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Syllabus
Syllabus

... about the course to this site at any time and they will be answered by either a fellow student, a mentor, or me. Your questions may be more general and may relate to the course itself or they may be more specific and instead relate directly to content and/or material from class. In any case, Piazza ...
DNA sequence of the rat growth hormone gene: location of the 5
DNA sequence of the rat growth hormone gene: location of the 5

... All d i g e s t i o n s v/ere done with enzymes purchased fran e i t h e r Mew England Biolabs, rtethesda Pesearch Laboratories, o r Poehringer f'annheim. Digestions were usually done with a s u b s t a n t i a l excess of enzyme and approximately in accordance with t h e conditions provided by t h ...
Evidence for Natural Selection bollworm/insects
Evidence for Natural Selection bollworm/insects

... caterpillar pest of cotton in Arizona is a different species, known as pink bollworm (Pectinophora gossypiella), which has remained susceptible to the Bt toxin in biotech cotton. U.S. farmers planned to plant 13.2 million acres of cotton in 2007, according to the National Cotton Council of America. ...
IGA 8/e Chapter 4
IGA 8/e Chapter 4

Corneal dystrophies in Japan
Corneal dystrophies in Japan

... the stroma. Although the type of mutation is manifested by different histopathological findings, most patients with LCD, ACD, and GDLD showed amyloid deposits in several parts of the subepithelial layer and/or stromal layer. However, amyloid deposits were not observed in GCDGI, which in the classic ...
(b).
(b).

... The possible genotype combinations of the offspring are: Rr, Rr, rr, and rr We now know that the probability of the offspring having a red phenotype is 2/4 or 50% or a white phenotype 2/4 or 50%. ...
Shallow Gene Pool – No Diving! The Study of Cell Reproduction
Shallow Gene Pool – No Diving! The Study of Cell Reproduction

... 1. DNA – deoxyribonucleic acid, which is the genetic material of all organisms, made up of two twisted strands of sugar-phosphate molecules and nitrogen bases. 2. gene – section of DNA on a chromosome that contains instructions for making specific proteins. 3. mutation – any permanent change in a ge ...
Plant and animal microRNAs: similarities and differences
Plant and animal microRNAs: similarities and differences

... operating in a common ancestor, but originated independently from a more ancient system. This may not be so surprising considering that the last common ancestor of plants and animals was unicellular, and developmental comparisons have shown that the molecular mechanisms that gave rise to multicellul ...
Comparison of Statistical Models for Affymetrix GeneChip
Comparison of Statistical Models for Affymetrix GeneChip

... Model-based estimators are superior to simple averaging Full model superior to reduced this does not necessarily mean that the mismatch probes are a good idea - but if they are present we should use them we have demonstrated this using both analytic considerations and experimental data a carefully d ...
Chapter 11 and 12 from Campbell Biology 10th Edition By Keshara
Chapter 11 and 12 from Campbell Biology 10th Edition By Keshara

... phenotypic character (coat color) they follow the law of independent assortment  represents an F1 dithered cross  as result of epistatis the phenotypic ratio of G2 offspring is 9:3:4 and  other epistatis produce different rations but are all modified versions of 9:3:3:1 Polygenic inheritance  fo ...
I - Angelfire
I - Angelfire

... c. Inversions occur when the fragment reattaches to the original chromosome, but only after flipping around. d. Reciprocal translations occur when two portions of non homologous chromosomes trade places. ii. Organisms that have large deletion from all inherited copies of their sex chromosomes usuall ...
Presentation
Presentation

... Key features of DNA: • A double-stranded helix, uniform diameter • It is right-handed • It is antiparallel • Outer edges of nitrogenous bases are exposed in the major and minor grooves ...
Sonogenetics: A Breakthrough in Prenatal Diagnosis
Sonogenetics: A Breakthrough in Prenatal Diagnosis

... aberrations leading to improved genotype-phenotype correlation. However, aCGH cannot detect truly balanced chromosomal rearrangements or polypoidy, and may even generate data with unknown significance. Knowing its limitations and with proper counseling of the advantages and shortcomings, aCGH will b ...
(VHL) for families - Cancer Council Australia
(VHL) for families - Cancer Council Australia

