A bacterial two-hybrid genome fragment library for
... interactions would probably be detected with higher efficacy in bacteria (even if the two-hybrid screen is performed in a heterologous host such as E. coli), in particular due to the possible cytotoxic nature of the produced proteins. Therefore, in the present study, we developed bacterial two-hybri ...
... interactions would probably be detected with higher efficacy in bacteria (even if the two-hybrid screen is performed in a heterologous host such as E. coli), in particular due to the possible cytotoxic nature of the produced proteins. Therefore, in the present study, we developed bacterial two-hybri ...
Diploidy and the selective advantage for sexual reproduction in
... 1995; Hurst and Peck 1996; Agrawal 2006; Visser and Elena 2007). The various theories for the selective advantage for sex fall into one of two general categories: The first category of theories argues that sex provides a mechanism to purge deleterious mutations from a genome (Kondrashov 1988; Muller ...
... 1995; Hurst and Peck 1996; Agrawal 2006; Visser and Elena 2007). The various theories for the selective advantage for sex fall into one of two general categories: The first category of theories argues that sex provides a mechanism to purge deleterious mutations from a genome (Kondrashov 1988; Muller ...
Powerpoint Presentation: Meiosis
... separate on the spindle Each cell will receive a copy of each chromosome type (i.e. it will receive n chromatids all different) The genes on the different chromosomes are recombined (shuffled) ...
... separate on the spindle Each cell will receive a copy of each chromosome type (i.e. it will receive n chromatids all different) The genes on the different chromosomes are recombined (shuffled) ...
Using Mice to Dissect Genetic Factors in Atherosclerosis
... least an order of magnitude greater than in human studies. Typically, the regions identified are relatively large and encompass several hundred genes across millions of base pairs. However, once the genes have been mapped to chromosomal regions, each of those particular genetic intervals ...
... least an order of magnitude greater than in human studies. Typically, the regions identified are relatively large and encompass several hundred genes across millions of base pairs. However, once the genes have been mapped to chromosomal regions, each of those particular genetic intervals ...
The Maintenance and Propagation of Plasmid Genes in Bacterial
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
The Maintenance and Propagation of Plasmid Genes in Bacterial
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
worksheet
... Icefish are a group of fish that belong to the Notothenioid suborder that lives in the most extreme Antarctic environments. Just how is it that icefish are able to tolerate the extreme conditions in which they live? What adaptations do they possess? Antifreeze Experiment: One of the key notothenioid ...
... Icefish are a group of fish that belong to the Notothenioid suborder that lives in the most extreme Antarctic environments. Just how is it that icefish are able to tolerate the extreme conditions in which they live? What adaptations do they possess? Antifreeze Experiment: One of the key notothenioid ...
Trichoderma reesei RUT-C30 – thirty years of strain
... nature of RUT-C30 with the absent genes. The growth of RUT-C30 was impaired on a-linked oligo- and polysaccharides such as dextrin, starch and maltose, which was thought to be due the absence of the gene encoding maltose permease responsible for a-glucoside uptake. It was also observed that conidia ...
... nature of RUT-C30 with the absent genes. The growth of RUT-C30 was impaired on a-linked oligo- and polysaccharides such as dextrin, starch and maltose, which was thought to be due the absence of the gene encoding maltose permease responsible for a-glucoside uptake. It was also observed that conidia ...
(a) (b)
... • For a few mammalian traits, the phenotype depends on which parent passed along the alleles for those traits • Such variation in phenotype is called genomic imprinting • Genomic imprinting involves the silencing of certain genes that are “stamped” with an ...
... • For a few mammalian traits, the phenotype depends on which parent passed along the alleles for those traits • Such variation in phenotype is called genomic imprinting • Genomic imprinting involves the silencing of certain genes that are “stamped” with an ...
- bioRxiv
... In contrast to work with single cell organisms, the impact of gene dosage on expression variability in metazoans is less well studied. However, a full understanding of the effect of gene copy deletions is fundamental for better understanding of diseases that originate from gene copy number changes. ...
... In contrast to work with single cell organisms, the impact of gene dosage on expression variability in metazoans is less well studied. However, a full understanding of the effect of gene copy deletions is fundamental for better understanding of diseases that originate from gene copy number changes. ...
