ATPase in the plasma membrane of HeLa cells
... RT-PCR to examine the expression of four α-isoforms (α1-α4) and four βisoforms (β1-β4) of Na+/K+-ATPase. Primers used were designed to amplify an approximately 300 bp DNA fragment of each isoform cDNA (for the primer sequences, see supplementary material Table S1). The amplified fragment was subclon ...
... RT-PCR to examine the expression of four α-isoforms (α1-α4) and four βisoforms (β1-β4) of Na+/K+-ATPase. Primers used were designed to amplify an approximately 300 bp DNA fragment of each isoform cDNA (for the primer sequences, see supplementary material Table S1). The amplified fragment was subclon ...
The Cell - University of South Carolina
... and inclusions. It will explain how the more than 200 unique cell types in the body are derived from embryological origins and how different cell populations in the adult are maintained by mitosis and two means of cellular death. This lecture is intended to review cell structure and function for tho ...
... and inclusions. It will explain how the more than 200 unique cell types in the body are derived from embryological origins and how different cell populations in the adult are maintained by mitosis and two means of cellular death. This lecture is intended to review cell structure and function for tho ...
Simulating the Hallmarks of Cancer
... A more general view of cancer is outlined in Hanahan and Weinberg’s ‘‘The Hallmarks of Cancer’’ [10], which suggests that six cellular alterations are essential to malignant growth. These six hallmarks are believed to be common to most or all human tumors. Such a view offers the hope that our unders ...
... A more general view of cancer is outlined in Hanahan and Weinberg’s ‘‘The Hallmarks of Cancer’’ [10], which suggests that six cellular alterations are essential to malignant growth. These six hallmarks are believed to be common to most or all human tumors. Such a view offers the hope that our unders ...
Oncogene activation
... was kidnapped by an ancestor of RSV, incorporated by RSV into its own genome, and then expoloited by the virus to transform normal cells into cancer cells– “Jekyll-to-Hyde” ...
... was kidnapped by an ancestor of RSV, incorporated by RSV into its own genome, and then expoloited by the virus to transform normal cells into cancer cells– “Jekyll-to-Hyde” ...
Introduction: spatial origin of murine hematopoietic stem cells
... regarding the origin of HSCs during embryogenesis. AGM-S3 cells supported the generation of HSCs and spleen colony-forming unit cells (CFU-Ss) but not definitive colony-forming cells (CFCs). While both YS and P-Sp cells produced some CFCs during the first few days of coculture with the AGM-S3 cells, ...
... regarding the origin of HSCs during embryogenesis. AGM-S3 cells supported the generation of HSCs and spleen colony-forming unit cells (CFU-Ss) but not definitive colony-forming cells (CFCs). While both YS and P-Sp cells produced some CFCs during the first few days of coculture with the AGM-S3 cells, ...
- eScholarship@UMMS
... of the mononucleate cells in the same preparations continued cycling. By temporally separating the G1 arrest from the action of the drug, these workers provided evidence that the arrest was specific to the binucleate condition. Further indications that this arrest was due to failed cleavage came fro ...
... of the mononucleate cells in the same preparations continued cycling. By temporally separating the G1 arrest from the action of the drug, these workers provided evidence that the arrest was specific to the binucleate condition. Further indications that this arrest was due to failed cleavage came fro ...
Measuring the Mechanical Properties of Living Cells Using
... 7. Perform calibration of the InvOLS as described above, for the cantilever sensitivity in the liquid environment. Note: a) If cells are cultured on hydrogels, the calibration of InvOLS should be performed in advance against the bottom surface of a culture dish filled with cell culture media. When s ...
... 7. Perform calibration of the InvOLS as described above, for the cantilever sensitivity in the liquid environment. Note: a) If cells are cultured on hydrogels, the calibration of InvOLS should be performed in advance against the bottom surface of a culture dish filled with cell culture media. When s ...
In vitro development of inner cell masses isolated immunosurgically
... After 24-48 h incubation most of the inner cell masses isolated from half expanded blastocysts, and about 50 % of those from expanded blastocysts, have a striking resemblance to normal expanded blastocysts, with an outer trophectoderm-like wall surrounding an inner cluster of cells. Over the next fe ...
... After 24-48 h incubation most of the inner cell masses isolated from half expanded blastocysts, and about 50 % of those from expanded blastocysts, have a striking resemblance to normal expanded blastocysts, with an outer trophectoderm-like wall surrounding an inner cluster of cells. Over the next fe ...
A natural anti-T-cell receptor monoclonal antibody protects against
... were collected, double or triple stained for the indicated surface molecules, and analyzed by flow cytometry. Results are shown as the percentage of cells within the CD3+ population. a Mean ± SD for four mice in each group and representative of three independent experiments with similar results. ...
... were collected, double or triple stained for the indicated surface molecules, and analyzed by flow cytometry. Results are shown as the percentage of cells within the CD3+ population. a Mean ± SD for four mice in each group and representative of three independent experiments with similar results. ...
Biogenesis of MalF and the MalFGK Maltose Transport Escherichia coli 2
... both in association with the Sec translocon and separately (10, 11). Thus far, only a handful of inner membrane proteins that insert via the YidC-only pathway have been identified. All these proteins are small and do not contain a sizable periplasmic domains and more than two TMs. It is not clear ho ...
... both in association with the Sec translocon and separately (10, 11). Thus far, only a handful of inner membrane proteins that insert via the YidC-only pathway have been identified. All these proteins are small and do not contain a sizable periplasmic domains and more than two TMs. It is not clear ho ...
