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Functional Genomics Screening with Invitrogen™ LentiArray
Functional Genomics Screening with Invitrogen™ LentiArray

Human Vascular Smooth Muscle Cells Contain Functional Estrogen
Human Vascular Smooth Muscle Cells Contain Functional Estrogen

... estrogenic effects of this compound.37 Cells were maintained in DMEM containing 10% fetal bovine serum in which the E2 content was determined to be less than 2.6x10-11 mol/L (Hyclone). VSMCs were allowed to migrate from the primary explants and were subsequently passaged at confluence. Cultured cell ...
Text S1.
Text S1.

... minC as a reporter gene Prior to engineering the nanorecorder’s genetic circuit, we evaluated four different E. coli host cell strains (DH5α, GM2929, CSH50 and PB103) for their endogenous cell lengths. Of these, only PB103 host cells displayed a cell population with minimal variation in cellular len ...
Phosphorylation of initiation factor2 is required for
Phosphorylation of initiation factor2 is required for

A herpes simplex virus type 1 mutant deleted for 34.5 and
A herpes simplex virus type 1 mutant deleted for 34.5 and

... days using a nylon -tipped swab to look for spontaneously reactivated virus as previously described.12 The swab was then placed in 0.5 mL tissue culture medium, squeezed, and the inoculated medium was used to infect monolayers of RS cells. These cell monolayers were observed in a masked fashion by p ...
Phenotypic Modulation of Smooth Muscle Cells
Phenotypic Modulation of Smooth Muscle Cells

... (CC(A/T)6GG), which serves as the binding site for serum response factor (SRF), a MADS (MCM1, Agamous, Deficiens, SRF) box transcription factor (reviewed in Miano, 2003). Depending on intracellular signals and cell type, proteins containing the MADS domain are capable of activating or repressing dis ...
Data Sheet
Data Sheet

... L-type amino acid transporter 1 (LAT1) is a predicted 12 membrane-spanning protein and is unique because it requires an additional single membrane spanning protein, 4F2 heavy chain (4F2hc:CD98), for its functional expression. L-type is Na+ -independent neutral amino acid transporter agency and essen ...
DNA Microarrays Slides - URMC
DNA Microarrays Slides - URMC

... calls in repair enzymes and triggers apoptosis (cell death) if DNA damage cannot be repaired ...
Isolation and Quantitation of HIV in Peripheral
Isolation and Quantitation of HIV in Peripheral

... nucleus surrounded by a single nuclear membrane and a translucent “bubble” (which is the consequence of the fusion of the cytoplasms) are usually visible either at the cellular pole opposite to the site where the nuclei are clustered (Fig. 12.2.1A) or surrounding the nuclei (Fig. 12.2.1B). “Old” sy ...
Evolution and Development
Evolution and Development

... It has long been held that the neural crest is a defining feature of vertebrates. The neural crest arises at the dorsal aspect of the neural tube and then migrates widely in the embryo, giving rise to a range of derivatives which are distinctly vertebrate, such as the neurons and glia of the periphe ...
F214: Communication, Homeostasis and Energy 4.2.1 The Kidney
F214: Communication, Homeostasis and Energy 4.2.1 The Kidney

... acids by facilitated diffusion As concentration rises, they are able to diffuse out of the opposite side of the cell into the tissue fluid- active transport may also support this process from the tissue fluid, they diffuse into the blood and are ...
Activity of isatizon according to adenoviral infection in vitro
Activity of isatizon according to adenoviral infection in vitro

KEY TO CELL WORKSHEET
KEY TO CELL WORKSHEET

... • These protein filaments along with microfilaments and intermediate filaments compose the CYTOSKELETON of the cell. • These function to support the cell and are sometimes called the “BONES and MUSCLES” of the cell. • These protein filaments also allow for ...
histo-anatomical aspects of the nepenthes max7m4 reinw. ex ness
histo-anatomical aspects of the nepenthes max7m4 reinw. ex ness

... Under the upper epidermis, 3-4 layers of large, isodiametrical cells (aquiferous cells) could be observed. The palisade parenchyma is located under this aquiferous tissue and is formed by 2-3 layers of short cells. The spongy parenchyma is thick, formed by rounded cells with small aeriferous spaces ...
Reversible Injury
Reversible Injury

... Dilation of Endoplasmic reticulum Nuclear Alteration ...
Downloaded - The Journal of Immunology
Downloaded - The Journal of Immunology

... Cellular immune responses are crucial for the control of EBV-associated lymphoproliferative diseases. To induce an anti-EBV cell-mediated immunity, we have used dendritic cells (DCs) generated by a 3-day culture of human CD14ⴙ monocytes in the presence of GM-CSF and type I IFN (IFN-DCs) and pulsed w ...
Cytoskeleton remodelling of confluent epithelial cells cultured on
Cytoskeleton remodelling of confluent epithelial cells cultured on

... and cell–cell contact formation. Cellular adhesion determines the function and fate of eukaryotic cells to a much larger extent than initially expected. In particular, environmental cues such as those emanating from the substrate itself, like topography, elasticity or surface functionalization, gove ...
CHAPTER 21 VIRUSES, BACTERIA AND ARCHAEA
CHAPTER 21 VIRUSES, BACTERIA AND ARCHAEA

... 3. Viruses often mutate; therefore, it is correct to say that they ________________. a. Those that mutate are troublesome; a vaccine ________________today may not be effective ________________. b. Influenza (flu) viruses ________________regularly. C. Viral Reproduction 1. Viruses gain entry into and ...
CHAPTER 4 HISTOLOGY: THE STUDY OF TISSUES
CHAPTER 4 HISTOLOGY: THE STUDY OF TISSUES

... Have students design tissues to fit certain structural criteria. Ask how structure relates the to function of the new tissues. For example, one group of students can determine the functional characteristics of a connective tissue with only proteoglycan or hydroxyapatite in the matrix. Would this typ ...
What the Distribution of Cell Lengths in the Root Meristem Does and
What the Distribution of Cell Lengths in the Root Meristem Does and

... 2000), the constraint of symplastic growth offers a simple way to see if dividing cells exit the cell cycle while still within the meristem. Because of symplastic growth, a cell that misses a mitosis will become larger than its neighbors. It is true that single cells, such as protozoa or cells in cu ...
pSUPER.retro.puro : Manual A Vector System for Expression of
pSUPER.retro.puro : Manual A Vector System for Expression of

Glossary
Glossary

... A mutation that makes a gene in an organism inoperative or unexpressed; e.g. a “leptin receptor knockout mouse” does not express any functional leptin receptor. Kinetochore: A protein structure located at the centromere of a chromosome. The kinetochore is the site of spindle fibre attachment (Lectur ...
5 Organelles
5 Organelles

... entering (osmosis) the vacuole, which then swells exerting internal force on the cell wall •Causes “rigidity” so the plant my increase by stacking cells ...
Cell Biology Core
Cell Biology Core

... Cell Biology Core •Because the drag is the same for diffusion as for externally applied forces, the diffusion coefficient can be derived ...
Cell Boundaries - Duplin County Schools
Cell Boundaries - Duplin County Schools

... • As water diffuses toward the hypertonic side of a membrane, this produces OSMOTIC PRESSURE acting on the hypertonic side of the membrane • Cells have salts, sugars, proteins, and other solutes dissolved in the cytoplasm, making the inside of cells hypertonic to fresh/distilled water; if not checke ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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