Localization of growth and secretion of proteins in
... For detection of proteins secreted into the medium, the growing sandwiched colony was briefly lifted from the nutrient agar and a wetted polyvinylidenedifluoride (PVDF) membrane (Millipore) was positioned between the lower polycarbonate membrane and the agar medium. Glucoamylase was detected on the ...
... For detection of proteins secreted into the medium, the growing sandwiched colony was briefly lifted from the nutrient agar and a wetted polyvinylidenedifluoride (PVDF) membrane (Millipore) was positioned between the lower polycarbonate membrane and the agar medium. Glucoamylase was detected on the ...
Extracellular Matrix Components Regulate Cellular Polarity and
... mediated by transmembrane cell surface receptors which connect the cell’s cytoskeleton with the extracellular environment, leading to the formation of site-specific focal adhesions.[11,12] The extracellular cues established by binding of cells to the ECM are propagated to the nucleus from the cell s ...
... mediated by transmembrane cell surface receptors which connect the cell’s cytoskeleton with the extracellular environment, leading to the formation of site-specific focal adhesions.[11,12] The extracellular cues established by binding of cells to the ECM are propagated to the nucleus from the cell s ...
Comparing Automated and Manual Cell Counts for Cell Culture
... the 15–25% range. Beckman Coulter claims a counting accuracy of ±6% using well-defined particles such as polystyrene beads (6). That level of accuracy is difficult to obtain in practice with cell culture samples, which are more heterogeneous because of their wider size distribution and the presence ...
... the 15–25% range. Beckman Coulter claims a counting accuracy of ±6% using well-defined particles such as polystyrene beads (6). That level of accuracy is difficult to obtain in practice with cell culture samples, which are more heterogeneous because of their wider size distribution and the presence ...
Structure of the Reovirus Membrane
... and I and the exposed bridge between them project laterally, forming a cradle for the base of 3. The exposed bridge contains the ␦/φ cleavage sites C-terminal to residue 581 (cleavage with chymotrypsin) and 584 (cleavage with trypsin) (Nibert and Fields, 1992). Domain IV: The head domain at the tip ...
... and I and the exposed bridge between them project laterally, forming a cradle for the base of 3. The exposed bridge contains the ␦/φ cleavage sites C-terminal to residue 581 (cleavage with chymotrypsin) and 584 (cleavage with trypsin) (Nibert and Fields, 1992). Domain IV: The head domain at the tip ...
Anatomical variation of filum terminale fusion and dural
... Cricoid: C6, hyaline, only complete ring therefore can compress the esophagus Triticeal Epiglottis: extends to C3, elastic Cuneiform: elastic, does not contact other cartilages Corniculate: limited function in man, elastic Arytenoid: ½ elastic ½ hyaline By ~age 65 thyroid, cricoid and ...
... Cricoid: C6, hyaline, only complete ring therefore can compress the esophagus Triticeal Epiglottis: extends to C3, elastic Cuneiform: elastic, does not contact other cartilages Corniculate: limited function in man, elastic Arytenoid: ½ elastic ½ hyaline By ~age 65 thyroid, cricoid and ...
Recent advances in plant cell wall proteomics
... classical secretory pathway [39]. A sequence-based method of prediction of non-classicaltriggered secretion for mammalian proteins, gram+, and gram- bacteria, has been developed (http://www.cbs.dtu.dk/services/SecretomeP-1.0). The assumption is that extracellular proteins share certain properties an ...
... classical secretory pathway [39]. A sequence-based method of prediction of non-classicaltriggered secretion for mammalian proteins, gram+, and gram- bacteria, has been developed (http://www.cbs.dtu.dk/services/SecretomeP-1.0). The assumption is that extracellular proteins share certain properties an ...
Promotive effect of brassinosteroids on cell division involves a
... the late S to G2 phase, before being destroyed later in mitosis (Pines and Hunter, 1990). The complex formed by cyclin D or E, with their associated CDKs, directly phosphorylates the retinoblastoma (Rb) protein in the mid- to late-G1 phase, thereby driving cells across the G1/S boundary (Sherr, 1996 ...
