Histology Lab Manual - Histology Laboratory Manual
... 1. Understand and be able to describe how the most common dye combination, hematoxylin and eosin (H&E), stains various components of cells and tissues. 2. Identify cells and subcellular organelles. 3. Relate the appearance of a cell as seen with the light microscope (LM) with that at with the electr ...
... 1. Understand and be able to describe how the most common dye combination, hematoxylin and eosin (H&E), stains various components of cells and tissues. 2. Identify cells and subcellular organelles. 3. Relate the appearance of a cell as seen with the light microscope (LM) with that at with the electr ...
Connective tissue
... Provide support in blood vessel walls. Form a network around fat cells, nerve fibers, and skeletal and smooth muscle cells. Provide support and strength and form the stroma (supporting framework) of many soft organs (spleen, lymph nodes). Help form basement ...
... Provide support in blood vessel walls. Form a network around fat cells, nerve fibers, and skeletal and smooth muscle cells. Provide support and strength and form the stroma (supporting framework) of many soft organs (spleen, lymph nodes). Help form basement ...
441_2009_904_MOESM1_ESM - Springer Static Content Server
... population included cells with a thick dendritic tuft (ET-like cells). In our analysis, we assumed that all cells in the GL are stained by at least one of our antibodies (see Fig. 6). Combinations of double and triple stainings were used to establish IFs. We could base our classification of IFs on c ...
... population included cells with a thick dendritic tuft (ET-like cells). In our analysis, we assumed that all cells in the GL are stained by at least one of our antibodies (see Fig. 6). Combinations of double and triple stainings were used to establish IFs. We could base our classification of IFs on c ...
Tissues Lecture
... free edge than at the basement, which is the opposite of most epithelium. The cells shape transitions depending on whether a structure, like the bladder, is contracted or distended. Special Features: Epithelial tissue is often specialized for various functions on the apical surface of the tissue. Th ...
... free edge than at the basement, which is the opposite of most epithelium. The cells shape transitions depending on whether a structure, like the bladder, is contracted or distended. Special Features: Epithelial tissue is often specialized for various functions on the apical surface of the tissue. Th ...
Figure S1.
... Day 3 4X: The wound has extended deeper with excess fibrin. The muscle layer is also exposed. The crush artifact on the surface is evident. The wound exhibits excessive infiltrates of mononuclear and polymorphonuclear cells, the presence of necrotic tissue debris, and a hypodermis expanded by edema. ...
... Day 3 4X: The wound has extended deeper with excess fibrin. The muscle layer is also exposed. The crush artifact on the surface is evident. The wound exhibits excessive infiltrates of mononuclear and polymorphonuclear cells, the presence of necrotic tissue debris, and a hypodermis expanded by edema. ...
File
... Function: Allows materials to pass by diffusion and filtration in sites where protection is not important; secretes lubricating substances in ...
... Function: Allows materials to pass by diffusion and filtration in sites where protection is not important; secretes lubricating substances in ...
Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial
... PA103DU-infected monolayers led us to test whether one of the type III-secreted proteins elaborated by PA103DU, and absent in PA103pscJ::Tn5, could mediate cell rounding and inhibition of wound closure. ExoT, a Rhofamily GAP (Krall et al., 2000) that causes cell rounding of HeLa and Chinese Hamster ...
... PA103DU-infected monolayers led us to test whether one of the type III-secreted proteins elaborated by PA103DU, and absent in PA103pscJ::Tn5, could mediate cell rounding and inhibition of wound closure. ExoT, a Rhofamily GAP (Krall et al., 2000) that causes cell rounding of HeLa and Chinese Hamster ...
Sample pages 1 PDF
... is made from paraformaldehyde (PFA) powder and used immediately. Glutaraldehyde was introduced by Sabatini et al. in 1963 as an especially useful fixative for ultrastructural studies [22]. This compound has two aldehyde groups separated by three methylene bridges. Glutaraldehyde is a much more effic ...
... is made from paraformaldehyde (PFA) powder and used immediately. Glutaraldehyde was introduced by Sabatini et al. in 1963 as an especially useful fixative for ultrastructural studies [22]. This compound has two aldehyde groups separated by three methylene bridges. Glutaraldehyde is a much more effic ...
