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Patterns in nature
Patterns in nature

... from non–living matter such as piles of rubbish and organisms such as rats and flies were produced by rotting meat. Francesco Redi (1626–1697) showed that maggots only arose from meat that flies had visited and Louis Pasteur (1822–1888) showed that micro–organisms only come from other micro–organism ...
Lecture 21 Retroviral Vector Method To increase the probability of
Lecture 21 Retroviral Vector Method To increase the probability of

... Lecture 21 Retroviral Vector Method To increase the probability of expression, gene transfer is mediated by means of a carrier or vector, generally a virus or a plasmid. Retroviruses are commonly used as vectors to transfer genetic material into the cell, taking advantage of their ability to infect ...
CONNECTIVE TISSUE I
CONNECTIVE TISSUE I

... Synthesized by a wide variety of cells, including fibroblasts, chrondroblasts, osteoblasts, smooth muscle, endothelial and epithelial cells. It is the most abundant protein in the body. Synthesis (intracellular and extracellular components): In the RER, polypeptide chains known as procollagen are fo ...
pteridophyta - Institutul de Biologie
pteridophyta - Institutul de Biologie

... collected from the swampy area near the Comana village from which explants of somatic tissues of different origins have been taken. Nodal areas on the rhizome and the branching of the leaves presented a high reactivity. The plant prefers a semi liquid culture medium, poor in mineral salts such as Kn ...
Microelectrode techniques in plant cells and microorganisms
Microelectrode techniques in plant cells and microorganisms

... uncertainty awaits resolution. Enzymatically produced protoplasts also lack the plasma membrane/extracellular matrix connections as a result of wall removal. The cell wall performs many functions which may have significant bearing on the physiology of the cell. For example, plasma membrane-wall conn ...
Ch 18 - protists
Ch 18 - protists

... The food vacuoles will fuse with LYSOSOMES (sacks of digestive enzymes) and be digested. ...
Abnormal Cellular Growth and Development
Abnormal Cellular Growth and Development

... can get interrupted, as in this case…lack of iodine causes thyroid to increase in size (hypertrophy and hyperplasia) ...
Negative Controls of Cell Proliferation: Human
Negative Controls of Cell Proliferation: Human

... is sketchy and full of controversy. Several hypotheses have been proposed and they vary significantly depending on the premises on which they are based. For example, while hypotheses on positively regulated mechanisms rely on the notion that cells are waiting for a signal to trigger their entering t ...
Class Notes
Class Notes

... ○ This lock-and-key recognition ensures that eggs will be fertilized only by sperm of the same species. The sperm and egg plasma membranes fuse, and the sperm nucleus enters the egg’s cytoplasm. In response to contact or fusion of the membranes, Na+ channels in the egg’s plasma membrane ...
the bacterial cell - Journal of Clinical Pathology
the bacterial cell - Journal of Clinical Pathology

... fimbriation is enhanced by frequent passage in broth culture but has no relationship to motility. These fimbriae have strong adhesive properties and are responsible for agglutination of the red cells of various animal species. The fimbriae from different serological types of Shigella flexneri are an ...
chapter 47 - Biolympiads
chapter 47 - Biolympiads

... ○ This lock-and-key recognition ensures that eggs will be fertilized only by sperm of the same species. The sperm and egg plasma membranes fuse, and the sperm nucleus enters the egg’s cytoplasm. In response to contact or fusion of the membranes, Na+ channels in the egg’s plasma membrane ...
Cork and Onion Cells
Cork and Onion Cells

... Over 300 years ago, Robert Hooke looked at cork under a microscope. He saw little boxlike structures as pictured above. He called these structures cells. Cork does not contain living tissue and only contains a cell wall. By the 19th century, it was accepted that all living things are made of cells. ...
Reovirus for the treatment of acute myeloid leukaemia
Reovirus for the treatment of acute myeloid leukaemia

