Martina Šeruga Musić, Mladen Krajačić, Dijana Škorić
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
... For direct amplification of phytoplasma gene or pseudogene regions, three different primer pairs were used: 16R738f/16R1232r (GIBB et al., 1995), to amplify a part of 16S rRNA gene; fTufu/rTufu (SCHNEIDER et al., 1997), to amplify a part of tuf gene; and G35p/m (DAVIS et al., 1992), to amplify dnaB ...
Article 1 Title: The pseudoautosomal regions of the U/V sex
... recombining region is usually not lost completely and it is thought that most species retain a PAR because homologous recombination in this region plays a critical role in chromosomal pairing and segregation during meiosis [4,5]. Moreover, there are situations where sexually antagonistic forces may ...
... recombining region is usually not lost completely and it is thought that most species retain a PAR because homologous recombination in this region plays a critical role in chromosomal pairing and segregation during meiosis [4,5]. Moreover, there are situations where sexually antagonistic forces may ...
Two enhancer regions in the mouse En-2 locus
... et al., 1991). In the adult, En-2 is strongly expressed in cells of the internal granular layer of the cerebellum. No expression is detected in the neighbouring Purkinje cells, whereas weak expression is detectable in cells of the molecular layer (Davis et al., 1988; Davis and Joyner, 1988; K. Mille ...
... et al., 1991). In the adult, En-2 is strongly expressed in cells of the internal granular layer of the cerebellum. No expression is detected in the neighbouring Purkinje cells, whereas weak expression is detectable in cells of the molecular layer (Davis et al., 1988; Davis and Joyner, 1988; K. Mille ...
MS-Word format
... 1c. How many total (direct and indirect) number of proteins are associated to the GO term polysaccharide metabolism? Are there any proteins which are not directly associated to this term? If so, which child term has the greatest number of associations? 1) In the same Summary page, examine the number ...
... 1c. How many total (direct and indirect) number of proteins are associated to the GO term polysaccharide metabolism? Are there any proteins which are not directly associated to this term? If so, which child term has the greatest number of associations? 1) In the same Summary page, examine the number ...
cis-Regulatory Elements and trans-Acting Factors
... an enhancer. Other studies have been performed using the JEG-3 cell line, which is derived from a human choriocarcinoma.17'18 However, this model has several limitations: these cells do not express the renin gene,18'19 and analysis of the 5'-flanking region of the gene required the use of the herpes ...
... an enhancer. Other studies have been performed using the JEG-3 cell line, which is derived from a human choriocarcinoma.17'18 However, this model has several limitations: these cells do not express the renin gene,18'19 and analysis of the 5'-flanking region of the gene required the use of the herpes ...
Association of Apolipoprotein E Alleles with Susceptibility to Age
... industrialized world. The public health burdens of AMDtend to increase since the senile populations begin to outnumber young in many countries (1-4). The etiopathology of AMD is not very well defined yet. However, it is believed that it is a multifactorial rmm.mazums.ac.ir ...
... industrialized world. The public health burdens of AMDtend to increase since the senile populations begin to outnumber young in many countries (1-4). The etiopathology of AMD is not very well defined yet. However, it is believed that it is a multifactorial rmm.mazums.ac.ir ...
A unified approach to the estimation and interpretation of resistance
... FRE-CNRS 3268, Université de Lille 1, Villeneuve d’Ascq Cedex, France ...
... FRE-CNRS 3268, Université de Lille 1, Villeneuve d’Ascq Cedex, France ...
Online resources for genetic variation study-Part One
... qualitatively. Affect gene translation quantitatively or qualitatively. Change protein structure and functions. Change gene regulation at different steps. ...
... qualitatively. Affect gene translation quantitatively or qualitatively. Change protein structure and functions. Change gene regulation at different steps. ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... with 50 PCOS subjects and reported 58% were heterozygous carriers of the polymorphic allele and 8% carried A2 allele in homozygosity, they concluded although this base pair substitution is not a primary genetic defect in PCOS , it may aggravate in clinical picture of hyerandogenemia, particularly wh ...
... with 50 PCOS subjects and reported 58% were heterozygous carriers of the polymorphic allele and 8% carried A2 allele in homozygosity, they concluded although this base pair substitution is not a primary genetic defect in PCOS , it may aggravate in clinical picture of hyerandogenemia, particularly wh ...
Applied and Environmental Microbiology
... region were cloned (32). A leucine-responsive regulatory protein (LRP)-like gene (lrpL), which is located immediately upstream of acdS, is required for the expression of ACC deaminase in 128C53K. Neither the acdS nor the lrpL gene knockout mutant of 128C53K produces ACC deaminase, and both of the mu ...
... region were cloned (32). A leucine-responsive regulatory protein (LRP)-like gene (lrpL), which is located immediately upstream of acdS, is required for the expression of ACC deaminase in 128C53K. Neither the acdS nor the lrpL gene knockout mutant of 128C53K produces ACC deaminase, and both of the mu ...
Chapter 14
... Pleiotropy: One Gene (pair) has Multiple Phenotypic Effects • Many genes have multiple phenotypic effects, a property called pleiotropy. This can be especially true for the gene (pair) responsible for genetic disease. ...
... Pleiotropy: One Gene (pair) has Multiple Phenotypic Effects • Many genes have multiple phenotypic effects, a property called pleiotropy. This can be especially true for the gene (pair) responsible for genetic disease. ...
Genes - Gerstein Lab Publications
... mutations that may lead to the acquisition of new functions, or to the modification of existing functions. However, some duplications of genes acquire deleterious mutations that disable them so that they can no longer be translated into a functioning protein. The disablement may occur at either or b ...
