Identification of a GDP-L-fucose: polypeptide fucosyltransferase and
... E.coli and purified to homogeneity. It migrated as a single peak on reversed phase HPLC, and preliminary NMR data confirmed that its conformation was similar to that of the native EGF-1 module (G.F.Lee and R.F.Kelley, unpublished observation). Experiments using factor VII EGF-1 as acceptor substrate ...
... E.coli and purified to homogeneity. It migrated as a single peak on reversed phase HPLC, and preliminary NMR data confirmed that its conformation was similar to that of the native EGF-1 module (G.F.Lee and R.F.Kelley, unpublished observation). Experiments using factor VII EGF-1 as acceptor substrate ...
Forces That Move Ions and Molecules
... lipid bilayer due to their size and/or polarity (Figure 5). A common example of facilitated diusion is the movement of glucose into the cell, where it is used to make ATP. Although glucose can be more concentrated outside of a cell, it cannot cross the lipid bilayer via simple diusion because it i ...
... lipid bilayer due to their size and/or polarity (Figure 5). A common example of facilitated diusion is the movement of glucose into the cell, where it is used to make ATP. Although glucose can be more concentrated outside of a cell, it cannot cross the lipid bilayer via simple diusion because it i ...
Probing the invasiveness of prostate cancer cells in a 3D
... be released to invade vacant areas and where their spatial motility and proliferation can be analyzed to compare the invasion potentials quantitatively. Our experiments do not involve flow but rather use three-dimensional microstructures of very high aspect ratios that provide an approach for the st ...
... be released to invade vacant areas and where their spatial motility and proliferation can be analyzed to compare the invasion potentials quantitatively. Our experiments do not involve flow but rather use three-dimensional microstructures of very high aspect ratios that provide an approach for the st ...
Somatic Cell Genealogies and Differentiation
... progressively increased until two years of age, and then became constant. The lack of age-related increase in hair methylation is consistent with infrequently-dividing stem cells because the average numbers of divisions during neogenesis and anagen are likely to be similar for all hairs. ...
... progressively increased until two years of age, and then became constant. The lack of age-related increase in hair methylation is consistent with infrequently-dividing stem cells because the average numbers of divisions during neogenesis and anagen are likely to be similar for all hairs. ...
Tasquinimod Modulates Suppressive Myeloid Cells and Enhances
... models (16–18). Results from these studies have suggested that the antiangiogenic property of this molecule may be responsible for its antitumor activity, because tumor growth inhibition was associated with reduced microvasculature density, increased expression, and secretion of the angiogenesis inh ...
... models (16–18). Results from these studies have suggested that the antiangiogenic property of this molecule may be responsible for its antitumor activity, because tumor growth inhibition was associated with reduced microvasculature density, increased expression, and secretion of the angiogenesis inh ...
10 m
... The cell cycle is driven by specific signaling molecules present in the cytoplasm Some evidence for this hypothesis comes from experiments with cultured mammalian cells Cells at different phases of the cell cycle were fused to form a single cell with two nuclei at different stages Cytoplasmi ...
... The cell cycle is driven by specific signaling molecules present in the cytoplasm Some evidence for this hypothesis comes from experiments with cultured mammalian cells Cells at different phases of the cell cycle were fused to form a single cell with two nuclei at different stages Cytoplasmi ...
Single Cell Electrical Characterization Techniques
... Received: 16 February 2015 / Accepted: 13 April 2015 / Published: 4 June 2015 ...
... Received: 16 February 2015 / Accepted: 13 April 2015 / Published: 4 June 2015 ...
1-Tubulin mRNAs Are Specified by the First 13
... whose RNAs encoded a fusion polypeptide consisting of 16 to 18 amino-terminal codons of fi tubulin linked to 278 amino acids of tk were constructed as follows. Mouse mP5 (26) and human H1l (encoded by gene M40; 25) tubulin sequences were obtained from mouse or human cDNA clones, subcloned into pUC9 ...
... whose RNAs encoded a fusion polypeptide consisting of 16 to 18 amino-terminal codons of fi tubulin linked to 278 amino acids of tk were constructed as follows. Mouse mP5 (26) and human H1l (encoded by gene M40; 25) tubulin sequences were obtained from mouse or human cDNA clones, subcloned into pUC9 ...
Non-protein-coding RNA
... The number of ncRNAs in an organism rises with its complexity. Just a small number of ncRNAs exist in prokaryotes, and the numbers of ncRNAs are higher in eukaryotes (Mattick and Makunin 2006). For many of the identified lincRNAs, no function has yet been discovered. The term “long ncRNA” applies w ...