... the late S to G2 phase, before being destroyed later in mitosis (Pines and Hunter, 1990). The complex formed by cyclin D or E, with their associated CDKs, directly phosphorylates the retinoblastoma (Rb) protein in the mid- to late-G1 phase, thereby driving cells across the G1/S boundary (Sherr, 1996 ...
Retention of Glucose Units Added by the UDP
... of them were twice washed with H a m ' s F-12 (methionine, proline, and glycine free) medium (10.65 g per liter) supplemented with 34.5 mg per liter of proline and 7.5 nag per liter of glycine and 1.2 g per liter of NaHCO3. The parasites were resuspended in 9 ml of the above indicated medium. The su ...
... of them were twice washed with H a m ' s F-12 (methionine, proline, and glycine free) medium (10.65 g per liter) supplemented with 34.5 mg per liter of proline and 7.5 nag per liter of glycine and 1.2 g per liter of NaHCO3. The parasites were resuspended in 9 ml of the above indicated medium. The su ...
File
... thought to be the first organisms to live on Earth. They do not have a nucleus, and can be up to 200 times smaller than eukaryotes. Bacteria are examples of prokaryotes. They come in different shapes and sizes, live in different environments and have a range of food ...
... thought to be the first organisms to live on Earth. They do not have a nucleus, and can be up to 200 times smaller than eukaryotes. Bacteria are examples of prokaryotes. They come in different shapes and sizes, live in different environments and have a range of food ...
Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells
... (Figures 2–4; Supplemental Materials 2–4) allows us to conclude that both β-1,4-glucosidases and β-1,4glucanases are active during secondary wall development in sclerenchyma cells, but that β-1,4-glucanases are less abundant or less active, at least against these artificial fluorogenic substrates. I ...
... (Figures 2–4; Supplemental Materials 2–4) allows us to conclude that both β-1,4-glucosidases and β-1,4glucanases are active during secondary wall development in sclerenchyma cells, but that β-1,4-glucanases are less abundant or less active, at least against these artificial fluorogenic substrates. I ...
Eye - iupui
... neural retina. The potential space between the outer and inner epithelium is site of retinal detachment. The optic part is comprised of 10 layers containing 2 areas of specialization: optic disc – is the site of exit of optic “nerve” (CN II) and entry of central retinal artery and vein; this area is ...
... neural retina. The potential space between the outer and inner epithelium is site of retinal detachment. The optic part is comprised of 10 layers containing 2 areas of specialization: optic disc – is the site of exit of optic “nerve” (CN II) and entry of central retinal artery and vein; this area is ...
Chapter 3—Cells
... Identify two functions of the Golgi complex and describe how it performs those functions. ANS: The Golgi complex packages and distributes proteins. It packages proteins in small bubbles made of a piece of membrane. The bubbles then break off and transport their contents to other parts of the cell. ...
... Identify two functions of the Golgi complex and describe how it performs those functions. ANS: The Golgi complex packages and distributes proteins. It packages proteins in small bubbles made of a piece of membrane. The bubbles then break off and transport their contents to other parts of the cell. ...
Urine formation by kidney.
... glomerular filtration for their excretion. • Most waste products are poorly reabsorbed by the tubules and depend on a high GFR for effective removal. • Plasma volume is only about 3 liters, whereas the GFR is about 180 L/day, the entire plasma can be filtered and processed about 60 times each day. • ...
... glomerular filtration for their excretion. • Most waste products are poorly reabsorbed by the tubules and depend on a high GFR for effective removal. • Plasma volume is only about 3 liters, whereas the GFR is about 180 L/day, the entire plasma can be filtered and processed about 60 times each day. • ...
CYCLIC CHANGES IN THE CELL SURFACE I. Change in
... After fixation cover slips were extracted with I N HCI and dehydrated through ethanol . The dried cover slips were then counted in a windowless gas-flow planchet counter. The techniques for measuring incorporation of radioactive precursors into macromolecules in cover slip cultures is described in d ...
... After fixation cover slips were extracted with I N HCI and dehydrated through ethanol . The dried cover slips were then counted in a windowless gas-flow planchet counter. The techniques for measuring incorporation of radioactive precursors into macromolecules in cover slip cultures is described in d ...
