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Lab Test 1 Study Sheet (summer term)
Lab Test 1 Study Sheet (summer term)

... Have an understanding of the following topics: 24. Be able to describe the surface area to volume ratio of cells. 25. Recognize a cheek cell, and know some of the typical cellular organelles. 26. Know the differences in light microscope and electron scope. 27. What are the steps of the scientific me ...
Movement of Ions and Electrogenesis in Microorganisms
Movement of Ions and Electrogenesis in Microorganisms

HIV-1 Infection of Nondividing Cells: C-Terminal
HIV-1 Infection of Nondividing Cells: C-Terminal

... Taken together, these results demonstrate that myristoylation acts as a dominant targeting signal for MA during viral particle formation, yet for 1% of the molecules, it becomes recessive once HIV-1 enters target cells. This suggests a model in which some modification of MA, during or after assembly ...
Immunoglobulin Heavy Chain and Binding Protein Complexes Are
Immunoglobulin Heavy Chain and Binding Protein Complexes Are

... to demonstrate with any certainty that these proteins represent folding or assembly intermediates which will eventually be secreted and not aberrant dead-end products that are degraded. This has led to divergent hypotheses as to whether BiP functions primarily to bind defective proteins in the ER an ...
liposomes
liposomes

... • Liposomes are used as models for artificial cells. Liposomes can also be designed to deliver drugs in other ways. Liposomes that contain low (or high) pH can be constructed such that dissolved aqueous drugs will be charged in solution (i.e., the pH is outside the drug's pI range). As the pH natur ...
Vesicle traffic in the endomembrane system: a tale of COPs, Rabs
Vesicle traffic in the endomembrane system: a tale of COPs, Rabs

... are found in the endomembrane systems of all higher eukaryotes, a variety of specific adaptations are found only in certain lineages. For example, plants contain not only lytic vacuoles (LVs) but also protein storage vacuoles (PSVs) that perform radically different functions [1]. By ...
Team Teaching Project
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... At the beginning of the class all students are given a skeletal version handout of the PowerPoint to fill out during the presentation. The SE teacher will operate the computer and narrate and explain the Powerpoint presentation, while the GE teacher will circulate among the students to observe progr ...
Supplementary Figures 1–2 and Table (ppt 202K)
Supplementary Figures 1–2 and Table (ppt 202K)

... ER calcium stores, had only a slight effect on the flux. Since thapsigargin seems to strongly inhibit the uptake of apoptotic cells in the engulfment assay (Fig. 3a), this suggests that perhaps the plate calcium flux assay we used is not sensitive enough to detect changes in calcium flux due to intr ...
as-1-2-3-plant-revision
as-1-2-3-plant-revision

... • Provide large surface area as are very long extensions and occur in thousands on each root branch. • Have thin surface layer (cell surface membrane and cellulose cell wall), across which materials can move easily. ...
PHD domains and E3 ubiquitin ligases: viruses make the connection
PHD domains and E3 ubiquitin ligases: viruses make the connection

... degradation of MHC class I molecules [9,10], it soon became clear that this was not the mechanism by which the KSHV MIR proteins function. MHC class I chains in MIR-expressing cells exit the ER and arrive at the plasma membrane with normal kinetics, but are subsequently internalized into vesicular s ...
A New Paradigm in Cellular Biology Gerson, Ling, Cope, Sodi
A New Paradigm in Cellular Biology Gerson, Ling, Cope, Sodi

... Around the initial layer of highly structured water molecules is a second layer, which is less  structures because it is farther from the attractive force of the protein/lipid macromolecules. The third  layer is less structured than the second and so on. It is not known exactly how many layers of st ...
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PDF

... mitotic ER clearance from chromosomes both depend on a short, positively charged amino acid sequence connecting the two hydrophobic domains of REEP4. Our results show that REEP3/4 function redundantly to clear the ER from metaphase chromatin, thereby ensuring correct progression through mitosis and ...
Rabbit (polyclonal) Anti-Src pan Antibody, Unconjugated
Rabbit (polyclonal) Anti-Src pan Antibody, Unconjugated

... and G-CSF) and integrins. Src also regulates the activity of several ion channels including the Nmethyl-D-aspartate (NMDA) receptor. In addition, Src is thought to play a role in physiological/pathophysiological processes in the central nervous system. This antibody is useful to determine total leve ...
Membrane trafficking in Drosophila wing and eye development
Membrane trafficking in Drosophila wing and eye development

... acts primarily by inhibiting cells within a field from adopting a particular fate, a process called lateral inhibition. In the ‘core’ Notch pathway ligands, such as Delta and Serrate in Drosophila, interact with extracellular EGF motifs of the Notch receptor. Activation of Notch leads to proteolytic ...
The Protoplast: Plasma Membrane, Nucleus, and Cytoplasmic
The Protoplast: Plasma Membrane, Nucleus, and Cytoplasmic