Plant Genome Resources at the National Center for Biotechnology
... Solanum tuberosum, and V. vinifera. These alignments make an important link between EST data, which is relatively inexpensive to obtain, and assembled, wellannotated genomic sequence, a more expensive commodity. In addition to these ESTs, more than three million SNPs, submitted to NCBI’s dbSNP datab ...
... Solanum tuberosum, and V. vinifera. These alignments make an important link between EST data, which is relatively inexpensive to obtain, and assembled, wellannotated genomic sequence, a more expensive commodity. In addition to these ESTs, more than three million SNPs, submitted to NCBI’s dbSNP datab ...
Chromosomal Basis of Heredity
... • Under normal Mendelian genetic rules, we would not expect linked genes to recombine into assortments of alleles not found in the parents. • If the seed color and seed coat genes were linked, we would expect the F1 offspring to produce only two types of gametes, YR and yr when the tetrads separate. ...
... • Under normal Mendelian genetic rules, we would not expect linked genes to recombine into assortments of alleles not found in the parents. • If the seed color and seed coat genes were linked, we would expect the F1 offspring to produce only two types of gametes, YR and yr when the tetrads separate. ...
A Novel Two Domain-Fusion Protein in Cyanobacteria with
... Synechococcus OS-B’ (Synechococcus sp. JA-2–3B#a (2–13) Genbank NC_007776) was grown in DH-10 medium (i.e. Medium D, containing 10 mM HEPES pH 8.2) supplemented with Va vitamin solution used at 1000 dilution (Davis and Guillard, 1958; Kilian et al., 2007). The cultures were maintained at 52.5C and ...
... Synechococcus OS-B’ (Synechococcus sp. JA-2–3B#a (2–13) Genbank NC_007776) was grown in DH-10 medium (i.e. Medium D, containing 10 mM HEPES pH 8.2) supplemented with Va vitamin solution used at 1000 dilution (Davis and Guillard, 1958; Kilian et al., 2007). The cultures were maintained at 52.5C and ...
Project Title: Genetic Improvement of Photosynthetic Efficiency and
... associated with relevant developmental stages, total RNA samples will be collected and subjected to RNA seq based genome-wide profiling studies. From these genomic studies, in the context of genes specifically regulated differentially between the lines displaying photosynthetic efficiency compared t ...
... associated with relevant developmental stages, total RNA samples will be collected and subjected to RNA seq based genome-wide profiling studies. From these genomic studies, in the context of genes specifically regulated differentially between the lines displaying photosynthetic efficiency compared t ...
Chromosomal Microarray Analysis
... Assessment of Patient Understanding Patients should understand that: – CMA DOES NOT test for ALL genetic conditions – Detection rates are not 100% – Even if the results are normal, the baby could still have a birth defect(s) or mental retardation from causes not detected by the CMA testing ...
... Assessment of Patient Understanding Patients should understand that: – CMA DOES NOT test for ALL genetic conditions – Detection rates are not 100% – Even if the results are normal, the baby could still have a birth defect(s) or mental retardation from causes not detected by the CMA testing ...
High mutation rates in human and ape pseudoautosomal genes
... sequences by eye in ProSeq. The synonymous divergence (Ks) was calculated using MEGA (Kumar et al., 1994) using the method of Li et al. (1985). The data of Wolfe and Sharp (1993) were used to build the distribution of mouse/rat Ks values across the genome. To count the number of mutations of differe ...
... sequences by eye in ProSeq. The synonymous divergence (Ks) was calculated using MEGA (Kumar et al., 1994) using the method of Li et al. (1985). The data of Wolfe and Sharp (1993) were used to build the distribution of mouse/rat Ks values across the genome. To count the number of mutations of differe ...
Sequences of flavivirus-related RNA viruses persist in DNA form
... and Aedes aegypti mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the Aedes albopictus C6/36 cell line, an open reading frame (ORF) of 1557 aa with protease/helic ...
... and Aedes aegypti mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the Aedes albopictus C6/36 cell line, an open reading frame (ORF) of 1557 aa with protease/helic ...
Linkage, Recombination, and the Mapping of Genes on Chromosomes
... quite often. Geneticists can use data about how often genes separate during transmission to map the genes’ relative locations on a chromosome. Such mapping is a key to sorting out and tracking down the components of complex genetic networks; it is also crucial to geneticists’ ability to isolate and ...