Microbiology – Chapter 4, Bacteria
... Some antibiotics work by messing with the “ribosome” Slightly smaller than our eukaryote ribosome, so antibiotic can work ...
... Some antibiotics work by messing with the “ribosome” Slightly smaller than our eukaryote ribosome, so antibiotic can work ...
HISTOLOGY LAB
... Columnar Epithelium this tissue is not found lining villi. The presence of cilia is an important identification mark as this is the only ciliated tissue you will examine this quarter. Description: Looks like a stratified tissue, but its not. Each cell extends from the basement membrane to the lumen. ...
... Columnar Epithelium this tissue is not found lining villi. The presence of cilia is an important identification mark as this is the only ciliated tissue you will examine this quarter. Description: Looks like a stratified tissue, but its not. Each cell extends from the basement membrane to the lumen. ...
İneklerde Furstenberg Rozeti Üzerinde Histolojik ve
... Mammary gland tissue samples were obtained from Çubuk slaughterhouse. California mastitis test (CMT) was applied by taking the milk samples from 10 Holstein cows whose general condition are well for the outside appearance, have not got anatomical defects in their mammary gland and not showing inflam ...
... Mammary gland tissue samples were obtained from Çubuk slaughterhouse. California mastitis test (CMT) was applied by taking the milk samples from 10 Holstein cows whose general condition are well for the outside appearance, have not got anatomical defects in their mammary gland and not showing inflam ...
Classification of Bacteria
... Ex. – Campylobacter jejuni ◦ Symptom – tenesmus: the sensation of desire to defecate, which is common and occurs frequently , with out the production of significant amounts of feces (often small amounts of ...
... Ex. – Campylobacter jejuni ◦ Symptom – tenesmus: the sensation of desire to defecate, which is common and occurs frequently , with out the production of significant amounts of feces (often small amounts of ...
Localization of the mei-1 Gene Product of
... ELL division necessitates the coordinate expression of the gene products required for the proper localization and organization of the spindle (McIntosh and Koonce, 1989; Kuriyama and Nislow, 1992; Rose et al., 1993; Strome, 1993). While tubulin forms the backbone of the structure, numerous accessory ...
... ELL division necessitates the coordinate expression of the gene products required for the proper localization and organization of the spindle (McIntosh and Koonce, 1989; Kuriyama and Nislow, 1992; Rose et al., 1993; Strome, 1993). While tubulin forms the backbone of the structure, numerous accessory ...
Bacteria viability assessment after photocatalytic treatment Yanling Cai Maria Strømme
... green fluorescence emission and stains both live and dead bacterial DNA, and propidium iodide, which has a red fluorescence emission and penetrates only damaged cell membranes. When the fluorescence is measured directly (e.g., with a microplate reader) or combined with flow cytometery, bacterial via ...
... green fluorescence emission and stains both live and dead bacterial DNA, and propidium iodide, which has a red fluorescence emission and penetrates only damaged cell membranes. When the fluorescence is measured directly (e.g., with a microplate reader) or combined with flow cytometery, bacterial via ...
Anatomy Step I Basic Histology Slides (Repariert)
... -2 ways of ossification: -intramembranous oss.: direct mineralization of matrix secreted by osteoblasts -chondrogenic oss.: deposition of bone matrix an a preexisting cartilage matrix -> this takes place in a piece of hyaline cartilage -responsible for formation of long and short bones -steps: 1) fo ...
... -2 ways of ossification: -intramembranous oss.: direct mineralization of matrix secreted by osteoblasts -chondrogenic oss.: deposition of bone matrix an a preexisting cartilage matrix -> this takes place in a piece of hyaline cartilage -responsible for formation of long and short bones -steps: 1) fo ...
SDS PAGE analysis of proteins
... 1. Wash the glass plates well with soap and water, rinse thoroughly with distilled water and finally wash with 95% EtOH. Let air dry. 2. Lay the spacers down along both side of the bigger glass plate. Then place the other one on the top. Clamp plates together, making sure that the spacers and plates ...
