pteridophyta - Institutul de Biologie
... For electron microscopy studies the mesophyll cells of the leaves from both in vitro culture as well as from native plants were used. These were processed by a conventional method (Mascorro and Bozola, 2007; Kuo, 2007): pre fixation in a solution of 3% glutaraldehyde in Na cacodylate buffer at 0.2M ...
... For electron microscopy studies the mesophyll cells of the leaves from both in vitro culture as well as from native plants were used. These were processed by a conventional method (Mascorro and Bozola, 2007; Kuo, 2007): pre fixation in a solution of 3% glutaraldehyde in Na cacodylate buffer at 0.2M ...
Axon and dendritic trafficking
... of vertebrate neurons demonstrated that SVs are highly motile organelles, interchangeable between many presynapses instead of being restricted to only a specific release site [15–17]. SVs form so-called superpools of vesicles spanning many presynaptic sites, which might provide a neuron with a versa ...
... of vertebrate neurons demonstrated that SVs are highly motile organelles, interchangeable between many presynapses instead of being restricted to only a specific release site [15–17]. SVs form so-called superpools of vesicles spanning many presynaptic sites, which might provide a neuron with a versa ...
Brachmann et al., 2005 Mol Cell Biol. 25, 2593
... Parallels between low molecular weight G protein (Ras, Rac Rho) regulation and heterotrimeric G protein regulation GDP/GTP Exchange Factor (GEF) activate: analogous to GPCR ...
... Parallels between low molecular weight G protein (Ras, Rac Rho) regulation and heterotrimeric G protein regulation GDP/GTP Exchange Factor (GEF) activate: analogous to GPCR ...
SICKLE CELL ANEMIA
... • Glutamate Valine at 6th position b globin • Sickle Hb forms polymers when deoxygenated • Polymerized sickle Hb injures RBC membrane and distorts its shape • Distorted RBC is hemolyzed ...
... • Glutamate Valine at 6th position b globin • Sickle Hb forms polymers when deoxygenated • Polymerized sickle Hb injures RBC membrane and distorts its shape • Distorted RBC is hemolyzed ...
A Role for Mitochondria in the Establishment and
... (Fig. 1D). As in the QC, mitochondria in the PM encircle the nucleus (Fig. 1E). However, in contrast to QC mitochondria, PM mitochondria frequently assumed a dumbbell shape (in approximately two-thirds [eight] of the roots examined ultrastructurally), and this is interpreted as indicative of their c ...
... (Fig. 1D). As in the QC, mitochondria in the PM encircle the nucleus (Fig. 1E). However, in contrast to QC mitochondria, PM mitochondria frequently assumed a dumbbell shape (in approximately two-thirds [eight] of the roots examined ultrastructurally), and this is interpreted as indicative of their c ...
KPC Notes
... Muscles perform three functions. Along with the skeleton they give support and structure to the body. When they contract, they account for movement of both the limbs and internal organs. Contraction also releases heat that serves to warm the body. Vertebrates have three types of muscle: Smooth muscl ...
... Muscles perform three functions. Along with the skeleton they give support and structure to the body. When they contract, they account for movement of both the limbs and internal organs. Contraction also releases heat that serves to warm the body. Vertebrates have three types of muscle: Smooth muscl ...
(6R)-hydroxy-BFA in tobacco and Arabidopsis
... the release of the TGN continues. Since BFA prevents vesiculation (presumably of clathrin-coated vesicles) at the TGN, the released malfunctioning TGNs accumulate to form a ‘BFA-compartment’. This can easily be demonstrated with any bona fide fluorescent TGN marker, for example, SYP61 (Fig. 1a, b). ...
... the release of the TGN continues. Since BFA prevents vesiculation (presumably of clathrin-coated vesicles) at the TGN, the released malfunctioning TGNs accumulate to form a ‘BFA-compartment’. This can easily be demonstrated with any bona fide fluorescent TGN marker, for example, SYP61 (Fig. 1a, b). ...
