Theory of confocal fluorescence imaging in the
... be tilted at an angle of 6 108 to the normal (Montamedi et al., 1997). The use of a DMD for laser-based confocal imaging in reflection mode was reported recently (Liang et al., 1997). Figure 1 presents a schematic for a fluorescence programmable array microscope system based on the DMD. The latter i ...
... be tilted at an angle of 6 108 to the normal (Montamedi et al., 1997). The use of a DMD for laser-based confocal imaging in reflection mode was reported recently (Liang et al., 1997). Figure 1 presents a schematic for a fluorescence programmable array microscope system based on the DMD. The latter i ...
what is light? - Fort Thomas Independent Schools
... They do this by a series of total internal reflections. Optical fibers are useful for getting light to inaccessible places. Mechanics and machinists use them to look at the interiors of engines, and physicians use them to look inside a patient’s body. ...
... They do this by a series of total internal reflections. Optical fibers are useful for getting light to inaccessible places. Mechanics and machinists use them to look at the interiors of engines, and physicians use them to look inside a patient’s body. ...
Precision High Numerical Aperture Scanning System for
... speed and position. The focal spot volume is on the order of 1 μm, thus high position accuracy is needed to ensure uniformity of the overlap and minimal scattering of the refractive structure. The scanning system must also be able to cover up to an 8 mm range to correct over the entire pupil of the ...
... speed and position. The focal spot volume is on the order of 1 μm, thus high position accuracy is needed to ensure uniformity of the overlap and minimal scattering of the refractive structure. The scanning system must also be able to cover up to an 8 mm range to correct over the entire pupil of the ...
chapter35
... with larger index of refraction to a medium with smaller n, as in the diagram n1 > n2. The refracted angle is larger than the incident angle. As the incident angle increases to the point that the refracted angle is 90o, this incident angle is called the critical angle θc. Beyond it, there is no refr ...
... with larger index of refraction to a medium with smaller n, as in the diagram n1 > n2. The refracted angle is larger than the incident angle. As the incident angle increases to the point that the refracted angle is 90o, this incident angle is called the critical angle θc. Beyond it, there is no refr ...
The Photoelectric Effect
... Monochromatic light (in this case produced by a broadband mercury lamp passing through a wavelengthselective interference filter) is incident on the cathode K of the photocell. This is typically made from potassium. This causes electrons to be liberated from the cathode, which then flow to ground vi ...
... Monochromatic light (in this case produced by a broadband mercury lamp passing through a wavelengthselective interference filter) is incident on the cathode K of the photocell. This is typically made from potassium. This causes electrons to be liberated from the cathode, which then flow to ground vi ...
AO for high peak power lasers
... 2. Controlled environment (stable temperature, indoors, enclosed beams) 3. No need for an extended field of view (point focus only) 4. Only slow response needed (at present . . .) Things that make AO for high-power lasers harder 1. Too much light (need to protect the wavefront sensor from the laser) ...
... 2. Controlled environment (stable temperature, indoors, enclosed beams) 3. No need for an extended field of view (point focus only) 4. Only slow response needed (at present . . .) Things that make AO for high-power lasers harder 1. Too much light (need to protect the wavefront sensor from the laser) ...
Ongoing Research Works
... compared with a single dye molecule. Moreover, as the dye is trapped inside the silica matrix, which provides an effective barrier keeping the dye from the surrounding environment, both photobleaching and photodegration phenomenon that often affect conventional dyes can be minimized. Therefore, exce ...
... compared with a single dye molecule. Moreover, as the dye is trapped inside the silica matrix, which provides an effective barrier keeping the dye from the surrounding environment, both photobleaching and photodegration phenomenon that often affect conventional dyes can be minimized. Therefore, exce ...
Machine Vision Systems as Shop Floor Metrology Tool
... not be able to provide the flexibility of scanning type systems for CAD data generation applications. APPLICATION TESTING As has already been stated, the key operational parameters needed for production machine vision include speed, resolution, and robustness especially to changing part surface cond ...
... not be able to provide the flexibility of scanning type systems for CAD data generation applications. APPLICATION TESTING As has already been stated, the key operational parameters needed for production machine vision include speed, resolution, and robustness especially to changing part surface cond ...
Light sheet-based fluorescence microscopy: more dimensions, more
... The same holds true in confocal fluorescence microscopy. Optical sectioning is not obtained in the illumination process, but by discriminating against the out of focus fluorescence light with a pinhole in front of the detector. Hence, most of the light emitted by the specimen does not reach the dete ...
... The same holds true in confocal fluorescence microscopy. Optical sectioning is not obtained in the illumination process, but by discriminating against the out of focus fluorescence light with a pinhole in front of the detector. Hence, most of the light emitted by the specimen does not reach the dete ...
Quadriwave lateral shearing interferometry
... Wavefront sensing is a well known technology for studying the aberrations of a light-beam. In most applications, only the lower-order aberrations (like spherical aberration or coma) are considered, because the lower the aberration order is, the stronger is the effect on the beam [12] [13]. As a cons ...
... Wavefront sensing is a well known technology for studying the aberrations of a light-beam. In most applications, only the lower-order aberrations (like spherical aberration or coma) are considered, because the lower the aberration order is, the stronger is the effect on the beam [12] [13]. As a cons ...
Measurement of spectacle lenses: A review
... experimental purposes and a few are the base for the commercial lensmeters available these days. ...
... experimental purposes and a few are the base for the commercial lensmeters available these days. ...
On the diffraction limit for lensless imaging
... where the notation is the same as in Eq. (8) but v and I a (M , V) P are replaced by their arithmetic means for each element of summation. Equation (10) was used to generate lists, accurate to six digits, of/(Av/V«) for consecutive upper limits A^ < 500 and increments 8v = 0.01M. These lists were us ...
