pjp6`2001.vp:CorelVentura 7.0 - Institute of Pharmacology
... not show GR-immunopositive material. Above data are in sharp contrast to the data collected from the locus coeruleus, which has been used as a positive control, where we observed a clear co-localization of GR with TH. In conclusion, the obtained results indicate that dopaminergic neurons of the rat ...
... not show GR-immunopositive material. Above data are in sharp contrast to the data collected from the locus coeruleus, which has been used as a positive control, where we observed a clear co-localization of GR with TH. In conclusion, the obtained results indicate that dopaminergic neurons of the rat ...
Chapter 3
... • Neurotransmitters released into the synapse do not remain and are subject to either inactivation or reuptake. • Reuptake refers to when the presynaptic neuron takes up most of the neurotransmitter molecules intact and reuses it. • Transporters are special membrane proteins that facilitate reuptake ...
... • Neurotransmitters released into the synapse do not remain and are subject to either inactivation or reuptake. • Reuptake refers to when the presynaptic neuron takes up most of the neurotransmitter molecules intact and reuses it. • Transporters are special membrane proteins that facilitate reuptake ...
Hungry for Power: Elimination of Mitochondria by Mitophagy
... stressed organelles from the greater mitochondrial network for degradation, as perturbation of either of these genes results in swollen mitochondrial morphology in cells. Future Research While the cooperation of such sensing and induction mechanisms offers an attractive model of how mitophagy occurs ...
... stressed organelles from the greater mitochondrial network for degradation, as perturbation of either of these genes results in swollen mitochondrial morphology in cells. Future Research While the cooperation of such sensing and induction mechanisms offers an attractive model of how mitophagy occurs ...
Direct Visualization by Cryo-EM of the Mycobacterial Capsular
... proteins such as GroEL2, DnaK and translation elongation factor Tu (Table S2 and S3). The presence of some of these proteins in the capsule was also recently described by Hickey et al. [29]. In addition to these predominantly cytosolic proteins only very small amounts of putative capsular proteins, ...
... proteins such as GroEL2, DnaK and translation elongation factor Tu (Table S2 and S3). The presence of some of these proteins in the capsule was also recently described by Hickey et al. [29]. In addition to these predominantly cytosolic proteins only very small amounts of putative capsular proteins, ...
Theory_2004
... One gram of fat releases over twice as much energy than the same amount of glycogen The total mass of fat in the human body is about 1000-fold higher than that the total mass of body glycogen If all our energy was stored as glycogen, we would weigh at least 40 kg more than we do now Fat, but not gly ...
... One gram of fat releases over twice as much energy than the same amount of glycogen The total mass of fat in the human body is about 1000-fold higher than that the total mass of body glycogen If all our energy was stored as glycogen, we would weigh at least 40 kg more than we do now Fat, but not gly ...
Differential Expression of Four Connexin Genes, Cx-26, Cx
... Al’ JUNCTIONS are specialized plasma membrane associations containing intercellular channels that coordinate tissue function by allowing cell to cell passage of ions and small molecules (l-3). Gap junction channels (GJC) are formed by the association of two hemichannels (connexons), one provided by ...
... Al’ JUNCTIONS are specialized plasma membrane associations containing intercellular channels that coordinate tissue function by allowing cell to cell passage of ions and small molecules (l-3). Gap junction channels (GJC) are formed by the association of two hemichannels (connexons), one provided by ...
Anti-c-myc antibody 9E10 - Protein Engineering, Design and Selection
... intensity of the Western blots was visually compared. The Western blot analysis was carried out using 17% SDS polyacrylamide gels and blotting onto polyvinylidene fluoride (PVDF) membranes. After blocking the membrane for 1 h with 5% dry milk in TTBS and three times washing with TTBS the membrane wa ...
... intensity of the Western blots was visually compared. The Western blot analysis was carried out using 17% SDS polyacrylamide gels and blotting onto polyvinylidene fluoride (PVDF) membranes. After blocking the membrane for 1 h with 5% dry milk in TTBS and three times washing with TTBS the membrane wa ...
CDC46/MCM5, a yeast protein whose subcellular localization is cell cycle-regulated, is involved in DNA replication at autonomously replicating sequences.
... containing YCpUM2, and the transformants were streaked on Cm-ura-leu plates. Twelve of these transformants were restreaked onto Cm-leu+FOA plates to select against cells carrying the YCpUM2 plasmid. Cells can grow on Cm-leu+FOA plates only if CDC46 complements mcm2 or mcm4. The result was that no co ...
... containing YCpUM2, and the transformants were streaked on Cm-ura-leu plates. Twelve of these transformants were restreaked onto Cm-leu+FOA plates to select against cells carrying the YCpUM2 plasmid. Cells can grow on Cm-leu+FOA plates only if CDC46 complements mcm2 or mcm4. The result was that no co ...
Document
... • Transmission Across a Synapse – A synapse is a region where neurons nearly touch – Small gap between neurons is the synaptic cleft – Transmission across a synapse is carried out by neurotransmitters • Sudden rise in calcium in the axon terminal of one neuron • Calcium stimulates synaptic vesicles ...
