Cloning in farm animals: Concepts and applications
... development (Brigg and King, 1952). In1973, Boyer and Cohen created the first recombinant DNA organism using recombinant DNA technology, or gene-splicing, which allow the manipulation of DNA. They remove plasmid from a cell. Using restriction enzymes, they cut the DNA at precise positions and then r ...
... development (Brigg and King, 1952). In1973, Boyer and Cohen created the first recombinant DNA organism using recombinant DNA technology, or gene-splicing, which allow the manipulation of DNA. They remove plasmid from a cell. Using restriction enzymes, they cut the DNA at precise positions and then r ...
Lecture 15 - People Server at UNCW
... Regardless of their environment, all animals must be capable of maintaining isosmoticity between the ICF (intracellular fluid) and ECF (extracellular fluid). ...
... Regardless of their environment, all animals must be capable of maintaining isosmoticity between the ICF (intracellular fluid) and ECF (extracellular fluid). ...
Polarization of Endocytosis and Receptor
... times with either monolayers or suspensions of 3774.2 cells . The cells were rinsed in PBS, fixed in 2% glutaraldehyde in 0.1 M cacodylate buffer pH 7.4, washed in cacodylate buffer, postfixed in 1% osmium tetroxide, and then dehydrated through a graded series of ethanols as described before (7). In ...
... times with either monolayers or suspensions of 3774.2 cells . The cells were rinsed in PBS, fixed in 2% glutaraldehyde in 0.1 M cacodylate buffer pH 7.4, washed in cacodylate buffer, postfixed in 1% osmium tetroxide, and then dehydrated through a graded series of ethanols as described before (7). In ...
Heat Shock Proteins and Neurodegenerative Disorders
... Heat shock proteins (HSPs), also known as stress proteins, were first discovered in 1962 by Ritossa[1], who observed that a transient increase in temperature induced puffing patterns in the chromosomes of salivary glands of Drosophila melanogaster larvae. Temperature increases were subsequently foun ...
... Heat shock proteins (HSPs), also known as stress proteins, were first discovered in 1962 by Ritossa[1], who observed that a transient increase in temperature induced puffing patterns in the chromosomes of salivary glands of Drosophila melanogaster larvae. Temperature increases were subsequently foun ...
Nuclear centering in Spirogyra
... inner side of the chloroplast envelope (Fig. 5B). In the thicker, proximal parts of the PNS the stalks comprised a bundle of parallel MTs (or MT bundles) which, however, could be resolved only if the specimen had been detergent-treated (Fig. 5B±D). The bundles split upon distal branching of the stal ...
... inner side of the chloroplast envelope (Fig. 5B). In the thicker, proximal parts of the PNS the stalks comprised a bundle of parallel MTs (or MT bundles) which, however, could be resolved only if the specimen had been detergent-treated (Fig. 5B±D). The bundles split upon distal branching of the stal ...
Root cytoskeleton: its role in perception of and response to gravity
... gravity is also indicated by their active role in signal transduction across the cell periphery (Sastry and Horwitz 1993; Pavalko and Otey 1994) and in controlling the spatial organization of MFs (Miyamoto et al. 1995). Importantly, the integrity of F-actin networks was shown to aect formation of t ...
... gravity is also indicated by their active role in signal transduction across the cell periphery (Sastry and Horwitz 1993; Pavalko and Otey 1994) and in controlling the spatial organization of MFs (Miyamoto et al. 1995). Importantly, the integrity of F-actin networks was shown to aect formation of t ...
UNDERSTANDING THE INTRACELLULAR NICHE IN CNIDARIAN
... the new host. Humans can be infected either by oocysts that contaminate food, or by tachy/bradyzoites that they ingest from undercooked meat infected with Toxoplasma. An overview of Toxoplasma’s subcellular structure and lytic cycle (described below) is illustrated in Fig. 1. Invasion and manipulati ...