Nuclear hormone receptors (contd)
... initiates the muscle program. Later bHLH genes such as myogenin and myf5 are also important – this family of genes illustrates the general principle that combinatorial associations of transcription factors can yield complexes with different functions – DNA binding – transcriptional regulation ...
... initiates the muscle program. Later bHLH genes such as myogenin and myf5 are also important – this family of genes illustrates the general principle that combinatorial associations of transcription factors can yield complexes with different functions – DNA binding – transcriptional regulation ...
Dragonfly Chapter07
... 1. Some activities cannot be performed by only one person, but need a team of people. What type of activity requires a team of people to work together in order to complete a task? Answers might include building a human pyramid or constructing an arch out of blocks. 2. What do you think are some char ...
... 1. Some activities cannot be performed by only one person, but need a team of people. What type of activity requires a team of people to work together in order to complete a task? Answers might include building a human pyramid or constructing an arch out of blocks. 2. What do you think are some char ...
Nucleoids and coated vesicles of “Epulopiscium” spp.
... ter cells. For direct evidence of this proposed course of events it would, however, be necessary to examine Btypes from much earlier phases of the growth cycle than those represented in the samples that have been available to us. The DAPI preparations have also revealed instances of cells that had, ...
... ter cells. For direct evidence of this proposed course of events it would, however, be necessary to examine Btypes from much earlier phases of the growth cycle than those represented in the samples that have been available to us. The DAPI preparations have also revealed instances of cells that had, ...
Template for Electronic Submission to ACS Journals - Lirias
... detrimental to the folding process. These proteins, maturing faster and/or to a higher degree of completeness, are known as ‘superfolder’ FPs, and experimental strategies to generate these have been described previously.27,28 By observing the brightness of bacterial colonies expressing randomly muta ...
... detrimental to the folding process. These proteins, maturing faster and/or to a higher degree of completeness, are known as ‘superfolder’ FPs, and experimental strategies to generate these have been described previously.27,28 By observing the brightness of bacterial colonies expressing randomly muta ...
Transcription Domain-Associated Repair in Human Cells
... detection, and XP group C (XP-C) patients, deficient in global genomic repair (GGR), still retain TCR. Deficiencies in the TCR pathway can result in several other genetic diseases, including Cockayne syndrome, in which the patients are not cancer prone but suffer from developmental defects and numer ...
... detection, and XP group C (XP-C) patients, deficient in global genomic repair (GGR), still retain TCR. Deficiencies in the TCR pathway can result in several other genetic diseases, including Cockayne syndrome, in which the patients are not cancer prone but suffer from developmental defects and numer ...
AML1(7/7) embryos do not express certain hematopoiesis
... see a review by Leiden, 1993). The above claims, however, mostly rely on studies using cell lines transfected by reporter plasmids whose transcription is driven by the promoter of a suspected PEBP2/CBF regulated gene. In this study we have taken an ...
... see a review by Leiden, 1993). The above claims, however, mostly rely on studies using cell lines transfected by reporter plasmids whose transcription is driven by the promoter of a suspected PEBP2/CBF regulated gene. In this study we have taken an ...
Antigen recognition by T Lymphocytes
... Peptide loading on MHC • MHC Class I: intracellular antigens, e.g. viral proteins produced in virus-infected cells; peptide degradation in cytosol by proteasome, then transport to ER • MHC Class II: extracellular antigens, e.g. pathogen engulfed by phagocytes; degradation in phagosome and lysosome ...
... Peptide loading on MHC • MHC Class I: intracellular antigens, e.g. viral proteins produced in virus-infected cells; peptide degradation in cytosol by proteasome, then transport to ER • MHC Class II: extracellular antigens, e.g. pathogen engulfed by phagocytes; degradation in phagosome and lysosome ...
Cells and Organisation
... This plant cell also contains chloroplasts, a cell wall and a vacuole. Label each of these parts on the diagram. ...
... This plant cell also contains chloroplasts, a cell wall and a vacuole. Label each of these parts on the diagram. ...
Text S2.
... disease and Epithelial signaling in H. pilori infection in the oxidative metabolism cluster are worth an explanation, as these pathways have apparently little relation with metabolism. Alzheimer and Parkinson pathways include a large number of dehydrogenases (42 out 176 and 136 total genes respectiv ...
... disease and Epithelial signaling in H. pilori infection in the oxidative metabolism cluster are worth an explanation, as these pathways have apparently little relation with metabolism. Alzheimer and Parkinson pathways include a large number of dehydrogenases (42 out 176 and 136 total genes respectiv ...
4327
... morphological studies have documented that caveolae mediate the movement of molecules across endothelial cells (Simionescu, 1983). Transcytosis appears to involve membrane invagination, vesicle budding and movement of vesicles filled with cargo to the opposite side of the cell. The principle method ...
... morphological studies have documented that caveolae mediate the movement of molecules across endothelial cells (Simionescu, 1983). Transcytosis appears to involve membrane invagination, vesicle budding and movement of vesicles filled with cargo to the opposite side of the cell. The principle method ...
Chapter 5 Notes
... • The linear sequences of nucleotides in DNA molecules are passed from parents to offspring • Two closely related species are more similar in DNA than are more distantly related species • Molecular biology can be used to assess ...
... • The linear sequences of nucleotides in DNA molecules are passed from parents to offspring • Two closely related species are more similar in DNA than are more distantly related species • Molecular biology can be used to assess ...