Laser tweezers and their applications
... sample has been identified, it must be manipulated and possibly extracted in order for analysis to be possible, a function that miniature laser-tweezers would be perfectly suited to provide[23]. In order to manipulate very small objects, the laser tweezers must overcome diffraction limits. This has ...
... sample has been identified, it must be manipulated and possibly extracted in order for analysis to be possible, a function that miniature laser-tweezers would be perfectly suited to provide[23]. In order to manipulate very small objects, the laser tweezers must overcome diffraction limits. This has ...
CavityRingDown_Acous..
... the aperture smaller so the beams don’t overlap, measure the rise time and peak diffraction efficiency (not diffracted power, but ratio of diffracted output to input) as a function focal spot size, varied inversely ¢ = ¸F=A by opening and closing the aperture A (or perhaps by z-shifting the AOD). No ...
... the aperture smaller so the beams don’t overlap, measure the rise time and peak diffraction efficiency (not diffracted power, but ratio of diffracted output to input) as a function focal spot size, varied inversely ¢ = ¸F=A by opening and closing the aperture A (or perhaps by z-shifting the AOD). No ...
Aalborg Universitet Transfer function and near-field detection of evanescent waves
... geometry of the refractive index profile is depicted in Fig. 5. The core and cladding indices of the fiber are 1.459 and 1.457, respectively; the core diameter is 4 m, and, to increase computation speed, a reduced cladding diameter of 20 m was chosen. The opening angles were varied from 10° to 70° ...
... geometry of the refractive index profile is depicted in Fig. 5. The core and cladding indices of the fiber are 1.459 and 1.457, respectively; the core diameter is 4 m, and, to increase computation speed, a reduced cladding diameter of 20 m was chosen. The opening angles were varied from 10° to 70° ...
Microsoft Office PowerPoint 2003 Beta - poster#2
... (ca 7 nm for a 20-mer), and a fluorochrome molecule. The physical dimensions of the fluorochrome we use, Alexa 532, have not been determined but are likely to be on the order of several nm in diameter (Molecular Probes, Inc.). The practical upper limit to probe length is determined by the penetratio ...
... (ca 7 nm for a 20-mer), and a fluorochrome molecule. The physical dimensions of the fluorochrome we use, Alexa 532, have not been determined but are likely to be on the order of several nm in diameter (Molecular Probes, Inc.). The practical upper limit to probe length is determined by the penetratio ...
Lecture 31 - Purdue Physics
... determines how much light will reach the image plane. • Pupils are typically circular: the area varies as the square of the diameter, 9. • The image area varies as the square of the lateral dimension, : ~ • Light intensity at the image plane varies as 9/ • (9/ ) is called the relative aperture ...
... determines how much light will reach the image plane. • Pupils are typically circular: the area varies as the square of the diameter, 9. • The image area varies as the square of the lateral dimension, : ~ • Light intensity at the image plane varies as 9/ • (9/ ) is called the relative aperture ...
Optical forces and torques in non-uniform beams of
... pressure on a particle is most appropriate in the Rayleigh limit, when the particle’s size is no greater than the wavelength of light. In this limit, the three terms in g(r) may be interpreted as distinct mechanisms by which a beam of light exerts forces on illuminated objects. The first two terms i ...
... pressure on a particle is most appropriate in the Rayleigh limit, when the particle’s size is no greater than the wavelength of light. In this limit, the three terms in g(r) may be interpreted as distinct mechanisms by which a beam of light exerts forces on illuminated objects. The first two terms i ...
Optical forces and torques in non
... pressure on a particle is most appropriate in the Rayleigh limit, when the particle’s size is no greater than the wavelength of light. In this limit, the three terms in g(r) may be interpreted as distinct mechanisms by which a beam of light exerts forces on illuminated objects. The first two terms i ...
... pressure on a particle is most appropriate in the Rayleigh limit, when the particle’s size is no greater than the wavelength of light. In this limit, the three terms in g(r) may be interpreted as distinct mechanisms by which a beam of light exerts forces on illuminated objects. The first two terms i ...
Initial demonstration of a local, evanescent, array coupled biosensor concept
... In proof-of-concept experiments, the evanescent field has been monitored via an alternative method of near-field scanning optical microscopy (NSOM). Similar to the underlying detector array, NSOM is used to directly measure the evanescent field and thus the optical intensity on the upper surface of ...
