Arabidopsis onset of leaf death mutants identify a regulatory
... phenotypical, physiological and molecular markers. old1 is represented by three recessive alleles and displayed earlier senescence both in air and upon ethylene exposure. The etiolated old1 seedlings exhibited a hypersensitive triple response. old2 is a dominant trait and the mutant plants were indi ...
... phenotypical, physiological and molecular markers. old1 is represented by three recessive alleles and displayed earlier senescence both in air and upon ethylene exposure. The etiolated old1 seedlings exhibited a hypersensitive triple response. old2 is a dominant trait and the mutant plants were indi ...
1 RUNNING TITLE Diversity and selection of
... As expected, only wild type alleles for the fruit shape genes were found in the wild species (Table I). Moreover, none of the Latin American regional and cerasiforme accessions carried the mutation in SUN, suggesting that the gene duplication resulting in elongated fruit shape did not occur in South ...
... As expected, only wild type alleles for the fruit shape genes were found in the wild species (Table I). Moreover, none of the Latin American regional and cerasiforme accessions carried the mutation in SUN, suggesting that the gene duplication resulting in elongated fruit shape did not occur in South ...
informe tecnológico de patentes
... Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant allele. We present a small interfering RNA (siRNA) that selectively redu ...
... Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant allele. We present a small interfering RNA (siRNA) that selectively redu ...
"RNA Interference in Caenorhabditis elegans".
... et al., 2000). In the microinjection and soaking protocols, in vitro preparations of dsRNA are delivered mechanically using a needle to inject dsRNA directly into the body or passively by soaking worms in dsRNA solution. In the feeding protocols, dsRNA is transcribed in E. coli and ingested by anima ...
... et al., 2000). In the microinjection and soaking protocols, in vitro preparations of dsRNA are delivered mechanically using a needle to inject dsRNA directly into the body or passively by soaking worms in dsRNA solution. In the feeding protocols, dsRNA is transcribed in E. coli and ingested by anima ...
The Genetics of CFTR: Genotype – Phenotype Relationship
... behaviour that would point to the mutational class VI. It is in general quite difficult to classify a mutation without specific experimental studies aimed to its functional characterization. Due to the complexity of such studies, they have been performed only for a very limited number of the over 18 ...
... behaviour that would point to the mutational class VI. It is in general quite difficult to classify a mutation without specific experimental studies aimed to its functional characterization. Due to the complexity of such studies, they have been performed only for a very limited number of the over 18 ...
Comparative gene mapping in Arabidopsis lyrata chromosomes 6
... differ considerably. Their map distances may nevertheless be similar if recombination occurs largely within coding sequences, and if gene content is similar in homologous intervals in both species. Restriction of crossovers to the genes has previously been inferred from the similarity of genetic map ...
... differ considerably. Their map distances may nevertheless be similar if recombination occurs largely within coding sequences, and if gene content is similar in homologous intervals in both species. Restriction of crossovers to the genes has previously been inferred from the similarity of genetic map ...
Genetic and biochemical analysis of the adenylyl cyclase of
... as measured in vitro, but only modestly elevated cAMP levels. Such strains conjugate and sporulate less frequently than wild-type cells upon nutrient limitation. Strains which carry the wild-type cyrl gene but that also express high levels of the amino terminal domain of adenylyl cyclase behave much ...
... as measured in vitro, but only modestly elevated cAMP levels. Such strains conjugate and sporulate less frequently than wild-type cells upon nutrient limitation. Strains which carry the wild-type cyrl gene but that also express high levels of the amino terminal domain of adenylyl cyclase behave much ...
Human pigmentation variation: Evolution, genetic basis, and
... shaped the distribution of pigmentation according to a latitudinal gradient. Research during the last 5 years has substantially increased our understanding of the genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype as ...
... shaped the distribution of pigmentation according to a latitudinal gradient. Research during the last 5 years has substantially increased our understanding of the genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype as ...
Human pigmentation variation: Evolution, genetic basis, and
... shaped the distribution of pigmentation according to a latitudinal gradient. Research during the last 5 years has substantially increased our understanding of the genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype as ...
... shaped the distribution of pigmentation according to a latitudinal gradient. Research during the last 5 years has substantially increased our understanding of the genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype as ...
Resveratrol Opportunism: What Is the Science Behind the Claims?
