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EXAM 3
EXAM 3

... 5. In Labrador retrievers, two of the loci controlling coat color (black, chocolate and yellow) are the E locus and the B locus. At the B locus, black (B) is dominant to chocolate (b). At the E locus, homozygous recessive alleles at the E locus (ee) results in yellow pups regardless of genotype at t ...
Meiosis - Lamont High
Meiosis - Lamont High

... • Structure formed when chromosomes come together is called a tetrad • A tetrad consists of 2 replicated chromosomes or 2 chromatids • Chromatids are chromosomes that are still attached to their sister strand via a centromere • When centromere eventually splits and chromatids are free of their sist ...
24.8 brief comms MH - Department of Entomology
24.8 brief comms MH - Department of Entomology

... are therefore sacrificed to aid the others. Because multicellularity in social amoebae is accomplished by aggregation of cells, fruiting bodies could consist of one or more clones. In the model organism Dictyostelium discoideum, genetically distinct clones can mix to form chimaeras, and one clone ma ...
A long-term demasculinization of X
A long-term demasculinization of X

... We first profiled the transcriptomes of whole male and female D. melanogaster adults using Affymetrix whole-genome tiling arrays. The arrays used 3,116,816 25-nt probe pairs to assay transcription of 109,088,560 bp of repeat-free euchromatic genome. We detected a total of 35,884,625 bp (;32.89%) in ...
Educational Items Section Immunoglobulin Genes Atlas of Genetics and Cytogenetics
Educational Items Section Immunoglobulin Genes Atlas of Genetics and Cytogenetics

... A B-cell first expresses the IgM at its surface and then, during its differentiation, may express another class of Ig (IgG, IgE or IgA) (this mechanism is known as an isotype switch): how does this switch occur? How can we explain that regardless of the immunoglobulin isotype produced, the same spec ...
Literature retrieval
Literature retrieval

... percentage because some of the most investigated genes (p53 for example) have a baseline occurrence of 1%. (vi) Common phrase gene names (i.e., those for which each term in the name is a common word, such as ‘novel protein’) with an exceptionally high number of hits (more than 100) were manually cur ...
Linkage and Mapping 2
Linkage and Mapping 2

...  In this example, we will consider fruit flies that differ in ...
Cell Reproduction Mitosis and Meiosis aka Cell Division
Cell Reproduction Mitosis and Meiosis aka Cell Division

... • G1 Phase – the cell doubles in size, enzymes and organelles, such as mitochondria and ribosomes, roughly double in number • S Phase – the DNA that makes up the chromatin replicates – chromosomes double. Example: human chromosomes go from 46 chromosomes to 92 chromosomes • G2 Phase – the cell under ...
Gene Set Enrichment Analysis
Gene Set Enrichment Analysis

RNA AND PROTEIN SYNTHESIS
RNA AND PROTEIN SYNTHESIS

... RNA polymerase - the enzyme responsible for RNA transcription. Moves along gene and bonds appropriate RNA nucleotide to complimentary DNA nucleotide. Promoter - binding site on gene that RNA polymerase attaches to at the start of transcription. Codon - set of three mRNA nucleotides that code for an ...
Mouse_lecture
Mouse_lecture

... ~5% of coding genes in genome Highly conserved across vertebrates Flies and worms have unrelated GPCR families of odorant receptors. Identified later from mining the sequenced genomes in mid-90s. Worm GPCRs identified by Emily Troemel in Bargmann lab at UCSF. ...
Evolution
Evolution

... Mutations is CMT3 and KYP deregulate paternal transcription H3K9me2 is closely linked with DNA methylation by CHROMOMETHYLASE 3 (CMT3). CMT3 and KRYPTONITE (KYP), the enzyme responsible for much of H3K9me2 in Arabidopsis, but are related to silence transposable or foreign DNAs. It was found that a ...
Genetics introduction
Genetics introduction

... •Genotype of each parent? •What is the genotypic ratio? •What is the phenotypic ratio? •What percentage of the F1 generation will have a heterozygous genotype? •What is the probability that the 5th puppy will have a pink nose? ...
Cytoplasmic RNA improves accuracy of mRNA
Cytoplasmic RNA improves accuracy of mRNA

