![Quantitative expression of cholera toxin mRNA in Vibrio cholerae](http://s1.studyres.com/store/data/023588236_1-8ab697534804f6899c7e7e9ccac966b0-300x300.png)
Quantitative expression of cholera toxin mRNA in Vibrio cholerae
... tified using SYBR Green I dye. The amount of cDNA used for qPCR from each sample was 50 ng ml21. Four primers were designed: recA-f (59-ATTGAAGGCGAAATGGGCGATAG-39), recA-r (59-TACACATACAGTTGGATTGCTTGAG-39), ctxAB-f (59-TATGCCAAGAGGACAGAGTGAG-39) and ctxAB-r (59-AACATATCCATCATCGTGCCTAAC-39). In the p ...
... tified using SYBR Green I dye. The amount of cDNA used for qPCR from each sample was 50 ng ml21. Four primers were designed: recA-f (59-ATTGAAGGCGAAATGGGCGATAG-39), recA-r (59-TACACATACAGTTGGATTGCTTGAG-39), ctxAB-f (59-TATGCCAAGAGGACAGAGTGAG-39) and ctxAB-r (59-AACATATCCATCATCGTGCCTAAC-39). In the p ...
5-Dihybrids Notes
... A monohybrid cross involves only one trait. This is what we have been looking at so far. Ex. Only dimples, OR only tongue rolling ability. A dihybrid cross involves two traits. Also known as two-factor cross. Ex. dimples AND tongue rolling ability. ...
... A monohybrid cross involves only one trait. This is what we have been looking at so far. Ex. Only dimples, OR only tongue rolling ability. A dihybrid cross involves two traits. Also known as two-factor cross. Ex. dimples AND tongue rolling ability. ...
Shoot Branching and Leaf Dissection in Tomato Are
... indicating that they originated by tandem duplication. However, the presence of at least five substitutions of highly conserved amino acid residues in the MYB domain indicates that Bli6 is most probably a pseudogene (see Supplemental Figure 1 online). This conclusion is corroborated by the phenotype ...
... indicating that they originated by tandem duplication. However, the presence of at least five substitutions of highly conserved amino acid residues in the MYB domain indicates that Bli6 is most probably a pseudogene (see Supplemental Figure 1 online). This conclusion is corroborated by the phenotype ...
The Frequency Distribution of Nucleotide Variation in Drosophila
... 0.11). This dependence of the statistical results on period data could be indicative of locus effects or could be attributable to reduced power associated with removal of a large amount of data from the analysis. The conclusion of roughly equal numbers of preferred and unpreferred fixations is based ...
... 0.11). This dependence of the statistical results on period data could be indicative of locus effects or could be attributable to reduced power associated with removal of a large amount of data from the analysis. The conclusion of roughly equal numbers of preferred and unpreferred fixations is based ...
HGSS2 Mendel
... Finally, we also know that not all hereditary factors assort independently. Those that are located close together on the same chromosome tend to be inherited as a unit, not as independent entities. These exceptions, however, are individual trees within the forest. Mendel’s great accomplishment was t ...
... Finally, we also know that not all hereditary factors assort independently. Those that are located close together on the same chromosome tend to be inherited as a unit, not as independent entities. These exceptions, however, are individual trees within the forest. Mendel’s great accomplishment was t ...
Applet for calculating heritability for threshold traits
... milk, as shown in section 1.4. To make this possible there has to be an effect from numerous gene pairs. In the present example using only two gene pairs, they could be fixed after one generation of selection. ...
... milk, as shown in section 1.4. To make this possible there has to be an effect from numerous gene pairs. In the present example using only two gene pairs, they could be fixed after one generation of selection. ...
Interrogating the Function of Metazoan Histones using
... of these criteria. First, histone proteins have the potential to be dynamic regulators of genome activity because they are subject to a broad range of posttranslational modifications (PTMs), including phosphorylation, acetylation, and methylation (Rothbart and Strahl, 2014). Histone PTMs are thought ...
... of these criteria. First, histone proteins have the potential to be dynamic regulators of genome activity because they are subject to a broad range of posttranslational modifications (PTMs), including phosphorylation, acetylation, and methylation (Rothbart and Strahl, 2014). Histone PTMs are thought ...
