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Analysis of the 3′-terminal nucleotide sequence of vesicular
Analysis of the 3′-terminal nucleotide sequence of vesicular

... transcription, 10 x the concentration of the corresponding dNTP; (b) for 10 - 100 nucleotides, 1 x the dNTP concentration; (c) for more than 100 nucleotides, 0.5 x or 0,25 x. the dNTP concentration. The products of the reactions were fractionated by electrophoresis in polyacrylamide slab gels contai ...
Localization of the P1 protein of potato Y potyvirus in association
Localization of the P1 protein of potato Y potyvirus in association

... Osman, T. A. M., Tho$ mmes, P. & Buck, K. W. (1993). Localization of a single-stranded RNA-binding domain in the movement protein of red clover necrotic mosaic dianthovirus. Journal of General Virology 74, ...
Biochemistry
Biochemistry

... b) enzyme may change shape to help substrate ...
Protein 1 File
Protein 1 File

... elements of separate polymer chains • may be promoted by chaperones, membrane proteins, cytosolic and extracellular elements as well as the proteins’ own propensities • E decreases further due to further desolvation and reduction of surface area • globular proteins, e.g. hemoglobin, largely involve ...
Download PDF
Download PDF

... that occur in living organisms. In order to truly understand the detailed mechanisms of these diverse reactions, one must assimilate aspects of organic chemistry, inorganic chemistry, and physical chemistry and apply these chemical principles to the complex structural environment presented by natura ...
91159 Demonstrate understanding of gene expression
91159 Demonstrate understanding of gene expression

... Biological ideas and processes relating to the determination of phenotype via metabolic pathways are selected from:  biochemical reactions are catalysed by specific enzymes and every enzyme is coded for by a specific gene(s)  biochemical reactions do not occur in isolation but form part of a chain ...
Proteins
Proteins

... ADD ...
10.1 The function of Digestion pg. 402 Macromolecules and Living
10.1 The function of Digestion pg. 402 Macromolecules and Living

... occur in an organism. (Anabolic and Catabolic) Essential Nutrients – are nutrients that cannot be made by the body, and must therefore be obtained from food. Macromolecules are very important nutrients; raw materials that our bodies need to provide energy for regular cellular activities, build and r ...
Center for Structural Biology
Center for Structural Biology

...  Detoxification: Some amino acids are used detoxification reaction e.g glycine  Health and growth: Essential amino acids support growth in infants and maintain health in adults. ...
01 Structure, properties and biological functions of proteins
01 Structure, properties and biological functions of proteins

... with lipid. The plasma lipoproteins function primarily in the transport of lipids to sites of active membrane synthesis. Serum levels of low density lipoproteins (LDLs) are often used as a clinical index of susceptibility to vascular disease. Nucleoproteins. Nucleoprotein conjugates have many roles ...
The Macromolecular X-ray Crystallography (MX) ESRF Tutorial
The Macromolecular X-ray Crystallography (MX) ESRF Tutorial

... drugs. There are a number of methods for studying 3D structure. But we will concentrate on X-ray crystallography, which is one of the most effective at present. Other techniques complement effectively Xray crystallography and have a valued place in the set of tools that scientists can use. What is X ...
Cellular Gate Technology
Cellular Gate Technology

... reactions. Moreover, they reproduce themselves, allowing the creation of many copies with little manufacturing effort. The ability to control cellular function will provide important capabilities in computation, materials manufacturing, sensing, effecting, and fabrication at the molecular scale. Thi ...
A new type of Hidden Markov Models to predict complex domain
A new type of Hidden Markov Models to predict complex domain

... transitions between states. The core of a profile HMM (see the insert in Fig. 1) is a linear sequence of match (M ) states, one for each conserved position (consensus column) of a multiple alignment. Each M state emits (aligns to) a single residue, with a probability that is determined by the frequ ...
LEGO Activities
LEGO Activities

... 1. LEGO® stacks 2. Tape or markers and cardboard or butcher paper This activity can be as “fancy” or as simple as you are willing to make it. Students need to work both within their group, and collaborate with other groups, to create a gel using their LEGO® stacks. (If groups are very large, student ...
File S1.
File S1.

... software. You instructor will give you a short demo during a previous lab or in lecture. There are also many PyMol tutorials available online. For a basic tutorial, check out this YouTube video: http://youtu.be/RAftPWs1sWQ. Go to the PyMol website and download the Educational-use-only version of the ...
91.510_ch9_2
91.510_ch9_2

... Ab initio protein structure prediction Ab initio prediction can be performed when a protein has no detectable homologs. Protein folding is modeled based on global free-energy minimum estimates. The “Rosetta Stone” methods was applied to sequence families lacking known structures. For 80 of 131 prot ...
APP HIGHLIGHT—Rapid and efficient selection of high producing
APP HIGHLIGHT—Rapid and efficient selection of high producing

... to be selected that are high producers and secretors of therapeutic protein. Selecting colonies of transfected CHO and HEK293 cells secreting monomeric therapeutic proteins, using target-protein specific fluorescently labelled antibody, fluorescent imaging and robotic processing and picking techniqu ...
Protocol S4 – Clustering to define complexes, functional
Protocol S4 – Clustering to define complexes, functional

... GC (Protocols S5) and function prediction (Protocols S9) using the Markov Clustering (MCL) algorithm [1], following both biological and structural optimization. These networks are weighted networks, were edge’s weight represent the likelihoods for paired protein interactions in each network. Tuning ...
TRPA1 antibody - middle region (ARP35205_P050)
TRPA1 antibody - middle region (ARP35205_P050)

... family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (). Synthetic peptide located within the following region: KCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQ ...
Membrane Transport
Membrane Transport

... – collapsing ion gradients – facilitating entry by toxic agents – introducing a harmful catalytic activity ...
trypsin inhibitor and castor-bean (Ricinus communis) storage protein
trypsin inhibitor and castor-bean (Ricinus communis) storage protein

... 'highly significant' (Moore & Goodman, 1977; Vogel, 1978). This result strongly suggests a common ancestry for the two proteins, at least for the N-terminal 74 residues, and also suggests that the castor-bean storage protein has been synthesized as a single polypeptide precursor having the small sub ...
Protein Sulfenylation in Mitochondria: Biochemistry and
Protein Sulfenylation in Mitochondria: Biochemistry and

... protein modifications cysteines are less frequent in proteins than all other amino acids. If present, they are very often evolutionary conserved pointing at a functional relevance of the cysteine at these positions. Cysteine oxidation processes serve many different purposes but in general two diffe ...
PPT File
PPT File

... Bradykinin (9 a.a.): inhibits inflammation of tissues Thyrotropin-releasing factor (3 a.a.): formed in hypothalamus stimulates the release of thyrotropin from the anterior pituitary ...
Protein Structure Prediction Using Rosetta
Protein Structure Prediction Using Rosetta

... The accuracies ranged from as low as 3.9Å for 1BZ4 to as high as 12.5Å for 1CHD. Although very accurate models were generated for the smaller proteins, the remainder of models had a wide range of RMSD values. At the same time, the plots indicate that most of these high RMSD value models can be remov ...
File
File

... proteasome, a tunnel-like multiprotein structure – As the protein moves through the tunnel, it is straightened and dismantled – Proteasomes also destroy properly-folded proteins that are in excess or no longer needed ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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