... the new host. Humans can be infected either by oocysts that contaminate food, or by tachy/bradyzoites that they ingest from undercooked meat infected with Toxoplasma. An overview of Toxoplasma’s subcellular structure and lytic cycle (described below) is illustrated in Fig. 1. Invasion and manipulati ...
A comparison between nuclear dismantling during plant and animal
... directions [16]. The nuclear matrix at the internal side of the nuclear envelope acts as a skeleton defining nuclear size and shape. Several nuclear membrane proteins localized to the inner side of the nuclear envelope provide binding sites for chromatin and nuclear matrix in animals and plants. In ...
... directions [16]. The nuclear matrix at the internal side of the nuclear envelope acts as a skeleton defining nuclear size and shape. Several nuclear membrane proteins localized to the inner side of the nuclear envelope provide binding sites for chromatin and nuclear matrix in animals and plants. In ...
Chapter 03: The Neuronal Membrane at Rest
... Copyright © 2007 Wolters Kluwer Health | Lippincott Williams & Wilkins ...
... Copyright © 2007 Wolters Kluwer Health | Lippincott Williams & Wilkins ...
Full-Text PDF
... cargo-specific receptor interactions resulting in a signaling cascade that triggers cytoskeletal rearrangements. This causes formation of cell surface extensions that specifically zipper up around the cargo and form a cargo-sized vacuole called the phagosome [66]. Dynamin-2 has been reported to be r ...
... cargo-specific receptor interactions resulting in a signaling cascade that triggers cytoskeletal rearrangements. This causes formation of cell surface extensions that specifically zipper up around the cargo and form a cargo-sized vacuole called the phagosome [66]. Dynamin-2 has been reported to be r ...
1 Video-microscopic imaging of cell spatio
... factors involved in these processes are numerous and closely interacting. Cell migration involves protrusion of the plasma membrane (lamellipodium extension) at the leading edge of the cell, which implies cytoskeleton reorganization such as actin [10] or vimentin, the major intermediate filament pro ...
... factors involved in these processes are numerous and closely interacting. Cell migration involves protrusion of the plasma membrane (lamellipodium extension) at the leading edge of the cell, which implies cytoskeleton reorganization such as actin [10] or vimentin, the major intermediate filament pro ...
MB_05_macWeb
... folding around them and forming a pouch. – The pouch then pinches off and becomes a membrane-bound organelle called a vesicle. ...
... folding around them and forming a pouch. – The pouch then pinches off and becomes a membrane-bound organelle called a vesicle. ...
Volume 7, (2002) pp 1137 – 1151 http://www.cmbl.org.pl Received
... The role of arabinogalactan in the transfer of intercellular signals can be also revealed using the Yariv’s reagent, which binds to arabinogalactan proteins [26]. The addition of this synthetic phenylglucoside to the medium blocks somatic embryogenesis, which was shown for example in suspension cult ...
... The role of arabinogalactan in the transfer of intercellular signals can be also revealed using the Yariv’s reagent, which binds to arabinogalactan proteins [26]. The addition of this synthetic phenylglucoside to the medium blocks somatic embryogenesis, which was shown for example in suspension cult ...
Skeletal Muscle Neural Cell Adhesion Molecule (N
... and Immunoblotting Two anti-N-CAM antibodies were used in the present study. These were the anti-N-CAM mAb H28 (15) and rabbit anti-mouse muscle N-CAM antisera. The latter reagent was produced as follows. Mouse G8-1 cells were grown to confluence and extracted with 1% NP-40 in Tris-buffered saline ( ...
... and Immunoblotting Two anti-N-CAM antibodies were used in the present study. These were the anti-N-CAM mAb H28 (15) and rabbit anti-mouse muscle N-CAM antisera. The latter reagent was produced as follows. Mouse G8-1 cells were grown to confluence and extracted with 1% NP-40 in Tris-buffered saline ( ...