Fulltext PDF - Indian Academy of Sciences
... Heritable information in plants consists of genomic information in DNA sequence and epigenetic information superimposed on DNA sequence. The latter is in the form of cytosine methylation at CG, CHG and CHH elements (where H = A, T or C) and a variety of histone modifications in nucleosomes. The epial ...
... Heritable information in plants consists of genomic information in DNA sequence and epigenetic information superimposed on DNA sequence. The latter is in the form of cytosine methylation at CG, CHG and CHH elements (where H = A, T or C) and a variety of histone modifications in nucleosomes. The epial ...
Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1a/tcf1
... 1999), intestine (Mitchelmore et al., 2000) and kidney (Pontoglio et al., 2000). Although mutations in human hnf1a gene have been shown to cause maturity-onset diabetes of the young, i.e., MODY3 (Yamagata et al., 1996), hnf1a does not seem to play an essential role during development (Pontoglio et a ...
... 1999), intestine (Mitchelmore et al., 2000) and kidney (Pontoglio et al., 2000). Although mutations in human hnf1a gene have been shown to cause maturity-onset diabetes of the young, i.e., MODY3 (Yamagata et al., 1996), hnf1a does not seem to play an essential role during development (Pontoglio et a ...
Efficient Ends-Out Gene Targeting In Drosophila
... although for long-term stock maintenance, we recommend 18ºC. Males of Y.hs-hid are slightly weaker due to having two copies of hs-hid genes, so culturing stocks under 25ºC is not recommended. It is important to keep vials and bottles from being overcrowded since Y.hs-hid males may not survive very w ...
... although for long-term stock maintenance, we recommend 18ºC. Males of Y.hs-hid are slightly weaker due to having two copies of hs-hid genes, so culturing stocks under 25ºC is not recommended. It is important to keep vials and bottles from being overcrowded since Y.hs-hid males may not survive very w ...
Methods of Human Heredity Study
... centromeres are constant, and are called constitutive heterochromatic regions serving as chromosome markers. There are other heterochromatic regions, called facultative heterochromatin and represented by whole sex chromosomes, which become heterochromatic only at certain stages. For instance, in fem ...
... centromeres are constant, and are called constitutive heterochromatic regions serving as chromosome markers. There are other heterochromatic regions, called facultative heterochromatin and represented by whole sex chromosomes, which become heterochromatic only at certain stages. For instance, in fem ...
Chapter 4: Individual gene function
... important caveat to using gene products (RNAs or proteins) as evidence that an allele is a null is that a gene could have activity at a low level of product that cannot be easily detected. It is possible that even when we cannot detect a gene product, there may be enough gene product present in the ...
... important caveat to using gene products (RNAs or proteins) as evidence that an allele is a null is that a gene could have activity at a low level of product that cannot be easily detected. It is possible that even when we cannot detect a gene product, there may be enough gene product present in the ...
presentation - Genome-to-Genome Distance Calculator
... 4045 ongoing bacterial genome sequencing projects (data from ...
... 4045 ongoing bacterial genome sequencing projects (data from ...
Supplemental Material
... chromosome arm but also to order and orient the scaffolds within the arm. Interestingly, these alignments were largely congruent with the older purely cytological observations confirming the basic accuracy of the earlier observations (ibid). For the more distantly related species, this was not possi ...
... chromosome arm but also to order and orient the scaffolds within the arm. Interestingly, these alignments were largely congruent with the older purely cytological observations confirming the basic accuracy of the earlier observations (ibid). For the more distantly related species, this was not possi ...
A complete shikimate pathway in Toxoplasma gondii: an ancient
... enzymes (Roberts et al., 1998). Having identified the genes that encode all seven steps of the shikimate pathway (Roberts et al., 1998 and the current paper) we were intrigued to investigate the evolutionary origins of these genes. Current sampling of shikimate pathway genes is confined primarily to ...
... enzymes (Roberts et al., 1998). Having identified the genes that encode all seven steps of the shikimate pathway (Roberts et al., 1998 and the current paper) we were intrigued to investigate the evolutionary origins of these genes. Current sampling of shikimate pathway genes is confined primarily to ...
1 Origins of Hereditary Science
... he taught high school and cared for a garden. It was in this garden that he completed his important experiments. Most of Mendel’s experiments involved crossing different types of pea plants. In this case, the word cross means “to mate or breed two individuals.” Mendel crossed a type of garden pea pl ...
