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101 -- 2006
101 -- 2006

... a) initiation, termination, elongation. d) elongation, initiation, termination. b) elongation, termination, initiation. e) initiation, elongation, termination. c) termination, elongation, initiation. __ 39. In which directions does transcription occur? a) in the 3’ to 5’ direction c) in the 3’ to 3’ ...
2-Slides
2-Slides

... Originally only one sex (asexual reproduction … cloning_..) mitosis ~ complete inheritance > 1 sex ? ...
Genes, Chromosomes and DNA
Genes, Chromosomes and DNA

... • Each new double helix is composed of an old (parental) strand and a new (daughter) strand. • As each strand acts as a template, process is called Semi-conservative Replication. • Replication errors can occur. Cell has repair enzymes that usually fix problem. An error that persists is a mutation. • ...
LN #18 Heredity
LN #18 Heredity

... organism looks like. • In order to determine an organisms phenotype you need to look at it. ...
Chromatin structure - U of L Class Index
Chromatin structure - U of L Class Index

... One out of 100 nucleotides bears and added methyl group, which is always attached to carbon 5 of cytosine in the 5’-CG-3’ rich island that are often located in or near transcriptional regulatory regions. DNA methylation serves more to maintain a gene in an inactive state than as a mechanism for init ...
p AB - UCL
p AB - UCL

... Evolution may affect frequencies of two- and more locus associations, as well as just gene frequencies. Deviation from 2-locus equilibrium is known as gametic disequilibrium or linkage disequilibrium, measured by D D is destroyed by recombination, c, so Dt = D0(1 -c)t D can increased by selection, m ...
X-Linked Recessive Inheritance
X-Linked Recessive Inheritance

... Linked Genes  Genes carried on the same chromosome • Linked during transmission from parent to offspring • Inherited like single genes • Recombination can break linkage ...
Presentation - American Society for Experimental NeuroTherapeutics
Presentation - American Society for Experimental NeuroTherapeutics

... “The homozygous form of the autosomal dominant disorder, familial hypercholesterolemia, is characterized by the presence in children of profound hypercholesterolemia, cutaneous planar xanthomas, and rapidly progressive coronary vascular disease that usually results in death before age 30 years. ….” ...
BDOL Interactive Chalkboard
BDOL Interactive Chalkboard

... Linkage maps • Because humans have only a few ___________ compared with the larger numbers of offspring in some other species, and because a human generation time is so long, mapping by linkage data is extremely inefficient. • Biotechnology now has provided scientists with new methods of __________ ...
Chapter 15 Chromosomal Basis of Inheritance
Chapter 15 Chromosomal Basis of Inheritance

... recessive to wild-type red eye color. • F2 - 3:1 red : white but all white eyes were MALE! ...
Meiosis and Mendel
Meiosis and Mendel

... twisted ladder (double helix) Despite all the genetic diversity on the planet, there are only 4 base pairs in DNA Adenine, Guanine, Cytosine and Thymine ...
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Title

... a. Alleles segregate during gamete formation b. Alleles segregate independently of alleles on the same chromosome c. Alleles segregate independently of alleles on different chromosome d. Gametes only have one of each allele ...
MCDB 1041 3/9/12 Activity 6: Central Dogma Continued PART I
MCDB 1041 3/9/12 Activity 6: Central Dogma Continued PART I

... an enzyme called phenylalanine hydroxylase. This enzyme breaks down the amino acid phenylalanine, which is important because phenylalanine is toxic when it accumulates in nerve cells, and can cause brain damage in children. Most proteins contain some of the amino acid phenylalanine, so people with P ...
Your Task
Your Task

... Libraries of RNAi construct allow similar screens for worms and flies. Mammalian screens are becoming possible as well ...
PowerPoint PDF Printout
PowerPoint PDF Printout

... In the US, sickle cell disease is most prevalent among African Americans. Caused by a mutation in the hemoglobin-Beta gene found on chromosome 11. Hemoglobin transports oxygen from the lungs to other parts of the body. Red blood cells with normal hemoglobin (hemoglobin-A) are smooth and round and ea ...
DNA shuffling by random fragmentation and reassembly: In
DNA shuffling by random fragmentation and reassembly: In

... method for multiple cycles of mutagenesis (8-10), so far no methods have been developed for general, homologous recombination of DNA in vitro. A technically simple approach to recombination is reported, and its application-to the design of linear sequences such as DNA, RNA, and proteins is explored ...
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... Know what restriction enzymes, “sticky ends” are, their function, and how they are useful in recombinant technology, where restriction enzymes come from Know the functions of promoter, operator, enhancer, regulator sites on DNA Know the different levels of control/their order in gene activity/expres ...
Bacteria - REMC 8 / Kent ISD Moodle VLE
Bacteria - REMC 8 / Kent ISD Moodle VLE

...  •During conjugation, DNA is exchanged between two bacterial cells (usually of the same species).  •In this process, the movement of DNA is one-way. There is a donor bacterium and a recipient.  •Exchange is facilitated by use of a conjugation pilus.  •The pilus pulls the two bacterial cells toge ...
Host cells for the production of biopharmaceuticals
Host cells for the production of biopharmaceuticals

...  Infection of cultured insect cells with an engineered baculovirus (a viral family that naturally infects insects) carrying the gene coding for a target protein  Most commonly used systems ...
Whose got Genes?
Whose got Genes?

... fruits that would result from crossbreeding two plants in his father’s garden Picture taken from biography.com Baker 2003/2004 ...
Unlocking Relationships with DNA
Unlocking Relationships with DNA

... DNA – (Deoxyribonucleic acid) the genetic code that makes each of us unique, the genetic code that has been passed down through generations Exact match – comparison between the DNA of two people that are exactly the same for all markers and regions compared Gene – a region of DNA that codes for a sp ...
McElwain, Mark: A Critical Review of Gene Prediction Software
McElwain, Mark: A Critical Review of Gene Prediction Software

... number of genomes sequenced numbering over one hundred, it is clear that quick, accurate annotation of predicted genes residing in these genomes is essential to learning more about biology and the evolutionary relationships between species. In the days of classical, forward genetics, the presence of ...
11-GeneTech
11-GeneTech

... plasmids with DNA fragments spanning the entire genome of another organism is called a __________________. A _____________ consists of short DNA molecules of a specific sequence that are used to identify which bacteria contain recombinant DNA for a specific gene. DNA sequencing phosphodiester endonu ...
Functional genomics
Functional genomics

... Inactivation of Gsk3 by AKT causes accumulation of b-catenin in Alveolar Macrophages Multi-step Regulation of Transcription by Pitx2 Presenilin action in Notch and Wnt signaling ...
Biology Fact Sheet
Biology Fact Sheet

... 1. Adhesion: water molecules' attraction for other molecules. For example, is the ability of water to climb small tubes by sticking to the walls or water sticking to blood vessels 2. Cohesion: water molecules cling to each other due to hydrogen bonding; the surface film (top layer of water) is held ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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