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Dynamics of transcription and mRNA export
Dynamics of transcription and mRNA export

... kinetic analyses at the single gene level will resolve these issues. To follow the activity of specific genes in single living cells, cell lines have been generated where a particular gene is integrated as multiple tandem repeats in the genome (Figure 1) and can be visualized either using the lac op ...
File - Integrated Science
File - Integrated Science

... Targeted deletion by homologous recombination (KNOCKOUT MICE) - We’ll discuss next time  Specific mutational changes can be made  Time consuming and limited to certain organisms  Antisense RNA  Variable effects and mechanism not understood ...
unit 5 study guide (ch 13-15)
unit 5 study guide (ch 13-15)

Study Guide for Test on Chapter 11 and 14-1, 14-2
Study Guide for Test on Chapter 11 and 14-1, 14-2

... o Describe what happens during each phase of meiosis I and meiosis II  Focus on what happens to the chromosomes  Know when tetrad formation and crossing-over occurs o Distinguish among the type and number of gametes formed in males vs. in females  Know how many sperm, eggs, and polar bodies are f ...
Lecture 1: Introduction. Gametogenesis. Fertilization.
Lecture 1: Introduction. Gametogenesis. Fertilization.

... − changes in gene expression (gene in/activation), DNA methylation, chemical modification of nuclear histones, non-coding RNA with regulation function, pre-mRNA splicing, posttranslational modification of proteins − cell-to-cell contacts, determination of cellular position, chemical gradients − radi ...
Supplementary Material Deep sequencing of the human
Supplementary Material Deep sequencing of the human

... electrophoresis of amplicons from the Retina2 cDNA (+RT) and the no reverse transcriptase controls (-RT) that exclude the possibility of genomic DNA contamination. The PCR results were verified by Sanger sequencing. In some instances, the primers amplified fragments that could originate from two hig ...
What is a Virus? - columbusisd.org
What is a Virus? - columbusisd.org

...  Composite Transposons are more complex than insertion sequences containing multiple genes sandwiched between the insertion sequences.  Generate genetic diversity by moving genes from one chromosome to another, or another species. ...
A Basic Introduction to the Science Underlying NCBI Resources
A Basic Introduction to the Science Underlying NCBI Resources

... genes. Plants also have a second organelle, the chloroplast, which also has its own DNA. Cells often have multiple mitochondria, particularly cells requiring lots of energy, such as active muscle cells. This is because mitochondria are responsible for converting the energy stored in macromolecules i ...
Chapter 7 – Recombination in Bacteria and
Chapter 7 – Recombination in Bacteria and

... bacterial cell wall - recombination leads to integration - transformation can also be induced in plant and animal cells - the frequency of bacterial transformation can be increased by manipulating [Ca+2] and electric shock (a treated cell is said to be COMPETENT to take up DNA) Linkage Information a ...
CSE 181 Project guidelines
CSE 181 Project guidelines

... http://www.cgl.ucsf.edu/home/glasfeld/tutorial/trna/trna.gif ...
Notes
Notes

... square, we simply need to match up each combination of letters to create the possible genotypes that could be created by this combination of parents.  Remember to keep similar letters together and to write capital letters first. ...
Gene Technology Quest – Study Guide KEY What is a genome? A
Gene Technology Quest – Study Guide KEY What is a genome? A

... Sticky ends are the overhang of nucleotides that result when a restriction enzyme cuts DNA. Their importance is that this allows for DNA from other organisms to join this genome in order to make recombinant DNA. 9. How is recombinant DNA formed? Recombinant DNA is formed when a restriction enzyme cu ...
Gene Section FLI1 (Friend leukemia virus integration 1) in Oncology and Haematology
Gene Section FLI1 (Friend leukemia virus integration 1) in Oncology and Haematology

... The fli-1 gene encodes two isoforms of 51 and 48 kDa, synthesized by alternative translation initiation sites, as mentioned above. Loss of function studies have provided evidence to suggest that both the p51 and p48 isoforms retain the same functional domains and activity (Melet et al., 1996). The f ...
chapteroutline_ch07
chapteroutline_ch07

