The C-terminus of S. pombe DDK subunit Dfp1 is
... Wild type homothallic (h90) cells undergoing meiosis produce asci with four regularly shaped spores and evenly segregated nuclei (Fig. 1B). However, h90 dfp1-r35 cells form asci with aberrant morphologies (Fig. 1B). Only about 24% of observed asci produce four spores. The majority (58%) produce two ...
... Wild type homothallic (h90) cells undergoing meiosis produce asci with four regularly shaped spores and evenly segregated nuclei (Fig. 1B). However, h90 dfp1-r35 cells form asci with aberrant morphologies (Fig. 1B). Only about 24% of observed asci produce four spores. The majority (58%) produce two ...
rational selection of pcr-based platforms for pharmacogenomic testing
... DNA microarrays and next-generation sequencing (NGS) are the two most important technologies for high-throughput genomic analysis. DNA microarray technology has been developed and consolidated as a routine tool in research laboratories and is now transitioning to the clinic. NGS technologies have em ...
... DNA microarrays and next-generation sequencing (NGS) are the two most important technologies for high-throughput genomic analysis. DNA microarray technology has been developed and consolidated as a routine tool in research laboratories and is now transitioning to the clinic. NGS technologies have em ...
THE STRUCTURE OF CHROMATIN
... structure of chromatin at a higher structural level than the direct effects on the gene promoters. We will discuss gene regulation at the promoter level before we finish talking about the nucleus two lectures from now. The effects of histone tail modification on gene activity that can be transmitted ...
... structure of chromatin at a higher structural level than the direct effects on the gene promoters. We will discuss gene regulation at the promoter level before we finish talking about the nucleus two lectures from now. The effects of histone tail modification on gene activity that can be transmitted ...
Within- and between-species DNA sequence variation and the
... under a model of free recombination among nucleotide sites and an equilibrium frequency distribution of mutations (Sawyer and Hartl, 1992; Hartl et al., 1994). The configuration of mutations for DNA sequences from a natural population, however, can be affected by population level phenomena, such as ...
... under a model of free recombination among nucleotide sites and an equilibrium frequency distribution of mutations (Sawyer and Hartl, 1992; Hartl et al., 1994). The configuration of mutations for DNA sequences from a natural population, however, can be affected by population level phenomena, such as ...
Chap 2 - Genetics
... or one million members in order to see an average of one mutation per generation for a gene with a mutation rate of 10-6. Most mutations are detrimental, and perhaps only 1 in 1,000 is beneficial. Thus, in this population of 1,000,000 we might have to wait for 1,000 years for a specific genetic locu ...
... or one million members in order to see an average of one mutation per generation for a gene with a mutation rate of 10-6. Most mutations are detrimental, and perhaps only 1 in 1,000 is beneficial. Thus, in this population of 1,000,000 we might have to wait for 1,000 years for a specific genetic locu ...
Molecular-3
... In some families, the defect is in the gene that encodes the receptor for luteinizing hormone (LCGR); these mutations constitutively activate the receptor's signaling action even in the absence of its hormone. The defect is not manifested in heterozygous females. Although the disease can be tran ...
... In some families, the defect is in the gene that encodes the receptor for luteinizing hormone (LCGR); these mutations constitutively activate the receptor's signaling action even in the absence of its hormone. The defect is not manifested in heterozygous females. Although the disease can be tran ...
Role of the Adenovirus Early Region 1 B Tumor Antigens in
... We have investigated the contribution of each of the two adenovirus type 5 (Ad5) major early region lb (Elb) proteins in cell transformation and in lytic infection. An Ad5 El plasmid, in which the reading frame for the 19kDa Elb protein was abolished by a stop codon close to the initiation codon, tr ...
... We have investigated the contribution of each of the two adenovirus type 5 (Ad5) major early region lb (Elb) proteins in cell transformation and in lytic infection. An Ad5 El plasmid, in which the reading frame for the 19kDa Elb protein was abolished by a stop codon close to the initiation codon, tr ...
