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A Twist-like bHLH gene is a downstream factor of an
A Twist-like bHLH gene is a downstream factor of an

... 2002). In the present study, we use the term ‘mesenchyme’ for all of these cells, except where indicated otherwise. In addition to these three lineages of mesenchymal cells, ascidian larvae develop trunk ventral cells (TVCs), which are often regarded as a kind of mesenchymal cell because they give r ...
ANNEX III GUIDANCE ON COMMONLY USED VIRAL VECTORS
ANNEX III GUIDANCE ON COMMONLY USED VIRAL VECTORS

... recombination between vector and packaging sequences. These two component packaging lines should be used where possible. 20. It is good practice to demonstrate the absence of RCRs in vector stocks and mandatory where the level of containment proposed assumes that RCRs are not present. For most purpo ...
detection and pathogenetic role of mmr missense mutations
detection and pathogenetic role of mmr missense mutations

... is an autosomal dominant hereditary cancer syndrome, which accounts for 5% of all colorectal cancers. HNPCC is associated with an increased (90% for men, 70% for women) lifetime risk of endometrial, ovarian and other extra colonic cancers. HNPCC syndrome is defined in clinical terms by the revised B ...
Genetics Questions Extra - Science-with
Genetics Questions Extra - Science-with

... of black body. A grey-bodied male fly was allowed to breed with many black-bodied females. Some of the offspring had grey bodies and some had black bodies. a) What were the genotypes of the parents and the offspring? b) Two grey-bodied flies were mated, and all the offspring had grey bodies. Can you ...
Polyploidy Enhances F Pollen Sterility Loci
Polyploidy Enhances F Pollen Sterility Loci

... autotetraploid rice, we developed an autotetraploid rice hybrid that was heterozygous (SiSj) at F1 pollen sterility loci (Sa, Sb, and Sc) using near-isogenic lines. Cytological studies showed that the autotetraploid had higher percentages (.30%) of abnormal chromosome behavior and aberrant meiocytes ...
A systemic gene silencing method suitable for high throughput
A systemic gene silencing method suitable for high throughput

... molecules that mediate the degradation of complementary homologous RNA. Genetic screens in Arabidopsis thaliana, Caenorhabditis elegans and Neurospora crassa have identified homologous genes required for gene silencing [17-20], indicating that they share a common and evolutionarily conserved mechani ...
4 Conjugation in E. coli
4 Conjugation in E. coli

... Hfr-cell traslocates it’s genes in certain order at the same time the chromosome is replicated. Replication and translocation starts in certain ‘set-point’ in the integrated F-factor and can continue until the whole chromosome has been transferred, and the F-factor amongst it. The translocation of t ...
MyTaxa: an advanced taxonomic classifier for genomic and
MyTaxa: an advanced taxonomic classifier for genomic and

... 16S rRNA gene also provides limited resolution at the species level, which represents a major limitation for epidemiological and micro-diversity studies (11). To overcome these limitations, whole-genome-based approaches and tools, comparable to those already available for the 16S rRNA gene, are high ...
No Credible Scientific Evidence is Presented to Support Claims that
No Credible Scientific Evidence is Presented to Support Claims that

... show that not all CaMV positive samples were positive for the nos terminator. The absence of the nos terminator in some samples further casts doubt on the presence of intact, functional genes, which would have been expected if they were of transgenic plant origin. Furthermore, the lack of intact fun ...
Genetic epidemiology: Systemic lupus erythematosus | Arthritis
Genetic epidemiology: Systemic lupus erythematosus | Arthritis

... haplotype has been associated with SLE in whites and confers a two- to threefold increased risk of SLE [1]. The TNF-α –308A polymorphism is located within the promoter region of the gene and is associated with increased production of TNF-α. This polymorphism is in strong linkage disequilibrium with ...
The genome organisation of vertebrates
The genome organisation of vertebrates

... figures in the lower range being supported by recent results. If coding sequences average 1,000 bp, they would represent about 1% of the human genome, 99% or so of which is, therefore, made up of non-coding sequences (see Table 1). It should be noted that the larger number of genes in humans (and eu ...
Identification of Genes Needed for Regeneration, Stem Cell
Identification of Genes Needed for Regeneration, Stem Cell

... study of gene function in homeostasis, neoblast proliferation, and blastema differentiation and patterning allowed grouping of genes into functional categories. Together, our data present a broad survey of the genetic control of planarian biology and identify genes with ...
Lab 08-Flower development
Lab 08-Flower development

