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Note. The efficiency of Benzonase treatment can be determined by
Note. The efficiency of Benzonase treatment can be determined by

... 1. Warm 293T culture medium to 37⁰C. 2. Gently remove transfection medium from cells 3. Very slowly add fresh culture medium to cells. Use 15-25 ml medium for a 150 mm culture dish. a. Note: cells will become dislodged very easily at this stage, so use extreme caution b. Note: if working with sever ...
Chapter 10 – The cell is the basic unit of life. Using a Microscope
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Cells—The Building Blocks of Life
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... function during development. • Cells vary in size, shape, and internal components • The variety of cell shapes include round, flat, long, rectangle, etc. • Some cells have different extensions from the cell membrane ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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