Protocol: Genome-scale CRISPR-Cas9 Knockout
... and efficiency33, 39. Although specificity and efficiency will likely vary across experimental settings, false positive sgRNAs in screens can still be mitigated by including redundant sgRNAs in the library and requiring multiple distinct sgRNAs targeting the same gene to display the same phenotype w ...
... and efficiency33, 39. Although specificity and efficiency will likely vary across experimental settings, false positive sgRNAs in screens can still be mitigated by including redundant sgRNAs in the library and requiring multiple distinct sgRNAs targeting the same gene to display the same phenotype w ...
File - Groby Bio Page
... The production of pigment in rabbit fur is controlled by two genes. One gene controls whether any pigment is made. This gene has three alleles. Allele A codes for the production of one form of the enzyme tyrosinase, which converts tyrosine into a black pigment. Allele Ah codes for the production of ...
... The production of pigment in rabbit fur is controlled by two genes. One gene controls whether any pigment is made. This gene has three alleles. Allele A codes for the production of one form of the enzyme tyrosinase, which converts tyrosine into a black pigment. Allele Ah codes for the production of ...
Leading The Way in Genetic Fertility Screening
... an intense focus of investigation, and miRNAs have been extensively profiled in many bodily fluids including amniotic and follicular fluids (Weber et al., 2010, Sang et al., 2013). Studies have demonstrated that secreted miRNAs have strong potential as useful prognostic metrics in clinical IVF setti ...
... an intense focus of investigation, and miRNAs have been extensively profiled in many bodily fluids including amniotic and follicular fluids (Weber et al., 2010, Sang et al., 2013). Studies have demonstrated that secreted miRNAs have strong potential as useful prognostic metrics in clinical IVF setti ...
University of Groningen Methionine
... elevated expression of various genes/operons involved in methionine synthesis and transport (fhs, folD, gshT, metA, metBcsd, metEF, metQ, tcyB, spd-0150, spd-0431 and spd-0618). Furthermore, b-galactosidase assays and quantitative RTPCR studies demonstrated that the transcriptional regulator, CmhR ( ...
... elevated expression of various genes/operons involved in methionine synthesis and transport (fhs, folD, gshT, metA, metBcsd, metEF, metQ, tcyB, spd-0150, spd-0431 and spd-0618). Furthermore, b-galactosidase assays and quantitative RTPCR studies demonstrated that the transcriptional regulator, CmhR ( ...
no correlation between cyp17-34t/c polymorphism and severe acne
... All subjects were examined consecutively in our outpatient unit by dermatologists. Patients with acne venenata and acne cosmetica were excluded from the study. The clinical grade of acne was assessed based on the Consensus Conference on Acne Classification (Gollnick et al., 2003). According to these ...
... All subjects were examined consecutively in our outpatient unit by dermatologists. Patients with acne venenata and acne cosmetica were excluded from the study. The clinical grade of acne was assessed based on the Consensus Conference on Acne Classification (Gollnick et al., 2003). According to these ...
ZytoLight ® CLL I Probe SPEC TP53/ATM Dual Color Probe
... LAMP1 (lysosome-associated membrane protein 1) gene region in 13q34. Due to cross-hybridizations of chromosome 13 alpha satellites to other centromeric regions, probes specific for 13q34 are frequently used for chromosome 13 copy number detection. ...
... LAMP1 (lysosome-associated membrane protein 1) gene region in 13q34. Due to cross-hybridizations of chromosome 13 alpha satellites to other centromeric regions, probes specific for 13q34 are frequently used for chromosome 13 copy number detection. ...
Exam Questions from Exam 1 – Basic Genetic Tests
... Next you isolate a mutant strain of yeast that cannot grow on medium lacking leucine. This strain contains a single mutation you call leu1–. The leu1– mutation is near to drk1– on the same chromosome. When the leu1– mutant is mated to wild-type yeast, the resulting diploids cannot grow on medium lac ...
... Next you isolate a mutant strain of yeast that cannot grow on medium lacking leucine. This strain contains a single mutation you call leu1–. The leu1– mutation is near to drk1– on the same chromosome. When the leu1– mutant is mated to wild-type yeast, the resulting diploids cannot grow on medium lac ...
Conserved syntenic clusters of protein coding genes are missing in birds
... comparative genomics analysis to identify orthologous gene sets in humans (Homo sapiens), a lizard (green anole; Anolis carolinensis) representing a non-avian sauropsid, a galliform, (chicken; Gallus gallus) representing a basal avian order with a high quality genome assembly, and an oscine passerif ...
