Supporting material
... PaaI for Structural Characterization - The gene for paaI was cloned and expressed at Structural GenomiX, Inc (www.nysgxrc.org). To facilitate purification, paaI was amplified by PCR using Taq polymerase, cloned into a into a pET vector modified for topoisomerase directed cloning (Invitrogen) that wa ...
... PaaI for Structural Characterization - The gene for paaI was cloned and expressed at Structural GenomiX, Inc (www.nysgxrc.org). To facilitate purification, paaI was amplified by PCR using Taq polymerase, cloned into a into a pET vector modified for topoisomerase directed cloning (Invitrogen) that wa ...
Nucleic Acid and Protein Quantitation Methods
... detection. For dsDNA, the common methods include Hoechst, and Invitrogen Quant-iT PicoGreen®, Broad Range, and High Sensitivity dsDNA kits. These dyes have different excitation/emission profiles (Table 1) which may be more or less convenient depending on the individual application. Hoechst can be le ...
... detection. For dsDNA, the common methods include Hoechst, and Invitrogen Quant-iT PicoGreen®, Broad Range, and High Sensitivity dsDNA kits. These dyes have different excitation/emission profiles (Table 1) which may be more or less convenient depending on the individual application. Hoechst can be le ...
Alternative routes of acetyl-CoA synthesis identified
... generation of precursors for fatty acid and lipid synthesis, particularly acetyl-CoA, were then identified that were not present in the non-oleaginous strains. We found a set of the orthologous oleaginous proteins that was responsible for the biosynthesis of this key two-carbon metabolite through ci ...
... generation of precursors for fatty acid and lipid synthesis, particularly acetyl-CoA, were then identified that were not present in the non-oleaginous strains. We found a set of the orthologous oleaginous proteins that was responsible for the biosynthesis of this key two-carbon metabolite through ci ...
Document
... commonly addition or removal of oxygen or hydrogen. Because oxidation and reduction must occur together, these enzymes require coenzymes that are reduced or oxidized as the substrate is oxidized or reduced. ...
... commonly addition or removal of oxygen or hydrogen. Because oxidation and reduction must occur together, these enzymes require coenzymes that are reduced or oxidized as the substrate is oxidized or reduced. ...
University of Groningen Transport processes in penicillin
... activity against several species of Gram-positive bacteria [106]. Fleming termed the antimicrobial component penicillin, but did not isolate nor characterize it. Although frequently suggested, Fleming was certainly not the first to observe fungal antibiotic production [228]. The significance of Flem ...
... activity against several species of Gram-positive bacteria [106]. Fleming termed the antimicrobial component penicillin, but did not isolate nor characterize it. Although frequently suggested, Fleming was certainly not the first to observe fungal antibiotic production [228]. The significance of Flem ...
Sensing the fat: Fatty acid metabolism in the
... to body weight gain. Further work will be necessary to address these issues. 3. Fatty acid synthesis pathway in the hypothalamus In situations where total energy intake exceeds energy expenditure, fatty acids and triacylglycerols are synthesized and triacylglycerols are deposited in adipose tissue. ...
... to body weight gain. Further work will be necessary to address these issues. 3. Fatty acid synthesis pathway in the hypothalamus In situations where total energy intake exceeds energy expenditure, fatty acids and triacylglycerols are synthesized and triacylglycerols are deposited in adipose tissue. ...
Diapositiva 1 - UniFI
... Replacement of two 1H in each methyl group by 2H. Stereo-selective modification of the prochiral methyl groups of Leu and Val such that one methyl is 12C(2H)3 and the other is 13C1H(2H)2. ...
... Replacement of two 1H in each methyl group by 2H. Stereo-selective modification of the prochiral methyl groups of Leu and Val such that one methyl is 12C(2H)3 and the other is 13C1H(2H)2. ...
Nerve activates contraction
... from body cells to the liver for breakdown; are increased by exercise, and limited coffee, smoking, and saturated fats/trans fats Healthy Ratios in the Blood ...
... from body cells to the liver for breakdown; are increased by exercise, and limited coffee, smoking, and saturated fats/trans fats Healthy Ratios in the Blood ...
Amino Acid Catabolism: C
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
Amino Acid Catabolism: C
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
... Histidine is first converted to glutamate. The last step in this pathway involves the cofactor tetrahydrofolate. Tetrahydrofolate (THF), which has a pteridine ring, is a reduced form of the B vitamin folate. Within a cell, THF has an attached chain of several glutamate residues, linked to one anothe ...