... 1 and 2 show results with modifications and samples 3 and 4 without modifications. C) qrtPCR showing higher retrieval of mature transcripts when modifications are added to the original Norgen protocol. The relative fold difference between exon expression and intron expression was measured in cytopla ...
C2005/F2401 Lect #22 - Columbia University
C2005/F2401 Lect #22 - Columbia University

... 3. Results: In this case (AABB X aabb), first parent must produce AB gametes and second parent ab gametes, so all zygotes must be AaBb = F1. (If you think gametes of first parent could be AA or BB, you should convince yourself why you are wrong. Put the genes on homologous chromosomes, go through me ...
Leukaemia Section Leukemia Variant (HCL-V) Atlas of Genetics and Cytogenetics
Leukaemia Section Leukemia Variant (HCL-V) Atlas of Genetics and Cytogenetics

... leukaemia. Br J Haematol. 1995 Dec;91(4):1025-30 ...
Slide 1
Slide 1

... mean of ill persons, such that some individuals may die of other causes before they even manifest symptoms of Huntington disease, this is termed "reduced/incomplete penetrance” ...
Should this dog be called Spot? Imagine this microscope drama. A
Should this dog be called Spot? Imagine this microscope drama. A

... 11. Does the female dog have curly hair? __________ 12. Does the male dog have curly hair? __________ 13. Define the term heterozygous ________________________________________________________ 14. For which traits is the puppy heterozygous? ____________________________________________ 15, Define the ...
Specialized techniques for site-directed mutagenesis in cyanobacteria
Specialized techniques for site-directed mutagenesis in cyanobacteria

... reading frame can exert polar effects on downstream genes. Additionally, when several genes within the same strain are to be inactivated, the use of many different antibiotics to select for various insertions can have cumulative, detrimental effects on cell culture viability. In light of these compl ...
Plant sex determination and sex chromosomes
Plant sex determination and sex chromosomes

... chromosome morphology (Wilby and Parker, 1986), are consistent with such a possibility, but it has not yet been tested. Some X-linked mutations are not masked by the Rumex Y chromosome (Smith, 1963), ie males are hemizygous for this region, like classical sex-linked loci in many animals. In Silence ...
Meiosis II
Meiosis II

... chromosomes will pair together and crossing over can occur. The homologous pair moves to the middle of the cell during metaphase I and separate during anaphase I, creating two haploid daughter cells in telophase I. The chromosomes condense in prophase II, and this time individual chromosomes move to ...
PDF
PDF

... progression were inferred. In order to identify these genes and investigate their function in later stages of meiosis, we created three models with diminishing Yp and Zfy gene complements (but lacking the Y-long-arm). Since the Y-long-arm mediates pairing and exchange with the X via their pseudoauto ...
Mendelian Genetics
Mendelian Genetics

... Genetic cross that considers only one characteristic. ...
File
File

... D) will be in 1/4 of her children on average * E) will be in 1/2 of her children on average 11. The total number of protein-coding genes in the human genome is most likely between A) 5-15,000. B) 15-35,000. * C) 35-75,000. D) 75-100,000. E) 100-150,000. 12. In an irradiation and gene transfer experi ...
The white gene
The white gene

... Suppose we isolate 5 curly wing mutations c1 c2 c3 c4 c5 We want to know how many genes are disrupted by these mutations and which mutations are in the same complementation group ...
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X-inactivation



X-inactivation (also called lyonization) is a process by which one of the two copies of the X chromosome present in female mammals is inactivated. The inactive X chromosome is silenced by its being packaged in such a way that it has a transcriptionally inactive structure called heterochromatin. As nearly all female mammals have two X chromosomes, X-inactivation prevents them from having twice as many X chromosome gene products as males, who only possess a single copy of the X chromosome (see dosage compensation). The choice of which X chromosome will be inactivated is random in placental mammals such as humans, but once an X chromosome is inactivated it will remain inactive throughout the lifetime of the cell and its descendants in the organism. Unlike the random X-inactivation in placental mammals, inactivation in marsupials applies exclusively to the paternally derived X chromosome.
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