Novel 3-D microscopy techniques - Purdue University Cytometry
... Effect of increased incident power on generation of signal. Samples of acidfucsin-stained monkey kidney were imaged at a depth of 60 µm into the sample by confocal (550 µW of 532-nm light) and by multiphoton (12 mW of 1047-nm light) microscopy. Laser intensities were adjusted to produce the same mea ...
... Effect of increased incident power on generation of signal. Samples of acidfucsin-stained monkey kidney were imaged at a depth of 60 µm into the sample by confocal (550 µW of 532-nm light) and by multiphoton (12 mW of 1047-nm light) microscopy. Laser intensities were adjusted to produce the same mea ...
Optical Negative Refraction in Ferrofluids with Magnetocontrollability Y. Gao, J. P. Huang,
... ferrofluid system at an angle ¼ 30 with respect to z axis. Here just denotes the angle of incidence. Using Eq. (1), we obtain "xx ¼ 4:43 þ 0:15i and "zz ¼ 3:29 þ 0:19i according to the parameters: "1 ¼ 21:4 þ 0:9i, "2 ¼ 1:77, p ¼ 0:22, n ¼ 50, and the length of each chain is 1 m. Here "1 has ...
... ferrofluid system at an angle ¼ 30 with respect to z axis. Here just denotes the angle of incidence. Using Eq. (1), we obtain "xx ¼ 4:43 þ 0:15i and "zz ¼ 3:29 þ 0:19i according to the parameters: "1 ¼ 21:4 þ 0:9i, "2 ¼ 1:77, p ¼ 0:22, n ¼ 50, and the length of each chain is 1 m. Here "1 has ...
CP1: Investigation into the Feasibility of a Three Axis
... issue often ignored in other experiments: the changing of the optical properties of the cell itself during a process of interest (in this case the action potential). Another issue in biological settings involves the restrictions that the shape and size of the sample (and its necessary environment) p ...
... issue often ignored in other experiments: the changing of the optical properties of the cell itself during a process of interest (in this case the action potential). Another issue in biological settings involves the restrictions that the shape and size of the sample (and its necessary environment) p ...
3D widefield light microscopy with better than 100 nm axial resolution
... independent and can with advantage be used at the same time (I5M). In either mode, acquisition proceeds just as in normal computational deconvolution microscopy (Agard et al., 1989): a focal series of images are recorded, with the ...
... independent and can with advantage be used at the same time (I5M). In either mode, acquisition proceeds just as in normal computational deconvolution microscopy (Agard et al., 1989): a focal series of images are recorded, with the ...
Light Field Microscopy - Stanford Computer Graphics Laboratory
... Figure 3: The geometry of microscope light fields. (a) In an unrestricted optical system, cones of rays from points A on the object are refracted by lens B, reconverging to points on the intermediate image plane (off-page) at C. In an ideal lens, the centroids of these cones (red lines) converge at ...
... Figure 3: The geometry of microscope light fields. (a) In an unrestricted optical system, cones of rays from points A on the object are refracted by lens B, reconverging to points on the intermediate image plane (off-page) at C. In an ideal lens, the centroids of these cones (red lines) converge at ...
Analysing the potential for application of the phase shift method in
... diagram shown in Fig. 2. Therefore based on the records and analyses of the deformation pattern of particular diffraction fringes we can conclude about qualitative and quantitative surface deformations of the examined object. Since the observed dimensions of the surface defects are not only the func ...
... diagram shown in Fig. 2. Therefore based on the records and analyses of the deformation pattern of particular diffraction fringes we can conclude about qualitative and quantitative surface deformations of the examined object. Since the observed dimensions of the surface defects are not only the func ...
Notes on Waves - Anderson High School
... • Constructive Interference – when two (or more) waves meet to make a bigger wave • Destructive Interference – when two (or more) waves meet to make a smaller wave ...
... • Constructive Interference – when two (or more) waves meet to make a bigger wave • Destructive Interference – when two (or more) waves meet to make a smaller wave ...
Controllable Optical Phase Shift Over One Radian from a Single
... Small phase shifts have recently been observed from single atoms [5] and molecules [6]. Similar small phase shifts, arising from the shift of atomic energy levels, have also been observed by interferometric measurements of fluorescence from single trapped ions [7]. The interferometric techniques use ...