... If a person is the first family member affected by VHL, the siblings and parents are at low risk of having VHL, but that person’s child has a 50% chance of inheriting VHL. ...
599 KB - CSIRO Publishing
599 KB - CSIRO Publishing

... mammals, or that ZFY was the wrong gene. Rather than the little ‘me too’ paper I had envisaged, Andrew and Jamie produced a cover story in Nature (Sinclair et al. 1988). Both students went on to make fundamental discoveries about human sex determination in Peter Goodfellow’s laboratory. Our conclusi ...
Telomere Shortening and Tumor Formation by Mouse Cells Lacking
Telomere Shortening and Tumor Formation by Mouse Cells Lacking

... Goal 3: Detection of telomerase activity in mTR-/- mice and mouse embryonic fibroblasts (MEF) MEF cultures and S100 extracts from brain, liver, thymus and spleen were tested for telomerase activity with the ...
chapter two neurons and glia
chapter two neurons and glia

... The Soma We begin our tour at the soma, the roughly spherical central part of the neuron. The cell body of the typical neuron is about 20 mm in diameter. The watery fluid inside the cell, called the cytosol, is a salty, potassiumrich solution that is separated from the outside by the neuronal membra ...
Caspary T, Cleary MA, Perlman EJ, Zhang P, Elledge SJ, and Tilghman SM. Genes Dev. 1999 Dec 1;13(23):3115-24. Oppositely imprinted genes p57Kip2 and Igf2 interact in a mouse model for Beckwith-Wiedemann syndrome.
Caspary T, Cleary MA, Perlman EJ, Zhang P, Elledge SJ, and Tilghman SM. Genes Dev. 1999 Dec 1;13(23):3115-24. Oppositely imprinted genes p57Kip2 and Igf2 interact in a mouse model for Beckwith-Wiedemann syndrome.

... in development, and thus a mechanistic connection between them could have gone undetected. Zhang et al. (1997) suggested that IGFII and p57 may act in opposing manners to control cell proliferation during development of human fetuses; that is, that a gain of function of IGF2 would act similarly to a ...
Life Cycle of a Glioma* From a Molecular Genomic
Life Cycle of a Glioma* From a Molecular Genomic

... Persistent stable restricted diffusion may represent areas of chronic ischemia and gelatinous material and not tumor ...
Three Allele Combinations Associated with
Three Allele Combinations Associated with

... Some studies searched for candidate gene combinations as MS risk factors; however, these studies have not yet extended beyond MS associations with alleles of each candidate gene coupled with HLA DRB1 alleles [13-19]. To examine the possibility that the combined effect of definite genes is a risk fac ...
SBI3U5.2DihybridCrossWorksheet
SBI3U5.2DihybridCrossWorksheet

... crosses. Predicting the outcome of two-factor crosses requires basically the same procedure as that for crosses involving one trait. During meiosis, nonhomologous chromosomes assort independently. This means that each of the chromosomes of any pair of homologous chromosomes has an equal probability ...
2010 HSC Exam Paper - Biology
2010 HSC Exam Paper - Biology

... An experiment was conducted to test the effect of dissolved carbon dioxide (CO2) on the pH of water. It was found that the pH of water decreased as CO2 was added. How do these findings relate to the acidity of blood as it circulates in the body? (A) Blood in the veins of muscles becomes less acidic ...
Notions of Biochemistry and Molecular Biology Manipulating DNA
Notions of Biochemistry and Molecular Biology Manipulating DNA

... Disorders related to dominant inheritance: brachydactyly (short fingers, 1905), congenital cataracts (1906), Huntington’s disease ...
Evolutionary interactions between sex chromosomes and autosomes
Evolutionary interactions between sex chromosomes and autosomes

... 1993), in which a parental gene transcribes, processes out introns, and then adds a poly-A tail to the 3 end of the retrogene and a pair of short duplicate sequences flanking the retrosequence. The ancestral relationship between parental and new copies can be easily discriminated by looking at the ...
Notch activity induces Nodal expression and mediates the
Notch activity induces Nodal expression and mediates the

... been mutated (Fig. 2C). These results suggest that perinodal expression of Nodal may be regulated by conserved promoter elements that depend directly on Notch activity. To investigate whether these elements are functional in vivo, we utilized a transgenic approach in mice. A 355-bp fragment of the m ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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