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SynCAM2a ΔPDZ Δ4.1B ΔPDZ - University of Oregon (SPUR)
... Subclone 4 SynCAM2a constructs (full-length, and 3 deletions: 4.1B, PDZ, and 2X [both protein binding domains] deletions into a vector with a fluorescent tag. Microinject zebrafish embryos with the 4 constructs. Stain known pre and postsynaptic proteins through immunohistochemistry (IHC) to de ...
... Subclone 4 SynCAM2a constructs (full-length, and 3 deletions: 4.1B, PDZ, and 2X [both protein binding domains] deletions into a vector with a fluorescent tag. Microinject zebrafish embryos with the 4 constructs. Stain known pre and postsynaptic proteins through immunohistochemistry (IHC) to de ...
Hypertension-Associated Transcription Factor (HATF-1)
... expressed in SHR brains as early as 3 days post-partum. Northern blot analysis over a developmental timecourse from 3 to 10 days post-partum indicates a two to four fold increase in mRNA levels in SHR brains compared to the normotensive controls. This transcript, which we have named Hypertension-ass ...
... expressed in SHR brains as early as 3 days post-partum. Northern blot analysis over a developmental timecourse from 3 to 10 days post-partum indicates a two to four fold increase in mRNA levels in SHR brains compared to the normotensive controls. This transcript, which we have named Hypertension-ass ...
Powerpoint file - revised
... Therefore, transcriptional initiation is usually the major control point. Most prokaryotic genes are regulated in units called operons (Jacob and Monod, 1960) Operon: a coordinated unit of gene expression consisting of one or more related genes and the operator and promoter sequences that regulate t ...
... Therefore, transcriptional initiation is usually the major control point. Most prokaryotic genes are regulated in units called operons (Jacob and Monod, 1960) Operon: a coordinated unit of gene expression consisting of one or more related genes and the operator and promoter sequences that regulate t ...
Slides - University of Minnesota
... • Shown correlation with structure, sequence and phylogeny • Explored structure-function relation via FDUG (functional domain universe graph) • Indicated examples of divergent and convergent evolution ...
... • Shown correlation with structure, sequence and phylogeny • Explored structure-function relation via FDUG (functional domain universe graph) • Indicated examples of divergent and convergent evolution ...
Full-Text PDF
... localization signal. It contains two conserved helical regions, one located in the central region (residues 75–95) and one in the C-terminus (residues 143–193), separated from each other and from the N-terminus by regions predicted to be intrinsically disordered. Neither of the conserved helical reg ...
... localization signal. It contains two conserved helical regions, one located in the central region (residues 75–95) and one in the C-terminus (residues 143–193), separated from each other and from the N-terminus by regions predicted to be intrinsically disordered. Neither of the conserved helical reg ...
Gene Regulation -
... Definitions: P strands for promoter; it is the site where RNA polymerase attaches in order to transcribe mRNA. Although all promoters have the same function and share similar sequences that are recognized by RNA polymerase, they differ enough so that some are very strong (leading to high levels of t ...
... Definitions: P strands for promoter; it is the site where RNA polymerase attaches in order to transcribe mRNA. Although all promoters have the same function and share similar sequences that are recognized by RNA polymerase, they differ enough so that some are very strong (leading to high levels of t ...
model 1 - Instructure
... - The β-galactosidase protein in bacteria is actually coded for by a gene called LacZ that is part of a unique construct called an "operon". We'll discuss the structure of the "Lac operon" in lab. However, the fact that this gene is part of an operon has no inherent effect on how it is regulated. Th ...
... - The β-galactosidase protein in bacteria is actually coded for by a gene called LacZ that is part of a unique construct called an "operon". We'll discuss the structure of the "Lac operon" in lab. However, the fact that this gene is part of an operon has no inherent effect on how it is regulated. Th ...