... Small phase shifts have recently been observed from single atoms [5] and molecules [6]. Similar small phase shifts, arising from the shift of atomic energy levels, have also been observed by interferometric measurements of fluorescence from single trapped ions [7]. The interferometric techniques use ...
Gullstrand equation
... equation is obtained by means of force balance,variational method, thermodynamic arguments, etc, starting from basic cases to the general equation [13, 16, 17]. Finally, the Gullstrand equation is valid in paraxial6 or first-order optics. This approximation considers only monochromatic light rays th ...
... equation is obtained by means of force balance,variational method, thermodynamic arguments, etc, starting from basic cases to the general equation [13, 16, 17]. Finally, the Gullstrand equation is valid in paraxial6 or first-order optics. This approximation considers only monochromatic light rays th ...
image
... A virtual image is formed when light rays do not pass through the image point but only appear to diverge from that point ...
... A virtual image is formed when light rays do not pass through the image point but only appear to diverge from that point ...
File
... Nearer the lens and the cone of rays refracted through the sagittal ( horizontal) plane BRS comes to focus at the P2 away from the lens. All rays pass through a horizontal line passing through P1 called the primary image and also through a vertical line passing through P2 called the secondary image. ...
... Nearer the lens and the cone of rays refracted through the sagittal ( horizontal) plane BRS comes to focus at the P2 away from the lens. All rays pass through a horizontal line passing through P1 called the primary image and also through a vertical line passing through P2 called the secondary image. ...
Coherence properties of light propagated through a
... The spatial coherence properties of wavefields are of fundamental importance since they play a key role in many optical imaging techniques.1 For example, it is well-known that partially coherent wavefields have the potential to carry more information content than fully coherent wavefields and that t ...
... The spatial coherence properties of wavefields are of fundamental importance since they play a key role in many optical imaging techniques.1 For example, it is well-known that partially coherent wavefields have the potential to carry more information content than fully coherent wavefields and that t ...
Ultra–large field-of-view two-photon microscopy
... of concerted study [3-5] and may be best examined in mice, where neurovascular function can be manipulated through both behavior and genetics [6, 7]. The brain-wide nature of these dynamics [8] implies the need for a microscope that can resolve micrometer-scale changes in vessel diameter across all ...
... of concerted study [3-5] and may be best examined in mice, where neurovascular function can be manipulated through both behavior and genetics [6, 7]. The brain-wide nature of these dynamics [8] implies the need for a microscope that can resolve micrometer-scale changes in vessel diameter across all ...
Document
... surfaces 22.2.1 Refraction at a plane surface • Refraction is defined as the changing of direction of a light ray and its speed of propagation as it passes from one medium into another. • Laws of refraction state : – The incident ray, the refracted ray and the normal all lie in the same plane. – For ...
... surfaces 22.2.1 Refraction at a plane surface • Refraction is defined as the changing of direction of a light ray and its speed of propagation as it passes from one medium into another. • Laws of refraction state : – The incident ray, the refracted ray and the normal all lie in the same plane. – For ...
Light Microscopy [10 credits]
... Confocal microscopy: Confocality & confocal microscopy methods Confocal microscopy – Components 1 Confocal microscopy – Components 2 Image acquisition parameters Samples for light microscopy: Sample preparation for light microscopy Sample labelling Live specimen imaging Optimisation of image acquisi ...
... Confocal microscopy: Confocality & confocal microscopy methods Confocal microscopy – Components 1 Confocal microscopy – Components 2 Image acquisition parameters Samples for light microscopy: Sample preparation for light microscopy Sample labelling Live specimen imaging Optimisation of image acquisi ...
Technical Information on Optics
... and refracted unpolarized light striking a non-absorbing optical medium having a refractive index of n' at an angle of incidence α. In the process, the reflected ray at the angle of reflection becomes partially polarized. The Fresnel equation gives the intensities of these beams according to their p ...
... and refracted unpolarized light striking a non-absorbing optical medium having a refractive index of n' at an angle of incidence α. In the process, the reflected ray at the angle of reflection becomes partially polarized. The Fresnel equation gives the intensities of these beams according to their p ...
Digital holography of total internal reflection
... imaging of phase profile of light in total internal reflection [25]. The technique, termed total internal reflection holographic microscopy (TIRHM), is based on the realization that the presence of materials on the interface modulates the phase profile of light in total internal reflection. TIRHM im ...