... Among the internal membranes of eukaryotic cells are those surrounding the nucleus, mitochondria, and plastids, which are characteristic components of plant cells. The cytoplasm of eukaryotic cells also contains systems of membranes (the endoplasmic reticulum and Golgi apparatus) and a complex netwo ...
Dialysis Diffusion Lab
Dialysis Diffusion Lab

... Dialysis Diffusion/Osmosis Lab Introduction: Cells can use the principles of osmosis and diffusion to move molecules in and out of the cell without expending energy. Internal environments of cells are affected by the concentrations of fluids surrounding them and the concentrations of their interior ...
Reproduce It has the ability to produce offsprings by means of
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... There is no specific paramecium reproduction cycle as such. In favorable conditions, paramecium can undergo asexual multiplication for at least three times a day. Besides increased rate of multiplication, some paramecium strains form symbiotic association with bacteria and algae, which again enhance ...
1. Small Scale Expression of Tagged Recombinant Proteins
1. Small Scale Expression of Tagged Recombinant Proteins

... 1. Thaw the required number of tubes of cells on ice and mix gently to ensure that the cells are evenly suspended. Transform also with empty vector. 2. Add 1 μl of the DNA solution directly to the cells. Stir gently to mix. 3. Place the tubes on ice for 5 min. 4. Heat the tubes for exactly 30 s in a ...
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video slide

... Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings ...
The Cell Cycle Notes from Pearson
The Cell Cycle Notes from Pearson

... Cytokinesis completes the M phase of the cell cycle. It may begin while telophase is still taking place. During cytokinesis, the cytoplasm (which includes all of the contents of a eukaryotic cell outside the nucleus) draws inward, eventually pinching off into two nearly equal parts. Each part contai ...
C9orf72 binds SMCR8, localizes to lysosomes and regulates
C9orf72 binds SMCR8, localizes to lysosomes and regulates

... While not showing the lysosome clustering that was evident in the C9orf72 KO cells, the SMCR8 KO cells appeared significantly larger than either the wild type or C9orf72 KOs (Fig. 4B and 5A) and this difference was robustly supported by quantification of cellular footprint area (Fig. 5B). Furthermo ...
Hin- und Rückflug: €199,37
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... dynamics and mediate the anchorage of microtubules to different cellular structures, including kinetochores and membrane compartments. As such, they play important roles in all microtubule-based cellular processes. +TIPs comprise a structurally and functionally diverse group of multidomain and/or mu ...
Adaptively Sampled Distance Fields
Adaptively Sampled Distance Fields

... // cell and the total object’s surface area for (areaObject = 0, level = 0 to maxADFLevel) nCellsAtLevel = getNumBoundaryLeafCellsAtLevel(adf, level) areaCell[level] = sqr(cellSize(level)) areaObject += nCellsAtLevel * areaCell[level] // nPtsCell is proportional to areaCell / areaObject for (level = ...
Tour of Cell Organelles
Tour of Cell Organelles

...  Everything inside the cell between the cell membrane and the nucleus; consists of semi - fluid and organelles. ...
Mitochondrial inheritance is mediated by microtubules in
Mitochondrial inheritance is mediated by microtubules in

... morphology and distribution of mitochondria was previously thought to facilitate the stochastic inheritance of mitochondria by daughter cells. In contrast, we recently reported that mitochondria in dividing mammalian cells are inherited by an ordered mechanism of inheritance mediated by microtubules ...
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Cytosol



The cytosol or intracellular fluid (ICF) or cytoplasmic matrix is the liquid found inside cells. It is separated into compartments by membranes. For example, the mitochondrial matrix separates the mitochondrion into many compartments.In the eukaryotic cell, the cytosol is within the cell membrane and is part of the cytoplasm, which also comprises the mitochondria, plastids, and other organelles (but not their internal fluids and structures); the cell nucleus is separate. In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space. In eukaryotes, while many metabolic pathways still occur in the cytosol, others are contained within organelles.The cytosol is a complex mixture of substances dissolved in water. Although water forms the large majority of the cytosol, its structure and properties within cells is not well understood. The concentrations of ions such as sodium and potassium are different in the cytosol than in the extracellular fluid; these differences in ion levels are important in processes such as osmoregulation, cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The cytosol also contains large amounts of macromolecules, which can alter how molecules behave, through macromolecular crowding.Although it was once thought to be a simple solution of molecules, the cytosol has multiple levels of organization. These include concentration gradients of small molecules such as calcium, large complexes of enzymes that act together to carry out metabolic pathways, and protein complexes such as proteasomes and carboxysomes that enclose and separate parts of the cytosol.
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