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The endoplasmic reticulum exerts control over organelle streaming
The endoplasmic reticulum exerts control over organelle streaming

... cytoskeletal forces are in place to propel these organelles. The ER is arguably one of the organelles with the largest membrane extension and it is in close vicinity to the other organelles (Fig. S3). The evidence proposed above that ER streaming does not entirely depend on the cytoskeleton (Fig. 2C ...
CONTRIBUTION OF STEM CELLS AND DIFFERENTIATED CELLS
CONTRIBUTION OF STEM CELLS AND DIFFERENTIATED CELLS

... choice of lineage being determined by the specific location or microenvironment of the cell3,8 (FIG. 2b). Stem cells are not the only cells within the epidermis that are capable of proliferation. Stem-cell progeny that are destined to undergo terminal differentiation can divide a small number of tim ...
Systemic and cell type-specific gene expression patterns in scleroderma skin.
Systemic and cell type-specific gene expression patterns in scleroderma skin.

... culture. Most of the genes that are expressed at a higher level in normal skin relative to scleroderma skin are not easily associated with any single cell type represented in this data set (Fig. 2G). Immunoglobulins and CD53 genes associated with B lymphocytes (including plasma cells; Fig. 2C) show ...
Synthesis of a Callosic Substance during Rhizoid Differentiation in
Synthesis of a Callosic Substance during Rhizoid Differentiation in

... The presence of extracellular Ca2+ is indispensable for induction of rhizoid differentiation (Inoue et al. 2002). Cells died when EGTA was added in order to lower the external concentration of Ca2+. However, cells could survive in the presence of EGTA when the medium was also supplemented with MgCl2 ...
In vitro development of inner cell masses isolated immunosurgically
In vitro development of inner cell masses isolated immunosurgically

... unkilled trophectoderm cells persisted on the surface of every one of these inner cell masses. A second possibility, which we favour, is that for some time after differentiation of the morula into a blastocyst, some or all of the cells in the inner cell mass are able to reverse their fate and form t ...
Protein traffic in polarized epithelial cells: the polymeric
Protein traffic in polarized epithelial cells: the polymeric

... organ and an underlying tissue. For many decades physi­ ologists have studied the movements of small molecules such as water, ions or sugars across epithelia. It has become increasingly clear that large molecules, such as proteins, can also cross an epithelial layer. One way that this can occur is b ...
Tissue Engineering - Gateway Coalition
Tissue Engineering - Gateway Coalition

... To create an interconnected porous matrix suitable for bone tissue ingrowth, PLAGA will be put into solution and emulsified in PVA, a surface acting agent (surfactant). The surfactant forces the polymer solution into small spheres, the size of which depends on the concentration of polymer solution, ...
Hedgehog proteins: expression and function in the thymus
Hedgehog proteins: expression and function in the thymus

Phospholipid Bilayers
Phospholipid Bilayers

... The plasma membrane (also known as the cell membrane) forms a barrier between the cytoplasm inside the cell and the environment outside the cell. It protects and supports the cell and also controls everything that enters and leaves the cell. It allows only certain substances to pass through, while k ...
MALE GENITAL DERMATOLGY
MALE GENITAL DERMATOLGY

... • Symptoms not a discriminating feature in genital dermatology • Only a limited number of symptoms in adult anogenital region: • Itch • Pain ...
Bridging the divide between cytokinesis and cell
Bridging the divide between cytokinesis and cell

... trans-Golgi network (TGN) to plasma membrane (1) contributes to anisotropic cell expansion and has been thought to be the main pathway of membrane delivery to the cell plate on the basis of EM studies. Although uncharacterized in plants, a Golgi to endosome secretion pathway (5) cannot be ruled out. ...
Q5D - ICH
Q5D - ICH

... procedures that allow the cell bank containers to be traced. This should include a description of the documentation system as well as that of a labelling system which can withstand the process of preservation, storage, and recovery from storage without loss of labelling information on the container. ...
3-4 Teacher
3-4 Teacher

... Transforming Animal Cells Many egg cells are large enough that DNA can be directly injected into the nucleus. ...


... Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been fully determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies sugg ...
PROCARYOTIC AND EUCARYOTIC CELLS
PROCARYOTIC AND EUCARYOTIC CELLS

... Know that PCR (polymerase chain reaction) can start with one double helix and after 20 replications (DNA replications) forms 1,000,000 double helices. ...
Growth Control: A Saga of Cell Walls, ROS, and
Growth Control: A Saga of Cell Walls, ROS, and

... signaling networks, little is known on how these networks are integrated with the cell expansion machinery. Members of the CrRLK1L family control cell wall properties and cell expansion in a variety of developmental and environmental contexts. Two recent reports provide exciting new insights into th ...
The enhancement of histone H4 and H2A serine 1 phosphorylation
The enhancement of histone H4 and H2A serine 1 phosphorylation

... cassette’ in which specific combinations of histone modifications may elicit distinct biological functions (Fischle et al. 2003b). For example, in addition to serine 1, the arginine 3 and lysine 5 residues of H4 are known to be methylated and acetylated, respectively, in association with different n ...
Antiproliferative and apoptotic effects of O
Antiproliferative and apoptotic effects of O

... mitotic activity to the same extent as TRX, thus there is a discrepancy between the slight reduction in DNA synthesis and a large decrease in mitotic index after DFO treatment. In addition, we found that TRX induced accumulation of cells in the G1 and G2 phases of the cell cycle whereas DFO arrested ...
PDF
PDF

... embryos were removed during the first cell cycle, prior to teloplasm formation. The vitelline membrane was first weakened by a 6-min incubation at room temperature in 0.3% bacterial protease (type XIV, Sigma; Astrow et al, 1989). The embryos were washed in HL medium and their vitelline membranes wer ...
Single Cell Electrical Characterization Techniques
Single Cell Electrical Characterization Techniques

... 20 years to develop the experimental tools and technologies able to carry out single cell measurement. In addition, in depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis [16]. In conventional methods of cellular an ...
Barbara McClintock - Nobel Lecture
Barbara McClintock - Nobel Lecture

... In the mid-nineteen-thirties another event inducing chromosome rupture was discovered. It revealed why crossing-over should be suppressed between the centromere and the nucleolus organizer in organisms in which chiasmata terminalize, from the initial location of a crossover to the end of the arm of ...
The Role of Mycoplasma Membrane Proteins in the
The Role of Mycoplasma Membrane Proteins in the

... These results support the suggestion of Manchee & Taylor-Robinson (1969) that protein receptors in the membrane of Mycoplasma hominis are involved in the adsorption of HHeLa cells. Nevertheless, the proteins extracted with urea or phenol did not absorb all of the adsorption-inhibiting antibody from ...
Anatomy & physiology of cells
Anatomy & physiology of cells

... The cell is the basic unit of structure and function in living things. Cells vary in their shape size, and arrangements but all cells have similar components, each with a particular function. Some of the 100 trillion of cells make up human body. All human cell are microscopic in size, shape and func ...
Mechanisms and cellular roles of local protein synthesis in mammalian cells
Mechanisms and cellular roles of local protein synthesis in mammalian cells

... specific mRNA transport pathways by studying them. Despite this, it is abundantly clear that synaptic activity influences the distribution and motility of these entities, and based on this it has been proposed that mRNA localizes to active synapses through the RNA-binding factors and activities asso ...
Fluorescent tagging of an endogenous gene by
Fluorescent tagging of an endogenous gene by

... Here we have demonstrated that fluorescent tagging of an endogenous protein can be accomplished with the Edit-R CRISPR-Cas9 genome engineering platform and a donor plasmid template. We inserted TurboGFP in-frame with the endogenous SEC61B gene and selected individual clonal cell lines, which were ve ...
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Cell cycle



The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.
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