Light and Electron Microscopy of the Sheath of a Blue
... given by Fritsch (1945)and Smith (1950). Cultivation. Cultures were maintained in Knop medium (Pringsheim, I949) and the medium given by Stanier, Doudoroff & Adelberg (1963,table 19-6) without a nitrogen source (referred to as medium A) or with NHJl (medium B). Bulk cultures were prepared in I 1. vo ...
... given by Fritsch (1945)and Smith (1950). Cultivation. Cultures were maintained in Knop medium (Pringsheim, I949) and the medium given by Stanier, Doudoroff & Adelberg (1963,table 19-6) without a nitrogen source (referred to as medium A) or with NHJl (medium B). Bulk cultures were prepared in I 1. vo ...
Improving Detection of Canine Distemper Virus in Formalin
... Heat-induced Antigen Retrieval (HIAR) In this study, through a combination of proteinase K digestion and autoclaving in a Trilogy solution, we found a relatively simple method that demonstrates an optimal ISH signal enhancement of CDV RNA. Formaldehyde, as a 10% neutral buffered formalin, is the mos ...
... Heat-induced Antigen Retrieval (HIAR) In this study, through a combination of proteinase K digestion and autoclaving in a Trilogy solution, we found a relatively simple method that demonstrates an optimal ISH signal enhancement of CDV RNA. Formaldehyde, as a 10% neutral buffered formalin, is the mos ...
Morphologic and functional characterization of granulocytes and
... smear). (C) Detail of mature eosinophils, illustrating characteristic pink cytoplasm and peripheral nucleus (May-Grünwald-Giemsa–stained blood smear). (D) Hematoxylin and eosin section of posterior kidney showing an interstitial niche of myelopoiesis lying between renal tubules (t) and blood vessel ...
... smear). (C) Detail of mature eosinophils, illustrating characteristic pink cytoplasm and peripheral nucleus (May-Grünwald-Giemsa–stained blood smear). (D) Hematoxylin and eosin section of posterior kidney showing an interstitial niche of myelopoiesis lying between renal tubules (t) and blood vessel ...
Localization of proteins involved in endocytosis at tubulobulbar
... spermatids at the time of sperm release. The main functions of the TBCs are to remove excess spermatid cytoplasm and acrosomal contents, internalize and recycle junctional complexes by endocytosis prior to spermiation. However, in addition to recycling some of the molecules undergo lysosomal degrada ...
... spermatids at the time of sperm release. The main functions of the TBCs are to remove excess spermatid cytoplasm and acrosomal contents, internalize and recycle junctional complexes by endocytosis prior to spermiation. However, in addition to recycling some of the molecules undergo lysosomal degrada ...
Low and High Nucleic Acid Content Bacteria: Reality or
... Activity of LNA and HNA cells: Several authors have described the occurrence of LNA and HNA clusters in different way. Gasol and co-workers (1995) found a relation between size and activity of bacteria in aquatic environment and demonstrated on the basis of CTC (5-cyano-2,3-ditolyl tetrazolium chlor ...
... Activity of LNA and HNA cells: Several authors have described the occurrence of LNA and HNA clusters in different way. Gasol and co-workers (1995) found a relation between size and activity of bacteria in aquatic environment and demonstrated on the basis of CTC (5-cyano-2,3-ditolyl tetrazolium chlor ...
Bovine Epididymal Sperm Morphology Obtained from
... PD, respectively. The proportion of live spermatozoa from caudal epididymis was 86.1%, of which 9.9% were NPD, 68.3 and 7.9% had DD and PD, respectively. Of dead spermatozoa, 10.1% had no droplet and 3.3 and 0.5% had DD and PD, respectively. No significant difference observed between different parts ...
... PD, respectively. The proportion of live spermatozoa from caudal epididymis was 86.1%, of which 9.9% were NPD, 68.3 and 7.9% had DD and PD, respectively. Of dead spermatozoa, 10.1% had no droplet and 3.3 and 0.5% had DD and PD, respectively. No significant difference observed between different parts ...
I. READING (10 marks) Read the following passage and answer the
... V. WRITING: (10 marks) A. Look at the pictures given below. They describe how to remove an ink stain. Number the steps in time order. (2.5 marks) ...
... V. WRITING: (10 marks) A. Look at the pictures given below. They describe how to remove an ink stain. Number the steps in time order. (2.5 marks) ...
semi-automatic segmentation, detection and classification of gram
... can make the analysis at night it results in that some samples are not analyzed until the morning. An automatic system could provide a microscopic analysis both day and night. After the bacteria sample has been cultivated it has to be stained to make the bacteria visible in a light microscopy. The m ...