... The number of ncRNAs in an organism rises with its complexity. Just a small number of ncRNAs exist in prokaryotes, and the numbers of ncRNAs are higher in eukaryotes (Mattick and Makunin 2006). For many of the identified lincRNAs, no function has yet been discovered. The term “long ncRNA” applies w ...
electrical coupling between fat cells in newt fat
... It is likely that many junctions were disrupted during the process of peeling away the surface layer of fat cells and adhering connective tissue . Furthermore, the coupling found in the amphibian fat was noticeably labile : frequently two cells, coupled when impaled with K2SO4 electrodes, were uncou ...
... It is likely that many junctions were disrupted during the process of peeling away the surface layer of fat cells and adhering connective tissue . Furthermore, the coupling found in the amphibian fat was noticeably labile : frequently two cells, coupled when impaled with K2SO4 electrodes, were uncou ...
Molecular Reaction Mechanisms of Proteins
... cm–1 and 1000 cm–1 during the photocycle are shown in a three-dimensional representation. Beyond a background absorbance of up to one absorbance unit, changes on the order of 10–3 to 10–4 are monitored with 3 cm–1 spectral resolution and 30 ns time resolution. The appearance of the C-C stretching vi ...
... cm–1 and 1000 cm–1 during the photocycle are shown in a three-dimensional representation. Beyond a background absorbance of up to one absorbance unit, changes on the order of 10–3 to 10–4 are monitored with 3 cm–1 spectral resolution and 30 ns time resolution. The appearance of the C-C stretching vi ...
Organelle Project - WLPCS Upper School
... An analogy with explanation: If the cell were a city or Washington Latin or a factory etc., what would this organelle be? (Ex. If the cell was restaurant, the nucleus would like a cookbook because it contains recipes (directions) for making many types of meals) ...
... An analogy with explanation: If the cell were a city or Washington Latin or a factory etc., what would this organelle be? (Ex. If the cell was restaurant, the nucleus would like a cookbook because it contains recipes (directions) for making many types of meals) ...
Visualizing_the_mechanical_activation_of_Src
... into cytoskeleton and do not induce FRET response Destruction of actin with cytochalasin D or microtubules with nocodazole blocked long range but not local FRET response ...
... into cytoskeleton and do not induce FRET response Destruction of actin with cytochalasin D or microtubules with nocodazole blocked long range but not local FRET response ...
Summary and Conclusion Facultyof Pharmacy Department of
... Diabetes is a major threat to global public that is associated with many complications threatening life. Diabetic nephropathy (DN) remains the most common cause for end stage renal disease. Adenosine is an endogenous purine nucleoside released from various tissues and organs. Adenosine controls the ...
... Diabetes is a major threat to global public that is associated with many complications threatening life. Diabetic nephropathy (DN) remains the most common cause for end stage renal disease. Adenosine is an endogenous purine nucleoside released from various tissues and organs. Adenosine controls the ...
The role of vacuole in plant cell death
... VPE-dependent hypersensitive cell death. Vacuolemediated cell death through destruction is initiated by a vacuolar enzyme, VPE.22,23,43 VPE-gene silencing completely suppresses lesion formation in TMV-infected leaves, vacuolar collapse, and DNA fragmentation. Although VPE deficiency prevents these t ...
... VPE-dependent hypersensitive cell death. Vacuolemediated cell death through destruction is initiated by a vacuolar enzyme, VPE.22,23,43 VPE-gene silencing completely suppresses lesion formation in TMV-infected leaves, vacuolar collapse, and DNA fragmentation. Although VPE deficiency prevents these t ...
Localization of the Microtubule End Binding Protein
... and Harper, 1998; Brown and Lemmon, 2001). However, in somatic cells, once the phragmoplast grows out beyond the internuclear corridor, it is not apparent where new microtubules originate, and the idea that microtubule plus ends overlap at the midline is not universally agreed upon: the possibility ...
... and Harper, 1998; Brown and Lemmon, 2001). However, in somatic cells, once the phragmoplast grows out beyond the internuclear corridor, it is not apparent where new microtubules originate, and the idea that microtubule plus ends overlap at the midline is not universally agreed upon: the possibility ...
The Aromatic-L-Amino Acid Decarboxylase Inhibitor Carbidopa Is
... observed with metastatic hepatic lesions), primary ileal carcinoid tumors and surrounding normal ileum samples were examined by Western blotting (Fig. 1B). The AAAD polypeptide level in the tumors was markedly higher than in their adjacent normal tissues (n ⫽ 3). Because the tissue quantity obtained ...
... observed with metastatic hepatic lesions), primary ileal carcinoid tumors and surrounding normal ileum samples were examined by Western blotting (Fig. 1B). The AAAD polypeptide level in the tumors was markedly higher than in their adjacent normal tissues (n ⫽ 3). Because the tissue quantity obtained ...
Herpes Simplex Virus Latency: The DNA Repair
... expression of the test protein was suppressed by treatment with specific siRNA. Controls in this case were performed with nonspecific siRNA. An example of the first type of study was performed with a cell line deficient in FANCA, a protein required for DNA repair and involved in the etiology of Fanc ...
... expression of the test protein was suppressed by treatment with specific siRNA. Controls in this case were performed with nonspecific siRNA. An example of the first type of study was performed with a cell line deficient in FANCA, a protein required for DNA repair and involved in the etiology of Fanc ...
The Protoplast: Plasma Membrane, Nucleus, and Cytoplasmic
... Among the internal membranes of eukaryotic cells are those surrounding the nucleus, mitochondria, and plastids, which are characteristic components of plant cells. The cytoplasm of eukaryotic cells also contains systems of membranes (the endoplasmic reticulum and Golgi apparatus) and a complex netwo ...
... Among the internal membranes of eukaryotic cells are those surrounding the nucleus, mitochondria, and plastids, which are characteristic components of plant cells. The cytoplasm of eukaryotic cells also contains systems of membranes (the endoplasmic reticulum and Golgi apparatus) and a complex netwo ...
BioWire_Progress_Report_Week_One_Rev_1
... Use photolithography techniques to create a “wire” of bacteria. Use cell-to-cell signaling and transcriptional regulation to create a chemical “pulse” that travels down the length of the wire. ...
... Use photolithography techniques to create a “wire” of bacteria. Use cell-to-cell signaling and transcriptional regulation to create a chemical “pulse” that travels down the length of the wire. ...
Review articles Transporter protein and drug resistance of
... polymorphism (SSCP) analysis showed a wild-type, mutant and mixed genotypes of TbAT1. Using SfaN1 restriction fragment length polymorphism (RFLP) and allele-specific PCR (AS-PCR), mutant TbAT1 and wild-type TbAT1 genes were isolated (Fig. 2). Mutant genes can lead to amino acid ...
... polymorphism (SSCP) analysis showed a wild-type, mutant and mixed genotypes of TbAT1. Using SfaN1 restriction fragment length polymorphism (RFLP) and allele-specific PCR (AS-PCR), mutant TbAT1 and wild-type TbAT1 genes were isolated (Fig. 2). Mutant genes can lead to amino acid ...
The proteome of the infectious bronchitis virus Beau
... each replicate (N – 144, 81 and 50; S – 21, 14 and 12; M – 11, 7 and 7). The fourth viral structural protein, E, the replicase proteins and the lineage-specific accessory proteins could not be identified. The total number of proteins identified in each preparation was 399, 289 and 273, respectively. ...
... each replicate (N – 144, 81 and 50; S – 21, 14 and 12; M – 11, 7 and 7). The fourth viral structural protein, E, the replicase proteins and the lineage-specific accessory proteins could not be identified. The total number of proteins identified in each preparation was 399, 289 and 273, respectively. ...
Arabidopsis whole-transcriptome profiling defines - Plant-o
... and Groups V and VI in the mature stage (Figure 3a and b). The putative functions of the genes were assigned and categorized according to the MIPS (http://mips.gsf.de/proj/thal/db/tables/ tables_func_frame.html). The distribution of the genes in each expression group appears to reflect the physiolog ...
... and Groups V and VI in the mature stage (Figure 3a and b). The putative functions of the genes were assigned and categorized according to the MIPS (http://mips.gsf.de/proj/thal/db/tables/ tables_func_frame.html). The distribution of the genes in each expression group appears to reflect the physiolog ...
Infectious bursal disease virus structural proteins expressed in a
... Fifteen ml of baculovirus- or recombinant baculovirusinfected cell lysate was emulsified with an equal volume of Freund's incomplete adjuvant and used for inoculation. To evaluate the protective properties of the recombinant proteins, 2-week-old white leghorn SPF chickens (SPAFAS, Inc.) were pre-ble ...
... Fifteen ml of baculovirus- or recombinant baculovirusinfected cell lysate was emulsified with an equal volume of Freund's incomplete adjuvant and used for inoculation. To evaluate the protective properties of the recombinant proteins, 2-week-old white leghorn SPF chickens (SPAFAS, Inc.) were pre-ble ...
A matter of size: developmental control of organ size in plants
... Arabidopsis thaliana plants grown under the same conditions are remarkably uniform in size. In contrast, the flowers and petals of Brassica napus, another member of Brassicaceae, are always much larger than those of Arabidopsis (Figure 1). Although it has long been established that organ size within ...
... Arabidopsis thaliana plants grown under the same conditions are remarkably uniform in size. In contrast, the flowers and petals of Brassica napus, another member of Brassicaceae, are always much larger than those of Arabidopsis (Figure 1). Although it has long been established that organ size within ...