Metabolic Incorporation of Stable Isotope Labels into Glycans
... with amino acids in cell culture (SILAC), which provides an elegant method for the incorporation of an isotopic label into proteins prior to MS-based proteomics.18 In a SILAC experiment, two cell populations are grown in culture media that are identical except that one of them contains a “light”(nat ...
... with amino acids in cell culture (SILAC), which provides an elegant method for the incorporation of an isotopic label into proteins prior to MS-based proteomics.18 In a SILAC experiment, two cell populations are grown in culture media that are identical except that one of them contains a “light”(nat ...
Elg1 forms an alternative RFC complex important for DNA
... PCNA acts as a processivity factor for DNA polymerase d on the leading and lagging strands. At least two alternative forms of RFC have been identi®ed recently in yeast and humans. In the ®rst of these, the large subunit of RFC, Rfc1, is replaced by the Rad24 protein (Green et al., 2000). Rad24-RFC l ...
... PCNA acts as a processivity factor for DNA polymerase d on the leading and lagging strands. At least two alternative forms of RFC have been identi®ed recently in yeast and humans. In the ®rst of these, the large subunit of RFC, Rfc1, is replaced by the Rad24 protein (Green et al., 2000). Rad24-RFC l ...
Stem cells in the light of evolution
... Stem cells and multicellularity The distribution of stem cells has been recorded throughout the animal and plant kingdoms, and all these organisms are known to be multicellular. However, during the development of multicellular organisms through natural selection, stem cells accompanied the evoluti ...
... Stem cells and multicellularity The distribution of stem cells has been recorded throughout the animal and plant kingdoms, and all these organisms are known to be multicellular. However, during the development of multicellular organisms through natural selection, stem cells accompanied the evoluti ...
Probing cytoplasmic viscosity in the confined geometry of
... curve is rescaled to fit the experimental data, and that rescaling can be used to calculate the diffusion coefficient. While a powerful technique, these approaches rely on the time-scale-invariance of the process, which is not true in the case where the bleaching and imaging times cannot be consider ...
... curve is rescaled to fit the experimental data, and that rescaling can be used to calculate the diffusion coefficient. While a powerful technique, these approaches rely on the time-scale-invariance of the process, which is not true in the case where the bleaching and imaging times cannot be consider ...
PPT - Yavapai College
... Fermentation is oxygen-free respiration where organic molecules are ultimate electron acceptor. Many groups of bacteria are fermenters. ...
... Fermentation is oxygen-free respiration where organic molecules are ultimate electron acceptor. Many groups of bacteria are fermenters. ...
Molecular basis of differential gene expression in the mouse
... in unfertilized eggs is lost between fertilization and the late 2-cell stage, although there is little, if any, difference in total RNA content (Piko and Clegg, 1982; Hamatani et al., 2004). This indicates that much of the oocyte mRNA was for the purposes of oogenesis and the early stages of post-fe ...
... in unfertilized eggs is lost between fertilization and the late 2-cell stage, although there is little, if any, difference in total RNA content (Piko and Clegg, 1982; Hamatani et al., 2004). This indicates that much of the oocyte mRNA was for the purposes of oogenesis and the early stages of post-fe ...
Thiosemicarbazone Metal Complexes: From Structure to Activity
... phase of the cell cycle and eventually to cell death by apoptosis. They also indirectly demonstrated that the active species was the iron(II) complex of 1-formylisoquinoline thiosemicarbazone. In fact, it was later discovered that iron and copper complexes are by far more active than the free ligand ...
... phase of the cell cycle and eventually to cell death by apoptosis. They also indirectly demonstrated that the active species was the iron(II) complex of 1-formylisoquinoline thiosemicarbazone. In fact, it was later discovered that iron and copper complexes are by far more active than the free ligand ...
Auxin-Dependent Cell Division and Cell Elongation. 1
... of NAA are raised further. These data show that the optimum for the stimulation of cell division is situated at 10 mM when 2,4-D is used as exclusive auxin, whereas it is shifted by 1 order of magnitude when NAA is used instead. It is not clear, however, whether the intracellular concentrations of b ...
... of NAA are raised further. These data show that the optimum for the stimulation of cell division is situated at 10 mM when 2,4-D is used as exclusive auxin, whereas it is shifted by 1 order of magnitude when NAA is used instead. It is not clear, however, whether the intracellular concentrations of b ...
