Atomic-Level Mapping of Antibody Epitopes by
... Shotgun Mutagenesis uses a proprietary high-throughput cell expression technology that enables the expression and analysis of large libraries of mutated target proteins within eukaryotic cells. Every residue in a protein is mutated, usually multiple times, in order to assay changes in function. Enti ...
... Shotgun Mutagenesis uses a proprietary high-throughput cell expression technology that enables the expression and analysis of large libraries of mutated target proteins within eukaryotic cells. Every residue in a protein is mutated, usually multiple times, in order to assay changes in function. Enti ...
Molecular Modeling: A Magical Tool for Drug Design and
... Docking, combined with other computational techniques and experimental data, also could be involved in analyzing drug metabolism to obtain some useful information from the cytochrome P450 system. Here example of successful applications of docking are presented. DNA gyrase is a bacterial enzyme that ...
... Docking, combined with other computational techniques and experimental data, also could be involved in analyzing drug metabolism to obtain some useful information from the cytochrome P450 system. Here example of successful applications of docking are presented. DNA gyrase is a bacterial enzyme that ...
Document
... Regulation is accomplished by sequence-specific DNA binding proteins. Binding may be promoter proximal or at a distance. DNA footprinting and mobility shift assays are used to investigate the binding of regulatory proteins. In the E. coli lac operon, there are both repressors and activators, each of ...
... Regulation is accomplished by sequence-specific DNA binding proteins. Binding may be promoter proximal or at a distance. DNA footprinting and mobility shift assays are used to investigate the binding of regulatory proteins. In the E. coli lac operon, there are both repressors and activators, each of ...
Enzymes: Principles of Catalysis
... 1. Regulation by Localization In eukaryotic cells, proteins can be targeted to specific locales: ER, Golgi, Nucleus, mitochondrion or secreted Specific signal sequences interact with other proteins at the target site KDEL: Endoplasmic reticulum KRKR: Nucleus Hydrophobic residues: Secretion (Golgi) ...
... 1. Regulation by Localization In eukaryotic cells, proteins can be targeted to specific locales: ER, Golgi, Nucleus, mitochondrion or secreted Specific signal sequences interact with other proteins at the target site KDEL: Endoplasmic reticulum KRKR: Nucleus Hydrophobic residues: Secretion (Golgi) ...
RELIATech GmbH
... Reconstitution: The lyophilized IgG is stable at 4°C. for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C. Avoid repeated freeze-thaw cycles. Applications EL ...
... Reconstitution: The lyophilized IgG is stable at 4°C. for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C. Avoid repeated freeze-thaw cycles. Applications EL ...
Document
... crystallography and NMR spectroscopy. These experimental techniques are time consuming and not possible if a sufficient quantity and quality of a proteins is not available. The built model provides a wealth of information of how the protein functions with information at residue property level. This ...
... crystallography and NMR spectroscopy. These experimental techniques are time consuming and not possible if a sufficient quantity and quality of a proteins is not available. The built model provides a wealth of information of how the protein functions with information at residue property level. This ...
ppt
... We want to work with pure LEC1 protein. How do we purify the LEC1 protein from all the other E. coli proteins? ...
... We want to work with pure LEC1 protein. How do we purify the LEC1 protein from all the other E. coli proteins? ...
Reading guide - Chemistry Courses: About
... 4. How do some cells use actin to move? Explain, chemically, how this is an energy utilizing process. 5. How are microtubules structurally similar to microfilaments, and how are they different? 6. Why are there drugs that target microtubules? How do colchicine and taxol work in opposite ways to prod ...
... 4. How do some cells use actin to move? Explain, chemically, how this is an energy utilizing process. 5. How are microtubules structurally similar to microfilaments, and how are they different? 6. Why are there drugs that target microtubules? How do colchicine and taxol work in opposite ways to prod ...
PK-Focused Changes
... At times during the lead optimization process, a lead may be found to have drug-like binding to the target but suboptimal pharmacokinetics. Problems in pharmacokinetics can be linked to any of the four aspects of ADME – absorption, distribution, metabolism, and excretion. Some functional group repla ...
