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... number of specific RNA molecules that form part of the structure of a ribosome and participate in the synthesis of proteins • tRNA transfer RNA (tRNA) Set of small RNA molecules used in protein synthesis as an interface (adaptor) between messenger RNA and amino acids. ...
General Nutrient Requirements
General Nutrient Requirements

... them fast enough to meet demand – Arginine, histidine, isoleucine, leucine, threonine, lysine, methionine, phenylalanine, tryptophan, and valine ...
Assay the Activity of Alkaline Phosphatase (ALP) in Serum
Assay the Activity of Alkaline Phosphatase (ALP) in Serum

... 2) a large number of enzyme species are released from cells during normal cell turnover. ...
chap-4 - Workforce3One
chap-4 - Workforce3One

... PCR • Fluorescent-tagged oligonucleotide serves as a reporter probe – Fluorescent tag at 5’end – Fluorescence quenching tag at 3’end • With PCR rounds the 5’ tag is separated from the 3’ tag • Fluorescence increases with incorporation into DNA product ...
Practice Exam 3
Practice Exam 3

... Which enzyme catalyzes a reaction in which NADH is produced? _____________________ Which enzyme converts G3P into 1,3 BPG? __________________________ Name two enzyme reactions from glycolysis that operate at G ≈ 0 _______________________ 8. Three reactions in glycolysis operate far from equilibrium ...
The Effect of Detergents on Amino Acid Liberation by
The Effect of Detergents on Amino Acid Liberation by

... required in small quantities in the cell and is undetectable as a free-pool amino acid by the methods employed in this study. Several factors may contribute to tryptophan overproduction, either singly or in combination : deregulation of aromatic amino acid biosynthesis at the prime branch point of t ...
Practice Exam 3 Answers
Practice Exam 3 Answers

... Which enzyme catalyzes a reaction in which NADH is produced? _____________________ Which enzyme converts G3P into 1,3 BPG? __________________________ Name two enzyme reactions from glycolysis that operate at G ≈ 0 _______________________ 8. Three reactions in glycolysis operate far from equilibrium ...
lecture08_06
lecture08_06

Slide 1
Slide 1

... – Some antibiotics work better together than alone – Combining 2 or more drugs may be required to prevent the emergence of resistance e.g. tuberculosis – Combinations should not be given when 1 drug would suffice • Antagonistic effects • No ability to adjust 1 drug concentration ...
Reading Guide for Week 4
Reading Guide for Week 4

... between them. What is reducing power? 4. What are precursor metabolites and where do they come from? No need to memorize the names of the precursor metabolites, but you should understand that the central metabolic pathways produce them and that they are used to make subunits: Subunits (made from pre ...
Reading Guide for Week 4
Reading Guide for Week 4

... Subunits (made from precursor metabolites) amino acids nucleotides monosaccharides glycerol fatty acids ...
Triacylglycerol Metabolism Gone Bad: A major cause of disease
Triacylglycerol Metabolism Gone Bad: A major cause of disease

Chemistry of Metabolism
Chemistry of Metabolism

Analysis of Gene Sequences
Analysis of Gene Sequences

... (1) A crude preparation of chromosomal DNA is extracted from the bacterial strain of interest. (2) Two short oligo nucleotide primers (each about 18 bases long) are added to the DNA. The primers are designed from the known genomic sequence to be complimentary to opposite strands of DNA and to flank ...


... 2. Water sticks to other polar molecules (hydrophilic molecules) as well. This is called adhesion and is also a result of hydrogen bonding. This causes capillary action which also helps transport water in plants. Water is taken up by porous hydrophilic materials like paper or a sponge because of adh ...
Carbon Macromolecules
Carbon Macromolecules

... 2. One carbon atom can bond to another carbon atom, which gives carbon the ability to form chains that are almost unlimited in length. Carbon has the ability to form millions of different large and complex structures. ...
The Origins of Life and Precambrian Evolution
The Origins of Life and Precambrian Evolution

Amino Acids and Peptides
Amino Acids and Peptides

... • In the case of glycine two hydrogen atoms are bonded to the -carbon • In all other amino acids the the side chain is larger and more complex ...
Precipitation of Proteins at isoelectric Point
Precipitation of Proteins at isoelectric Point

... • The solubility of proteins in aqueous buffers depends on the distribution of hydrophilic and hydrophobic amino acid residues on the protein’s surface. Proteins that have high hydrophobic amino acid content on the surface have low solubility in an aqueous solvent. • Hydrophilic amino acid like (Arg ...
PASS MOCK EXAM
PASS MOCK EXAM

... b. Phospholipids favor flip motion rather than lateral motion 
 c. Cholesterol is equally distributed over both sides 
 d. Lipid self-assembly is an enzyme catalyzed process 
 34. Which class of membrane protein is incorrectly matched? a. Peripheral à can only be moved with detergent b. Lipid-ancho ...
m5zn_a9c640ccbe96115
m5zn_a9c640ccbe96115

... c) Myoglubin . d) Nothing . 51) Alpha helix differ from Beta sheet by……………………… a) H-bond exbend parallel to backbone . b) H-bond exbend vertical to backbone . c) Have only one polypeptide chain . d) A & C . 52) In alpha helix the H-bond performed between the carbonyl oxygen and.. a) Hydrogen of side ...
Chapter 3 part II
Chapter 3 part II

... Possible sources of probes No homologous DNA from another organism?  If amino acid sequence is known, deduce a set of nucleotide sequences to code for these amino acids  Construct these nucleotide sequences chemically using the synthetic probes  Why use several?  Genetic code is degenerate with ...
Authors` pre-proof version - University of Connecticut
Authors` pre-proof version - University of Connecticut

... syntax of the genetic code, upon which all protein synthesis depends. Aminoacylation of all 20 universal amino acids is partitioned between two major aaRS folds (class I and class II). Within each class, the aaRSs with different amino acid specificity show distant shared ancestry as revealed by stru ...
doc BIOL 112 Course Summary 2013
doc BIOL 112 Course Summary 2013

... Folding is crucial to the function of proteins o Influenced by the sequence of amino acids The alpha carbon in the amino acid is attached to an amino group, carboxyl group, and R group o R-group determines identity of the amino acid Peptide linkages are the covalent bonds between two amino acids Eve ...
Glycolipids and Glyc..
Glycolipids and Glyc..

... activity is found on the lumenal side of this membrane. The first two reactions transfer N-acetyl glucosamine onto dolichol-PP forming N,N'-Diacetylglucosamine diphosphodolichol (C04537). The remaining monosaccharide units are added sequentially by membrane bound transferases until the the dolichol ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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