... fibroblasts increased expression of both CD54 and CD55 was observed. The efficacy of Cavatak virotherapy was increased after co-culture with CD40L positive cells. CLL cell lines are susceptible to Cavatak virotherapy but human primary cells were resistant. In our model of lymph node resistant diseas ...
The DREAM Complex Mediates GIST Cell Quiescence and Is a
The DREAM Complex Mediates GIST Cell Quiescence and Is a

... Here, we show that 1.Imatinib induces GIST cell quiescence in vivo and in vitro. 2. This process involves the DREAM complex as evidenced by upregulation of p130, increased p130/E2F4/LIN37 complex formation, and enhanced phosphorylation of the DREAM subunit LIN52. 3. Importantly, inhibition of DREAM ...
Topic 7
Topic 7

... A fibroblast is a type of cell that synthesizes the extracellular matrix and collagen, the structural framework for animal tissues, and plays a critical role in wound healing. Fibroblasts are the most common cells of connective tissue in animals. ...
Theoretical background
Theoretical background

... growing with a generation time of 85 min [6] . The C period shows the duration of the DNA replication cycle. The D period is the time required between the termination of DNA replication and the separation into two daughter cells. The Z period shows the duration of the period in which FtsZ can be det ...
File
File

... oligodendrocyte forms myelin sheaths for several (3–50) nerve fibers. The axolemma shows a thickening where the cell membrane of the oligodendrocyte comes into contact with it. This limits the diffusion of materials into the periaxonal space between the axon and the myelin sheath ...
Sp100 is important for the stimulatory effect of
Sp100 is important for the stimulatory effect of

... Sp100 and HIPK2 cooperate to induce p53-dependent gene expression To investigate the impact of Sp100 on HIPK2-induced p53 activity, human U2OS cells were transfected with various combinations of expression vectors encoding Sp100 and the wild-type and kinase inactive (K221A) point mutant of HIPK2, al ...
The Biology Staff Handbook
The Biology Staff Handbook

... The number of slugs found under discarded bricks were: 5,9,9,8,6,9,3,6 Arrange the data in order: 3,5,6,6,8,9,9,9 The median is the middle number, or the mean of the middle two numbers. The median number of slugs per brick = 6 + 8 = 7 slugs per brick ...
comaneanu r - Revista de Chimie
comaneanu r - Revista de Chimie

... The term of biomaterial [1] was for the first time used in a conference organized by Clemson University, USA, when it was defined as „an inert substance from the systemic and pharmaceutical point of view, intended for implants or embedded in living systems.” Another definition of the biomaterial ter ...
Unit 2 summary notes
Unit 2 summary notes

... The number of slugs found under discarded bricks were: 5,9,9,8,6,9,3,6 Arrange the data in order: 3,5,6,6,8,9,9,9 The median is the middle number, or the mean of the middle two numbers. The median number of slugs per brick = 6 + 8 = 7 slugs per brick ...
FADDdel-GFP Modified Mouse Insulinoma Cells Counteract the
FADDdel-GFP Modified Mouse Insulinoma Cells Counteract the

... Hind III and Not I. Transfection, screening and detection of FADDdel-GFP in NIT NIT (mouse insulinoma cells) were presented by Australian WEH1 laboratory. NIT were passaged on 24well tissue culture plates full of Dulbecco’s Modified Eagale’s Medium (DMEM, Sigma) supplemented with 10% fetal calf seru ...
3.3 Cell Membrane Cell membranes are composed of two
3.3 Cell Membrane Cell membranes are composed of two

... Diffusion and osmosis are types of passive transport. • There are three types of solutions. • isotonic ...
Technical Information Sheet No.15
Technical Information Sheet No.15

... composition of medium. In order not to neglect any typical morphological changes arising during the growth cycle, it is also important to examine cultures of different age up to several days. 5. To use only purified agar brands (such as Oxoid Agar No.1, Difco Bacto Agar, and others) since normal aga ...
Tools of the Biologist
Tools of the Biologist

... Tools of the Biologist 6.) Electron Microscope Focus beams of electrons on specimens 1000x smaller than can be viewed with Light microscope ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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