... comparative genomics analysis to identify orthologous gene sets in humans (Homo sapiens), a lizard (green anole; Anolis carolinensis) representing a non-avian sauropsid, a galliform, (chicken; Gallus gallus) representing a basal avian order with a high quality genome assembly, and an oscine passerif ...
marker assisted selection in disease resistance breeding
... acceptable and that the pathogen reproduces by sexual means. Also, multilines may not hold a necessary uniformity that many cropping systems require, which make them not feasible to practical utility. The second option for durable resistance is gene pyramiding. In theory, pyramiding several “undefea ...
... acceptable and that the pathogen reproduces by sexual means. Also, multilines may not hold a necessary uniformity that many cropping systems require, which make them not feasible to practical utility. The second option for durable resistance is gene pyramiding. In theory, pyramiding several “undefea ...
Gibberellin Regulates Mitochondrial Pyruvate Dehydrogenase
... Treatment with GA3 reduced the mtPDH activity of the transgenic lines; however, interestingly, it still remained significantly higher, 30–40%, than the control (Fig. 5B). These results confirmed that changes in OsPDK1 expression at the transcript level did modulate mtPDH activity. Expression pattern ...
... Treatment with GA3 reduced the mtPDH activity of the transgenic lines; however, interestingly, it still remained significantly higher, 30–40%, than the control (Fig. 5B). These results confirmed that changes in OsPDK1 expression at the transcript level did modulate mtPDH activity. Expression pattern ...
DNA How the Molecule of Heredity Carries, Replicates, and
... DNA stores information in the sequence of its bases. •Much of DNA’s sequence-specific information is accessible only when the double helix is unwound. •Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. Proteins can either bind to a DNA sequence, or initiate the copying of it. ...
... DNA stores information in the sequence of its bases. •Much of DNA’s sequence-specific information is accessible only when the double helix is unwound. •Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. Proteins can either bind to a DNA sequence, or initiate the copying of it. ...
document
... http://videos.howstuffworks.com/sciencentral/2698-myostatin-andmusclegrowth-video.htm Genetic mutation turns tot into superboy - http://www.msnbc.msn.com/id/5278028/ ...
... http://videos.howstuffworks.com/sciencentral/2698-myostatin-andmusclegrowth-video.htm Genetic mutation turns tot into superboy - http://www.msnbc.msn.com/id/5278028/ ...
Benjamin Schlegel
... results of the present study do not support a role for these genes in BCA or JHS. A subset of unique genes identified in one bovine clostridial abomasitis isolate (F262), for which a genome sequence is available, was searched for in 8 BCA isolates by PCR. None of the 10 genes were consistently prese ...
... results of the present study do not support a role for these genes in BCA or JHS. A subset of unique genes identified in one bovine clostridial abomasitis isolate (F262), for which a genome sequence is available, was searched for in 8 BCA isolates by PCR. None of the 10 genes were consistently prese ...
Is HP1 an RNA detector that functions both in repression and
... intriguing implications for both how HP1 is recruited to chromosomes and how it might specify distinct activities at distinct sites. HP1 is now known to be a highly interactive protein; it is capable of interacting with a host of proteins with a range of nuclear activities (Kellum, 2003; and referen ...
... intriguing implications for both how HP1 is recruited to chromosomes and how it might specify distinct activities at distinct sites. HP1 is now known to be a highly interactive protein; it is capable of interacting with a host of proteins with a range of nuclear activities (Kellum, 2003; and referen ...
Cloning: A Critical Analysis of Myths and Media
... also used in genetic engineering efforts, such as transgenic technology (where DNA from one species is inserted into another) and/or gene pharming (where scientists alter an animal’s DNA so that it can make human proteins, drugs, or compounds that have medicinal or other applications). Transgenic an ...
... also used in genetic engineering efforts, such as transgenic technology (where DNA from one species is inserted into another) and/or gene pharming (where scientists alter an animal’s DNA so that it can make human proteins, drugs, or compounds that have medicinal or other applications). Transgenic an ...
Understanding Genetics and the Sire Summaries
... Predicted Transmitting Ability is an estimate of genetic superiority that a bull or cow will transmit to its offspring for a given trait. PTAs are calculated for milk pounds, fat pounds, fat percent, protein pounds, protein percent, somatic cell score, productive life, net merit and final type score ...