... imaging of phase profile of light in total internal reflection [25]. The technique, termed total internal reflection holographic microscopy (TIRHM), is based on the realization that the presence of materials on the interface modulates the phase profile of light in total internal reflection. TIRHM im ...
12. CONFOCAL MICROSCOPY • Confocal microscopy can render
... confocal microscope. Aberration correction, adaptive optics, is a critical issue especially when operating at very high NA (current research) However, even with an instrument that is capable in principle of producing high resolution images, the amount of information in the final image depends also ...
... confocal microscope. Aberration correction, adaptive optics, is a critical issue especially when operating at very high NA (current research) However, even with an instrument that is capable in principle of producing high resolution images, the amount of information in the final image depends also ...
Ray Optics - Sakshi Education
... 1. Light is a form of energy which on striking the eye makes things visible (stimulates the sensation of vision). 2. A substance through which light can more or less pass is called optical medium. 3. A medium through which light can pass very easily is called transparent medium. 4. A medium through ...
... 1. Light is a form of energy which on striking the eye makes things visible (stimulates the sensation of vision). 2. A substance through which light can more or less pass is called optical medium. 3. A medium through which light can pass very easily is called transparent medium. 4. A medium through ...
Simulating and optimizing compound refractive lens-based X
... this approximation is no longer valid). We present a formalism for CRL-based imaging systems utilizing an RTM approach to model archetypal X-ray imaging systems in a lens-by-lens manner, thus accounting for both thin- and thick-lens conditions. Attenuation by the lens material is calculated using RT ...
... this approximation is no longer valid). We present a formalism for CRL-based imaging systems utilizing an RTM approach to model archetypal X-ray imaging systems in a lens-by-lens manner, thus accounting for both thin- and thick-lens conditions. Attenuation by the lens material is calculated using RT ...
Photoacoustic microscopy with 2
... In the first-generation optical-resolution PAM,8 light was delivered in free space; thus, the object had to be moved in order to form an image. In contrast, in our 2-m PAM, a singlemode optical fiber 共P1-460A-FC-2, Thorlabs兲 guides light to the imaging probe, which not only enables scanning the pro ...
... In the first-generation optical-resolution PAM,8 light was delivered in free space; thus, the object had to be moved in order to form an image. In contrast, in our 2-m PAM, a singlemode optical fiber 共P1-460A-FC-2, Thorlabs兲 guides light to the imaging probe, which not only enables scanning the pro ...
Optical System design
... made by changing many design parameters simultaneously. This requires a true non-linear optimisation algorithm. The software uses damped least squares as the basic optimisation method, but with several enhancements [4], such as an extended line search option. The merit function is powerful and flexi ...
... made by changing many design parameters simultaneously. This requires a true non-linear optimisation algorithm. The software uses damped least squares as the basic optimisation method, but with several enhancements [4], such as an extended line search option. The merit function is powerful and flexi ...
Astronomy 101 Lab: Telescopes
... disk and place it between the lens and the screen. Rotate the wheel so that you have the largest possible opening. Make sure that you hold the disk close to the lens, such that you still see a clear image on the screen. Now, slowly turn the wheel to allow less and less light to pass through to the s ...
... disk and place it between the lens and the screen. Rotate the wheel so that you have the largest possible opening. Make sure that you hold the disk close to the lens, such that you still see a clear image on the screen. Now, slowly turn the wheel to allow less and less light to pass through to the s ...
All-angle negative refraction and imaging in a visible region
... dielectric matrix. When the separation between the nanowires is much smaller than the incident wavelength, these structures can be characterized as indefinite media, whose effective permittivities perpendicular and parallel to the wires are opposite in signs. Under this condition, the dispersion dia ...
... dielectric matrix. When the separation between the nanowires is much smaller than the incident wavelength, these structures can be characterized as indefinite media, whose effective permittivities perpendicular and parallel to the wires are opposite in signs. Under this condition, the dispersion dia ...
Superlens
A practical superlens, or super lens, is a lens which uses metamaterials to go beyond the diffraction limit. The diffraction limit is a feature of conventional lenses and microscopes that limits the fineness of their resolution. Many lens designs have been proposed that go beyond the diffraction limit in some way, but there are constraints and obstacles involved in realizing each of them.