... can make the analysis at night it results in that some samples are not analyzed until the morning. An automatic system could provide a microscopic analysis both day and night. After the bacteria sample has been cultivated it has to be stained to make the bacteria visible in a light microscopy. The m ...
MsEnod12A Expression Is Linked to Meristematic Activity
... 1992, and references therein). In alfalfa, even nodules developing spontaneously in the absence of rhizobia are of the indeterminate type harboring an apical meristem (Truchet et al. 1989). ...
... 1992, and references therein). In alfalfa, even nodules developing spontaneously in the absence of rhizobia are of the indeterminate type harboring an apical meristem (Truchet et al. 1989). ...
Fixation of Tissues for Routine Histology
... • Trim tissues to make plane of section obvious and well exposed to fixative. • Trim tissues to not exceed 2.5mm thickness • Trim tissues to remove any barrier membranes, which might slow fixation (ie. cortex of kidney, skin from muscle, etc.) • Perforate capsular membranes with a fine injection nee ...
... • Trim tissues to make plane of section obvious and well exposed to fixative. • Trim tissues to not exceed 2.5mm thickness • Trim tissues to remove any barrier membranes, which might slow fixation (ie. cortex of kidney, skin from muscle, etc.) • Perforate capsular membranes with a fine injection nee ...
Extended PDF
... that B. subtilis produces an additional biofilmdisassembly factor, norspermidine. Dynamic light scattering and scanning electron microscopy experiments indicated that norspermidine interacts directly and specifically with exopolysaccharide. D-amino acids and norspermidine acted together to break dow ...
... that B. subtilis produces an additional biofilmdisassembly factor, norspermidine. Dynamic light scattering and scanning electron microscopy experiments indicated that norspermidine interacts directly and specifically with exopolysaccharide. D-amino acids and norspermidine acted together to break dow ...
Dense Connective Tissues
... Cartilage is a tissue with poor blood supply that grows slowly. When injured or inflamed, repair is slow. Hyaline cartilage is the most abundant type of ...
... Cartilage is a tissue with poor blood supply that grows slowly. When injured or inflamed, repair is slow. Hyaline cartilage is the most abundant type of ...
Subcellular localization of peroxidase in tomato fruit skin and the
... Peroxidase (EC 1.11.1.7) is an oxidoreductase that is known to catalyse the oxidation of numerous substrates through the associated reduction of hydrogen peroxide (Dawson, 1988; Wallace and Fry, 1999). Given the ubiquitous presence of peroxidase throughout nature (VaÂmos-VigyaÂzoÂ, 1981) and the num ...
... Peroxidase (EC 1.11.1.7) is an oxidoreductase that is known to catalyse the oxidation of numerous substrates through the associated reduction of hydrogen peroxide (Dawson, 1988; Wallace and Fry, 1999). Given the ubiquitous presence of peroxidase throughout nature (VaÂmos-VigyaÂzoÂ, 1981) and the num ...
Some simple methods and tips for marine embryology
... unlike Propidium Iodide, no RNase treatment is necessary. Hoechst may even be useful on live cells, but I've never tried that. There are two commonly-used flavors of Hoechst: 33342 and 33258. The former is more soluble in water, and Molecular Probes claims it is more cell-permeant, but other than th ...
... unlike Propidium Iodide, no RNase treatment is necessary. Hoechst may even be useful on live cells, but I've never tried that. There are two commonly-used flavors of Hoechst: 33342 and 33258. The former is more soluble in water, and Molecular Probes claims it is more cell-permeant, but other than th ...
Clathrin in mitotic spindles - AJP-Cell
... addition, perinuclear staining is usually prominent; this staining pattern is thought to represent staining of the trans-Golgi network. In mitotic cells, punctate intracellular staining was also visible. However, unexpectedly, under the conditions employed here, mitotic spindles in both cell types w ...
... addition, perinuclear staining is usually prominent; this staining pattern is thought to represent staining of the trans-Golgi network. In mitotic cells, punctate intracellular staining was also visible. However, unexpectedly, under the conditions employed here, mitotic spindles in both cell types w ...
STUDIES ON THE MECHANISM OF THE PHOTOSENSITIZED
... increases to such a degree, that the amount of light reaching the cell-dye complex is drastically reduced. Inactivation of E. call-Bit as a Function of Exposure Time.--The dosesurvival relation is presented by curve 1 on Fig. 4. Such irradiation did not produce any measurable change in the absorptio ...
... increases to such a degree, that the amount of light reaching the cell-dye complex is drastically reduced. Inactivation of E. call-Bit as a Function of Exposure Time.--The dosesurvival relation is presented by curve 1 on Fig. 4. Such irradiation did not produce any measurable change in the absorptio ...
y or edat
... respectively. FDA diffuses across cells with intact membranes due to esterase activity. However once within active cells, the FDA substrate is cleaved by non-specific esterases releasing a polar fluorescein product that is retained inside cells with an intact membrane and the cells fluoresces green ...