Topic 6
... In any study of development, physiology or disease the objective is to use as many appropriate techniques as possible to gain understanding. However, for the majority of such studies specific genes are the natural center point of investigation. This is because analysis and manipulation of nucleic ac ...
... In any study of development, physiology or disease the objective is to use as many appropriate techniques as possible to gain understanding. However, for the majority of such studies specific genes are the natural center point of investigation. This is because analysis and manipulation of nucleic ac ...
Protein Complexes – Challenges and Opportunities for
... It is estimated that up to 250.000 protein products are encoded by our genome, and even if only a minor portion is expressed at relevant levels in any type of cell, the number of potential interactions and assemblies is beyond imagination. Thus, their systematic identification is a major technical c ...
... It is estimated that up to 250.000 protein products are encoded by our genome, and even if only a minor portion is expressed at relevant levels in any type of cell, the number of potential interactions and assemblies is beyond imagination. Thus, their systematic identification is a major technical c ...
The Automation of Protein Expression
... and arrays, and raising antibodies, scientists increasingly require samples of protein for research. However, protein expression is considered to be a complex, lengthy procedure, and some proteins will not express at all. This article describes the strategy adopted by NextGen Sciences to develop and ...
... and arrays, and raising antibodies, scientists increasingly require samples of protein for research. However, protein expression is considered to be a complex, lengthy procedure, and some proteins will not express at all. This article describes the strategy adopted by NextGen Sciences to develop and ...
Abstract
... Abstract Protein kinases mediate most intracellular signal transduction via the reversible phosphorylation on serine, threonine, or tyrosine residue of specific protein/peptide substrates. Such phosphorylation is employed by all eukaryotes in regulation of enzyme activity, protein-protein interactio ...
... Abstract Protein kinases mediate most intracellular signal transduction via the reversible phosphorylation on serine, threonine, or tyrosine residue of specific protein/peptide substrates. Such phosphorylation is employed by all eukaryotes in regulation of enzyme activity, protein-protein interactio ...
Molecular Genetics
... – added and a poly-A tail added to the 3’ end – (Note: cap and tail are protection from degradation and recognition by ribosome) – Spliced out introns (non-coding segments; the coding segments are called exons) ...
... – added and a poly-A tail added to the 3’ end – (Note: cap and tail are protection from degradation and recognition by ribosome) – Spliced out introns (non-coding segments; the coding segments are called exons) ...
DNA/Protein structure-function analysis and prediction - IBIVU
... 3D domain swapping definitions. A: Closed monomers are comprised of tertiary or secondary structural domains (represented by a circle and square) linked by polypeptide linkers (hinge loops). The interface between domains in the closed monomer is referred to as the C- (closed) interface. Closed monom ...
... 3D domain swapping definitions. A: Closed monomers are comprised of tertiary or secondary structural domains (represented by a circle and square) linked by polypeptide linkers (hinge loops). The interface between domains in the closed monomer is referred to as the C- (closed) interface. Closed monom ...
Student Activity PDF - TI Education
... the activity of a cell? DNA is transcribed into RNA, which can be translated into the proteins that drive the activity in a cell. This is the Central Dogma of biology: DNA RNA Protein In this lesson, you will explore transcription and translation. ...
... the activity of a cell? DNA is transcribed into RNA, which can be translated into the proteins that drive the activity in a cell. This is the Central Dogma of biology: DNA RNA Protein In this lesson, you will explore transcription and translation. ...
8.4 Transcription
... The transcription process is similar to replication. • Transcription and replication both involve complex enzymes and complementary base pairing. • The two processes have different end results. – Replication copies all the DNA in cell; transcription copies a specific gene on a strand of DNA. ...
... The transcription process is similar to replication. • Transcription and replication both involve complex enzymes and complementary base pairing. • The two processes have different end results. – Replication copies all the DNA in cell; transcription copies a specific gene on a strand of DNA. ...
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... positions, serving as the grooves of a screw to direct the DNA wrapping around the histone core in a left-handed manner. ...