... where the notation is the same as in Eq. (8) but v and I a (M , V) P are replaced by their arithmetic means for each element of summation. Equation (10) was used to generate lists, accurate to six digits, of/(Av/V«) for consecutive upper limits A^ < 500 and increments 8v = 0.01M. These lists were us ...
Applied physics viva
... A2. The distance between the optical centre of the lens and its focus is called focal length. Q3. On what factors does the focal length of a lens depend? A3. Focal length depends upon: Radii of curvature of the surfaces. (i)Refractive index of the medium of lens. (ii)Colour of rays and (iii)Media of ...
... A2. The distance between the optical centre of the lens and its focus is called focal length. Q3. On what factors does the focal length of a lens depend? A3. Focal length depends upon: Radii of curvature of the surfaces. (i)Refractive index of the medium of lens. (ii)Colour of rays and (iii)Media of ...
Lecture_22
... 32-6 Visible Spectrum and Dispersion Conceptual Example 32-10: Observed color of light under water. We said that color depends on wavelength. For example, for an object emitting 650 nm light in air, we see red. But this is true only in air. If we observe this same object when under water, it still ...
... 32-6 Visible Spectrum and Dispersion Conceptual Example 32-10: Observed color of light under water. We said that color depends on wavelength. For example, for an object emitting 650 nm light in air, we see red. But this is true only in air. If we observe this same object when under water, it still ...
may10-94 as a Word 6.0 doc - Lyle School of Engineering
... 14. Calculate the numerical aperture of a step index fiber with a core index of 1.47, a cladding index of 1.465, a core radius of 4 m, and a cladding radius of 100 m. The indices of refraction are specified at = 1.55 m. 15. Consider an LED with a quantum efficiency of 50%, emitting at a wavelen ...
... 14. Calculate the numerical aperture of a step index fiber with a core index of 1.47, a cladding index of 1.465, a core radius of 4 m, and a cladding radius of 100 m. The indices of refraction are specified at = 1.55 m. 15. Consider an LED with a quantum efficiency of 50%, emitting at a wavelen ...
High Harmonic Generation
... 1. When the intensity high enough, electrons can tunnel through the barrier into the continuum. This is called first step. 2. The laser field accelerates the electron away from the parent ion and drives it back when the electric field sign is changed. During this process the electron gains kinetic e ...
... 1. When the intensity high enough, electrons can tunnel through the barrier into the continuum. This is called first step. 2. The laser field accelerates the electron away from the parent ion and drives it back when the electric field sign is changed. During this process the electron gains kinetic e ...
Optical Monte Carlo modeling of a true port wine stain anatomy
... of clinical results which are not in agreement with existing laser-tissue interaction analyses. We have developed a unique optical Monte Carlo program which can simulate light propagation in any user-defined three-dimensional tissue geometry, and used this program to determine the volumetric energy ...
... of clinical results which are not in agreement with existing laser-tissue interaction analyses. We have developed a unique optical Monte Carlo program which can simulate light propagation in any user-defined three-dimensional tissue geometry, and used this program to determine the volumetric energy ...
Study the Effect of the Sugar Solutions on the Rotation of the
... power and angle of rotation is called “optical activity”. Two types of light source are used. The first one was the xenon lamp and chooses three color from it (red, green, and blue) using the proper filters, and the second source was He-Ne laser of wavelength 632nm. In each case, the rotation angle ...
... power and angle of rotation is called “optical activity”. Two types of light source are used. The first one was the xenon lamp and chooses three color from it (red, green, and blue) using the proper filters, and the second source was He-Ne laser of wavelength 632nm. In each case, the rotation angle ...
Shifting the spherical focus of a 4Pi focusing system
... aberration. Furthermore, for a Herschel-type focusing system the apodizer factor due to the conservation of energy appearing in the Richards–Wolf integral (1) and (2) is simply X(θ) = 1, for which a fundamental radial polarization mode is focused into a spherical spot centered at the focus. For an a ...
... aberration. Furthermore, for a Herschel-type focusing system the apodizer factor due to the conservation of energy appearing in the Richards–Wolf integral (1) and (2) is simply X(θ) = 1, for which a fundamental radial polarization mode is focused into a spherical spot centered at the focus. For an a ...
Extended depth-of-field iris recognition system for a
... field of view determines the spatial extent of the scene acquired by the sensor. Depth of field determines how far a planar object can move away from the best focus position and still be imaged without focus errors. The exposure period is defined as the detector integration time while acquiring an i ...
... field of view determines the spatial extent of the scene acquired by the sensor. Depth of field determines how far a planar object can move away from the best focus position and still be imaged without focus errors. The exposure period is defined as the detector integration time while acquiring an i ...
Review ! a
... ! The interferometer consists of a light source in the form of a laser emitting coherent light with wavelength ! = 632.8 nm ! The light passes through a de-focusing lens to spread out the normally very narrowly focused laser beam ! The light then passes through a half-silvered mirror m1 ...
... ! The interferometer consists of a light source in the form of a laser emitting coherent light with wavelength ! = 632.8 nm ! The light passes through a de-focusing lens to spread out the normally very narrowly focused laser beam ! The light then passes through a half-silvered mirror m1 ...
biology 163 laboratory use of the compound light microscope
... Use the following procedure to properly set up the microscope for viewing specimens. Refer to Appendix F if you are unfamiliar with the parts of the microscope! 1. Using the condenser height adjustment knob, place the condenser in its uppermost position. Open the condenser diaphragm fully by slidin ...
... Use the following procedure to properly set up the microscope for viewing specimens. Refer to Appendix F if you are unfamiliar with the parts of the microscope! 1. Using the condenser height adjustment knob, place the condenser in its uppermost position. Open the condenser diaphragm fully by slidin ...