... mutations that may lead to the acquisition of new functions, or to the modification of existing functions. However, some duplications of genes acquire deleterious mutations that disable them so that they can no longer be translated into a functioning protein. The disablement may occur at either or b ...
Life Science - SC3206 IC Scope and Sequence
... Relate the genotypes of the offspring to their phenotypes. Advances in Genetics Compare the processes of selective breeding, cloning, and genetic engineering. Describe the impact of genetic technologies on society and the environment. Examine the use of gene therapy to treat disease. Evolution The T ...
... Relate the genotypes of the offspring to their phenotypes. Advances in Genetics Compare the processes of selective breeding, cloning, and genetic engineering. Describe the impact of genetic technologies on society and the environment. Examine the use of gene therapy to treat disease. Evolution The T ...
functional analysis of chromatin assembly genes in tetrahymena
... Amplification and molecular cloning of ~1Kb homologous sequences flanking up and ...
... Amplification and molecular cloning of ~1Kb homologous sequences flanking up and ...
MiSeqDx Cystic Fibrosis 139-Variant Assay Physician Insert
... Test results should be interpreted in the context of clinical findings, family history, and other laboratory data. Molecular testing may not detect all possible mutations leading to CF. A negative result does not rule out the possibility that the individual has an unidentified mutation in the CFTR g ...
... Test results should be interpreted in the context of clinical findings, family history, and other laboratory data. Molecular testing may not detect all possible mutations leading to CF. A negative result does not rule out the possibility that the individual has an unidentified mutation in the CFTR g ...
Chapter 13
... Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromoso ...
... Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromoso ...
A rapid and specific PCR–ELISA for detecting Salmonella typhi
... Background: Salmonella continues to be a major food borne pathogen for animals and humans. A sensitive and specific PCR–ELISA technique was developed to detect Salmonella typhi. Materials and methods: The assay was based on the incorporation of digoxigenin-labeled dUTP and a biotin-labeled primers s ...
... Background: Salmonella continues to be a major food borne pathogen for animals and humans. A sensitive and specific PCR–ELISA technique was developed to detect Salmonella typhi. Materials and methods: The assay was based on the incorporation of digoxigenin-labeled dUTP and a biotin-labeled primers s ...
Genetic Testing For Dilated Cardiomyopathy
... for low ejection fraction and/or other clinical symptoms are present. End-stage DCM can be treated with cardiac transplantation. Genetic DCM Genetic DCM has been proposed as a newer classification that includes both familial dilated cardiomyopathy and some cases of sporadic idiopathic dilated cardio ...
... for low ejection fraction and/or other clinical symptoms are present. End-stage DCM can be treated with cardiac transplantation. Genetic DCM Genetic DCM has been proposed as a newer classification that includes both familial dilated cardiomyopathy and some cases of sporadic idiopathic dilated cardio ...
Recommended Breeding Policy for the LaPerm cat - Kia-Ora
... Albino Series (Colourpoint, Burmese colour restriction) Dilute Diseases LaPerms are not at particular risk of any of the genetic diseases which can currently be tested for. However, some outcross breeds used in LaPerm breeding programmes do have genetic diseases identified in their gene pools wh ...
... Albino Series (Colourpoint, Burmese colour restriction) Dilute Diseases LaPerms are not at particular risk of any of the genetic diseases which can currently be tested for. However, some outcross breeds used in LaPerm breeding programmes do have genetic diseases identified in their gene pools wh ...
Plant Functional Genomics Plant Functional Genomics
... procedures efficient and easy to perform. Recombinant DNA inserts up to 200 kb can be efficiently cloned and stably maintained in E. coli. Although the insert size cloning capacity is much lower than that of the YAC system, it is this limited cloning capacity that helps to prevent chimerism, because ...
... procedures efficient and easy to perform. Recombinant DNA inserts up to 200 kb can be efficiently cloned and stably maintained in E. coli. Although the insert size cloning capacity is much lower than that of the YAC system, it is this limited cloning capacity that helps to prevent chimerism, because ...
Print - Physiological Genomics
... of detecting maternal effect genes affecting embryo and endosperm development in higher plants. The current molecular dissection of endosperm is mostly based on the assumption that endosperm-specific traits are only controlled by genes from the maternal sporophyte (22, 23). With this assumption (15) ...
... of detecting maternal effect genes affecting embryo and endosperm development in higher plants. The current molecular dissection of endosperm is mostly based on the assumption that endosperm-specific traits are only controlled by genes from the maternal sporophyte (22, 23). With this assumption (15) ...
Using Genetic Algorithms with Asexual Transposition
... certain genetic elements in corn occasionally move producing kernels with unusual colors that could not have resulted from crossover or mutation. Transposons were for a long time considered as some sort of abnormality, but in 1983, when she was awarded the Nobel Prize, many such transposons had been ...
... certain genetic elements in corn occasionally move producing kernels with unusual colors that could not have resulted from crossover or mutation. Transposons were for a long time considered as some sort of abnormality, but in 1983, when she was awarded the Nobel Prize, many such transposons had been ...
An evaluation of codes more compact than the natural genetic code
... Let us re-examine the first two positions of the natural synonymous codons. One popular theory24 speculates that a genetic code, table 5. Only seven of the 20 amino acids can doublet genetic code may have preceded the current triplet actually be associated unambiguously to a doublet. There are one. ...
... Let us re-examine the first two positions of the natural synonymous codons. One popular theory24 speculates that a genetic code, table 5. Only seven of the 20 amino acids can doublet genetic code may have preceded the current triplet actually be associated unambiguously to a doublet. There are one. ...
Genetic engineering
Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.