... positions, serving as the grooves of a screw to direct the DNA wrapping around the histone core in a left-handed manner. ...
2nd lesson Medical students Medical Biology
... known as the Central Dogma of molecular biology and is an underlying theme in all studies of gene expression. Transcription and translation These two processes are the critical steps involved in producing functional proteins in the cell. Transcription involves synthesis of an RNA from the DNA templa ...
... known as the Central Dogma of molecular biology and is an underlying theme in all studies of gene expression. Transcription and translation These two processes are the critical steps involved in producing functional proteins in the cell. Transcription involves synthesis of an RNA from the DNA templa ...
2nd lesson Medical students Medical Biology
... known as the Central Dogma of molecular biology and is an underlying theme in all studies of gene expression. Transcription and translation These two processes are the critical steps involved in producing functional proteins in the cell. Transcription involves synthesis of an RNA from the DNA templa ...
... known as the Central Dogma of molecular biology and is an underlying theme in all studies of gene expression. Transcription and translation These two processes are the critical steps involved in producing functional proteins in the cell. Transcription involves synthesis of an RNA from the DNA templa ...
A New Twist on Transcriptional Bursting
... an effective stall force that eventually brings transcription to a halt. The key insight is that this unsynchronized push and pull between transcription and torsion might be responsible for bursting. The authors first test this idea in vitro by observing transcription from torsionally constrained 12 ...
... an effective stall force that eventually brings transcription to a halt. The key insight is that this unsynchronized push and pull between transcription and torsion might be responsible for bursting. The authors first test this idea in vitro by observing transcription from torsionally constrained 12 ...
Gene Regulation
... DNA regulatory sequences are tissue-specific. • Muscle-specific genes all have a particular sequence in their DNA to which a muscle-specific transcription factor binds and activates expression of these genes. • Nerve-specific genes have a DIFFERENT sequence in the DNA and different transcription fa ...
... DNA regulatory sequences are tissue-specific. • Muscle-specific genes all have a particular sequence in their DNA to which a muscle-specific transcription factor binds and activates expression of these genes. • Nerve-specific genes have a DIFFERENT sequence in the DNA and different transcription fa ...
Expression patterns of PRDM10 during mouse embryonic
... Our present study suggests that PRDM10 expression stimulates the sophisticated morphogenesis of tissue differentiation, including somites and craniofacial tissue formation. Therefore, we postulate BMB reports ...
... Our present study suggests that PRDM10 expression stimulates the sophisticated morphogenesis of tissue differentiation, including somites and craniofacial tissue formation. Therefore, we postulate BMB reports ...
Errata - Blood Journal
... Figure 7. EGFL7 interacts with Notch receptors and regulates Notch target gene expression in vivo. (A) Alignment of the DSL domain of Jagged, Serrate, Delta, and Lag-2 with the putative DSL domain in EGFL7. Red letters represent the consensus sequence. (B) Yeast-2-hybrid assay (left panel): EGFL7 in ...
... Figure 7. EGFL7 interacts with Notch receptors and regulates Notch target gene expression in vivo. (A) Alignment of the DSL domain of Jagged, Serrate, Delta, and Lag-2 with the putative DSL domain in EGFL7. Red letters represent the consensus sequence. (B) Yeast-2-hybrid assay (left panel): EGFL7 in ...
Chapter 15 Gene Regulation Prokaryotic Regulation
... – All cells have the complete set of genetic information – Cells perform different ‘tasks’ and have different functions – phenotypic specialization at the cellular and tissue levels ...
... – All cells have the complete set of genetic information – Cells perform different ‘tasks’ and have different functions – phenotypic specialization at the cellular and tissue levels ...
Histone acetylation and deacetylation
![](https://commons.wikimedia.org/wiki/Special:FilePath/Nucleosome_1KX5_colour_coded.png?width=300)
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.