... The peloric mutant of the plant displayed has very similar characteristics to the snapdragon cyc dich double mutants. But rather than being due to changes in the genes themselves, the radialization of Linaria it is due to modifications of the DNA by addition of methyl groups (methylation) that inact ...
An Interaction-Dependent Model for Transcription Factor Binding
An Interaction-Dependent Model for Transcription Factor Binding

... could use to validate our findings, so we instead used the following approach to indirectly test our findings. If TF x antagonizes TF y, then for all the promoters which contain a motif for y, we should see an inverse correlation between the presence of motif x and the binding of TF y. In other word ...
Successive Losses of Central Immune Genes
Successive Losses of Central Immune Genes

... rerio (supplementary table S1, Supplementary Material online). Mx was identified in 38 of the 66 species sequenced by Malmstrøm et al. (2016). Of these 38, it was possible to obtain partial local gene synteny for 15 species, all of which share the same Mx-containing genomic region (supplementary tab ...
Brooker Chapter 4
Brooker Chapter 4

... Therefore, dark fur will only occur in cooler areas of the body ...
Genetic evaluation with major genes and polygenic
Genetic evaluation with major genes and polygenic

... use pedigree, genotype and phenotype information when some individuals are not genotyped. We propose to consider gene content at the major gene as a second trait correlated to the quantitative trait, in a gene content multipletrait best linear unbiased prediction (GCMTBLUP) method. Results:  The gen ...
DROSOPHILA MELANOGASTER - THE MODEL ORGANISM OF
DROSOPHILA MELANOGASTER - THE MODEL ORGANISM OF

... All biological processes are based on the use of a selected set of proteins to perform the many steps that underlie the final coherent set of events. It is an implicit assumption of modern biology that every activity of every organism can be viewed in this way. Given this recognition, two fundamenta ...
DNA methylation profile in human CD4+ T cells identifies
DNA methylation profile in human CD4+ T cells identifies

... CD4+ T cells. There was a significant difference in the average distance between the center of methylation peaks and the transcription start sites of methylated genes that are expressed compared to non-expressed genes. The center of methylation peaks was on average 449bp further upstream from the t ...
Genetics Power Point
Genetics Power Point

... – The farther apart two genes are located on a chromosome, the more likely they are to be separated by crossing over. – Genes located close together on a chromosome tend to be inherited together, which is called genetic linkage. • Genetic linkage allows the distance between two genes to ...
SUPPLEMENTARY INFORMATION TABLE OF CONTENTS: 1
SUPPLEMENTARY INFORMATION TABLE OF CONTENTS: 1

... corresponded to the ones previously identified in T. brucei, L. major and T. cruzi. In spite of the newly acquired Asn and Ser tRNAs, most of the tRNA genes are syntenic between both Phytomonas sequenced isolates. As shown in Figure S7, tRNA genes in HART1 scaffold 4 are syntenic with EM1 scaffold 4 ...
A Study of Gene Linkage and Mapping Using Tetrad Analysis
A Study of Gene Linkage and Mapping Using Tetrad Analysis

... could use the same approach to develop detailed predictions for the linked model (with genes on opposite or on the same side of the centromere). All models presented here consider only single crossovers. The effect of multiple crossovers and the use of mapping functions and the Poisson distribution ...
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.

... actl-2/actl-3) were tested at 37°C, growth was either extremely slow or absent, indicating that no significant complementation occurs among the three act) alleles and thus formally placing them in the same complementation group. To establish that this single complementation group represents the sing ...
14-2 Human Chromosomes
14-2 Human Chromosomes

... Autosomal vs Sex Linked Traits • Autosomes – All the chromosomes that do NOT play a role in sex determination • In humans, chromosomes 1 – 22 – Two copies » One from Mom » One from Dad » Same genes » Same location on chromosome » BUT may be different alleles (recipes) ...
Solid Tumour Section Soft Tissue Tumors: Low grade fibromyxoid sarcoma
Solid Tumour Section Soft Tissue Tumors: Low grade fibromyxoid sarcoma

... BAC clones were performed in two cases of low grade fibromyxoid sarcoma carrying a t(7;16) abnormality. The analysis revealed that the breakpoints were located within BAC clones RP11-388M20 (AC009088) in band 16p11.2, and RP11- 29B3 (AC022173) and RP11377B19 (AC009263) in band 7q33; all the examined ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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