Lab #8 Prelab: Protein, Triglycerides, and Esters Lab
... of the proteins in milk. It is interesting that proline (see figure), an unsymmetrical amino acids makes up 20% of the amino acids residues in some caseins. In proline, the side chain makes a ring structure with the amino group. The result is a lack of ordered internal protein secondary structure an ...
... of the proteins in milk. It is interesting that proline (see figure), an unsymmetrical amino acids makes up 20% of the amino acids residues in some caseins. In proline, the side chain makes a ring structure with the amino group. The result is a lack of ordered internal protein secondary structure an ...
The Nutritional Value of Brown Rice and Maize for Growing Pigs
... experiment were comparatively low. This result might be due to the following reasons. One reason may be that the fibre content in the maize was correlatively higher than that of brown rice and the balance of nonessential amino acids in maize was poorer. Another reason might be contributed by the low ...
... experiment were comparatively low. This result might be due to the following reasons. One reason may be that the fibre content in the maize was correlatively higher than that of brown rice and the balance of nonessential amino acids in maize was poorer. Another reason might be contributed by the low ...
Cloning and Expression Characteristics of the Pig Stra8 Gene
... The Stra8 gene is one of 50 RA-responsive genes isolated from a P19 pluripotent embryonal carcinoma (EC) cell line treated with RA. The expression of Stra8 in spermatogonia coincides with the transition from undifferentiated type A spermatogonia to differentiated type A1 spermatogonia. Stra8 cDNA (1 ...
... The Stra8 gene is one of 50 RA-responsive genes isolated from a P19 pluripotent embryonal carcinoma (EC) cell line treated with RA. The expression of Stra8 in spermatogonia coincides with the transition from undifferentiated type A spermatogonia to differentiated type A1 spermatogonia. Stra8 cDNA (1 ...
Structural and Biochemical Characterization of a Bifunctional
... catalyzed by a thymidylyltransferase (RmlA), involves the attachment of dTMP to glucose-1-phosphate. In the second step, the C-6′ hydroxyl group is removed, and the C-4′ hydroxyl group is converted to a keto moiety by the action of a 4,6-dehydratase (RmlB). These two steps are common to the biosynth ...
... catalyzed by a thymidylyltransferase (RmlA), involves the attachment of dTMP to glucose-1-phosphate. In the second step, the C-6′ hydroxyl group is removed, and the C-4′ hydroxyl group is converted to a keto moiety by the action of a 4,6-dehydratase (RmlB). These two steps are common to the biosynth ...
The Genetic Code
... codon – Only tryptophan and methionine are encoded by single codon (Figure 13-7) – Genetic code shows order: chemically similar amino acids share one or two middle bases in triplets encoding them ...
... codon – Only tryptophan and methionine are encoded by single codon (Figure 13-7) – Genetic code shows order: chemically similar amino acids share one or two middle bases in triplets encoding them ...
Cloning and Expression Characteristics of the Pig Stra8 Gene
... The Stra8 gene is one of 50 RA-responsive genes isolated from a P19 pluripotent embryonal carcinoma (EC) cell line treated with RA. The expression of Stra8 in spermatogonia coincides with the transition from undifferentiated type A spermatogonia to differentiated type A1 spermatogonia. Stra8 cDNA (1 ...
... The Stra8 gene is one of 50 RA-responsive genes isolated from a P19 pluripotent embryonal carcinoma (EC) cell line treated with RA. The expression of Stra8 in spermatogonia coincides with the transition from undifferentiated type A spermatogonia to differentiated type A1 spermatogonia. Stra8 cDNA (1 ...
25. biosynthesis of lipids
... meals while rebuilding glycogen reserves and converting any excess glucose to fatty acids. As the time of the last meal recedes, and glycogen supply again becomes depleted, more and more of his energy is obtained by oxidizing fatty acids previously stored as triglycerides. Even the overnight fast is ...
... meals while rebuilding glycogen reserves and converting any excess glucose to fatty acids. As the time of the last meal recedes, and glycogen supply again becomes depleted, more and more of his energy is obtained by oxidizing fatty acids previously stored as triglycerides. Even the overnight fast is ...
use cellular respiration
... Space between inner membrane & outer membrane INTERMEMBRANE SPACE = ____________________ Space inside cristae folds = _________________ MATRIX ...
... Space between inner membrane & outer membrane INTERMEMBRANE SPACE = ____________________ Space inside cristae folds = _________________ MATRIX ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.