... quite often. Geneticists can use data about how often genes separate during transmission to map the genes’ relative locations on a chromosome. Such mapping is a key to sorting out and tracking down the components of complex genetic networks; it is also crucial to geneticists’ ability to isolate and ...
Homology - a persona..
... sequences but not for the taxa from which they derive; a gene tree is not necessarily a species tree. Xenology is defined as that condition (horizontal transfer) where the history of the gene involves an interspecies transfer of genetic material12. It does not include transfer between organelles and ...
... sequences but not for the taxa from which they derive; a gene tree is not necessarily a species tree. Xenology is defined as that condition (horizontal transfer) where the history of the gene involves an interspecies transfer of genetic material12. It does not include transfer between organelles and ...
Polyploidy Enhances F Pollen Sterility Loci
... expressed genes and stage-specific gene expression. The complexity of gene expression during anther and ovary development has been revealed using microarrays or RNA sequencing in diploid rice (Tang et al., 2010; Aya et al., 2011; Deveshwar et al., 2011; Jin et al., 2013; Pan et al., 2014), maize (Zea ...
... expressed genes and stage-specific gene expression. The complexity of gene expression during anther and ovary development has been revealed using microarrays or RNA sequencing in diploid rice (Tang et al., 2010; Aya et al., 2011; Deveshwar et al., 2011; Jin et al., 2013; Pan et al., 2014), maize (Zea ...
Involvement of respiratory chain in biofilm formation in - HAL
... Both mutants were constructed and conirmed by RT-PCR and there was no polar effect on genes lanking the mutation. The mutations had no effect on bacterial growth in anaerobic condition as expected. However, these genes may be involved in bacterial growth under variable nutrients or in the presence o ...
... Both mutants were constructed and conirmed by RT-PCR and there was no polar effect on genes lanking the mutation. The mutations had no effect on bacterial growth in anaerobic condition as expected. However, these genes may be involved in bacterial growth under variable nutrients or in the presence o ...
Microbial Gene Transfer: An Ecological
... recent evidence from natural populations as well as genome research has indicated that this process may be a major driving force in microbial evolution. Studies with natural populations have taken two approacheseither adding a defined donor with a traceable gene to an indigenous community, and detec ...
... recent evidence from natural populations as well as genome research has indicated that this process may be a major driving force in microbial evolution. Studies with natural populations have taken two approacheseither adding a defined donor with a traceable gene to an indigenous community, and detec ...
Full text in pdf - International Microbiology
... K. variicola strains are more susceptible than K. pneumoniae to the antibiotics spectinomycin and gentamycin. At 250 mg spectinomycin/l, all of the K. variicola strains we tested were sensitive to the antibiotic, compared to only 28% of K. pneumoniae strains. Regarding gentamycin, 82% of the K. vari ...
... K. variicola strains are more susceptible than K. pneumoniae to the antibiotics spectinomycin and gentamycin. At 250 mg spectinomycin/l, all of the K. variicola strains we tested were sensitive to the antibiotic, compared to only 28% of K. pneumoniae strains. Regarding gentamycin, 82% of the K. vari ...
Identification and quantification of mycotoxigenic fungi
... There is a large literature on the subject. However, the basic process involves denaturing (separating the individual strands) DNA by heat applied for specific lengths of time. A small segment of DNA which can be referred to as a probe, is targeted to anneal with the piece of DNA of interest (the ta ...
... There is a large literature on the subject. However, the basic process involves denaturing (separating the individual strands) DNA by heat applied for specific lengths of time. A small segment of DNA which can be referred to as a probe, is targeted to anneal with the piece of DNA of interest (the ta ...
final1-eu-marie-curie-final-report
... assay. Studies by Gerwick and co-workers on the near complete genome of Moorea producens (L. majuscula), assert that the cyanobacterium does not contain niFH genes in its genome but is endowed with substantial amount of genes for microbial association. In our study we hypothesized that if any genes ...
... assay. Studies by Gerwick and co-workers on the near complete genome of Moorea producens (L. majuscula), assert that the cyanobacterium does not contain niFH genes in its genome but is endowed with substantial amount of genes for microbial association. In our study we hypothesized that if any genes ...