PDF
... was predominantly in basal epidermal cells and not EVL cells from 24 hpf onwards in a number of ways. Firstly, through Nomarski optics, we could identify unlabelled EVL cells overlying the labelled cells (Figure 1B; Figure S2E, S2G). Secondly, we could see labelled cells outline the primordium (Figu ...
... was predominantly in basal epidermal cells and not EVL cells from 24 hpf onwards in a number of ways. Firstly, through Nomarski optics, we could identify unlabelled EVL cells overlying the labelled cells (Figure 1B; Figure S2E, S2G). Secondly, we could see labelled cells outline the primordium (Figu ...
Genetic Regulation of Meristem Maintenance and Organ
... determined; genetic transformation is relatively easy; many transposonmediated mutation lines are available to identify specific knockout mutants; and molecular tools such as microarray analysis are available. Recently, many genes that play essential roles in rice development have been isolated by p ...
... determined; genetic transformation is relatively easy; many transposonmediated mutation lines are available to identify specific knockout mutants; and molecular tools such as microarray analysis are available. Recently, many genes that play essential roles in rice development have been isolated by p ...
A Comparative Genomic Analysis of Two Distant Diptera, the Fruit
... comparative study of genome organization in the main African malaria vector, Anopheles gambiae, relative to the recently determined sequence of the Drosophila melanogaster genome. The ancestral lines of these two dipteran insects are thought to have separated ∼250 Myr, a long period that makes this ...
... comparative study of genome organization in the main African malaria vector, Anopheles gambiae, relative to the recently determined sequence of the Drosophila melanogaster genome. The ancestral lines of these two dipteran insects are thought to have separated ∼250 Myr, a long period that makes this ...
Bacteroides mobilizable and conjugative genetic elements
... rteA and rteB have been shown to be in the same operon as tetQ, production of these proteins is presumably due to translational coupling one ribosomes begin to translate the operon mRNA. RteA and RteB have no role in tetracycline regulation of operon expression. This was somewhat surprising because ...
... rteA and rteB have been shown to be in the same operon as tetQ, production of these proteins is presumably due to translational coupling one ribosomes begin to translate the operon mRNA. RteA and RteB have no role in tetracycline regulation of operon expression. This was somewhat surprising because ...
The past, present and future of plant breeding
... the light of day; first in the form of mutation breeding around 1960 and later using recombinant DNA technology or GM technology in 1983. Increasing knowledge of plant genetics and above all, improved methods of DNA analysis, led to an upgrade of existing techniques (such as cross-breeding) to arriv ...
... the light of day; first in the form of mutation breeding around 1960 and later using recombinant DNA technology or GM technology in 1983. Increasing knowledge of plant genetics and above all, improved methods of DNA analysis, led to an upgrade of existing techniques (such as cross-breeding) to arriv ...
Supplementary Figure 1. Generation of IDH2 mutant mice. (A
... Supplementary Figure 4. Reduced lipogenesis and stimulation of thermogenesis in adipose tissues of IDH2-deficient mice on a normal diet. (A) Quantifications of lipogenesis signal in sections of visceral WAT from wild-type and IDH2-/- mice. Quantifications of the levels of p53 and lipogenic protein ...
... Supplementary Figure 4. Reduced lipogenesis and stimulation of thermogenesis in adipose tissues of IDH2-deficient mice on a normal diet. (A) Quantifications of lipogenesis signal in sections of visceral WAT from wild-type and IDH2-/- mice. Quantifications of the levels of p53 and lipogenic protein ...
Variation in Drosophila melanogaster central metabolic genes
... variables in the form of latitudinal clines. Over the past four decades, many geographical clines in molecular polymorphism have been reported in many species (e.g. [1–7]), and these observations are often taken prima facie as evidence of natural selection. While geographical clines are an attractiv ...