- UCL Discovery
... remain on the perimeter membrane of the MVB from where they are recycled. When all the recycling proteins have been removed, the mature MVB can fuse with the lysosome and the contents are degraded (Futter et al., 1996). In more recent years, it has become clear that lysosomal fusion is not the only ...
... remain on the perimeter membrane of the MVB from where they are recycled. When all the recycling proteins have been removed, the mature MVB can fuse with the lysosome and the contents are degraded (Futter et al., 1996). In more recent years, it has become clear that lysosomal fusion is not the only ...
Rab-A2 and Rab-A3 GTPases Define a trans
... compartment even at 25 min or more after addition of the dye (cf. Figures 2A and 2B with Figure 2C and Supplemental Figures 7E and 7F online). Similarly, the GFP-BP80 PVC labeled slowly and partially with FM4-64 (see Supplemental Figures 8A to 8C online). In roots expressing both GFP-BP80 and YFP:RA ...
... compartment even at 25 min or more after addition of the dye (cf. Figures 2A and 2B with Figure 2C and Supplemental Figures 7E and 7F online). Similarly, the GFP-BP80 PVC labeled slowly and partially with FM4-64 (see Supplemental Figures 8A to 8C online). In roots expressing both GFP-BP80 and YFP:RA ...
Thesis - u
... companion cells to the sieve elements, they can also be part of large ribonucleoprotein phloem complexes. PP2s accumulate in the SE as protein bodies, partially associated to the filamentous phloem P-proteins [7]. We showed that PP2s bind to phloem sap proteins and are GlcNAc lectins [6]. In additio ...
... companion cells to the sieve elements, they can also be part of large ribonucleoprotein phloem complexes. PP2s accumulate in the SE as protein bodies, partially associated to the filamentous phloem P-proteins [7]. We showed that PP2s bind to phloem sap proteins and are GlcNAc lectins [6]. In additio ...
Microfabricated Chambers as Force Sensors for Probing
... 2. Spin down 1 ml of an exponentially growing culture of fission yeast cells and concentrate to 50 ml typically (use a similar protocol as if you were imaging yeast cells between a normal glass slide and a cover glass). 3. Place a 1 ml drop of cell suspension on the PDMS slab and rapidly cover with ...
... 2. Spin down 1 ml of an exponentially growing culture of fission yeast cells and concentrate to 50 ml typically (use a similar protocol as if you were imaging yeast cells between a normal glass slide and a cover glass). 3. Place a 1 ml drop of cell suspension on the PDMS slab and rapidly cover with ...
The liver
... The liver is a metabolically active organ responsible for many vital life functions. The primary functions of the liver are: • Bile production and excretion • Excretion of bilirubin, cholesterol, hormones, and drugs ...
... The liver is a metabolically active organ responsible for many vital life functions. The primary functions of the liver are: • Bile production and excretion • Excretion of bilirubin, cholesterol, hormones, and drugs ...
BioScience®
... more fodder to demolish the anti-intellectual arguments about the complexity of cilia being a hindrance to their evolution by natural selection. There are a wide range of microtubule-containing cell surface extensions found among protists, and they perform multiple functions, such as organelle trans ...
... more fodder to demolish the anti-intellectual arguments about the complexity of cilia being a hindrance to their evolution by natural selection. There are a wide range of microtubule-containing cell surface extensions found among protists, and they perform multiple functions, such as organelle trans ...
Protein Import, Replication, and Inheritance of a Vestigial
... derstanding of mitochondrial evolution and of the minimal set of proteins required for the biogenesis and inheritance of an endosymbiosis-derived organelle (7–9). Giardia lamblia is a unicellular protozoan parasite of the small intestine in vertebrates and a leading cause of diarrheal disease worldw ...
... derstanding of mitochondrial evolution and of the minimal set of proteins required for the biogenesis and inheritance of an endosymbiosis-derived organelle (7–9). Giardia lamblia is a unicellular protozoan parasite of the small intestine in vertebrates and a leading cause of diarrheal disease worldw ...