2008, Strasser et al., 2009). ...
The Genetic Evolution of H5N1
... H5N1 avian influenza was initially confined to poultry, but in recent years it has emerged as a highly fatal infectious disease in the human population. In 1997 in Hong Kong, H5N1 crossed the avian-human species barrier for the first time. Highly pathogenic H5N1 influenza A viruses have spread relen ...
... H5N1 avian influenza was initially confined to poultry, but in recent years it has emerged as a highly fatal infectious disease in the human population. In 1997 in Hong Kong, H5N1 crossed the avian-human species barrier for the first time. Highly pathogenic H5N1 influenza A viruses have spread relen ...
Protein kinases
... • Like falling dominoes, the receptor activates another protein, which activates another, and so on, until the protein producing the response is activated • At each step, the signal is transduced into a different form, usually a shape change in a protein ...
... • Like falling dominoes, the receptor activates another protein, which activates another, and so on, until the protein producing the response is activated • At each step, the signal is transduced into a different form, usually a shape change in a protein ...
Animal Nutrition
... • main source of energy • include sugars, starches and cellulose • almost all come from plantswheat, oats, barley ...
... • main source of energy • include sugars, starches and cellulose • almost all come from plantswheat, oats, barley ...
Chapter 3 Presentation: Macromolecules
... in plants (starch), and that which is found in animals (glycogen). • Glycogen is branched, and starch is helical. ...
... in plants (starch), and that which is found in animals (glycogen). • Glycogen is branched, and starch is helical. ...
Dr. Elisar Barbar`s Lab - Oregon State University
... • Two classes of dynein – Axonemal dynein • propels beating of cilia and flagella – Cytoplasmic dynein • transports membrane bound vesicles, protein complexes, chromosomes • Cellular organization • Cell Division ...
... • Two classes of dynein – Axonemal dynein • propels beating of cilia and flagella – Cytoplasmic dynein • transports membrane bound vesicles, protein complexes, chromosomes • Cellular organization • Cell Division ...
DNA/Protein structure-function analysis and prediction - IBIVU
... secondary structural domains (represented by a circle and square) linked by polypeptide linkers (hinge loops). The interface between domains in the closed monomer is referred to as the C- (closed) interface. Closed monomers may be opened by mildly denaturing conditions or by mutations that destabili ...
... secondary structural domains (represented by a circle and square) linked by polypeptide linkers (hinge loops). The interface between domains in the closed monomer is referred to as the C- (closed) interface. Closed monomers may be opened by mildly denaturing conditions or by mutations that destabili ...
4. Appraising the Proximate Analysis System
... Oven-drying only measures physically bound water – Even at 130O C leaves some chemically bound water (water of hydration, chemically bound to minerals) Drying above 60o C can create artifacts that hinder lignin, fiber & ADF analysis ...
... Oven-drying only measures physically bound water – Even at 130O C leaves some chemically bound water (water of hydration, chemically bound to minerals) Drying above 60o C can create artifacts that hinder lignin, fiber & ADF analysis ...
Protein phosphorylation in chloroplasts – a survey of
... at putative tyrosine phosphorylated chloroplast proteins (Lu et al., 2015b). In a large-scale meta-analysis based on data available in PhosphAT, 27 published papers as well as unpublished in-house data from the Schulze laboratory were integrated into one dataset. With this combination, a surprising ...
... at putative tyrosine phosphorylated chloroplast proteins (Lu et al., 2015b). In a large-scale meta-analysis based on data available in PhosphAT, 27 published papers as well as unpublished in-house data from the Schulze laboratory were integrated into one dataset. With this combination, a surprising ...
Mucolipin 1 channel activity is regulated by protein kinase A
... Phosphorylation is another modification that critically regulates protein activity. The activity of many TRP channels is regulated by phosphorylation and dephosphorylation events. A comprehensive overview of the regulation of TRP channels, including phosphorylation and interaction with auxiliary pro ...
... Phosphorylation is another modification that critically regulates protein activity. The activity of many TRP channels is regulated by phosphorylation and dephosphorylation events. A comprehensive overview of the regulation of TRP channels, including phosphorylation and interaction with auxiliary pro ...
MCB Seminar 4B, 2015 : Translation
... 1. There are much less eIF2B molecules than eIF2 molecules in the eukaryotic cell. In a particular cell line there is 1 eIF2B per 10 eIF2. What would you expect to happen with the protein synthesis if the level of eIF2 phosphorylation by PKR is (a) 5% ; (b) 20% ? Explain why. ...
... 1. There are much less eIF2B molecules than eIF2 molecules in the eukaryotic cell. In a particular cell line there is 1 eIF2B per 10 eIF2. What would you expect to happen with the protein synthesis if the level of eIF2 phosphorylation by PKR is (a) 5% ; (b) 20% ? Explain why. ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.