... respectively. FDA diffuses across cells with intact membranes due to esterase activity. However once within active cells, the FDA substrate is cleaved by non-specific esterases releasing a polar fluorescein product that is retained inside cells with an intact membrane and the cells fluoresces green ...
Efficacy of Power-pulsed Lavage in Lower Extremity Wound
... postlavage cultures (methacillin-sensitive Staphylococcus aureus, methacillin-resistant S aureus, group B Streptococcus, Pseudomonas, Bacteroides, other flora); and whether or not the patient was already on a specific antibiotic based on culture and sensitivity results (yes or no). Specimens procured ...
... postlavage cultures (methacillin-sensitive Staphylococcus aureus, methacillin-resistant S aureus, group B Streptococcus, Pseudomonas, Bacteroides, other flora); and whether or not the patient was already on a specific antibiotic based on culture and sensitivity results (yes or no). Specimens procured ...
Intracranial Solitary Fibrous Tumor - Journal of the Belgian Society
... in a minority of the cases [1, 2]. Hypodense lesions have also been reported in a few cases [2]. In just more than half the cases, there was erosion of the skull adjacent to the tumour. There was intense enhancement after IV iodinated contrast injection. The reported enhancement patterns include hom ...
... in a minority of the cases [1, 2]. Hypodense lesions have also been reported in a few cases [2]. In just more than half the cases, there was erosion of the skull adjacent to the tumour. There was intense enhancement after IV iodinated contrast injection. The reported enhancement patterns include hom ...
Methods_Mol._Biol._591_185-199
... emission. As a result, only molecules that are close to the zero at the center of the “STED beam” are allowed to fluoresce and contribute to the fluorescence signal. Confining the signal to such a subdiffraction-sized spot results in higher resolution imaging when the spot is scanned through the spe ...
... emission. As a result, only molecules that are close to the zero at the center of the “STED beam” are allowed to fluoresce and contribute to the fluorescence signal. Confining the signal to such a subdiffraction-sized spot results in higher resolution imaging when the spot is scanned through the spe ...
Connective Tissue Proper: Dense Irregular
... the space between cells and contains the fibers • Fibers – provide support (collagen, elastic, or reticular) • Cells – fibroblasts (connective tissue proper), chondroblasts (cartilage), osteoblasts (bone), hematopoietic stem cells (produces blood cells), and accessory cells (mast cells = cluster alo ...
... the space between cells and contains the fibers • Fibers – provide support (collagen, elastic, or reticular) • Cells – fibroblasts (connective tissue proper), chondroblasts (cartilage), osteoblasts (bone), hematopoietic stem cells (produces blood cells), and accessory cells (mast cells = cluster alo ...
Autophagy is involved in starvation response and cell
... numerous membrane-bound vesicles containing organelles located within the central vacuole, resembling morphological features of autophagy. In this study, we investigated whether Blastocystis underwent autophagy upon amino acid starvation and rapamycin treatment. Concurrently, we provide new insight ...
... numerous membrane-bound vesicles containing organelles located within the central vacuole, resembling morphological features of autophagy. In this study, we investigated whether Blastocystis underwent autophagy upon amino acid starvation and rapamycin treatment. Concurrently, we provide new insight ...
The term fluorescence was coined by Stokes circa 1850 to name a
... ubiquitous intracellular esterases, releasing the ion-sensitive polyanionic indicator (see http://probes.invitrogen.com/handbook/boxes/0428.html). Cell-impermeable (salt) forms of Ca fluorophores can be loaded in the cytoplasm only via a micropipette attached to the cell. The esterified (AM) forms o ...
... ubiquitous intracellular esterases, releasing the ion-sensitive polyanionic indicator (see http://probes.invitrogen.com/handbook/boxes/0428.html). Cell-impermeable (salt) forms of Ca fluorophores can be loaded in the cytoplasm only via a micropipette attached to the cell. The esterified (AM) forms o ...
Embryonic origin and fate of chondroid tissue and
... When the quail encephalon is transplanted in the chick embryo, grafted cells give rise t o all skeletal deFigure 2 illustrates the main characteristics of the rivatives of the mandibular arch (Fig. 3). Indeed, at the various skeletal tissues involved in the development of 9th day of incubation, the ...
... When the quail encephalon is transplanted in the chick embryo, grafted cells give rise t o all skeletal deFigure 2 illustrates the main characteristics of the rivatives of the mandibular arch (Fig. 3). Indeed, at the various skeletal tissues involved in the development of 9th day of incubation, the ...
Staining
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.