... 7.9 Incomplete dominance and codominance: The effects of both alleles in a genotype can show up in the phenotype. TAKE-HOME MESSAGE 7.9: Sometimes the effects of both alleles in a heterozygous genotype are evident in the phenotype. With incomplete dominance, the phenotype of a heterozygote appears t ...
Statistical analysis of DNA microarray data
Statistical analysis of DNA microarray data

... Figure 2. Genome-wide Location of the Nine Cell Cycle Transcription Factors(A) 213 of the 800 cell cycle genes whose promoter regions were bound by a myc-tagged version of at least one of the nine cell cycle transcription factors (p < 0.001) are represented as horizontal lines. The weight-averaged b ...
Gene technology
Gene technology

... map of the sheep being one of the most accurate and comprehensive available, with about 1500 markers. These gene maps are essential tools to locate individual genes (markers) which affect traits of economic importance such as fibre diameter or disease resistance. Gene maps will help researchers bett ...
Lecture 8-Neoplasia 2
Lecture 8-Neoplasia 2

... • When RB is phosphorylated by the cyclin D-CDK4/6 and cyclin E-CDK2 complexes, it releases E2F. • E2F then activates transcription of S-phase genes. • The phosphorylation of RB is inhibited by CDKIs, because they inactivate cyclin-CDK complexes Hypophosphorylated RB in complex with the E2F transcri ...
MENDELIAN INHERITANCE
MENDELIAN INHERITANCE

... • XIST: X-inactive-specific transcript • XIST produces a non-coding 17 kb RNA molecule • “Coats” the entire local X-chromosome – cis-acting ...
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... the inheritance of other genes that are difficult to identify. • The marker gene must be located on the same chromosome and, ideally, at a very small distance from the gene being traced (to avoid crossing over). ...
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... • R (resistance) Plasmid: codes for antibiotic drug resistance ...
Lecture_12_2005
Lecture_12_2005

... – Many times fusions will not be functional. ...
Leukaemia Section t(3;11)(q26;p15) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(3;11)(q26;p15) Atlas of Genetics and Cytogenetics in Oncology and Haematology

Exam 1
Exam 1

... e. None of the above ...
dragon reading
dragon reading

... connected males have copies of the same Y chromosome, and thus share any Y-linked characteristics. One final note about that very significant white-eyed Drosophila gene. In combination with a strange chromosomal anomaly called attached-X, this gene also produced the first direct evidence that genes ...
PattArAn – From Annotation Triplets to Sentence Fingerprints
PattArAn – From Annotation Triplets to Sentence Fingerprints

... Document 17028151 indicates that upon infection with Pseudomonas syringae, expression levels drop significantly in Arabidopsis leaves. This process is one aspect of a complex, genome wide response to bacterial infection involving many genes.  Inferred Triplet: Using doublets in document (18305484) ...
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Epigenetics of human development

Development before birth, including gametogenesis, embryogenesis, and fetal development, is the process of body development from the gametes are formed to eventually combine into a zygote to when the fully developed organism exits the uterus. Epigenetic processes are vital to fetal development due to the need to differentiate from a single cell to a variety of cell types that are arranged in such a way to produce cohesive tissues, organs, and systems.Epigenetic modifications such as methylation of CpGs (a dinucleotide composed of a 2'-deoxycytosine and a 2' deoxyguanosine) and histone tail modifications allow activation or repression of certain genes within a cell, in order to create cell memory either in favor of using a gene or not using a gene. These modifications can either originate from the parental DNA, or can be added to the gene by various proteins and can contribute to differentiation. Processes that alter the epigenetic profile of a gene include production of activating or repressing protein complexes, usage of non-coding RNAs to guide proteins capable of modification, and the proliferation of a signal by having protein complexes attract either another protein complex or more DNA in order to modify other locations in the gene.
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