Abstract: Listeria monocytogenes
... invasive than similar cultures grown without oxygen restriction. This means that not only the number of Listeria present in a given food item, but that also the physiological condition of these bacteria is important for food safety. The in vitro and in vivo data suggest that an oxygen-restricted L. ...
... invasive than similar cultures grown without oxygen restriction. This means that not only the number of Listeria present in a given food item, but that also the physiological condition of these bacteria is important for food safety. The in vitro and in vivo data suggest that an oxygen-restricted L. ...
Gram Stain
... components; the primary stain, a mordant that sets the stain, a decolorizing agent to remove the primary stain, and a counter stain. In the Gram stain, the primary stain is crystal violet. This gives the cell an intense purple color. The mordant, iodine, forms a complex with the crystal violet insid ...
... components; the primary stain, a mordant that sets the stain, a decolorizing agent to remove the primary stain, and a counter stain. In the Gram stain, the primary stain is crystal violet. This gives the cell an intense purple color. The mordant, iodine, forms a complex with the crystal violet insid ...
06_Lecture_Presentation
... Roles of the Cytoskeleton: Support, Motility, and Regulation • The cytoskeleton helps to support the cell and maintain its shape • It interacts with motor proteins to produce motility • Inside the cell, vesicles can travel along “monorails” provided by the cytoskeleton • Recent evidence suggests th ...
... Roles of the Cytoskeleton: Support, Motility, and Regulation • The cytoskeleton helps to support the cell and maintain its shape • It interacts with motor proteins to produce motility • Inside the cell, vesicles can travel along “monorails” provided by the cytoskeleton • Recent evidence suggests th ...
Protection of Retinal Ganglion Cells from Natural and Axotomy
... Animals. The production of transgenic mice has been described previously (Martinou et al., 1994). Two transgenic lines were used in this study: neuron-specific enolase promoter (NSE) 71 and NSE 73a/b. Males heterozygous for the transgene were crossed with C57 Bl/6 (wild-type) females. The presence o ...
... Animals. The production of transgenic mice has been described previously (Martinou et al., 1994). Two transgenic lines were used in this study: neuron-specific enolase promoter (NSE) 71 and NSE 73a/b. Males heterozygous for the transgene were crossed with C57 Bl/6 (wild-type) females. The presence o ...
Glycogen, Amylase and tx-Glucosidase as Possible Components in
... TVo^toc filaments (20-50 g wet weight) were washed once with 0.75 M potassium phosphate buffer p H 7.0, resuspended with the help of a glass potter in fresh phosphate buffer at a density of 0.5 g wet weight/ml and were passed twice through a French press. The homogenate was centrifuged at 48,000 g f ...
... TVo^toc filaments (20-50 g wet weight) were washed once with 0.75 M potassium phosphate buffer p H 7.0, resuspended with the help of a glass potter in fresh phosphate buffer at a density of 0.5 g wet weight/ml and were passed twice through a French press. The homogenate was centrifuged at 48,000 g f ...
The chemokine SDF1/CXCL12 and its receptor CXCR4 regulate
... (C) Cxcr4 message is enriched in PGCs relative to the somatic tissue. The level of Cxcr4 transcripts in E11.5 PGCs (GFP+) or somatic tissue (GFP–) were quantified by SYBR-green based RT-PCR. The meiotic marker, STAG3 (Pezzi et al., 2000) was used as a positive control for PGCs, and the gonadal marke ...
... (C) Cxcr4 message is enriched in PGCs relative to the somatic tissue. The level of Cxcr4 transcripts in E11.5 PGCs (GFP+) or somatic tissue (GFP–) were quantified by SYBR-green based RT-PCR. The meiotic marker, STAG3 (Pezzi et al., 2000) was used as a positive control for PGCs, and the gonadal marke ...
Activation of the Protein Kinase Akt/PKB by the
... To explore the possibility that E-cadherin engagement leads to Akt activation, MDCK cells were grown as long confluent cultures by maintaining cells in complete medium for at least 24-48 h after reaching confluence, to optimize cell-cell contacts as well as to minimize the influence of integrin-extr ...
... To explore the possibility that E-cadherin engagement leads to Akt activation, MDCK cells were grown as long confluent cultures by maintaining cells in complete medium for at least 24-48 h after reaching confluence, to optimize cell-cell contacts as well as to minimize the influence of integrin-extr ...
Mutations in the Drosophila Condensin Subunit
... camera and imaging software. The effect of dcap-g alleles on the regulation of gene expression by the Polycomb-group response element was studied as described (Lupo et al. 2001) using dcap-g K3 and dcap-g K1 alleles. ...
... camera and imaging software. The effect of dcap-g alleles on the regulation of gene expression by the Polycomb-group response element was studied as described (Lupo et al. 2001) using dcap-g K3 and dcap-g K1 alleles. ...
neuropeptide y is a factor secreted by human endocardial
... Department of Anatomy and Cell Biology, Faculty of medicine, University of Sherbrooke, Sherbrooke, Québec, Canada, J1H 5N4. Very recently, we showed that NPY receptors are present in right ventricular endocardial endothelial cells (EECs) and that the activation of these receptors modulate cytosolic ...