Photobleaching Substrates Characterized Using Fluorescence
... Similarly, Ig heavy chain class switch recombination, which also contains a DSB intermediate, is similarly inhibited in Ku70-deficient mice (18). Despite the evidence that Ku functions in NHEJ during repair of DSBs, its properties in intact cells are unclear. For example, little is known concerning ...
... Similarly, Ig heavy chain class switch recombination, which also contains a DSB intermediate, is similarly inhibited in Ku70-deficient mice (18). Despite the evidence that Ku functions in NHEJ during repair of DSBs, its properties in intact cells are unclear. For example, little is known concerning ...
Lipid raft–associated protein sorting in exosomes
... process or at least not the sole one, since (1) lipids incorporated into the external leaflet of the plasma membrane are very efficiently sorted and secreted in association with exosomes,23 (2) acetylcholinesterase (AChE), a glycosylphosphatidylinositol (GPI)– anchored protein in red cells, is simil ...
... process or at least not the sole one, since (1) lipids incorporated into the external leaflet of the plasma membrane are very efficiently sorted and secreted in association with exosomes,23 (2) acetylcholinesterase (AChE), a glycosylphosphatidylinositol (GPI)– anchored protein in red cells, is simil ...
PDF
... 2008; Olmedo-Verd et al., 2011). Membrane anchoring was particularly well demonstrated for the Valyl-tRNA synthetase (ValRS) of Anabaena sp. PCC 7120 (hereafter Anabaena), which harbors an idiosyncratic domain, termed CAAD, with a putative C-terminal coiled-coil and two transmembrane helices, that m ...
... 2008; Olmedo-Verd et al., 2011). Membrane anchoring was particularly well demonstrated for the Valyl-tRNA synthetase (ValRS) of Anabaena sp. PCC 7120 (hereafter Anabaena), which harbors an idiosyncratic domain, termed CAAD, with a putative C-terminal coiled-coil and two transmembrane helices, that m ...
Nanoscale Architecture of Endoplasmic Reticulum Export Sites and
... Sec13:GFP, a COPII coat protein and ER export site marker, produced ER-associated fluorescent punctate structures that had an extremely short half-life of only ,10 s and seemed to form randomly over the ER. The number of such structures was also greater than the number of Golgi stacks, and the Golgi ...
... Sec13:GFP, a COPII coat protein and ER export site marker, produced ER-associated fluorescent punctate structures that had an extremely short half-life of only ,10 s and seemed to form randomly over the ER. The number of such structures was also greater than the number of Golgi stacks, and the Golgi ...
BIOLOGY
... response, and not a behavioral response, because a) it involves the formation of new permanent structures and molecules. b) the response is done upon the detection of light. c) there is the involvement of complex transduction mechanisms. d) two sets of protein kinases are involved in transduction. e ...
... response, and not a behavioral response, because a) it involves the formation of new permanent structures and molecules. b) the response is done upon the detection of light. c) there is the involvement of complex transduction mechanisms. d) two sets of protein kinases are involved in transduction. e ...
The Myriad Roles of Anillin during Cytokinesis Alisa J. Piekny1 and
... F-actin and Anillin are recruited independently to the contractile ring in human and Drosophila cultured cells, as well as to meiotic rings in Drosophila spermatogenesis [11-14]. However, F-actin increases the efficiency of Anillin targeting to the cell equator in both systems (in terms of timing or ...
... F-actin and Anillin are recruited independently to the contractile ring in human and Drosophila cultured cells, as well as to meiotic rings in Drosophila spermatogenesis [11-14]. However, F-actin increases the efficiency of Anillin targeting to the cell equator in both systems (in terms of timing or ...
Document
... Animal cells and plant cells have features in common, such as a nucleus, cytoplasm, cell membrane, mitochondria and ribosomes. Plant and algal cells also have a cell wall, and often have chloroplasts and a permanent vacuole. Bacterial and yeast cells have different structures to animal and plant cel ...
... Animal cells and plant cells have features in common, such as a nucleus, cytoplasm, cell membrane, mitochondria and ribosomes. Plant and algal cells also have a cell wall, and often have chloroplasts and a permanent vacuole. Bacterial and yeast cells have different structures to animal and plant cel ...