Endoplasmic reticulum stress-induced programmed cell death in
... in animal cells. In plants, PCD has been observed during tracheary elements differentiation (Kuriyama and Fukuda, 2002), aerenchyma formation (Gunawardena et al., 2001), hypersensitive response (HR) (Heath, 2000) and as a consequence of several biological and chemical stresses (Beers and McDowell, 2 ...
... in animal cells. In plants, PCD has been observed during tracheary elements differentiation (Kuriyama and Fukuda, 2002), aerenchyma formation (Gunawardena et al., 2001), hypersensitive response (HR) (Heath, 2000) and as a consequence of several biological and chemical stresses (Beers and McDowell, 2 ...
Page 07-LS1-1. Conduct an investigation to provide evidence that
... Examples of behaviors that affect the probability of animal reproduction could include nest building to protect young from cold, herding of animals to protect young from predators, and vocalization of animals and colorful plumage to attract mates for breeding. Examples of animal behaviors that affec ...
... Examples of behaviors that affect the probability of animal reproduction could include nest building to protect young from cold, herding of animals to protect young from predators, and vocalization of animals and colorful plumage to attract mates for breeding. Examples of animal behaviors that affec ...
Mast cells and dendritic cells form synapses that facilitate antigen
... As immune modulators, both MCs and DCs are capable of secreting a wide variety of cytokines to stimulate or repress immune responses. To determine whether direct cell–cell interactions might influence cellular responses, cytokine secretion was measured using ELISA. MCs and imDCs were co-incubated to ...
... As immune modulators, both MCs and DCs are capable of secreting a wide variety of cytokines to stimulate or repress immune responses. To determine whether direct cell–cell interactions might influence cellular responses, cytokine secretion was measured using ELISA. MCs and imDCs were co-incubated to ...
Positioning the Flagellum at the Center of a Dividing Cell To
... ahead) but pulls it in the other half of the population (cells that swim with the flagellated pole ahead) (Fig. S3A). In contrast, for SS-5 cells, the position of the flagellum relative to the swimming direction is the same in all cells but will depend on the oxygen concentration; in the Northern He ...
... ahead) but pulls it in the other half of the population (cells that swim with the flagellated pole ahead) (Fig. S3A). In contrast, for SS-5 cells, the position of the flagellum relative to the swimming direction is the same in all cells but will depend on the oxygen concentration; in the Northern He ...
Divergence between motoneurons: gene
... being differentially expressed. Significant differences in expression were observed for 1.8% (207/11,552) of the genes when comparing sympathetic preganglionic MNs with the medial motor column. Lateral and medial MNs showed the least divergence, with 1.3% (150/ 11,552) of the genes being differentia ...
... being differentially expressed. Significant differences in expression were observed for 1.8% (207/11,552) of the genes when comparing sympathetic preganglionic MNs with the medial motor column. Lateral and medial MNs showed the least divergence, with 1.3% (150/ 11,552) of the genes being differentia ...
New Views on the Plant Cytoskeleton
... actin microfilament arrays in specific cell functions. The shift to a more integrative approach in the last few years has revolutionized the way we look at the plant cytoskeleton. Our initial view of static images has shifted to dramatic motion pictures of live, dynamic networks, and descriptive vie ...
... actin microfilament arrays in specific cell functions. The shift to a more integrative approach in the last few years has revolutionized the way we look at the plant cytoskeleton. Our initial view of static images has shifted to dramatic motion pictures of live, dynamic networks, and descriptive vie ...
Promega Notes 99: NIH Chemical Genomics Center: Small
... Nuclear factor kappa-B (NF-κB) plays an important role in normal B cell development and survival. Diffuse large B cell lymphoma (DLBCL) is the most commonly observed type of non-Hodgkin’s lymphoma. Gene expression analysis has identified an activated B cell-like subtype of DLBCL (ABC-DLBCL) that exp ...
... Nuclear factor kappa-B (NF-κB) plays an important role in normal B cell development and survival. Diffuse large B cell lymphoma (DLBCL) is the most commonly observed type of non-Hodgkin’s lymphoma. Gene expression analysis has identified an activated B cell-like subtype of DLBCL (ABC-DLBCL) that exp ...