... Department of Anatomy and Cell Biology, Faculty of medicine, University of Sherbrooke, Sherbrooke, Québec, Canada, J1H 5N4. Very recently, we showed that NPY receptors are present in right ventricular endocardial endothelial cells (EECs) and that the activation of these receptors modulate cytosolic ...
Redox sensing and signalling associated with - Mi Portal
... Kiddle et al. 2003). There is little doubt that other redox signalling molecules remain to be discovered. For example, the membrane-bound peroxiredoxins (PRX), whose main role may be protection against lipid peroxidation, have all the necessary molecular characteristics of thiol Physiol. Plant. 119, ...
... Kiddle et al. 2003). There is little doubt that other redox signalling molecules remain to be discovered. For example, the membrane-bound peroxiredoxins (PRX), whose main role may be protection against lipid peroxidation, have all the necessary molecular characteristics of thiol Physiol. Plant. 119, ...
Human stem cell aging: do mitochondrial DNA mutations have a
... Somatic MtDNA mutations in aging human mitotic tissues MtDNA mutations were initially detected in aging human postmitotic tissues, with a mosaic pattern of COX deficiency seen in the heart (Muller-Hocker, 1989), muscle (Muller-Hocker, 1990), and brain (Cottrell et al., 2001). More recently, respirat ...
... Somatic MtDNA mutations in aging human mitotic tissues MtDNA mutations were initially detected in aging human postmitotic tissues, with a mosaic pattern of COX deficiency seen in the heart (Muller-Hocker, 1989), muscle (Muller-Hocker, 1990), and brain (Cottrell et al., 2001). More recently, respirat ...
Arbonne-Protein-Shake-Label-Avery
... Arbonne Protein Shake (Chocolate – Vanilla) Add contents to 9 oz. of cold water or almond milk and shake vigorously (add ice and use a blender for a thicker shake). ...
... Arbonne Protein Shake (Chocolate – Vanilla) Add contents to 9 oz. of cold water or almond milk and shake vigorously (add ice and use a blender for a thicker shake). ...
Partitioning of nutrient transport processes in roots
... Potassium transporters of the KUPuHAK subtypes have been isolated from a variety of plants and their structural and sequence similarities indicate that they form a family of related transporters (Rubio et al., 2000). They have 12 membrane-spanning domains and a long cytosolic C-terminal domain (Kim ...
... Potassium transporters of the KUPuHAK subtypes have been isolated from a variety of plants and their structural and sequence similarities indicate that they form a family of related transporters (Rubio et al., 2000). They have 12 membrane-spanning domains and a long cytosolic C-terminal domain (Kim ...
Molten rocks in motion
... pass through molten rocks and electrical conductivity is much higher in magma than in solid rocks. In the MELT area, seismic data9 indicate that small amounts of magma (* 2%) are asymmetrically distributed around the ridge axis, in a region extending much farther to the west (up to 350 km) than to t ...
... pass through molten rocks and electrical conductivity is much higher in magma than in solid rocks. In the MELT area, seismic data9 indicate that small amounts of magma (* 2%) are asymmetrically distributed around the ridge axis, in a region extending much farther to the west (up to 350 km) than to t ...
Physico-chemical characteristics of cell walls from Arabidopsis
... percentage of total wall galactose released by the endo(1,4)-b-D-galactanase gradually decreased during the cell culture. The a-L-arabinofuranosidase released about 50% of total wall arabinose at the three growth phases, but bD-galactosidase released no galactose. In order to test whether these enzy ...
... percentage of total wall galactose released by the endo(1,4)-b-D-galactanase gradually decreased during the cell culture. The a-L-arabinofuranosidase released about 50% of total wall arabinose at the three growth phases, but bD-galactosidase released no galactose. In order to test whether these enzy ...
mechanotransduction in lymphatic endothelial cells
... and Fos, act on the promoter of genes for transcriptional modulation (27). In synthesis, MAP kinases convert the short duration membrane signals of tyrosine-phosphorylation in long lasting serine-threonine phosphorylations inside the cell toward the nucleus. Recent data indicate that ERKs dimerize i ...
... and Fos, act on the promoter of genes for transcriptional modulation (27). In synthesis, MAP kinases convert the short duration membrane signals of tyrosine-phosphorylation in long lasting serine-threonine phosphorylations inside the cell toward the nucleus. Recent data indicate that ERKs dimerize i ...
Cancer Chemopreventive Potential of Scenedesmus spp. Cultured
... protective device against quinone toxicity by reducing quinones to relatively stable hydroquinones. These resulting hydroquinones can be conjugated and excreted through mercapturic acid pathways. GST catalyzes the conjugation of electrophilic compounds with glutathione, resulting in soluble complexe ...
... protective device against quinone toxicity by reducing quinones to relatively stable hydroquinones. These resulting hydroquinones can be conjugated and excreted through mercapturic acid pathways. GST catalyzes the conjugation of electrophilic compounds with glutathione, resulting in soluble complexe ...
Epstein-Barr virus inhibits the development of dendritic cells by
... infected with the prototype B95.8 virus and then cultured for 7 days in the presence of GM-CSF and IL-4. Cells expressing typical DC morphology and cell surface markers, including high levels of MHC class II and CD86, were recovered from the infected cultures, but a dramatic decrease in cell number ...
... infected with the prototype B95.8 virus and then cultured for 7 days in the presence of GM-CSF and IL-4. Cells expressing typical DC morphology and cell surface markers, including high levels of MHC class II and CD86, were recovered from the infected cultures, but a dramatic decrease in cell number ...
Comparison of cryofixation and aldehyde fixation for plant actin
... (E, F, I, J), and embedded in Steedman’s wax. Formaldehyde-fixed samples show identical actin staining patterns to those obtained in cryofixed samples. Shown are cells of cortex (A–F), epidermis (G, I), and stele (H, J). Photographs were taken using an epifluorescence microscope. Scale bar in J = 10 ...
... (E, F, I, J), and embedded in Steedman’s wax. Formaldehyde-fixed samples show identical actin staining patterns to those obtained in cryofixed samples. Shown are cells of cortex (A–F), epidermis (G, I), and stele (H, J). Photographs were taken using an epifluorescence microscope. Scale bar in J = 10 ...
CELLULAR ADAPTATION TO AMINO ACID AVAILABILITY:
... • Regulation of the human CHOP gene by amino acid starvation CHOP encodes a ubiquitous transcription factor that heterodimerises avidly with the other members of the C/EBP (Fawcett et al. 1996) and jun/fos (Ubeda et al. 1999) family. The CHOP gene is tightly regulated by a wide variety of stresses i ...
... • Regulation of the human CHOP gene by amino acid starvation CHOP encodes a ubiquitous transcription factor that heterodimerises avidly with the other members of the C/EBP (Fawcett et al. 1996) and jun/fos (Ubeda et al. 1999) family. The CHOP gene is tightly regulated by a wide variety of stresses i ...
Character and origin of vacuoles induced in mammalian cells by the
... observation of intravacuolar acid phosphatase activity, provides evidence that vacuoles communicate at some point during their development with endocytically derived compartments. These observations provide direct evidencefor an autophagic origin of these structures. ...
... observation of intravacuolar acid phosphatase activity, provides evidence that vacuoles communicate at some point during their development with endocytically derived compartments. These observations provide direct evidencefor an autophagic origin of these structures. ...
Mutations in the conserved carboxy-terminal hydrophobic region of
... nuclear envelope. Complementation with a gB null-virus showed that mutants G746N, G766N, F770S and P774L showed negligible infectivity whereas G743R showed reduced infectivity. Virus particles generated from the complementation experiment containing these mutant glycoproteins also showed a markedly ...
... nuclear envelope. Complementation with a gB null-virus showed that mutants G746N, G766N, F770S and P774L showed negligible infectivity whereas G743R showed reduced infectivity. Virus particles generated from the complementation experiment containing these mutant glycoproteins also showed a markedly ...
Detecting RNA viruses in living mammalian cells by fluorescence microscopy Author's personal copy
... target RNA. The feasibility of tracking viral infection has been successfully demonstrated for paramyxovirus [18]. Although the signal to noise ratio is relatively modest, the small size of the MTRIPs and their ability to fluoresce at multiple wavelengths makes them a potential candidate for trackin ...
... target RNA. The feasibility of tracking viral infection has been successfully demonstrated for paramyxovirus [18]. Although the signal to noise ratio is relatively modest, the small size of the MTRIPs and their ability to fluoresce at multiple wavelengths makes them a potential candidate for trackin ...
Pathway in Gliomas Cannabinoids Inhibit the Vascular Endothelial
... Cannabinoids, the active components of Cannabis sativa L. (marijuana), and their derivatives exert a wide array of effects by activating their specific G protein-coupled receptors CB1 and CB2, which are normally engaged by a family of endogenous ligands–the endocannabinoids (9, 10). Marijuana and it ...
... Cannabinoids, the active components of Cannabis sativa L. (marijuana), and their derivatives exert a wide array of effects by activating their specific G protein-coupled receptors CB1 and CB2, which are normally engaged by a family of endogenous ligands–the endocannabinoids (9, 10). Marijuana and it ...
Article PDF
... of oncogenes. Metabolic profiling revealed a progressive loss of respiration and an accompanying dependence on glycolysis for cell growth. The final introduction of the ras oncogene, which reflects a genetic aberration commonly observed in a variety of tumours, ultimately boosted resistance to oligo ...
... of oncogenes. Metabolic profiling revealed a progressive loss of respiration and an accompanying dependence on glycolysis for cell growth. The final introduction of the ras oncogene, which reflects a genetic aberration commonly observed in a variety of tumours, ultimately boosted resistance to oligo ...