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Tools of the Biologist
Tools of the Biologist

... Tools of the Biologist 6.) Electron Microscope Focus beams of electrons on specimens 1000x smaller than can be viewed with Light microscope ...
Medical Anatomy Final Review
Medical Anatomy Final Review

... a. energy source b. sugars • Lipids: fats a. higher source of energy; harder to break • Proteins: made of amino acids a. body tissues b. enzymes/hormones c. held together by peptide bonds • Nucleic Acids: DNA/RNA a. adenine/thymine b. cytosine/guanine 02.12 ATP and Energy Conversion • Adenosine Trip ...
Maintaining a Dynamic Equilibrium The Need for Homeostasis
Maintaining a Dynamic Equilibrium The Need for Homeostasis

... Organisms live in a world of changing conditions. But, to remain alive, every organism needs to keep the conditions inside of itself fairly constant. An organism must have ways to keep its internal conditions from changing as its external environment changes. This ability of all living things to det ...
Seminar Paper - LENS - The University of Auckland
Seminar Paper - LENS - The University of Auckland

... The  method  decided  on  was  to  partially  DOWN  REGULATE  the  ABP1  protein  so  that  there  was  less  ABP1  than  normal, but there was some being produced.  This was achieved both in cell tissue culture and whole plants using  tobacco plants and Arabidopsis (a small fast growing plant that  ...
Pegvisomant, a Growth Hormone-Specific Antagonist, Undergoes
Pegvisomant, a Growth Hormone-Specific Antagonist, Undergoes

... residue G120 in binding site 2 of GH has been shown to inhibit GH-induced signal transduction (3–5). The genetically engineered GH receptor antagonist, B2036, is mutated at GH binding site 2 with G120K. B2036 has eight additional mutations at site 1 that increase the affinity of B2036 for soluble GH ...
specific role of lymphatic marker podoplanin in retinal pigment
specific role of lymphatic marker podoplanin in retinal pigment

... notion is a previous report showing that podoplanin facilitates lymphatic endothelial cell adhesion to the extracellular matrix (2). Though it is yet to be determined, it is plausible to speculate that podoplanin has a pro-adhesive effect among different cell types. Secondly, the integrity of the RP ...
Biology Unit 3 - Exchange of Materials
Biology Unit 3 - Exchange of Materials

... this water evaporates, water is pulled up through the xylem to take its place. This constant moving of water through the xylem from the roots to the leaves is known as the transpiration stream. Water moves into the roots from the soil by osmosis. It replaces the water constantly moving up the stem. ...
PiXL AQA – Exchange of Materials Powerpoint
PiXL AQA – Exchange of Materials Powerpoint

... Transpiration is the loss of water vapour from the surface of plant leaves through the stomata. Water evaporates from the surface of the leaves, through the open stomata. As this water evaporates, water is pulled up through the xylem to take its place. This constant moving of water through the xylem ...
3.5 Unit 3: Biology 3 B3.1.1 Dissolved Substances
3.5 Unit 3: Biology 3 B3.1.1 Dissolved Substances

... Transpiration is the loss of water vapour from the surface of plant leaves through the stomata. Water evaporates from the surface of the leaves, through the open stomata. As this water evaporates, water is pulled up through the xylem to take its place. This constant moving of water through the xylem ...
The Type I Membrane Protein EFF-1 Is Essential for Developmental
The Type I Membrane Protein EFF-1 Is Essential for Developmental

... Much less is known about the mechanisms that mediate plasma membrane fusion in fertilization and syncytium formation (Shemer and Podbilewicz, 2000). Fertilins and meltrins, members of the ADAM family of membrane glycoproteins, are no longer considered candidate fusogens (Cho et al., 2000; Hernandez ...
Fungi - Ms. Marcos` Biology Wiki
Fungi - Ms. Marcos` Biology Wiki

... secrete digestive enzymes  feed by absorption ...
7-3 Cell Boundaries - Eastern Wayne High
7-3 Cell Boundaries - Eastern Wayne High

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Cytokinesis Cytokinesis Cytokinesis Cytokinesis

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A Mammalian Cell Cycle Checkpoint Pathway - Wafik S. El
A Mammalian Cell Cycle Checkpoint Pathway - Wafik S. El

... group of every cell line utilized has not been identified, the lymphoblast line, 718 (Figures 2A and 2B), is complementation group A (the most common group). Two lymphoblast lines from AT patients classified as complementation group C (CSA and BMA) exhibited only about 2-fold increases in ~53 protei ...
Immunocytochemistry of Rhamnogalacturonan II in Cell Walls of
Immunocytochemistry of Rhamnogalacturonan II in Cell Walls of

... walls of all the cells in radish and rice roots, cultured tobacco cells, red clover root nodules, and lily growing pollen tubes. The label was denser in proximal to plasma membrane, and not detected in middle lamella, suggesting that borate may cross-link newly secreted pectic polysaccharides at the ...
Prokaryotic Cell Architecture(bacteria) Structurally, a bacterial cell
Prokaryotic Cell Architecture(bacteria) Structurally, a bacterial cell

... (e.g. a lake or stream) where the concentration of the business molecules of life is greater inside of the cell than in the environment. Hence, the bacterial cells must transport their nutrients from the environment and maintain a higher concentration of solutes inside the cell than outside the cell ...
Introduction to Virology I Viruses Defined
Introduction to Virology I Viruses Defined

... be read by host ribosomes. Literally, all viruses are parasites of their host cells’ mRNA translation system. The Baltimore classification system integrates this principle to construct an elegant molecular algorithm for virologists (Fig. 15). When the bewildering array of viruses is classified by th ...
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11 Diffuse Lymphatic Tissue

... cytoplasm shows variable degrees of basophilia and lacks specific granules. The lymphocyte population customarily is divided into small, medium, and large lymphocytes on the basis of their size, nuclear morphology, and intensity of cytoplasmic staining. Although useful for purposes of description, s ...
Gastrulation in C. elegans
Gastrulation in C. elegans

... phenomena, including morphogenesis, cell polarization, cell-cell signaling, actomyosin contraction and cell-cell adhesion. One distinct advantage of studying these phenomena in C. elegans is that genetic tools can be combined with high resolution live cell imaging and direct manipulations of the cel ...
Isolation and Quantitation of HIV in Peripheral
Isolation and Quantitation of HIV in Peripheral

... A characteristic feature of HIV infection in vitro is its ability to induce total or partial depletion of CD4+ target cells. Two related but distinct mechanisms have been correlated with HIV-induced cytopathicity: cell fusion, which results in the formation of multinucleated giant cells (syncytia), ...
Chlamydiacae - WordPress.com
Chlamydiacae - WordPress.com

... -After 24-48 hrs ,EBs are released and initiate a new cycle of infection -The mass of EBs → Inclusion body→detected by histologic stains ...
cell transport
cell transport

... 5.15 Transport proteins may facilitate diffusion across membranes • In facilitated diffusion – Transport proteins that span the membrane bilayer help substances diffuse down a concentration gradient • To transport the substance, a transport protein may ...
351 CHAPTER 21 Gram-Positive Cell Wall
351 CHAPTER 21 Gram-Positive Cell Wall

... residue bears a tetrapeptide of alternating l- and d-amino acids. Adjacent glycan chains are cross-linked into sheets by peptide bonds between the third amino acid of one tetrapeptide and the terminal d-alanine of another. The same cross-links between other tetrapeptides connect the sheets to form a ...
Mechanics of epithelial tissue formation in early insect embryos
Mechanics of epithelial tissue formation in early insect embryos

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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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