... At times during the lead optimization process, a lead may be found to have drug-like binding to the target but suboptimal pharmacokinetics. Problems in pharmacokinetics can be linked to any of the four aspects of ADME – absorption, distribution, metabolism, and excretion. Some functional group repla ...
Accounting for all the factors
... 1–14%. The authors said that performance was comparable to research tools that quantify gene expression. Assay output was not affected by the quantity of DNA used to transfect cells with the MRTU library, the quality of mRNA acquired for analysis or the number of PCR cycles. Thus, Makarov said, the ...
... 1–14%. The authors said that performance was comparable to research tools that quantify gene expression. Assay output was not affected by the quantity of DNA used to transfect cells with the MRTU library, the quality of mRNA acquired for analysis or the number of PCR cycles. Thus, Makarov said, the ...
signal transduction the ligand-gated ion channels
... along their axons (0.1 mm to 3 m) • Action potentials are initiated at the initial segment of the axon and are conducted down the axon at rates of 1 – 100m / sec • Many axons are insulated by a myelin sheath to increase the speed of transmission (many are not insulated) ...
... along their axons (0.1 mm to 3 m) • Action potentials are initiated at the initial segment of the axon and are conducted down the axon at rates of 1 – 100m / sec • Many axons are insulated by a myelin sheath to increase the speed of transmission (many are not insulated) ...
Ig, Struction and Function
... • The immunoglobulins are a group of glycoproteins present in the serum and tissue fluids of all mammals. ...
... • The immunoglobulins are a group of glycoproteins present in the serum and tissue fluids of all mammals. ...
Prediction of protein disorder: basic concepts and practical hints
... Contains 3 receptor binding motifs: xLxxLLx (LIG_NRBOX) ...
... Contains 3 receptor binding motifs: xLxxLLx (LIG_NRBOX) ...
In Anfinsen`s experiment, RNAse was denatured with urea and β
... treatment with oxygen, the enzyme’s activity was restored. What conclusions can be made from this? a) Urea and β-mercaptoethanol are ineffective denaturants. b) All the necessary information for protein folding is in the primary structure of RNAse. c) RNAse is particularly resistant to denaturation. ...
... treatment with oxygen, the enzyme’s activity was restored. What conclusions can be made from this? a) Urea and β-mercaptoethanol are ineffective denaturants. b) All the necessary information for protein folding is in the primary structure of RNAse. c) RNAse is particularly resistant to denaturation. ...
Cellular and Molecular Biology (HTH SCI 1I06) Legacy Summary
... or the presence of antigens (ex. peanut molecules in allergic reactions). Once released, these signaling molecules bind to receptors in order to produce their respective effects. Receptor proteins can be found on cell membranes, in the cytoplasm or in the nucleus. When a signaling molecule, also kno ...
... or the presence of antigens (ex. peanut molecules in allergic reactions). Once released, these signaling molecules bind to receptors in order to produce their respective effects. Receptor proteins can be found on cell membranes, in the cytoplasm or in the nucleus. When a signaling molecule, also kno ...
The lower Vmax is consistent with the mutation resulting in a
... mutant αCP1. (If done in parallel this would also obviate potential differences due to differences in the preparation methods.) One would then measure the initial rate of S6 kinase-catalyzed phosphorylation of wild type αCP1 in order to determine Vmax and Km. One would then repeat the experiment at ...
... mutant αCP1. (If done in parallel this would also obviate potential differences due to differences in the preparation methods.) One would then measure the initial rate of S6 kinase-catalyzed phosphorylation of wild type αCP1 in order to determine Vmax and Km. One would then repeat the experiment at ...
Problem set answers
... There is one other model that is fits the available data that does not require additive binding: AUF1 can bind E2epf and aCP1 at different sites that result in a conformation change that makes the other component bind tighter. This model would follow the same scheme as above but not require interact ...
... There is one other model that is fits the available data that does not require additive binding: AUF1 can bind E2epf and aCP1 at different sites that result in a conformation change that makes the other component bind tighter. This model would follow the same scheme as above but not require interact ...