... In proof-of-concept experiments, the evanescent field has been monitored via an alternative method of near-field scanning optical microscopy (NSOM). Similar to the underlying detector array, NSOM is used to directly measure the evanescent field and thus the optical intensity on the upper surface of ...
3D Visualization of Thylakoid Membrane
... in 2D and 3D (this wonderful video should not be missed!). Every stage of thylakoid development is shown in a traditional TEM view, outlining the membranes to be visualized in 3D. Then, the 3D structures of the outlined areas are presented and rotated to show membrane surfaces from many angles durin ...
... in 2D and 3D (this wonderful video should not be missed!). Every stage of thylakoid development is shown in a traditional TEM view, outlining the membranes to be visualized in 3D. Then, the 3D structures of the outlined areas are presented and rotated to show membrane surfaces from many angles durin ...
Far-field optical microscope with nanometer-scale
... droplets has been justified in the previous section, even more crucial question for the consideration of the far-field surface plasmon microscope performance is the surface plasmon propagation length. The importance of this question may again be illustrated in the case of infinitely thick metal film ...
... droplets has been justified in the previous section, even more crucial question for the consideration of the far-field surface plasmon microscope performance is the surface plasmon propagation length. The importance of this question may again be illustrated in the case of infinitely thick metal film ...
Electromagnetic forces for an arbitrary optical trapping of a spherical
... missing mechanical measurements in the world of microorganisms and cells that could be correlated to biochemical information. The importance of understanding the optical forces in dielectric beads under different incident beam conditions comes from the fact that they are the natural transducer for f ...
... missing mechanical measurements in the world of microorganisms and cells that could be correlated to biochemical information. The importance of understanding the optical forces in dielectric beads under different incident beam conditions comes from the fact that they are the natural transducer for f ...
Spatially resolved measurement of femtosecond
... surface with a translation speed of 1 mm∕ s. The dimensions of the refractive index changed region, shown in Fig. 3, were measured with an optical transmission microscope. The cross section in Fig. 3(b) shows a total depth d of 40 μm and a maximum width of 10 μm. Additionally, smaller subfeatures wi ...
... surface with a translation speed of 1 mm∕ s. The dimensions of the refractive index changed region, shown in Fig. 3, were measured with an optical transmission microscope. The cross section in Fig. 3(b) shows a total depth d of 40 μm and a maximum width of 10 μm. Additionally, smaller subfeatures wi ...
Optically induced forces in scanning probe microscopy
... of the light, with components (kx, ky, kz). It is assumed here that kx, ky are parallel to the surface of the sample, while kz is perpendicular to it. Thus the action of propagating information about an object to a detector in the far-field causes the attainable resolution to be limited to the order ...
... of the light, with components (kx, ky, kz). It is assumed here that kx, ky are parallel to the surface of the sample, while kz is perpendicular to it. Thus the action of propagating information about an object to a detector in the far-field causes the attainable resolution to be limited to the order ...
Example
... of diameter around 2 mm. Diffraction therefore limits the ability to resolve distance objects. Applying Rayleigh’s criterion, two “dots” (as shown) cannot be resolved if (=D/L) is less than R = 1.22 /d, where d is the diameter of the pupil. Note: the index of refraction inside the eye is simila ...
... of diameter around 2 mm. Diffraction therefore limits the ability to resolve distance objects. Applying Rayleigh’s criterion, two “dots” (as shown) cannot be resolved if (=D/L) is less than R = 1.22 /d, where d is the diameter of the pupil. Note: the index of refraction inside the eye is simila ...
Ay 105 Lab Experiment #P: Polarization!!!!
... Set up a rail with a laser at one end. Make sure you are able to rotate the laser on its mount. Add the cheap polarizer on a square mount in front of the laser. On the other end1 mount the lens and photodiode and focus the laser onto the photodiode. Rotate the laser so that it’s in line with the pol ...
... Set up a rail with a laser at one end. Make sure you are able to rotate the laser on its mount. Add the cheap polarizer on a square mount in front of the laser. On the other end1 mount the lens and photodiode and focus the laser onto the photodiode. Rotate the laser so that it’s in line with the pol ...
[pdf]
... tomography [25-28] extended to the near-field [29-43]. This scheme is particularly well suited for planar source-detection geometries that arise for example, in breast imaging. The methodology has attracted attention [29-43] because the diffusive wave modes derived using the angular-spectrum represe ...