... and sulfate forms of resveratrol also stay in the blood for some time. The question that recently has been raised is whether higher blood levels of the glucuronide and sulfate forms of resveratrol themselves have benefit, perhaps serving as a pool of “time release” sources of resveratrol between tim ...
... and sulfate forms of resveratrol also stay in the blood for some time. The question that recently has been raised is whether higher blood levels of the glucuronide and sulfate forms of resveratrol themselves have benefit, perhaps serving as a pool of “time release” sources of resveratrol between tim ...
Genetic and molecular regulation of fruit and plant domestication
... non-pungent peppers, generally of the bell-types, are economically the most important peppers and are part of the human diet throughout the world (Bosland and Votava, 2000). Bell peppers were first described approximately 500 years ago and the earliest variety used for cultivation was described in 1 ...
... non-pungent peppers, generally of the bell-types, are economically the most important peppers and are part of the human diet throughout the world (Bosland and Votava, 2000). Bell peppers were first described approximately 500 years ago and the earliest variety used for cultivation was described in 1 ...
Pharmacogenetics of warfarin: current status and future
... vitamin K, illness, age, gender, concurrent medication and body surface area, and by genetic variation.1–8 To be able to improve the benefit–harm profile associated with warfarin therapy, all these factors need to be taken into account. There is increasing interest in whether pharmacogenetics can ac ...
... vitamin K, illness, age, gender, concurrent medication and body surface area, and by genetic variation.1–8 To be able to improve the benefit–harm profile associated with warfarin therapy, all these factors need to be taken into account. There is increasing interest in whether pharmacogenetics can ac ...
The Homothorax homeoprotein activates the nuclear localization of
... HOX genes. In vitro studies showed that Exd alone can bind to specific DNA sequences (Lu and Kamps 1996), suggesting that it can act as a transcription factor by itself in vivo. We report the cloning and characterization of the homothorax (hth) gene in Drosophila. hth was found to act as a homeotic ...
... HOX genes. In vitro studies showed that Exd alone can bind to specific DNA sequences (Lu and Kamps 1996), suggesting that it can act as a transcription factor by itself in vivo. We report the cloning and characterization of the homothorax (hth) gene in Drosophila. hth was found to act as a homeotic ...
Publication - The University of Texas Health Science Center at
... HLA associations The major histocompatibility complex (MHC) or HLA region is the most polymorphic region of the genome. Polymorphisms in HLA have been linked to a number of autoimmune diseases including rheumatoid arthritis, ankylosing spondylitis, systemic lupus erythematosus, and many others [1,21 ...
... HLA associations The major histocompatibility complex (MHC) or HLA region is the most polymorphic region of the genome. Polymorphisms in HLA have been linked to a number of autoimmune diseases including rheumatoid arthritis, ankylosing spondylitis, systemic lupus erythematosus, and many others [1,21 ...
Design considerations for highly specific and efficient
... In order to understand the parameters affecting CRISPR-Cas9 gene editing efficiency, we systematically transfected synthetic CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA) reagents targeting components of the proteasome into a reporter cell line in which knockout of proteasome functio ...
... In order to understand the parameters affecting CRISPR-Cas9 gene editing efficiency, we systematically transfected synthetic CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA) reagents targeting components of the proteasome into a reporter cell line in which knockout of proteasome functio ...
The Etiology of ADHD: Behavioral and Molecular Genetic
... etiology of extreme scores on a continuous trait. Because a detailed description of this method is available elsewhere (e.g., DeFries & Fulker, 1985, 1988; Stevenson, 1992; Willcutt et al., 2003), I will summarize briefly the basic logic of the analysis and describe in more detail the results of stu ...
... etiology of extreme scores on a continuous trait. Because a detailed description of this method is available elsewhere (e.g., DeFries & Fulker, 1985, 1988; Stevenson, 1992; Willcutt et al., 2003), I will summarize briefly the basic logic of the analysis and describe in more detail the results of stu ...
C3H/HeJ
... between APN, an in-house strain with low CYP1A2 expression, and C3H/HeJ, a laboratory strain expressing normal CYP1A2 levels, determined that this phenotype is mediated by three quantitative trait loci (QTL) localized to chromosomes 1, 4 and 9, as previously reported. The QTL on chromosome 9 co-loca ...