1 Irreversible Enzyme Inhibition Affinity labels for studying enzyme
... • DFP reacts with the active site serine (Ser-195) of chymotrypsin to form DFP-chymotrypsin • Such organophosphorous inhibitors are used as insecticides or for enzyme research • These inhibitors are toxic because they inhibit acetylcholinesterase (a serine protease that hydrolyzes the neurotransmitt ...
... • DFP reacts with the active site serine (Ser-195) of chymotrypsin to form DFP-chymotrypsin • Such organophosphorous inhibitors are used as insecticides or for enzyme research • These inhibitors are toxic because they inhibit acetylcholinesterase (a serine protease that hydrolyzes the neurotransmitt ...
Mentor: James A. MacKay Students: Amanda Williams, Holly Sofka
... Students: Amanda Williams, Holly Sofka Project Description: Ribonucleic acid (RNA) is believed to be an important molecule in the evolution of life and has functionally taken on many important biological roles. Given the many functions of RNA, molecular recognition of RNA represents an attractive go ...
... Students: Amanda Williams, Holly Sofka Project Description: Ribonucleic acid (RNA) is believed to be an important molecule in the evolution of life and has functionally taken on many important biological roles. Given the many functions of RNA, molecular recognition of RNA represents an attractive go ...
Control of Cell Adhesion
... In order to produce cellular responses, many other molecular steps are necessary. The sequences of these events is generally referred to as “signal transduction pathways” ...
... In order to produce cellular responses, many other molecular steps are necessary. The sequences of these events is generally referred to as “signal transduction pathways” ...
Steps of Thyroid Hormone Synthesis 1. Tyrosine residues of
... 3. Thyroid hormones remain stored as part of thyroglobulin for up to three months. 4. TSH (thyroid stimulating hormone) acting by cAMP causes phosphorylation (activation) of the enzymes needed for thyroid hormone synthesis. 5. Follicular cells take in thyroglobulin by endocytosis. 6. The endosome fu ...
... 3. Thyroid hormones remain stored as part of thyroglobulin for up to three months. 4. TSH (thyroid stimulating hormone) acting by cAMP causes phosphorylation (activation) of the enzymes needed for thyroid hormone synthesis. 5. Follicular cells take in thyroglobulin by endocytosis. 6. The endosome fu ...
Monoclonal Antibodies - Home - KSU Faculty Member websites
... Affinity: the strength of binding between a single binding site and a single ligand. Avidity: the strength of binding between a molecule and a complex ligand, e.g. if there are multiple binding sites then the avidity may be increased by increasing the number of binding sites or by increasing the aff ...
... Affinity: the strength of binding between a single binding site and a single ligand. Avidity: the strength of binding between a molecule and a complex ligand, e.g. if there are multiple binding sites then the avidity may be increased by increasing the number of binding sites or by increasing the aff ...
Lecture 3. Hormone action - receptors
... REGULATION OF RECEPTOR BINDING 1. Homologous receptor regulation: a hormone affects the characteristics of its own receptors 2. Heterologous receptor regulation: a hormone affects the characteristics of other hormones’ receptors ...
... REGULATION OF RECEPTOR BINDING 1. Homologous receptor regulation: a hormone affects the characteristics of its own receptors 2. Heterologous receptor regulation: a hormone affects the characteristics of other hormones’ receptors ...
CD spectroscopy
... HSQC. The spectrum shows cross peaks between 15N and 1H in the NH group of the peptide bond, which thus creates one cross peak for every amino acid in the protein. In this picture the assignment of the spectrum is displayed onto the spectrum, which means that they have already found out which peak b ...
... HSQC. The spectrum shows cross peaks between 15N and 1H in the NH group of the peptide bond, which thus creates one cross peak for every amino acid in the protein. In this picture the assignment of the spectrum is displayed onto the spectrum, which means that they have already found out which peak b ...
Document
... While we will focus on biosensors (and a few molecular motors), they are based on the same interactions that occur naturally in biological systems and hence provide insight into biological systems opportunity to develop innovative uses of biological materials opportunity to apply engineering tools ...
... While we will focus on biosensors (and a few molecular motors), they are based on the same interactions that occur naturally in biological systems and hence provide insight into biological systems opportunity to develop innovative uses of biological materials opportunity to apply engineering tools ...