New and Old Hereditary Forms of Renal Cell Carcinoma (RCC)
... Renal cell carcinoma affects approximately 30,000 individuals per year in the United States and is responsible for close to 12,000 annual deaths. Kidney cancer, is a tumor comprising several histologic subtypes, the molecular hallmarks of which support their classification as distinct entities. Whi ...
... Renal cell carcinoma affects approximately 30,000 individuals per year in the United States and is responsible for close to 12,000 annual deaths. Kidney cancer, is a tumor comprising several histologic subtypes, the molecular hallmarks of which support their classification as distinct entities. Whi ...
A Comparative Analysis of Extra-Embryonic Endoderm Cell
... Prior to gastrulation in the mouse, all endodermal cells arise from the primitive endoderm of the blastocyst stage embryo. Primitive endoderm and its derivatives are generally referred to as extra-embryonic endoderm (ExEn) because the majority of these cells contribute to extra-embryonic lineages en ...
... Prior to gastrulation in the mouse, all endodermal cells arise from the primitive endoderm of the blastocyst stage embryo. Primitive endoderm and its derivatives are generally referred to as extra-embryonic endoderm (ExEn) because the majority of these cells contribute to extra-embryonic lineages en ...
Fission yeast Tor2 promotes cell growth and represses cell
... To determine the relevance of G1 arrest for the Tor2 inactivation cell response, we deleted rum1+ or ste9+ genes in the tor2 conditional strain h90 tor2-51 and analysed its phenotype at the restrictive temperature. As shown in Fig. 4A, in the absence of rum1+ or ste9+, h90 tor2-51 cells did not arre ...
... To determine the relevance of G1 arrest for the Tor2 inactivation cell response, we deleted rum1+ or ste9+ genes in the tor2 conditional strain h90 tor2-51 and analysed its phenotype at the restrictive temperature. As shown in Fig. 4A, in the absence of rum1+ or ste9+, h90 tor2-51 cells did not arre ...
Germinal Center Dynamics Revealed by Multiphoton Microscopy with a Photoactivatable Fluorescent Reporter
... of CD40 and BCR ligation (Basso et al., 2004; Zhu et al., 2004). This analysis showed the LZ population to be strongly imprinted with the signatures of both of these activation signals (Figure 3D and Table S3). Taken together, our cell-cycle and gene expression analyses support the notion that cells ...
... of CD40 and BCR ligation (Basso et al., 2004; Zhu et al., 2004). This analysis showed the LZ population to be strongly imprinted with the signatures of both of these activation signals (Figure 3D and Table S3). Taken together, our cell-cycle and gene expression analyses support the notion that cells ...
The Cytology of the Gram-positive Cocci
... It is of interest to observe that the parallel between smooth and rough variants in the bacillary genera, and short- and long-chained streptococci, appears to extend to their minute structure. In both groups, the variant which adheres in chains after division, and consequently forms ' Medusa-head' c ...
... It is of interest to observe that the parallel between smooth and rough variants in the bacillary genera, and short- and long-chained streptococci, appears to extend to their minute structure. In both groups, the variant which adheres in chains after division, and consequently forms ' Medusa-head' c ...
Chapter 7: Cellular Structure and Function - Bellbrook
... and sizes. You also might infer that cells differ based on the function they perform for the organism. If so, you are correct! However, all cells have at least one physical trait in common: they all have a structure called a plasma membrane. A plasma membrane, labeled in Figure 7.4, is a special bou ...
... and sizes. You also might infer that cells differ based on the function they perform for the organism. If so, you are correct! However, all cells have at least one physical trait in common: they all have a structure called a plasma membrane. A plasma membrane, labeled in Figure 7.4, is a special bou ...
Teratocarcinoma stem cells as a model for differentiation inthe
... embryonic development by directing, for instance, cell migration (e.g. Hay, 1981). Fibronectin has been suggested specifically to promote the differentiation and migration of parietal the endoderm cells (Grabel and Watts. 1987). On the other hand, differentiation of cells can influence their adheren ...
... embryonic development by directing, for instance, cell migration (e.g. Hay, 1981). Fibronectin has been suggested specifically to promote the differentiation and migration of parietal the endoderm cells (Grabel and Watts. 1987). On the other hand, differentiation of cells can influence their adheren ...
Cell cycle
The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.