Comparison with previous screens
... mitotic phenotypes including abnormalities in chromosome structure and integrity. The screen most similar to our own is the recent genome-wide survey performed by Goshima and coworkers [5]. They conducted their RNAi screen in the presence of dsRNA to Cdc27 (that encodes a subunit of the APC/C) to in ...
... mitotic phenotypes including abnormalities in chromosome structure and integrity. The screen most similar to our own is the recent genome-wide survey performed by Goshima and coworkers [5]. They conducted their RNAi screen in the presence of dsRNA to Cdc27 (that encodes a subunit of the APC/C) to in ...
in PDF format
... needs of euglenoids because they all require vitamins B1 and B12, which are released by the activity of associated microbial floras. ...
... needs of euglenoids because they all require vitamins B1 and B12, which are released by the activity of associated microbial floras. ...
Centriole Duplication: Centrin in on Answers? Dispatch
... pole body, lacks centrioles and forms a structure quite unlike that of the centrosome (reviewed in [5]). The morphological differences between centrosomes and spindle pole bodies are reflected in compositional differences. While the protein composition of the spindle pole body has been characterized ...
... pole body, lacks centrioles and forms a structure quite unlike that of the centrosome (reviewed in [5]). The morphological differences between centrosomes and spindle pole bodies are reflected in compositional differences. While the protein composition of the spindle pole body has been characterized ...
Assembly of the phragmoplast microtubule array in plant cells Bo Liu
... walls, from the interior to the periphery of the cell. These dynamic events are supported by a microtubule (MT)‐based structure, which is known as a phragmoplast. The phragmoplast is centrifugally expanded, which appears to be mediated by MT turnover involving the depolymerization of MTs and poly ...
... walls, from the interior to the periphery of the cell. These dynamic events are supported by a microtubule (MT)‐based structure, which is known as a phragmoplast. The phragmoplast is centrifugally expanded, which appears to be mediated by MT turnover involving the depolymerization of MTs and poly ...
Cell cycle control by ubiquitylation
... CKIs, negative-regulators of cyclin–CDK kinase complexes, are also targeted for degradation by the UPS. ...
... CKIs, negative-regulators of cyclin–CDK kinase complexes, are also targeted for degradation by the UPS. ...
Mitosis Online
... Take the quiz. Notice that when you click on an answer, you will be told whether you are correct or not (at the top of the page). There are a few of the questions that you have not learned about yet, but you might still be able to figure them out. ...
... Take the quiz. Notice that when you click on an answer, you will be told whether you are correct or not (at the top of the page). There are a few of the questions that you have not learned about yet, but you might still be able to figure them out. ...
Understanding Your Karyotype
... First we need to understand meiosis. Meiosis is the cell division process that produces egg and sperm cells (gametes), which normally have 23 chromosomes each. Play Meiosis animation here: http://learn.genetics.utah.edu/content/begin/tour/mitosis.swf ...
... First we need to understand meiosis. Meiosis is the cell division process that produces egg and sperm cells (gametes), which normally have 23 chromosomes each. Play Meiosis animation here: http://learn.genetics.utah.edu/content/begin/tour/mitosis.swf ...
Identification of a Mid-anaphase Checkpoint in
... mounting medium. Images were acquired and stored for further analysis, as described for FISH. Fluorescent digital images were analyzed with distance measuring tools within Metamorph software (Salmon et al., 1994). To visualize the nucleus, cells were fixed in 3.7% formaldehyde for 1 h at 258C. Fixed ...
... mounting medium. Images were acquired and stored for further analysis, as described for FISH. Fluorescent digital images were analyzed with distance measuring tools within Metamorph software (Salmon et al., 1994). To visualize the nucleus, cells were fixed in 3.7% formaldehyde for 1 h at 258C. Fixed ...
Chapter 12. Regulation of the Cell Cycle
... • Cyclins: proteins which attach to kinases to activate them; levels fluctuate in the cell cycle • When are cyclin levels highest? AP Biology ...
... • Cyclins: proteins which attach to kinases to activate them; levels fluctuate in the cell cycle • When are cyclin levels highest? AP Biology ...
Chapter 12 Lecture notes
... How do the kinetochore microtubules function in the poleward movement of chromosomes? ○ Two mechanisms are in play, both involving motor proteins. ○ Gary Borisy, of the University of Wisconsin, suggests that motor proteins on the kinetochores “walk” the chromosomes along the microtubules, which depo ...
... How do the kinetochore microtubules function in the poleward movement of chromosomes? ○ Two mechanisms are in play, both involving motor proteins. ○ Gary Borisy, of the University of Wisconsin, suggests that motor proteins on the kinetochores “walk” the chromosomes along the microtubules, which depo ...
patriciazuk.com
... the cell cycle • internal signal – e.g. kinetochores not attached to spindle microtubules send a molecular signal that delays anaphase – all chromosomes must be attached to the spindle in order to eventually activate and enzyme called separase – separase breaks down the cohesin proteins within the c ...
... the cell cycle • internal signal – e.g. kinetochores not attached to spindle microtubules send a molecular signal that delays anaphase – all chromosomes must be attached to the spindle in order to eventually activate and enzyme called separase – separase breaks down the cohesin proteins within the c ...
Cell Reproduction___notes outline cell cycle mitosis
... each stage, how many cells are present and the chromosome number (n, 2n, 4n) present at each stage for each cell interphase – 3?s draw & label diagram w/ following parts: cell membrane, nuclear envelope, centriole, chromatin or chromatids mitosis – 3?s cytokinesis – 3?s draw & label animal ...
... each stage, how many cells are present and the chromosome number (n, 2n, 4n) present at each stage for each cell interphase – 3?s draw & label diagram w/ following parts: cell membrane, nuclear envelope, centriole, chromatin or chromatids mitosis – 3?s cytokinesis – 3?s draw & label animal ...
01 Chapter 7 Reading Guide - Student
... 10. Explain the following regions of the prokaryotic chromosome with respect to their role in binary fission: a) ori – b) ter – ...
... 10. Explain the following regions of the prokaryotic chromosome with respect to their role in binary fission: a) ori – b) ter – ...
doc bio notes
... there’s one naked kinetochore, cdc20 can’t be activated. Spindle assembly checkpoint: system reading state of the spindle. As long as even one chromosome that doesn’t have kinetochore occupied by microtubules, mitosis can not continue. The reason why it can’t continue, cdc20 can’t bind to APC. An un ...
... there’s one naked kinetochore, cdc20 can’t be activated. Spindle assembly checkpoint: system reading state of the spindle. As long as even one chromosome that doesn’t have kinetochore occupied by microtubules, mitosis can not continue. The reason why it can’t continue, cdc20 can’t bind to APC. An un ...
mock exam 2 - answers
... 35. Which of the following describes a difference between spermatogenesis and oogenesis? a. Spermatogenesis produces four viable gametes, while oogenesis produces one gamete b. Spermatogenesis is interrupted multiple times, whereas oogenesis is continuous c. Spermatogenesis does not occur throughout ...
... 35. Which of the following describes a difference between spermatogenesis and oogenesis? a. Spermatogenesis produces four viable gametes, while oogenesis produces one gamete b. Spermatogenesis is interrupted multiple times, whereas oogenesis is continuous c. Spermatogenesis does not occur throughout ...
repp86: A Human Protein Associated in the Progression of Mitosis
... domain cosediment with polymerized microtubules, linking repp86 to the family of microtubule-associated proteins. During prophase and metaphase, repp86 interacts on the mitotic spindle with the putative motor protein Hklp2. Thus, repp86 may function in targeting Hklp2 to the microtubule minus ends, ...
... domain cosediment with polymerized microtubules, linking repp86 to the family of microtubule-associated proteins. During prophase and metaphase, repp86 interacts on the mitotic spindle with the putative motor protein Hklp2. Thus, repp86 may function in targeting Hklp2 to the microtubule minus ends, ...
cell division: binary fission and mitosis
... segregation of the chromosomes and organelles into daughter cells. Click here to view an animated GIF of mitosis from http://www.biology.uc.edu/vgenetic/mitosis/mitosis.htm. ...
... segregation of the chromosomes and organelles into daughter cells. Click here to view an animated GIF of mitosis from http://www.biology.uc.edu/vgenetic/mitosis/mitosis.htm. ...
Mitosis - RuthenbergAP
... from the Golgi coalesce at the metaphase plate, forming a cell plate. • The plate enlarges until its membranes fuse with the plasma membrane at the perimeter, with the contents of the vesicles forming new wall material in between. Fig. 12.8b Copyright © 2002 Pearson Education, Inc., publishing as Be ...
... from the Golgi coalesce at the metaphase plate, forming a cell plate. • The plate enlarges until its membranes fuse with the plasma membrane at the perimeter, with the contents of the vesicles forming new wall material in between. Fig. 12.8b Copyright © 2002 Pearson Education, Inc., publishing as Be ...
Centromere dynamics
... histone variants that are conserved throughout their length relative to the canonical histone, CenH3 is only 50% identical to histone H3 over the shared histone-fold domain (HFD; 100 amino acids) [7]. At the N terminus, there is a divergent helix (N-helix; 50aa) that in budding yeasts (Saccharomyc ...
... histone variants that are conserved throughout their length relative to the canonical histone, CenH3 is only 50% identical to histone H3 over the shared histone-fold domain (HFD; 100 amino acids) [7]. At the N terminus, there is a divergent helix (N-helix; 50aa) that in budding yeasts (Saccharomyc ...
Spindle checkpoint
During the process of cell division, the spindle checkpoint prevents separation of the duplicated chromosomes until each chromosome is properly attached to the spindle apparatus. In order to preserve the cell's identity and proper function, it is necessary to maintain the appropriate number of chromosomes after each cell division. An error in generating daughter cells with fewer or greater number of chromosomes than expected (a situation termed aneuploidy), may lead in best case to cell death, or alternatively it may generate catastrophic phenotypic results. Examples include: In cancer cells, aneuploidy is a frequent event, indicating that these cells present a defect in the machinery involved in chromosome segregation, as well as in the mechanism ensuring that segregation is correctly performed. In humans, Down syndrome appears in children carrying in their cells one extra copy of chromosome 21, as a result of a defect in chromosome segregation during meiosis in one of the progenitors. This defect will generate a gamete (spermatozoide or oocyte) with an extra chromosome 21. After fecundation, this gamete will generate an embryo with three copies of chromosome 21.The mechanisms verifying that all the requirements to pass to the next phase in the cell cycle have been fulfilled are called checkpoints. All along the cell cycle, there are different checkpoints. The checkpoint ensuring that chromosome segregation is correct is termed spindle assembly checkpoint (SAC), spindle checkpoint or mitotic checkpoint. During mitosis or meiosis, the spindle checkpoint prevents anaphase onset until all chromosomes are properly attached to the spindle. To achieve proper segregation, the two kinetochores on the sister chromatids must be attached to opposite spindle poles (bipolar orientation). Only this pattern of attachment will ensure that each daughter cell receives one copy of the chromosome.