... he taught high school and cared for a garden. It was in this garden that he completed his important experiments. Most of Mendel’s experiments involved crossing different types of pea plants. In this case, the word cross means “to mate or breed two individuals.” Mendel crossed a type of garden pea pl ...
Speciation in Drosophila: From Phenotypes to Molecules
... early 1990s. At that time, several experiments were performed that appeared to falsify, or at least to lessen the plausibility of, the idea (Johnson and Wu 1992; Coyne and Orr 1993). These experiments showed that species hybrids that were partially sterile—thus allowing recovery of some gametes—suff ...
... early 1990s. At that time, several experiments were performed that appeared to falsify, or at least to lessen the plausibility of, the idea (Johnson and Wu 1992; Coyne and Orr 1993). These experiments showed that species hybrids that were partially sterile—thus allowing recovery of some gametes—suff ...
Specification of unique neuronal sub-types by integration of positional and temporal cues
... myelin which surround, isolates and ensheaths neuronal circuits, making sure that action potentials are transported in a safe and undisturbed way. In mammals we find four types of glial cells: microglia, astrocytes, Schwann cells and oligdendrocytes each one with different functions within the CNS. ...
... myelin which surround, isolates and ensheaths neuronal circuits, making sure that action potentials are transported in a safe and undisturbed way. In mammals we find four types of glial cells: microglia, astrocytes, Schwann cells and oligdendrocytes each one with different functions within the CNS. ...
Lesson Overview
... During gamete formation, the alleles for each gene segregate from each other, so that each gamete carries only one allele for each gene. ...
... During gamete formation, the alleles for each gene segregate from each other, so that each gamete carries only one allele for each gene. ...
Speciation in Drosophila: From Phenotypes to Molecules
... early 1990s. At that time, several experiments were performed that appeared to falsify, or at least to lessen the plausibility of, the idea (Johnson and Wu 1992; Coyne and Orr 1993). These experiments showed that species hybrids that were partially sterile—thus allowing recovery of some gametes—suff ...
... early 1990s. At that time, several experiments were performed that appeared to falsify, or at least to lessen the plausibility of, the idea (Johnson and Wu 1992; Coyne and Orr 1993). These experiments showed that species hybrids that were partially sterile—thus allowing recovery of some gametes—suff ...
Testing the ABC floral-organ identity model
... In wild type plants, AP1 transcript is first observed throughout the stage 1 floral meristem but disappears from the centre of the stage 3 floral meristem. In later stages it continues to be expressed in developing sepals and petals. ...
... In wild type plants, AP1 transcript is first observed throughout the stage 1 floral meristem but disappears from the centre of the stage 3 floral meristem. In later stages it continues to be expressed in developing sepals and petals. ...
Document
... • A gamete (sperm or egg) contains a single set of chromosomes, and is haploid (n) • For humans, the haploid number is 23 (n = 23) • Each set of 23 consists of 22 autosomes and a single sex chromosome • In an unfertilized egg (ovum), the sex chromosome is X • In a sperm cell, the sex chromosome may ...
... • A gamete (sperm or egg) contains a single set of chromosomes, and is haploid (n) • For humans, the haploid number is 23 (n = 23) • Each set of 23 consists of 22 autosomes and a single sex chromosome • In an unfertilized egg (ovum), the sex chromosome is X • In a sperm cell, the sex chromosome may ...
MicroReview Paradigms of plasmid organization
... prompted by DNA damage and, for circular molecules, this is a problem because it can cause dimerization, which prevents separation of the monomers at partitioning. So, for both high- and low-copy-number plasmids, a multimer resolution system (mrs) that ensures that each copy of the plasmid genome fu ...
... prompted by DNA damage and, for circular molecules, this is a problem because it can cause dimerization, which prevents separation of the monomers at partitioning. So, for both high- and low-copy-number plasmids, a multimer resolution system (mrs) that ensures that each copy of the plasmid genome fu ...
Leukaemia Section ider(20q) in Myeloid Malignancies Atlas of Genetics and Cytogenetics
... Leukemia (5 cases), Chronic Myelomonocytic Leukemia (1 case). ...
... Leukemia (5 cases), Chronic Myelomonocytic Leukemia (1 case). ...