... variables in the form of latitudinal clines. Over the past four decades, many geographical clines in molecular polymorphism have been reported in many species (e.g. [1–7]), and these observations are often taken prima facie as evidence of natural selection. While geographical clines are an attractiv ...
DNA and the Book of Mormon: A Phylogenetic Perspective
... the Human Genome Project is not really a completion of DNA work at all, but simply one step on the road toward a better understanding of ourselves as biological organisms, our shared genetic history as humans, and the genetic history we share with all living organisms. Work is under way in many fiel ...
... the Human Genome Project is not really a completion of DNA work at all, but simply one step on the road toward a better understanding of ourselves as biological organisms, our shared genetic history as humans, and the genetic history we share with all living organisms. Work is under way in many fiel ...
Whole genome analyses using PopGenome and VCF files
... the parameter format have to be set to format="VCF". In contrast to the readVCF function the data does not need to be compressed or tabixed and additionaly supports polyploid (e.g tetraploid) genotypes. However, each VCF file is completely loaded into the RAM and interpreted via efficient C Code wh ...
... the parameter format have to be set to format="VCF". In contrast to the readVCF function the data does not need to be compressed or tabixed and additionaly supports polyploid (e.g tetraploid) genotypes. However, each VCF file is completely loaded into the RAM and interpreted via efficient C Code wh ...
1 Defining the epigenetic mechanism of asymmetric cell division of
... borne epigenetic mechanism of asymmetric cell division, which might be required for cellular differentiation in higher organisms as well. The S. pombe mating-type switching process requires three loci, called “cassettes”: mating-type locus (mat1) contains either a P or an M allele, and that determi ...
... borne epigenetic mechanism of asymmetric cell division, which might be required for cellular differentiation in higher organisms as well. The S. pombe mating-type switching process requires three loci, called “cassettes”: mating-type locus (mat1) contains either a P or an M allele, and that determi ...
Association between IGF1R / i16 / TaqI and IGF1 / SnaBI
... frequency of IGF1/SnaBI allele A occurred similar to that of allele B (0.55 and 0.45, respectively). No effect was found of IGF1 genotype on milk production traits. In the case of IGF1R polymorphisms detected by TaqI digestion (frequency of allele A = 0.61 and 0.39 of allele B), no significant effec ...
... frequency of IGF1/SnaBI allele A occurred similar to that of allele B (0.55 and 0.45, respectively). No effect was found of IGF1 genotype on milk production traits. In the case of IGF1R polymorphisms detected by TaqI digestion (frequency of allele A = 0.61 and 0.39 of allele B), no significant effec ...
Transgenic Tobacco Plants with Bacillus thuringiensis 8
... which leads to osmotic imbalance and cell lysis (Hofmann et ai., 1988; Slatin et ai., 1990; English et al., 1991; English and Slatin, 1992). Among insecticidal protein genes, lepidopteran specific genes, cryJA class, are mostly well characterized (Adang et al., 1985; Thorne et al., 1986; Schnepf et ...
... which leads to osmotic imbalance and cell lysis (Hofmann et ai., 1988; Slatin et ai., 1990; English et al., 1991; English and Slatin, 1992). Among insecticidal protein genes, lepidopteran specific genes, cryJA class, are mostly well characterized (Adang et al., 1985; Thorne et al., 1986; Schnepf et ...
Gene conversion and purifying selection shape nucleotide variation
... When a sample from a male showed two nucleotide peaks at one or more sites (“heterozygous” sites) in the L or M opsin gene, the individual should have two or more loci of the gene with different sequences. In this case, we conservatively inferred two loci for this gene. When a female showed heterozy ...
... When a sample from a male showed two nucleotide peaks at one or more sites (“heterozygous” sites) in the L or M opsin gene, the individual should have two or more loci of the gene with different sequences. In this case, we conservatively inferred two loci for this gene. When a female showed heterozy ...
Application Note: Targeted sequencing and chromosomal haplotype
... amplification of any genomic locus of interest over 50 kb using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works ...
... amplification of any genomic locus of interest over 50 kb using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse