Poster
... to thousands of humans annually. TeNT is produced by the bacterium, Clostridium tetani, an anaerobic bacterium usually found as spores in soil. C. tetani often infects humans through open wounds where the bacterium colonizes the infected tissues. There are two domains of TeNT: the A domain possesses ...
... to thousands of humans annually. TeNT is produced by the bacterium, Clostridium tetani, an anaerobic bacterium usually found as spores in soil. C. tetani often infects humans through open wounds where the bacterium colonizes the infected tissues. There are two domains of TeNT: the A domain possesses ...
(GCKIII) proteins using a mechanism analogous to CCM3
... C-terminal region of the GCKIII proteins is a key determinant for CCM3 recognition. The C-terminal tails of MST4 and STK25, when expressed as GST fusion proteins, were sufficient for robust binding to full length CCM3 (Fig. 1C). Interestingly, neither GST kinase tail fusion was able to bind to the i ...
... C-terminal region of the GCKIII proteins is a key determinant for CCM3 recognition. The C-terminal tails of MST4 and STK25, when expressed as GST fusion proteins, were sufficient for robust binding to full length CCM3 (Fig. 1C). Interestingly, neither GST kinase tail fusion was able to bind to the i ...
coIP就是co-immunoprecipitation,假如有两种蛋白质A,B相互作用
... during the assay. Since transient interactions occur during transport or as part of enzymatic processes, they often require cofactors and energy via nucleotide triphosphates hydrolysis. Incorporating cofactors and nonhydrolyzable NTP analogs during assay optimization can serve to ‘trap’ interacting ...
... during the assay. Since transient interactions occur during transport or as part of enzymatic processes, they often require cofactors and energy via nucleotide triphosphates hydrolysis. Incorporating cofactors and nonhydrolyzable NTP analogs during assay optimization can serve to ‘trap’ interacting ...
Longins and their longin domains: regulated SNAREs and
... LD of Sec22, unlike that of Ykt6, does not fold back and interact with its SNARE motif to adopt a closed conformation [19]. It remains, however, to be established whether Sec22 can adopt such a conformation during its incorporation into coat protein II (COPII)-coated vesicles [21,57], and whether th ...
... LD of Sec22, unlike that of Ykt6, does not fold back and interact with its SNARE motif to adopt a closed conformation [19]. It remains, however, to be established whether Sec22 can adopt such a conformation during its incorporation into coat protein II (COPII)-coated vesicles [21,57], and whether th ...
interpreted as a demonstration of a biologically significant protein
... the [32p]EBNA1PK and both N- and C-labeled [32p]croPK retained their sitespecific D N A binding activities. It is important to note that if the amount of ATP used in the labeling reaction is substoichiometric to the protein (often the case in D N A end-labeling reactions), some types of activity ass ...
... the [32p]EBNA1PK and both N- and C-labeled [32p]croPK retained their sitespecific D N A binding activities. It is important to note that if the amount of ATP used in the labeling reaction is substoichiometric to the protein (often the case in D N A end-labeling reactions), some types of activity ass ...
The split Grainyhead protein of Cnidaria Grainyhead
... Nine motifs were shared between GRH and LSF proteins. Two were unique to LSF. Three were unique to GRH. Based on the motif architecture, the basal metazoan sequences appeared to be bona fide GRH proteins Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to dis ...
... Nine motifs were shared between GRH and LSF proteins. Two were unique to LSF. Three were unique to GRH. Based on the motif architecture, the basal metazoan sequences appeared to be bona fide GRH proteins Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to dis ...
`Meta` Approaches to Protein Structure Prediction
... last few years. A large number of fully automated servers, covering various aspects of structure prediction, are currently available to the scientific community. In addition to the biannual Critical Assessment of Structure Prediction (CASP) experiment, which evaluates the state-of-the-art in the met ...
... last few years. A large number of fully automated servers, covering various aspects of structure prediction, are currently available to the scientific community. In addition to the biannual Critical Assessment of Structure Prediction (CASP) experiment, which evaluates the state-of-the-art in the met ...
Bending membranes
... for the budding of vesicular and tubular carriers of intracellular cargo. This mechanism has a role in the formation of most intracellular vesicular carriers, particularly those associated with clathrin or the COPI or COPII coat protein complexes. The discovery of membrane-associated proteins with c ...
... for the budding of vesicular and tubular carriers of intracellular cargo. This mechanism has a role in the formation of most intracellular vesicular carriers, particularly those associated with clathrin or the COPI or COPII coat protein complexes. The discovery of membrane-associated proteins with c ...
Chaperone-assisted protein folding: the path to discovery from a
... binding and unbinding in a mechanism regulated by the GroEL ATPase24. Importantly, Jörg Martin found that GroES can bind the same ring that holds the unfolded substrate protein. Suddenly, the pieces of the puzzle began to fall into place, and we became increasingly confident that GroEL and GroES ess ...
... binding and unbinding in a mechanism regulated by the GroEL ATPase24. Importantly, Jörg Martin found that GroES can bind the same ring that holds the unfolded substrate protein. Suddenly, the pieces of the puzzle began to fall into place, and we became increasingly confident that GroEL and GroES ess ...
PHD domains and E3 ubiquitin ligases: viruses make the connection
... receptor cytosolic tail, triggering endocytosis and, ultimately, delivery of the endosomal contents to the lysosome. Notably, in yeast the internalization of many different proteins and the subsequent delivery of their endosomes to the lysosome-like vacuole are steps that are also controlled by ubiq ...
... receptor cytosolic tail, triggering endocytosis and, ultimately, delivery of the endosomal contents to the lysosome. Notably, in yeast the internalization of many different proteins and the subsequent delivery of their endosomes to the lysosome-like vacuole are steps that are also controlled by ubiq ...
Protein synthesis I Biochemistry 302 February 17, 2006
... Under the ionic conditions present in the cell, ribosomes exist primarily as dissociated subunits. ...
... Under the ionic conditions present in the cell, ribosomes exist primarily as dissociated subunits. ...
Biocatalytic Synthesis of Polymers of Precisely Defined Structures
... more complex globular counterparts. In addition, these polypeptides also adopt regular and persistent secondary structures in both the solution and solid states, consistent with the geometrical requirements of their oligopeptide repeats. By utilizing the principles of protein structure and the conce ...
... more complex globular counterparts. In addition, these polypeptides also adopt regular and persistent secondary structures in both the solution and solid states, consistent with the geometrical requirements of their oligopeptide repeats. By utilizing the principles of protein structure and the conce ...
Protein Quality Control Mechanisms and Protein
... Mainieri et al., 2004). These protein bodies sequester a proportion of BiP, which can be released by ATP in vitro treatment, indicating that BiP is not trapped but is acting as a chaperone (heat shock-70 chaperones are ATPases and use ATP hydrolysis to perform their function). Zeolin protein bodies ...
... Mainieri et al., 2004). These protein bodies sequester a proportion of BiP, which can be released by ATP in vitro treatment, indicating that BiP is not trapped but is acting as a chaperone (heat shock-70 chaperones are ATPases and use ATP hydrolysis to perform their function). Zeolin protein bodies ...
What`s in YOUR protein bar?
... In the world of “health” bars there are so many to choose from that it’s easy to get confused. There are bars to replace energy, some to lose weight, some build and repair muscle and if you are just blindly eating whatever is FDA approved you could be eating some pretty strange things that you would ...
... In the world of “health” bars there are so many to choose from that it’s easy to get confused. There are bars to replace energy, some to lose weight, some build and repair muscle and if you are just blindly eating whatever is FDA approved you could be eating some pretty strange things that you would ...
Basic Structure of Proteins
... How many structures does a protein have? What is the primary structure of protein? Which is the biological importance of the primary structure of proteins? Which is the stabilizing chemical bond of primary structure of proteins? What is the secondary structure of proteins? Which is the stabilizing c ...
... How many structures does a protein have? What is the primary structure of protein? Which is the biological importance of the primary structure of proteins? Which is the stabilizing chemical bond of primary structure of proteins? What is the secondary structure of proteins? Which is the stabilizing c ...
Sourirajan, Jayanthi: Protein Structure Prediction
... interactions, study of evolutionary relationship between proteins or protein families etc. The 3D structure of proteins can be solved by 1) Experimental methods, or 2) Structure prediction. Solving structures experimentally is very hard. Solving through X-ray crystallography produces very good resul ...
... interactions, study of evolutionary relationship between proteins or protein families etc. The 3D structure of proteins can be solved by 1) Experimental methods, or 2) Structure prediction. Solving structures experimentally is very hard. Solving through X-ray crystallography produces very good resul ...
Eukaryotic Transcription factors: the DNA binding domain
... The amphipathic helix-loop-helix (HLH) motif was identified in certain transcription factors involved in developmental regulation. They contain a stretch of 40-50 aminoacids comprising of two amphipathic a-helices separated by a linker region (the loop) of varying length. The HLH proteins form both ...
... The amphipathic helix-loop-helix (HLH) motif was identified in certain transcription factors involved in developmental regulation. They contain a stretch of 40-50 aminoacids comprising of two amphipathic a-helices separated by a linker region (the loop) of varying length. The HLH proteins form both ...
Serum Protein Electrophoresis – What is it
... Unexplained proteinuria, overflow type When not to order serum protein electrophoresis: Although serum protein electrophoresis can show different patterns in many diseases including liver disease, chronic inflammation, acute infections, autoimmune diseases etc. this test modality should not be used ...
... Unexplained proteinuria, overflow type When not to order serum protein electrophoresis: Although serum protein electrophoresis can show different patterns in many diseases including liver disease, chronic inflammation, acute infections, autoimmune diseases etc. this test modality should not be used ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.