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The Arabidopsis TONNEAU2 Gene Encodes a Putative Novel
The Arabidopsis TONNEAU2 Gene Encodes a Putative Novel

... in the mutant background. Note, for example, that (F) and (I) show images of the same cells taken 25 min apart: a spindle with a transverse metaphase plate is followed by a phragmoplast that appears to show erratic growth along a longitudinal plane. (A), (F), and (I) show stacks of five (A), seven ( ...
2 StainsInMicro
2 StainsInMicro

... Stains and the Prokaryotic Envelope Prokaryotes are difficult to see in wet mounts because they are translucent. Wet mounts of live cells are useful in observing motility (swimming), the bacteria lack contrast against the white background. While special types of light microscopy have been invented t ...
Localization of proteins involved in endocytosis at tubulobulbar
Localization of proteins involved in endocytosis at tubulobulbar

Detecting and characterizing specialized ribosomes translating
Detecting and characterizing specialized ribosomes translating

... It has long been known that ribosome architecture and the basic mechanism of protein synthesis are highly conserved in evolution. For this reason, ribosomes have been traditionally regarded as a static factory that passively carry out translation, while the mechanisms controlling translation have be ...
Transport
Transport

... Jun Min Jung ...
Bacteriophage Multiplication—Dependence on Host Physiology
Bacteriophage Multiplication—Dependence on Host Physiology

... This conclusion was substantiated by raising cell size (and hence the PSS) using certain experimental manipulations that do not affect PSS concentration [2]: (a) specific inhibition of cell division by Pn [15]; (b) delay in cell division by thymine limitation of thyA mutants [18]; (c) letting synchr ...
Review on G protein coupled receptors A Satish Chandra, M Rama
Review on G protein coupled receptors A Satish Chandra, M Rama

... behavior and physiology. This is the largest class of receptors, with several hundred GPCRs identified thus far. Examples are receptors for hormones such as calcitonin and luteinizing hormone or neurotransmitters such as serotonin and dopamine. G protein-coupled receptors can be involved in patholog ...
Identification  of  a  novel  human ... through  its  interaction  with  the ...
Identification of a novel human ... through its interaction with the ...

... The plasmid GRB14/pRcCMVF containing full length GRB14 cDNA (21) was digested with Hind III and Klenow treated to create blunt ends, and then digested with Bcl I to release three fragments of approximately 1.1, 4.2 and 1.7 kb. The 1.7 kb fragment was isolated and cloned into the Nde I (Klenow treate ...
Heat shock proteins: the search for functions.
Heat shock proteins: the search for functions.

... proteolytic digestion products of the bovine clathrin uncoating enzyme. Further evidence was obtained by Bill Welch at Cold Spring Harbor Laboratory who noted that HSP70-1ike proteins have an ATP-binding property and found identity between proteolytic digestion products of HSP70-1ike proteins and th ...
on the nature of the "non-saturable" migration of
on the nature of the "non-saturable" migration of

... t r a n s p o r t s y s t e m for ])-amino acids, or for D-alanine specifically, one n o t accessible to the L-isomers, its u p t a k e should r e t a i n a degree of c o n c e n t r a t i o n d e p e n d e n c e in t h e presence of IOO mM L-alanine. Fig. 2 shows v e r y n e a r l y the same rates ...
Smooth
Smooth

... attachment to the dense bodies that contain a-actinin, a Z-band protein in skeletal muscle. Thus, it is assumed that the dense bodies function as Z-lines. The ratio of thin to thick filaments is much higher in smooth muscle (~15:1) than in skeletal muscle (~6:1). Smooth muscle is rich in intermediat ...
5_Muscle
5_Muscle

... What molecule serves as the energy source for fermentation? Where does the muscle cell get these molecules? ...
Peroxiredoxin-controlled G-CSF signalling at the endoplasmic
Peroxiredoxin-controlled G-CSF signalling at the endoplasmic

... of H2O2 for the so-called oxidative burst, a major weapon in host defence against bacteria. In non-phagocytic cells, H2O2 has long been considered as an unwanted by-product of cell metabolism, potentially hazardous because of the damaging effects on proteins, lipids, carbohydrates and nucleic acids ...
Epithelia PowerPoint Text
Epithelia PowerPoint Text