... tomography [25-28] extended to the near-field [29-43]. This scheme is particularly well suited for planar source-detection geometries that arise for example, in breast imaging. The methodology has attracted attention [29-43] because the diffusive wave modes derived using the angular-spectrum represe ...
Image formation and optical transfer function in a course of
... approach it is possible to give the Point Spread Function as a distribution of dots that consists of the cuts of the rays with the image plane. In many books1'5 the point spread function and the image of extended objects is treated. They generaly deal with incoherent or coherent illumination. ...
... approach it is possible to give the Point Spread Function as a distribution of dots that consists of the cuts of the rays with the image plane. In many books1'5 the point spread function and the image of extended objects is treated. They generaly deal with incoherent or coherent illumination. ...
Laser Molecular Spectroscopy CHE466 Fall 2007
... For a pulsed laser, E is the energy output measured using a Joule meter and t is the time duration of the pulse. In a CW laser, the power is measured directly with a power meter. The power flux is the power per unit area: F = P/A ...
... For a pulsed laser, E is the energy output measured using a Joule meter and t is the time duration of the pulse. In a CW laser, the power is measured directly with a power meter. The power flux is the power per unit area: F = P/A ...
2 Modeling and Design of Lens Systems
... Note: If D=0 an other arrangement of the above sequence of four operations is useful propagation to the exact focus point. 3) Transformation of a spherical wave: Consider the illumination of the system with a spherical wave with radius of curvature R1 ...
... Note: If D=0 an other arrangement of the above sequence of four operations is useful propagation to the exact focus point. 3) Transformation of a spherical wave: Consider the illumination of the system with a spherical wave with radius of curvature R1 ...
Measuring amplitude and phase of light emerging from
... simplified formula derived as 'z = ·n / (NA2) with n the refractive index of the object space9. The calculated axial resolution at 642 nm wavelength in air is 'z = 793 nm. At 100X magnification, a pixel on a charge-coupled device (CCD) sensor (Sicon Corporation, CFW1312M camera with SONY ICX205AK i ...
... simplified formula derived as 'z = ·n / (NA2) with n the refractive index of the object space9. The calculated axial resolution at 642 nm wavelength in air is 'z = 793 nm. At 100X magnification, a pixel on a charge-coupled device (CCD) sensor (Sicon Corporation, CFW1312M camera with SONY ICX205AK i ...
Template for Electronic Submission to ACS Journals - Lirias
... Under the experimental conditions described in the Methods section, we could convert ~50% of the protein sample to the red state, at which point the rate of photodestruction of the red state due to the 405-nm light became dominant over the photoconversion (Supporting Figure 4). The graphs in Support ...
... Under the experimental conditions described in the Methods section, we could convert ~50% of the protein sample to the red state, at which point the rate of photodestruction of the red state due to the 405-nm light became dominant over the photoconversion (Supporting Figure 4). The graphs in Support ...
Intraocular Lenses
... e the aperture into circular “zones” (these are the concentric rings in the zone plate pictured above). The edge of each zone is /2 farther away from Po than the edge of the neighboring zone. 2. Notice that the wave that travels along the axis of the aperture experiences constructive interference ...
... e the aperture into circular “zones” (these are the concentric rings in the zone plate pictured above). The edge of each zone is /2 farther away from Po than the edge of the neighboring zone. 2. Notice that the wave that travels along the axis of the aperture experiences constructive interference ...
On the diffraction limit for lensless imaging
... on the wave theory of light [6]. The best known quantity shown in Table 1 is the radius AvMry of the central maximum (Airy disk). Because 84 % of the total flux is concentrated in the central disk, it is often assumed that the diffraction pattern consists of the Airy disk alone. Figure 3 shows at a ...
... on the wave theory of light [6]. The best known quantity shown in Table 1 is the radius AvMry of the central maximum (Airy disk). Because 84 % of the total flux is concentrated in the central disk, it is often assumed that the diffraction pattern consists of the Airy disk alone. Figure 3 shows at a ...
r 5 fs hinner ljuset färdas 1
... modes. Hint: If you click inside a window, you can read the time (frequency) and intensity for this time (frequency). Set a short cavity length of about 0.1 m. How is the pulse shape influenced by the number of modes? (For a given cavity length this is coupled to the spectral width). How does the nu ...
... modes. Hint: If you click inside a window, you can read the time (frequency) and intensity for this time (frequency). Set a short cavity length of about 0.1 m. How is the pulse shape influenced by the number of modes? (For a given cavity length this is coupled to the spectral width). How does the nu ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.