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Collagen Binding Proteins Derived from the Embryonic Fibroblast
Collagen Binding Proteins Derived from the Embryonic Fibroblast

... To test the effects of synthetic peptides on the binding of CBP to collagen, octylglucoside extracts of 125I-surface labeled whole cells were applied to 200/zl columns of Type I collagen-Sepharose, fibronectin-Sepharose or albuminSepharose as described above. After washing to remove unbound material ...
A_P Unit 8 Study Guide New - Kenwood Academy High School
A_P Unit 8 Study Guide New - Kenwood Academy High School

... What are the survival needs and life functions of the lymphatic system? (1 MC) What is the anatomy and physiology of the lymphatic system? (2 MC) How do the lymphatic vessels and lymph nodes work? Where do the lymphatic vessels drain? (1 MC) What is the difference between a pathogen, antigen, and an ...
Microalgae Synthesize Hydrocarbons from Long
Microalgae Synthesize Hydrocarbons from Long

... variabilis NC64A. The solvent extracts of the saponification mixtures were analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The chromatograms of C. variabilis and C. reinhardtii extracts showed a peak at 14.3 min in the region of long-chain hydrocarbons (Fig. 1A). This peak displa ...
CYTOKINESIS IN HIGHER PLANTS
CYTOKINESIS IN HIGHER PLANTS

... (62, 63, 79). Female and male meiotic cells as well as microspores each undergo their own modes of cytokinesis, which are also distinct from embryo sac cellularization (61). However, cytokinesis of male meiotic cells in Arabidopsis was recently shown to resemble somatic cytokinesis in one important ...
Tackling Unwanted Proteolysis in Plant Production Hosts Used for
Tackling Unwanted Proteolysis in Plant Production Hosts Used for

... suitable strategies for the stabilization of recombinant proteins. Mass spectrometry based secretome analysis of tobacco BY-2 spent culture medium (Navarre et al., 2012), hydroponic culture medium of tobacco plants (Madeira et al., 2016; Wendlandt et al., 2016) and intercellular washing fluid of N. ...
Intrusive growth of flax phloem fibers is of intercalary type
Intrusive growth of flax phloem fibers is of intercalary type

... deduced from the increase in length of region 1, being 5.5 mm per day. The inset shows the upper part of the flax stem with marking of the stem regions. b Schematic representation of the growing flax stem tip with 10 leaf primordia (o). Distances between successive leaves on section are depicted in ...
Pulmonary Pathology, Pharmacology, and Pathophysiology
Pulmonary Pathology, Pharmacology, and Pathophysiology

... catheter is placed, measuring the pulmonary capillary pressure as normal. There is no history of cardiac disease, and the now cancelled surgery was for a routine gallbladder resection. What is the likely diagnosis? (2 points) A) Congestive heart failure B) Volume overload C) Transfusion related acut ...
Gozo, Malta - Gozo College Boys` Secondary School
Gozo, Malta - Gozo College Boys` Secondary School

... The disease would spare no nation, race or income group. There would be no certain way to avoid the pandemic. The strain A(H1N1) does not yet pass readily from one person to another. But the virus is evolving, and some of the affected avian species have now begun their winter migrations. As a sense ...
Acidotropic probes and flow cytometry: a powerful combination for
Acidotropic probes and flow cytometry: a powerful combination for

... with a 200 μm aperture nozzle, and dual excitation: 488 nm (blue laser) and 360 nm (UV laser). The cells were gated based on their size, shape and cell structural complexity using forward scatter (FSC) and side scatter (SSC) light. In addition, 3 photomultipliers measured cell autofluorescence: Gree ...
Dendritic cells express tight junction proteins and penetrate gut
Dendritic cells express tight junction proteins and penetrate gut

... Ligated loops were infected with (a,b,e) E. coli DH5α (109) expressing the red fluorescence protein (D5) or (c,d,f) a combination of 5×108 D5 + 5×108 S. typhimurium (SL) for 30 or 60 min. Intestines were then isolated and snap frozen. (a–d) Cryosections (7 µm) were fixed with 1% PFA and immunostaine ...
Chapter 01 FlexArt
Chapter 01 FlexArt

... (a) Elastic arteries. The tunica media is mostly elastic connective tissue. Elastic arteries recoil when stretched, which prevents blood pressure from falling rapidly. ...
1 Final Examination – Pulmonary Pathology, Pharmacology, and
1 Final Examination – Pulmonary Pathology, Pharmacology, and

... catheter is placed, measuring the pulmonary capillary pressure as normal. There is no history of cardiac disease, and the now cancelled surgery was for a routine gallbladder resection. What is the likely diagnosis? (2 points) A) Congestive heart failure B) Volume overload C) Transfusion related acut ...
the kidneys - ETRA
the kidneys - ETRA