... 1. Wash the glass plates well with soap and water, rinse thoroughly with distilled water and finally wash with 95% EtOH. Let air dry. 2. Lay the spacers down along both side of the bigger glass plate. Then place the other one on the top. Clamp plates together, making sure that the spacers and plates ...
Influence of salinity on the localization of Na+/K+
... Different superscript letters indicate that the means are significantly different from one another (P<0.05, Student–Newman–Keuls test). ...
... Different superscript letters indicate that the means are significantly different from one another (P<0.05, Student–Newman–Keuls test). ...
PDF
... When stained with Delafield's haematoxylin and eosin the whole of this preotolithic material took up the pink eosin stain. With PAS, however, the protein precipitate was negative and took up only the counterstain, light green, whereas the membranous specks were PAS-positive and stained a pink to mau ...
... When stained with Delafield's haematoxylin and eosin the whole of this preotolithic material took up the pink eosin stain. With PAS, however, the protein precipitate was negative and took up only the counterstain, light green, whereas the membranous specks were PAS-positive and stained a pink to mau ...
Chapter 3 Cells and Tissues
... – Cuboidal – cube-shaped (as tall as they are wide), like dice – Columnar – column-like (tall & column shaped) *In each, the shape of the nucleus conforms to that of the cell. Thus, nuclear shape can be very helpful when attempting to distinguish epithelial types ...
... – Cuboidal – cube-shaped (as tall as they are wide), like dice – Columnar – column-like (tall & column shaped) *In each, the shape of the nucleus conforms to that of the cell. Thus, nuclear shape can be very helpful when attempting to distinguish epithelial types ...
Connective tissue
... skeleton (stroma) that supports other cell types including white blood cells, mast cells, and macrophages. ...
... skeleton (stroma) that supports other cell types including white blood cells, mast cells, and macrophages. ...
Function - My CCSD
... • Relationship btw/ ducts & glandular areas branched – several secretory areas share a common duct Always refers to glandular areas not to duct ...
... • Relationship btw/ ducts & glandular areas branched – several secretory areas share a common duct Always refers to glandular areas not to duct ...
Abundant protein-containing particles in the sea
... electron microscopy of samples sedimented by ultracentrifugation. Alldredge et al. (1993) discovered a class of transparent exopolymer particles (TEP) which could be visualized by staining with Alcian Blue (a dye that stains acidic mucopolysaccharides), apparently derived from phytoplankton (particu ...
... electron microscopy of samples sedimented by ultracentrifugation. Alldredge et al. (1993) discovered a class of transparent exopolymer particles (TEP) which could be visualized by staining with Alcian Blue (a dye that stains acidic mucopolysaccharides), apparently derived from phytoplankton (particu ...
recommendations for tissue preparation and formalin or
... cross-linking fixatives and usually destroy the integrity of cell organelles. On the other hand, they penetrate tissues rapidly, initiating the fixation process faster than formaldehyde fixatives. Also, because they destroy cell membranes, they permeablize cells at the same time they fix the cel ...
... cross-linking fixatives and usually destroy the integrity of cell organelles. On the other hand, they penetrate tissues rapidly, initiating the fixation process faster than formaldehyde fixatives. Also, because they destroy cell membranes, they permeablize cells at the same time they fix the cel ...
In situ hybridization
... with our samples, we will detect the mRNA by adding the substrate BCIP (bromo-chloro-indolyl-phosphate, also called X-phos – think of the parallel with X-Gal, bromo-chloro-indolyl-galactoside) and NBT (nitro blue tetrazolium). The cleavage of BCIP in the presence of NBT leads to a blue precipitate w ...
... with our samples, we will detect the mRNA by adding the substrate BCIP (bromo-chloro-indolyl-phosphate, also called X-phos – think of the parallel with X-Gal, bromo-chloro-indolyl-galactoside) and NBT (nitro blue tetrazolium). The cleavage of BCIP in the presence of NBT leads to a blue precipitate w ...
A Flow Cytometry Approach to Detect In vivo Chromatin Compaction
... development and pregnancy [15]. ...
... development and pregnancy [15]. ...
Staining
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.