... • Transmission Across a Synapse – A synapse is a region where neurons nearly touch – Small gap between neurons is the synaptic cleft – Transmission across a synapse is carried out by neurotransmitters • Sudden rise in calcium in the axon terminal of one neuron • Calcium stimulates synaptic vesicles ...
The A6B4 Integrin Can Regulate ErbB-3 Expression: Implications for
... between the endogenous a6 integrin and the exogenous h4 subunits in both parental and ErbB-2–expressing NIH 3T3 cells (Fig. 1C, top). The expression of the a6 subunit and the h4 subunit with a deleted cytoplasmic domain (h4L) was assessed by FACS analysis (Fig. 1D). The NIH 3T3 clones we used expres ...
... between the endogenous a6 integrin and the exogenous h4 subunits in both parental and ErbB-2–expressing NIH 3T3 cells (Fig. 1C, top). The expression of the a6 subunit and the h4 subunit with a deleted cytoplasmic domain (h4L) was assessed by FACS analysis (Fig. 1D). The NIH 3T3 clones we used expres ...
View Full PDF - Biochemical Society Transactions
... produce a secondary metabolite, now known as rapamycin, with potent antifungal activity [1,2]. Not long after its initial characterization as an antifungal agent, rapamycin was found to possess cytostatic activity not only against lower eukaryotes but also against mammalian cells, particularly immun ...
... produce a secondary metabolite, now known as rapamycin, with potent antifungal activity [1,2]. Not long after its initial characterization as an antifungal agent, rapamycin was found to possess cytostatic activity not only against lower eukaryotes but also against mammalian cells, particularly immun ...
Protein Structure Similarity
... Ab Initio Methods- These techniques attempt to determine protein structure from scratch by finding the global minimum of an energy function defined on the space of possible structural conformations of the protein. With present methods these techniques are extremely computationally costly and thus ha ...
... Ab Initio Methods- These techniques attempt to determine protein structure from scratch by finding the global minimum of an energy function defined on the space of possible structural conformations of the protein. With present methods these techniques are extremely computationally costly and thus ha ...
Lactate Receptor Sites Link Neurotransmission
... located with glutamate receptors at the postsynaptic membranes of fast acting excitatory synapses (Bergersen et al. 2001, 2005). Further, lactate is known to mediate cerebral vasodilatation causing increased brain blood flow (Gordon et al. 2008). The notion of multiple signaling roles of lactate in b ...
... located with glutamate receptors at the postsynaptic membranes of fast acting excitatory synapses (Bergersen et al. 2001, 2005). Further, lactate is known to mediate cerebral vasodilatation causing increased brain blood flow (Gordon et al. 2008). The notion of multiple signaling roles of lactate in b ...
GENERATION OF K581A MUTATION AND PRODUCTION OF RECOMBINANT JAK2 PROTEIN
... Protein kinases (PKs) are a large group of enzymes which catalyze transfer of a phosphate group, usually from ATP to the hydroxyl group of tyrosine, threonine or serine residue of their substrates (Choreschi K. et al. 2009) Vertebrate genome consist s of approximately 2000 different protein kinase ...
... Protein kinases (PKs) are a large group of enzymes which catalyze transfer of a phosphate group, usually from ATP to the hydroxyl group of tyrosine, threonine or serine residue of their substrates (Choreschi K. et al. 2009) Vertebrate genome consist s of approximately 2000 different protein kinase ...
Investigating regulation of aging by transcription factors DAF 16 and
... • Aging in eukaryotic organisms is a deleterious process • molecular pathways of aging and regulation • studies are conducted on model invertebrate organisms such as Caenorhabditis elegans (C. elegans) ...
... • Aging in eukaryotic organisms is a deleterious process • molecular pathways of aging and regulation • studies are conducted on model invertebrate organisms such as Caenorhabditis elegans (C. elegans) ...
Geology - Deep below North America
... to ATR suggesting one strategy for preventing toxin entry (assuming that ATR is the only cellular receptor). Pannifer and colleagues (2) have refined the crystal structure of LF to 2.2 Å resolution. Of LF’s four domains, domain I contains a docking site for PA, and domains II and III interact with d ...
... to ATR suggesting one strategy for preventing toxin entry (assuming that ATR is the only cellular receptor). Pannifer and colleagues (2) have refined the crystal structure of LF to 2.2 Å resolution. Of LF’s four domains, domain I contains a docking site for PA, and domains II and III interact with d ...
The neuron Label the following terms: Soma Axon terminal Axon
... 1. The presynaptic neuron sends neurotransmitters to postsynaptic neuron. 2. Neurotransmitters bind to receptors on the postsynaptic cell. - This action will either excite or inhibit the postsynaptic cell. - The soma becomes more positive. 3. The positive charge reaches the axon hillock. - Once the ...