A theoretical analysis of the ephaptic feedback mechanism
... by an ephaptic mechanism should be divided by the number of ribbons and in that way reduce the effectiveness of ephaptic feedback. The essential point in this discussion is whether the potential distribution within the extracellular space of the cone pedicle will affect the feedback responses. Dmitr ...
... by an ephaptic mechanism should be divided by the number of ribbons and in that way reduce the effectiveness of ephaptic feedback. The essential point in this discussion is whether the potential distribution within the extracellular space of the cone pedicle will affect the feedback responses. Dmitr ...
Plant mitochondria contain the protein translocase subunits TatB
... believe that At5g43680 is a TatB like protein. This is also supported by basic sequence comparisons as At5g43680 shows a 14% identity and 29% similarity to EcTatB in comparison to 10% identity and 19% similarity to EcTatA. Furthermore, a sequence alignment between EcTatB and At5g43680 showed that it ...
... believe that At5g43680 is a TatB like protein. This is also supported by basic sequence comparisons as At5g43680 shows a 14% identity and 29% similarity to EcTatB in comparison to 10% identity and 19% similarity to EcTatA. Furthermore, a sequence alignment between EcTatB and At5g43680 showed that it ...
Fission yeast meu14+ is required for proper nuclear division and
... meu14+ fragment harboring the NdeI and NotI sites in the N- and Ctermini, respectively. After performing a TA-cloning procedure, this DNA fragment was digested by NdeI and NotI and inserted into the pRGT1 vector (a gift from M. Yamamoto, University of Tokyo), which was designed to fuse GFP to the in ...
... meu14+ fragment harboring the NdeI and NotI sites in the N- and Ctermini, respectively. After performing a TA-cloning procedure, this DNA fragment was digested by NdeI and NotI and inserted into the pRGT1 vector (a gift from M. Yamamoto, University of Tokyo), which was designed to fuse GFP to the in ...
Plant PCD In vegetative development Suspensor degradation
... 1. Mitochondria: ROS, DNA damage, other 2. Misfolded proteins 3. Dysfunctional stem cells ...
... 1. Mitochondria: ROS, DNA damage, other 2. Misfolded proteins 3. Dysfunctional stem cells ...
Tetherin Inhibits HIV-1 Release by Directly Tethering Virions to Cells
... maturation, we used a CHO-derived cell line lacking PIGL, an ER-resident enzyme required for the addition of GPI anchors. Proteins that harbor a GPI modification signal as their only membrane anchor are inserted into ER membrane but remain trapped there in the absence of PIGL (Nakamura et al., 1997) ...
... maturation, we used a CHO-derived cell line lacking PIGL, an ER-resident enzyme required for the addition of GPI anchors. Proteins that harbor a GPI modification signal as their only membrane anchor are inserted into ER membrane but remain trapped there in the absence of PIGL (Nakamura et al., 1997) ...
The role of cell cycle–regulated expression in the
... cells (Fig. 1 B). The signal at the proximal pole was still detectable in 29% (n 204) of daughter cells during the first budding cycle (Fig. 1 B, *), but it was only rarely detected (1%) in cells budding for the second or third time. In contrast to our previous observations (made using GFP– BUD9 ...
... cells (Fig. 1 B). The signal at the proximal pole was still detectable in 29% (n 204) of daughter cells during the first budding cycle (Fig. 1 B, *), but it was only rarely detected (1%) in cells budding for the second or third time. In contrast to our previous observations (made using GFP– BUD9 ...
Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of
... KAT1 plays an important role in guard cell functioning. We therefore analysed turgid guard cells transiently expressing KAT1 fused to green fluorescent protein (GFP). KAT1::GFP was organised in clusters in the PM similar to what we previously described for guard cell protoplasts (Hurst et al., 2004). ...