... lining, i.e. ions must flow from lumen into epithelial cells. •Thus, cell membranes of these epithelia contain various ion pumps that can allow ions to move in either direction - active transport systems that require ATP to function. Kidney - glomerulus and convoluted tubules ...
Condition-Dependent Cell Volume and Concentration of
Condition-Dependent Cell Volume and Concentration of

... often studied model bacterium Escherichia coli this information is hardly available and furthermore, certain measures (e.g. cell volume) are also dependent on the growth condition. In this work, we have determined these basic data for E. coli cells when grown in 22 different conditions so that respe ...
Human red blood cells-1 - Science Publishing Group
Human red blood cells-1 - Science Publishing Group

... Aging of the red blood cell revealed a decrease of cholesterol-to-phospholipids moral ratio , followed by a marked decrease in the activities of the membrane-bound enzymes, Na+,K+-ATPase [E.C.3.6.1.7] which is the marker of erythroid differentiation [30], is inactive in the intact membrane and is ac ...
Ion homeostasis, channels, and transporters: an update on cellular
Ion homeostasis, channels, and transporters: an update on cellular

... Na⫹ accumulation, K⫹ loss, and swelling that can culminate in lysis (2, 15). Early studies by Habermann et al. (15) revealed that these effects of PTX on erythrocyte ion homeostasis were substantially attenuated by the presence of ouabain, the classical and highly selective inhibitor of the Na⫹-K⫹-A ...
13059_2010_2529_moesm2_esm
13059_2010_2529_moesm2_esm

... glycosyltransferases (GTs) in T. atroviride and T. virens, they are close to T. reesei (103) and slightly above the average of the analysed fungi (95). The similar numbers of GTs that are found in different fungi are a probable consequence of the conserved role of the vast majority of GTs in houseke ...
The role of c-myc in cellular growth control
The role of c-myc in cellular growth control

... accounts for much of this regulation (Perry and Meyuhas, 1990). Although this mechanism is thought to be the main regulator of ribosomal protein content in mammalian cells, several well characterized transcription factors can transactivate ribosomal protein promoters. The most prominent transcriptio ...
T-cell regulator RNF125/TRAC-1 belongs to a novel family of
T-cell regulator RNF125/TRAC-1 belongs to a novel family of

... isolation. COS-7, HeLa and HEK-293T (human embryonic kidney) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) (Cambrex), 10 % (v/v) FCS, 100 units/ml penicillin and 0.1 mg/ml streptomycin. For transfection, 107 Jurkat T-cells were electroporated in 250 µl of RPMI 1640 medium containing ...
Abdominal Wall Defect Associated with Persistent Cloaca
Abdominal Wall Defect Associated with Persistent Cloaca

... according to the above principle, the type of AWD can be recognized. In this article, three cases of AWD in combination with an adjacent cloacal structure are presented. The probable derailments of embryogenesis are discussed, thereby elucidating the interrelation of the malformations ...
Molecular Architecture of the Exocyst Complex and its
Molecular Architecture of the Exocyst Complex and its

Passive transport
Passive transport

... site of the unloaded transporter on the extracellular side. This may seem contradictory to the requirement for a small K2 , except that the transporter is oriented in a membrane and so need not be symmetric. Binding glucose can cause conformational changes that change the bias. ...
Connective Tissue
Connective Tissue

... Some of these enzymes, e.g. collagenase, include a zinc atom & require Ca2+ to work - hence Matrix Metalloproteinases, e.g. MMP-3 The inhibitors of these enzymes go under the abbreviation TIMPs - Tissue Inhibitors of MMPs; & are also made by fibroblasts & other matrix-influencing cells ECM MOLECULA ...
Chlamydia
Chlamydia

... There appears to be no significant difference in morphology or developmental cycle among the various chlamydia, and a single generalized description applies to all. The development cycle may be regarded as an orderly alternation of the small and large cell type. It is initiated by the highly infecti ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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