... • It usually starts with vesicular skin rash and becomes itchy with raw pockmarks. • It is spread through coughs or Chicken pox usually starts with vesicular skin sneezes of ill individuals or rash mainly on the body head rather than at through direct contact with and the periphery. It gives a ...
Ciliary neurotrophic factor maintains the pluripotentiality of
Ciliary neurotrophic factor maintains the pluripotentiality of

... restricted expression is apparently responsible for limiting CNTF to its actions on neuronal and glial cells (Davis et al., 1991; Ip et al., 1993). The finding that the CNTF receptor complex includes a functional LIF receptor raised the possibility that some cells previously found to be LIFresponsiv ...
Structure and Nanostructure of the Outer Tangential Epidermal Cell
Structure and Nanostructure of the Outer Tangential Epidermal Cell

... (Freeman et al., 1979a) and Vaccinium ashei. Rodlet structure waxes in blueberries were due to the presence of -diketones (Freeman et al., 1979b). Von WettsteinKnowles (1974) reported that the loss of rodlet structure waxes was coincidental with the decrease in -diketones. This author established ...
Atrophic Vaginitis
Atrophic Vaginitis

... type 2 testing. HSV 2 (herpes simplex virus) may be latent and/or asymptomatic for decades, activating in perimenopause or menopause. Genital HSV 2 is very common, and prevalence increases with age, affecting 1 out of 3 women over 30 years of age. Adding to the diagnostic challenge most clinical pre ...
1 Cell polarity and asymmetric cell division: the C. elegans early
1 Cell polarity and asymmetric cell division: the C. elegans early

... domains which in turn determine the leading edge during migration, confer the directionality of transport and/or secretion of molecules within the tissue, or ensure the distribution of cell-fate determinants. Thus cell polarity is essential for the function of almost all cell types and, when perturb ...
Human pancreas development - The Company of Biologists
Human pancreas development - The Company of Biologists

... 8 weeks of development prior to the fetal period. Counting somite pairs by light microscopy becomes more difficult after CS11. AIP, anterior intestinal portal. *The estimation of mouse development relates broadly to the Carnegie stage, not the key event in human pancreas development. ...
Vacuole Membrane Protein 1 Is an Endoplasmic
Vacuole Membrane Protein 1 Is an Endoplasmic

... the exceptional ability to form a multicellular organism by aggregation of solitary cells. The differentiation program is triggered by starvation and leads to the formation of a fruiting body composed of spores supported by a stalk (Escalante and Vicente, 2000). In this report, we describe the first ...
Molecular characterization of carnitinedependent transport of
Molecular characterization of carnitinedependent transport of

... Based on these findings, we developed a selective screen for the isolation of mutants that are specifically defective in the carnitine-dependent acetyl-unit transport from peroxisomes to mitochondria (CDAT mutants). In this paper we report the isolation and characterization of various mutants, which ...
Supplementary Methods
Supplementary Methods

... demethylation at varying doses of 5-Aza-dC (0 – 8 M). Amplicon contains 30 CpG sites and differentially methylated sequences were resolved according to differences in melting temperature. Previously characterized KG1a (>95% methylated) and normal CD34 cells (0% methylated) served as controls for de ...
Cellular Maturation Defects in Bruton`s Tyrosine
Cellular Maturation Defects in Bruton`s Tyrosine

... induction of cell surface changes in Btk-deficient small pre-B cells is still noticeable at the immature B cell stage, in particular with regard to the expression levels of BP-1 and CD2 (5). In this respect, we now found that ␬ L chain-positive immature B cells are more affected by the lack of Btk t ...
BIO 100 coursepack FA2015
BIO 100 coursepack FA2015

... Tardies (up to 15 minutes late) count as ½ of an absence. If you arrive late on days in which quizzes or tests are administered, you will not receive additional time to complete the assessment. If you arrive after the quiz has been collected, you will not be offered an opportunity to take the quiz. ...
Cell biology # 2 - Nutley Public Schools
Cell biology # 2 - Nutley Public Schools

... 6 Fused vesicle may (a) fuse with lysosome for digestion of its contents, or (b) deliver its contents to the plasma membrane on the opposite side of the cell (transcytosis). © 2013 Pearson Education, Inc. ...
CYTOKINESIS IN HIGHER PLANTS
CYTOKINESIS IN HIGHER PLANTS

... (62, 63, 79). Female and male meiotic cells as well as microspores each undergo their own modes of cytokinesis, which are also distinct from embryo sac cellularization (61). However, cytokinesis of male meiotic cells in Arabidopsis was recently shown to resemble somatic cytokinesis in one important ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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