... 1. The presynaptic neuron sends neurotransmitters to postsynaptic neuron. 2. Neurotransmitters bind to receptors on the postsynaptic cell. - This action will either excite or inhibit the postsynaptic cell. - The soma becomes more positive. 3. The positive charge reaches the axon hillock. - Once the ...
Dictyostelium morphogenesis
... ing the periodic movement of the cells forward (Figure 1). These optical waves are strictly dependent on the tip. Cells in the tip often rotate perpendicularly to the direction of slug migration, especially when it is lifted from the substrate. In the posterior part of the slug, the cells move forwa ...
... ing the periodic movement of the cells forward (Figure 1). These optical waves are strictly dependent on the tip. Cells in the tip often rotate perpendicularly to the direction of slug migration, especially when it is lifted from the substrate. In the posterior part of the slug, the cells move forwa ...
lecturenotes.pdf
... channel in place within the membrane. (Amino acids 1 - 400 and 850 1200). They also form the walls of the ion channel. Note in the figure on the right that the transmembrane regions are actually placed so they form the walls of the ion channel when shown three dimensionally. • Two nucleotide binding ...
... channel in place within the membrane. (Amino acids 1 - 400 and 850 1200). They also form the walls of the ion channel. Note in the figure on the right that the transmembrane regions are actually placed so they form the walls of the ion channel when shown three dimensionally. • Two nucleotide binding ...
EMBO Workshop on Cell Size Regulation
... Cell size regulation in higher eukaryotes (8:30-10:30) Alison Lloyd – Regulation of mammalian cell size Robert Sablowski - Coordination between cell size, cell cycle and organ patterning in plants Short talk – Clotilde Cadart - Coordination of cell cycle progression and cell growth in animal c ...
... Cell size regulation in higher eukaryotes (8:30-10:30) Alison Lloyd – Regulation of mammalian cell size Robert Sablowski - Coordination between cell size, cell cycle and organ patterning in plants Short talk – Clotilde Cadart - Coordination of cell cycle progression and cell growth in animal c ...
TAK1-binding protein 1 is a pseudophosphatase
... on ice for 30 min and brief sonication, the lysate was cleared by centrifugation at 29 000 g for 30 min followed by incubation for 1 h at 4 ◦C with 7.5 ml of packed glutathione–Sepharose beads (Amersham Biosciences). The resin was washed with 200 ml of lysis buffer [50 mM Tris/HCl (pH 7.5), 500 mM N ...
... on ice for 30 min and brief sonication, the lysate was cleared by centrifugation at 29 000 g for 30 min followed by incubation for 1 h at 4 ◦C with 7.5 ml of packed glutathione–Sepharose beads (Amersham Biosciences). The resin was washed with 200 ml of lysis buffer [50 mM Tris/HCl (pH 7.5), 500 mM N ...
Biologically active peptides in molluscs
... in the neuroendocrine, immune and gut system. Moreover, in gastropods the entire periphery of the nervous system, including nerves, connectives and commissure is used for hormone release. The central nervous system (CNS) of the freshwater snails Lymnaea stagnalis and Planorbarius corneus have been t ...
... in the neuroendocrine, immune and gut system. Moreover, in gastropods the entire periphery of the nervous system, including nerves, connectives and commissure is used for hormone release. The central nervous system (CNS) of the freshwater snails Lymnaea stagnalis and Planorbarius corneus have been t ...
The Role of Cdkn1A-Interacting Zinc Finger Protein 1 (CIZ1) in DNA
... Studies indicated that this domain might be related to protein interacting ability [20] and stability [21]. Studies indicated that this domain might be related to protein interacting ability [20] and stability [21]. The MH3 domain lies between 726 and 779 aa residues of CIZ1 [1]. This domain has bee ...
... Studies indicated that this domain might be related to protein interacting ability [20] and stability [21]. Studies indicated that this domain might be related to protein interacting ability [20] and stability [21]. The MH3 domain lies between 726 and 779 aa residues of CIZ1 [1]. This domain has bee ...
RNA binding proteins: a common denominator of neuronal function
... been shown to be indispensable for several aspects of neurogenesis, neurite outgrowth, synapse formation, and plasticity. Consequently, perturbation of their function is central in the etiology of an ever-growing spectrum of neurological diseases, including fragile X syndrome and the neurodegenerati ...
... been shown to be indispensable for several aspects of neurogenesis, neurite outgrowth, synapse formation, and plasticity. Consequently, perturbation of their function is central in the etiology of an ever-growing spectrum of neurological diseases, including fragile X syndrome and the neurodegenerati ...
Axon Guidance
... • A single cue can be attractive or repellent, depending on the growth cone response. • In developing limbs targeting of axons to dorsal vs ventral targets depends on neuronal expression of receptors and spatial expression of guidance cues. • At the midline, spatial and temporal regulation of exp ...
... • A single cue can be attractive or repellent, depending on the growth cone response. • In developing limbs targeting of axons to dorsal vs ventral targets depends on neuronal expression of receptors and spatial expression of guidance cues. • At the midline, spatial and temporal regulation of exp ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.