... Predicted Transmitting Ability is an estimate of genetic superiority that a bull or cow will transmit to its offspring for a given trait. PTAs are calculated for milk pounds, fat pounds, fat percent, protein pounds, protein percent, somatic cell score, productive life, net merit and final type score ...
Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos
... with insertion/deletion events were separated in some cases (ID = 4, 21, 22 and 24), but not in others (ID = 10 and ID = 12, with insertion /deletion of three nucleotides, were not separated). Table 1 also shows that even samples containing the alleles that were most similar in ...
... with insertion/deletion events were separated in some cases (ID = 4, 21, 22 and 24), but not in others (ID = 10 and ID = 12, with insertion /deletion of three nucleotides, were not separated). Table 1 also shows that even samples containing the alleles that were most similar in ...
Genetics of Male Infertility - the Infertility Center of St. Louis
... chromosome made it very difficult with standard STS markers to define smaller deletions (104). The unusually repetitive sequence structure of the AZFc region of the Y plagued even the first attempts at constructing a physical map with YAC’s, because repetitive STS’s could not be accurately placed in ...
... chromosome made it very difficult with standard STS markers to define smaller deletions (104). The unusually repetitive sequence structure of the AZFc region of the Y plagued even the first attempts at constructing a physical map with YAC’s, because repetitive STS’s could not be accurately placed in ...
Supplemental Material
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
Promega Notes: T4 RNA Ligase: A Molecular Tool for RNA and DNA
... Figure 1. Purity of Promega's T4 RNA Ligase and three other commercially available enzymes. Twenty units of RNA ligase from Vendors A, B, C and Promega were resolved on a 4-20% NOVEXTM polyacrylamide gel containing SDS and stained with Coomassie® brilliant blue. ...
... Figure 1. Purity of Promega's T4 RNA Ligase and three other commercially available enzymes. Twenty units of RNA ligase from Vendors A, B, C and Promega were resolved on a 4-20% NOVEXTM polyacrylamide gel containing SDS and stained with Coomassie® brilliant blue. ...
2- pcr primer design and reaction optimisation
... about 5oC below the lowest Tm of the pair of primers to be used (Innis and Gelfand, 1990). A more rigorous treatment of Ta is given by Rychlik et al. (1990): they maintain that if the Ta is increased by 1oC every other cycle, specificity of amplification and yield of products <1kb in length are both ...
... about 5oC below the lowest Tm of the pair of primers to be used (Innis and Gelfand, 1990). A more rigorous treatment of Ta is given by Rychlik et al. (1990): they maintain that if the Ta is increased by 1oC every other cycle, specificity of amplification and yield of products <1kb in length are both ...
4 Molecular Genetics of Coat Colour, Texture and Length in the Dog
... genetic system for efficient gene mapping. In what follows, we first describe the modern ‘casecontrol’ approach to mapping genetic traits in the dog in the context of population history. We then present an overview of coat colour genetics using the framework established by C.C. Little (Little, 1957) ...
... genetic system for efficient gene mapping. In what follows, we first describe the modern ‘casecontrol’ approach to mapping genetic traits in the dog in the context of population history. We then present an overview of coat colour genetics using the framework established by C.C. Little (Little, 1957) ...
Exome sequencing as a tool for Mendelian disease gene discovery
... than 2 years, exome sequencing has been used to identify causal alleles for several dozen Mendelian disorders 2,11,15–38 (Supplementary information 1 (table)). Most of these studies have, to varying extents, relied on comparisons with exome sequences and variants that are found in a small number of ...
... than 2 years, exome sequencing has been used to identify causal alleles for several dozen Mendelian disorders 2,11,15–38 (Supplementary information 1 (table)). Most of these studies have, to varying extents, relied on comparisons with exome sequences and variants that are found in a small number of ...
Application of whole genome sequencing to fully characterise
... Using PacBio sequencing, we were able to add a further 17 high quality reference genomes to the general database, eleven of which (ten C. jejuni and one C. coli) assembled as a single chromosome. From 14 high quality PacBio genomes and three previously available complete genomes, we defined a C. jej ...
... Using PacBio sequencing, we were able to add a further 17 high quality reference genomes to the general database, eleven of which (ten C. jejuni and one C. coli) assembled as a single chromosome. From 14 high quality PacBio genomes and three previously available complete genomes, we defined a C. jej ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse