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Aucsia Gene Silencing Causes Parthenocarpic Fruit Development in
Aucsia Gene Silencing Causes Parthenocarpic Fruit Development in

... One of these clones, a 129-bp-long cDNA fragment amplified with the BstCC-MseC primer combination (see cDNA sequence in Supplemental Fig. S1A), was identical to a portion of an EST (BG126668) and of a full-length cDNA (AK224828 and BW690880) of tomato. This gene, which we have named SlAucsia-1, was ...
Evolution of the Actin Gene Family in Testate Lobose Amoebae
Evolution of the Actin Gene Family in Testate Lobose Amoebae

... have performed all PCRs using three times the recommended extension time and 2- to 4-fold more concentrated primers according to general guidelines for chimera reduction (Judo et al. 1998). Determining a Data Set of ‘‘Real’’ Sequences We chose a conservative approach to distinguish ‘‘real’’ and ‘‘ch ...
Molecular Genetics and Genomics
Molecular Genetics and Genomics

... site, is located on the 3.7-kb PstI fragment. R. leguminosarum bv trifolii RS800 harbors three plasmids with molecular weights of about 190, 280, and 470 MDa (Rodriguez-QuinÄones et al. 1989). In order to locate the exoB gene in the genome of RS800, a blotted Eckhardt gel (Rosenberg et al. 1982) was ...
Evolution of meiosis genes in sexual vs. asexual Potamopyrgus
Evolution of meiosis genes in sexual vs. asexual Potamopyrgus

... transmitted genomes. These comparisons will provide powerful tests of hypotheses for pseudogenization and decay of asexual meiosis genes. The expectations of meiosis gene decay in asexuals are complicated by the fact that some asexual lineages use alternate forms of meiosis, likely mediated by modif ...
Non contiguous-finished genome sequence and description of
Non contiguous-finished genome sequence and description of

Slide 1
Slide 1

... • Many coding genes are well annotated with their biological functions. Non-coding regions typically lack such annotation. GREAT assigns biological meaning to a set of non-coding genomic regions by analyzing the annotations of the nearby genes. Thus, it is particularly useful in studying functions o ...
Genetic Causes of Phenotypic Adaptation to the Second
Genetic Causes of Phenotypic Adaptation to the Second

... particularly well-adapted strains. In this study, we investigated the behavior of S. cerevisiae wine strains during the production of sparkling wines such as Champagne and Cava. This particular winemaking process consists of two consecutive fermentations. A primary fermentation is conducted to obtai ...
Will my baby have PKU? pp PP Pp Pp Pp Pp pp Pp Pp Pp pp pp Pp
Will my baby have PKU? pp PP Pp Pp Pp Pp pp Pp Pp Pp pp pp Pp

... If you have PKU, the chance that any of your children will have PKU depends on whether your partner is a carrier. If your partner is not a carrier (see Figure 2) he/she has only normal PKU genes to pass on. All of your children inherit a mutation from you and will be carriers. If your partner is a c ...
Datasheet - Santa Cruz Biotechnology
Datasheet - Santa Cruz Biotechnology

... BACKGROUND ...
robbie0306
robbie0306

... Method due to George and Robbie Take two networks and scramble the name mapping between them. ...
Chapter 2: Mendelian Inheritance
Chapter 2: Mendelian Inheritance

... 4. Mendel conducted crosses between variants of a single trait. a. called a monohybrid or single-factor cross Mendel Followed the Outcome of a Single Character for Two Generations 1. Mendel conducted experiments to determine the mathematical relationship between hereditary traits. a. this process is ...
Chapter 2: Mendelian Inheritance
Chapter 2: Mendelian Inheritance

... 4. Mendel conducted crosses between variants of a single trait. a. called a monohybrid or single-factor cross Mendel Followed the Outcome of a Single Character for Two Generations 1. Mendel conducted experiments to determine the mathematical relationship between hereditary traits. a. this process is ...
univERsity oF copEnhAGEn
univERsity oF copEnhAGEn

... Figure 1. Alignment of the FCN1 nucleotide sequences. Hum REF is the human reference sequence containing the major alleles. Gene variation among a species is given by R (A or G), Y (C or T), K(G or T), M (A or C), S (G or C) or W (A or T). doi:10.1371/journal.pone.0028187.g001 ...
Chapter 20
Chapter 20

... manipulation of genes for practical purposes • DNA technology has revolutionized biotechnology, the manipulation of organisms or their genetic components to make useful products • An example of DNA technology is the microarray, a measurement of gene expression of thousands of different genes Copyrig ...
Activation of proto-oncogenes by disruption of
Activation of proto-oncogenes by disruption of

... The boundaries of chromosome neighborhoods may be disrupted in other cancers. A recent study noted that mutations in CTCF binding sites occur frequently in cancers (23), but it is unclear if mutations in boundaries are common as only a subset of CTCF sites form insulated neighborhoods (8, 10, 24). C ...
Class III malocclusion. Role of nature and nurture
Class III malocclusion. Role of nature and nurture

... improved orofacial function. However some ...
The genetics of deafness - Archives of Disease in Childhood
The genetics of deafness - Archives of Disease in Childhood

... have been roughly localised, they will in due course be cloned and their protein products become available for ...
Chapter 20 powerpoint - Bremen High School District 228
Chapter 20 powerpoint - Bremen High School District 228

... manipulation of genes for practical purposes • DNA technology has revolutionized biotechnology, the manipulation of organisms or their genetic components to make useful products • An example of DNA technology is the microarray, a measurement of gene expression of thousands of different genes Copyrig ...
Article The Landscape of Realized Homologous
Article The Landscape of Realized Homologous

... affects multiple bases. Part of the measured spatial autocorrelation that we observe therefore reflects the stochastic sampling effects due to the influence of individual events on our statistic, rather than spatial autocorrelation in the underlying distribution of recombination rates. This means th ...
Chapter 20
Chapter 20

... manipulation of genes for practical purposes • DNA technology has revolutionized biotechnology, the manipulation of organisms or their genetic components to make useful products • An example of DNA technology is the microarray, a measurement of gene expression of thousands of different genes Copyrig ...
Sample final exam questions – BI/FS 430(H)/530
Sample final exam questions – BI/FS 430(H)/530

Genes in conflict: the biology of selfish genetic elements
Genes in conflict: the biology of selfish genetic elements

... the process is thought to have gone to completion: there are no mitochondria, but there are other organelles called hydrogenosomes that appear to be derived from mitochondria but do not contain any DNA at all (Embley et al. 2003). In all species, the vast majority of proteins in mitochondria are enc ...
Click here to presentation
Click here to presentation

... • XPO1 is the nuclear export factor for p65 & drives its recycling back out of the nucleus • p65 siRNA directly silences p65 • these controls enable understanding of the array quality in screening & XPO1 /p65 siRNA will give different phenotype to control – XPO1 will accumulate p65 in the nucleus – ...
Biosynthesis of flavonoids and effects of stress
Biosynthesis of flavonoids and effects of stress

... three-dimensional structures of these enzymes by Joe Noel’s group within the past few years [37,38•]. Most recently, this group has combined site-specific mutagenesis with analysis of X-ray crystal structures to correlate the size of the activesite cavity with the length of the polyketide chain prod ...
Expression of an Entire Bacterial Operon in Plants
Expression of an Entire Bacterial Operon in Plants

... The biosynthesis of a secondary metabolite involves numerous enzymes, the genes of which constitute a metabolic pathway. Attempts to introduce multiple genes into plants by various techniques (e.g., gene stacking) have been somewhat successful (Halpin, 2005). However, lack of co-regulation remains ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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