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Determination of DNA replication kinetics in synchronized human
Determination of DNA replication kinetics in synchronized human

... The PCR-based assay to determine the replication time of single copy DNA sequences is schematically illustrated in Fig. 1. An asynchronously growing cell population is reversibly arrested at the Gl/S boundary using the DNA synthesis inhibitor CPX (12) to achieve an efficient synchronous entry into t ...
Different involvement of the mitochondrial, plastidial and
Different involvement of the mitochondrial, plastidial and

... (35C) does not affect cell viability, whereas cell exposure to 55C HS induces programmed cell death (PCD). In relation to ASC-GSH cycle, the three analysed compartments have specific enzymatic profiles that are diversely altered by the HS treatments. The cytosol contains the highest activity of al ...
Morphology & Cell Biology of Bacteria (Part II)
Morphology & Cell Biology of Bacteria (Part II)

... structures called organelles such as mitochondria and chloroplasts Eukaryotic cells can reproduce in one of several ways, including meiosis (sexual reproduction) and mitosis (cell division producing identical daughter cells). ...
Sagittula stellata gen. nov., sp. nov., a Lignin
Sagittula stellata gen. nov., sp. nov., a Lignin

... genus Roseobacter. A signature secondary structure of the 16s rRNA is proposed. The guanine-plus-cytosine content of the genomic DNA was 65.0 mol%. On the basis of the results of 16s rRNA sequence and phenotypic characterizations, the isolate was sufficiently different to consider it a member of a n ...
Concept 1.1 Introduction to the Sciences Lesson Essential Question
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... Cell Theory Lesson Essential Question(s): How did early studies led to the development of the cell theory? What characteristics do most cells share? Vocabulary: Cell, Cytoplasm, Eukaryotic cells (Eukaryote), Organelles, Prokaryotic cells (Prokaryote), Unicellular Concept 4.2 Cell Organelles Lesson E ...
Planctomyces stranskae - International Journal of Systematic and
Planctomyces stranskae - International Journal of Systematic and

... (see figures). Up to now, the existence of two similar, yet distinguishable, bulbiform, budding bacteria has not generally been realized. The aforementioned form, with a prominent spike at its globose end, is widely distributed and quite well known ( 5 , 7 , 8 , 11, 18). The other form, which lacks ...
Name
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The chromosome partitioning protein, ParB, is required for
The chromosome partitioning protein, ParB, is required for

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Polycystin-2 takes different routes to the somatic and ciliary plasma
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Inefficient response of T lymphocytes to
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Peer-reviewed Article PDF
Peer-reviewed Article PDF

... (Yp) non-mosaic, even though the isodicentric rearrangement of Y chromosome is frequent because of the palindromic sequences at the ends heterochromatic Yq12, site of recombination [15]. Moreover, this distal portion is full of fragile sites characterized by AT sequences interspaced by Alu repetitiv ...
Jeopardy
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Signalling in Plant Lateral Organ Development
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Cell shape changes during gastrulation in
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Structural Requirements of Simple Organic
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Targets of Oncogenes and Tumor Suppressors: Key for
Targets of Oncogenes and Tumor Suppressors: Key for

... desired gene (in the case of tumor suppressors); d) ability to cause morphological transformation and/or unlimited growth (oncogenes) or suppression of cell growth and/or manifestations of transformation (tumor suppressors) in cultivated cells in vitro. The last two decades are characterized by the ...
Involvement of the Vacuoles of the Endodermis in
Involvement of the Vacuoles of the Endodermis in

... @bs.aist-nara.ac.jp; fax 81-743-72-5489. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010216. ...
wingless acts through the shaggy/zeste-white 3
wingless acts through the shaggy/zeste-white 3

... f+71b). Embryos from this cross were collected for 24 hour periods, aged at 25°C and irradiated at 60 or 84 hours (±12 hours) after egg laying (corresponding to 1st and 2nd larval instars, taking into account the developmental delay caused by the Minute mutant). Irradiation was performed at 150 kV, ...
LESSON PLANNING CLINIC – WORKSHEET (for five
LESSON PLANNING CLINIC – WORKSHEET (for five

... about how multi-cellular organisms are different from single celled organisms. Plants and animals are some examples of multi-cellular organisms that are made up of many cells. These cells are specialized, meaning that joined together they perform separate functions for an organism to keep it alive. ...
Lysosomal Sequestration of Polyamine
Lysosomal Sequestration of Polyamine

... was found to be stable, they were cross-resistant to other known AdoMetDC inhibitors, and they were found to be less sensitive to DENSPM despite accumulating ~5-fold more of the analogue than the parental line (6). In this subsequent detailed characterization. CHO/664 (also designated CHO/100) cells ...
between TLRs and FcRs Polyfunctional Th Cells by Cross
between TLRs and FcRs Polyfunctional Th Cells by Cross

... Therefore, we investigated the initiation of immune responses after simultaneous recognition of Ab-opsonized pathogens by PRRs and FcRs on monocyte-derived immature DCs (iDCs), because DCs are the most efficient APCs and secrete a plethora of cytokines and lipid mediators that direct polarization of ...
Probes for Multiplexed Detection of GFP
Probes for Multiplexed Detection of GFP

... Molecular Probes offers a diverse array of cell-permeant fluorescent stains that selectively associate with the mitochondria, lysosomes, endoplasmic reticulum and Golgi apparatus in live cells. These probes, which include our exclusive MitoTracker, MitoFluor, LysoTracker, LysoSensor, RedoxSensor and ...
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10digestion2009print..

... This is where all the work is done! ...
Introduction The Doctrinal Neuron as a Functional Unit
Introduction The Doctrinal Neuron as a Functional Unit

... Years ago I placed my bet that they will be found in the brains of mammals, once we learn how to recognize them or to penetrate many cells with intracellular electrodes. That bet has not paid off yet, but it still holds. Even more common, I believe, will be found neurons that spike, but in between s ...
The Role of the Leukemia Inhibitory Factor (LIF)
The Role of the Leukemia Inhibitory Factor (LIF)

... early embryonic development can occur also in the absence of some components of the LIF-pathway but many of these mouse models then display later on during development or during adulthood many different disorders (Table 1). Lif−/− embryos, for instance, develop normally into adulthood [4,5] but even ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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