... between APN, an in-house strain with low CYP1A2 expression, and C3H/HeJ, a laboratory strain expressing normal CYP1A2 levels, determined that this phenotype is mediated by three quantitative trait loci (QTL) localized to chromosomes 1, 4 and 9, as previously reported. The QTL on chromosome 9 co-loca ...
endoglucanase in cellulose biosynthesis is not very clear
... incubated with a radioactively labeled substrate, the 83-kD polypeptide bound to the substrate suggesting that it was the catalytic subunit (4). Amino acid sequences obtained from the 83-kD polypeptide and 93-kD polypeptide were used to clone the genes for cellulose synthase and other proteins (5). ...
... incubated with a radioactively labeled substrate, the 83-kD polypeptide bound to the substrate suggesting that it was the catalytic subunit (4). Amino acid sequences obtained from the 83-kD polypeptide and 93-kD polypeptide were used to clone the genes for cellulose synthase and other proteins (5). ...
Mutations at the Darkener of apricot Locus Modulate Transcript
... of wing-structure was compared among the four classes of wing tissue, Doa mutant heterozygotes intermediate segregants in the F1 generation. Since the Bx locus is X amounts, and Doa heteroallelic flies the most. Other linked, F, males were hemizygous for Bx'~,and females were Bx alleles similarly te ...
... of wing-structure was compared among the four classes of wing tissue, Doa mutant heterozygotes intermediate segregants in the F1 generation. Since the Bx locus is X amounts, and Doa heteroallelic flies the most. Other linked, F, males were hemizygous for Bx'~,and females were Bx alleles similarly te ...
here - Glaucoma Genetics Lab
... Purpose: To both characterize the clinical features of large primary open angle glaucoma (POAG) pedigree from a village in southern India and to investigate the genetic basis of their disease. Materials and methods: Eighty-four members of a large pedigree received complete eye examinations including ...
... Purpose: To both characterize the clinical features of large primary open angle glaucoma (POAG) pedigree from a village in southern India and to investigate the genetic basis of their disease. Materials and methods: Eighty-four members of a large pedigree received complete eye examinations including ...
Commentary: Wilhelm Johannsen and the problem of heredity at the
... Johannsen was also preparing his classical bean selection experiment. Inspired by the ongoing debate between Hugo de Vries and the British biometricians, Frank Raphael Weldon and Karl Pearson, he wanted to test Galton’s law of ancestral heredity. With the assumption that heredity varied continuously ...
... Johannsen was also preparing his classical bean selection experiment. Inspired by the ongoing debate between Hugo de Vries and the British biometricians, Frank Raphael Weldon and Karl Pearson, he wanted to test Galton’s law of ancestral heredity. With the assumption that heredity varied continuously ...
Figure legends Figure 1. Biosynthesis and catabolism of NAD+ in
... substantial changes were observed for the different tested conditions. Results are the means of three biological replicates with standard deviation. Supplemental Data S1. Graphical representations of the microarray data for the inducible foliar system. A, Venn diagram showing overlap between gene li ...
... substantial changes were observed for the different tested conditions. Results are the means of three biological replicates with standard deviation. Supplemental Data S1. Graphical representations of the microarray data for the inducible foliar system. A, Venn diagram showing overlap between gene li ...
File - Catecholaminergic Polymorphic Ventricular
... microarray. Hypothesis: There is be a noticeable expression level difference between samples from the two chambers. The expression level of RYR2 will change after exercise in RYR2 mutants but not in wild type mice. Aim 3: Determine if RYR2's binding parters are affecting RYR2's function differently ...
... microarray. Hypothesis: There is be a noticeable expression level difference between samples from the two chambers. The expression level of RYR2 will change after exercise in RYR2 mutants but not in wild type mice. Aim 3: Determine if RYR2's binding parters are affecting RYR2's function differently ...
Na concentration (root and shoot) (15%)
... Polygenic inheritance, also known as quantitative or multifactorial inheritance refers to inheritance of a phenotypic characteristic (trait) that is attributable to two or more genes and their interaction with the environment Unlike monogenic traits, polygenic traits do not follow patterns of Me ...
... Polygenic inheritance, also known as quantitative or multifactorial inheritance refers to inheritance of a phenotypic characteristic (trait) that is attributable to two or more genes and their interaction with the environment Unlike monogenic traits, polygenic traits do not follow patterns of Me ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.