... KAT1 plays an important role in guard cell functioning. We therefore analysed turgid guard cells transiently expressing KAT1 fused to green fluorescent protein (GFP). KAT1::GFP was organised in clusters in the PM similar to what we previously described for guard cell protoplasts (Hurst et al., 2004). ...
In-Cell Solid-State NMR - Institut de Biologie Physico
... of samples can qualify as in cell: living cells (in vivo NMR), intact cells, and intact cellular compartments such as organelles and cellular envelopes (in situ NMR). Biological cells were studied by NMR as early as 1955 (1), and 15 years later the focus shifted from looking at water molecules to ex ...
... of samples can qualify as in cell: living cells (in vivo NMR), intact cells, and intact cellular compartments such as organelles and cellular envelopes (in situ NMR). Biological cells were studied by NMR as early as 1955 (1), and 15 years later the focus shifted from looking at water molecules to ex ...
The Biotechnology Century and Its Workforce
... The Plasma Membrane Selective permeability allows passage of some molecules Simple diffusion Facilitative diffusion Osmosis Active transport Endocytosis Phagocytosis: Pseudopods extend and engulf particles Pinocytosis: Membrane folds inward, bringing in fluid and dissolved substance ...
... The Plasma Membrane Selective permeability allows passage of some molecules Simple diffusion Facilitative diffusion Osmosis Active transport Endocytosis Phagocytosis: Pseudopods extend and engulf particles Pinocytosis: Membrane folds inward, bringing in fluid and dissolved substance ...
6_Acute Inflammation - V14-Study
... B) Secondary granules- smaller, less electron dense and more numerous. They contain lysosomes, lactoferrin, collagenase and others. -These are lethal enzymes that can kill bacteria or damaged the host tissue. 8) Understand Oxygen dependent and independent killing mechanisms: (p.14) Oxygen DependentA ...
... B) Secondary granules- smaller, less electron dense and more numerous. They contain lysosomes, lactoferrin, collagenase and others. -These are lethal enzymes that can kill bacteria or damaged the host tissue. 8) Understand Oxygen dependent and independent killing mechanisms: (p.14) Oxygen DependentA ...
Rheumatic patient has such symptom as destruction of cartilage
... 14. During postsynthetic period (G2) of cell cycle the synthesis of tubulin which takes part in the formation of mitotic spindle was disturbed. It can cause disorder of: a) Chromosome separation b) duration of mitosis c) Chromosome despiralization d) Chromosome spiralization e) Cytokinesis ...
... 14. During postsynthetic period (G2) of cell cycle the synthesis of tubulin which takes part in the formation of mitotic spindle was disturbed. It can cause disorder of: a) Chromosome separation b) duration of mitosis c) Chromosome despiralization d) Chromosome spiralization e) Cytokinesis ...
Michael P. Kowalski1, Vipat Raksakulthai2
... Drug developers are rapidly adopting three-dimensional (3D) cell cultures for drug screening since they provide a more physiologically relevant environment than two-dimensional cell cultures to screen compounds. Spheroids are a simple and well characterized, in vitro tumor model system and when deri ...
... Drug developers are rapidly adopting three-dimensional (3D) cell cultures for drug screening since they provide a more physiologically relevant environment than two-dimensional cell cultures to screen compounds. Spheroids are a simple and well characterized, in vitro tumor model system and when deri ...
phosphatases and differentiation of the golgi apparatus
... Goldfischer, 1961; Goldfischer et al. 1964 and others) in the root tip of Zea mays where morphological sequences of differentiation of the Golgi apparatus for participation in secretory activity have been established. The essentially linear arrangement of cells in this system has allowed close corre ...
... Goldfischer, 1961; Goldfischer et al. 1964 and others) in the root tip of Zea mays where morphological sequences of differentiation of the Golgi apparatus for participation in secretory activity have been established. The essentially linear arrangement of cells in this system has allowed close corre ...