Lipid modifications of proteins – slipping in and out of membranes
... employed by trans-membrane proteins. Lipid modifications facilitate the attachment of soluble proteins to biological membranes, but they also enable protein–protein interactions and, in some cases, the shuttling of proteins between the plasma membrane and the cytosol or other membrane compartments. ...
... employed by trans-membrane proteins. Lipid modifications facilitate the attachment of soluble proteins to biological membranes, but they also enable protein–protein interactions and, in some cases, the shuttling of proteins between the plasma membrane and the cytosol or other membrane compartments. ...
BIRKBECK COLLEGE
... Date: Thursday, 1st September 2011 Time: 3 hours You will be given a start time with your exam instructions Students will be required to answer 10 out of 15 questions. All questions carry 10 marks each. Each question must start on a new page and the question number written at the top of each sheet. ...
... Date: Thursday, 1st September 2011 Time: 3 hours You will be given a start time with your exam instructions Students will be required to answer 10 out of 15 questions. All questions carry 10 marks each. Each question must start on a new page and the question number written at the top of each sheet. ...
Signaling Through Scaffold, Anchoring, and Adaptor Proteins
... mechanism of ligand-binding to PTB domains, in which the peptide binds as an additional strand to an antiparallel b sheet (13). A distinguishing feature of PDZ domains is their recognition of short peptides with a COOH-terminal hydrophobic residue and a free carboxylate group, as exemplified by the ...
... mechanism of ligand-binding to PTB domains, in which the peptide binds as an additional strand to an antiparallel b sheet (13). A distinguishing feature of PDZ domains is their recognition of short peptides with a COOH-terminal hydrophobic residue and a free carboxylate group, as exemplified by the ...
Directed Proteomics Identifies a Plant
... mechanisms that recognize microbial invaders and subsequently activate defense responses. Although genetic approaches have shown that the various resistance genes activate multiple signal transduction pathways and that common defense responses can be activated via independent pathways (Innes, 1998), ...
... mechanisms that recognize microbial invaders and subsequently activate defense responses. Although genetic approaches have shown that the various resistance genes activate multiple signal transduction pathways and that common defense responses can be activated via independent pathways (Innes, 1998), ...
The P5 protein from bacteriophage phi
... a newly evolved molecular function and call for further experimental characterization of this unusual P5 protein. Keywords: structure prediction; bacteriophage phi-6; peptidase; lytic transglycosylase; lysozyme; comparative phage genomics ...
... a newly evolved molecular function and call for further experimental characterization of this unusual P5 protein. Keywords: structure prediction; bacteriophage phi-6; peptidase; lytic transglycosylase; lysozyme; comparative phage genomics ...
No Slide Title
... Inclusion cell disease (I cell disease) - rare disorder in which almost all hydrolytic enzymes are missing from lysosome I cell disease - single gene, recessive defect ...
... Inclusion cell disease (I cell disease) - rare disorder in which almost all hydrolytic enzymes are missing from lysosome I cell disease - single gene, recessive defect ...
Enzymes: Regulation 2-3
... consensus sequence in all target proteins phosphorylated by protein kinase A: Ser or Thr in this consensus sequence: ….–Arg–Arg–X–Ser–Z–… or …. –Arg–Arg–X–Thr–Z–… X = a small amino acid residue; Z = a large hydrophobic residue • Protein Kinase A binds other substrate protein sequences with a much lo ...
... consensus sequence in all target proteins phosphorylated by protein kinase A: Ser or Thr in this consensus sequence: ….–Arg–Arg–X–Ser–Z–… or …. –Arg–Arg–X–Thr–Z–… X = a small amino acid residue; Z = a large hydrophobic residue • Protein Kinase A binds other substrate protein sequences with a much lo ...
Properties of blood
... plasma. The normal value depends on the method of determination. It is 1.5 by electrophoresis and 1.2 by the chemical precipitation method. • The A/G is a non specific test that could help clinically in diagnosis of some diseases, e.g. it is decreased in liver diseases (due to decrease in albumin sy ...
... plasma. The normal value depends on the method of determination. It is 1.5 by electrophoresis and 1.2 by the chemical precipitation method. • The A/G is a non specific test that could help clinically in diagnosis of some diseases, e.g. it is decreased in liver diseases (due to decrease in albumin sy ...
Overview of Microarray Types
... DNA microarrays (also called Gene Chips) are devices not much larger than postage stamps. They are based printed on a glass substrate containing as many as 400,000 tiny cells each containing a microscopic spot of DNA. Each microscopic spot holds a short, synthetic, single-stranded DNA sequence from ...
... DNA microarrays (also called Gene Chips) are devices not much larger than postage stamps. They are based printed on a glass substrate containing as many as 400,000 tiny cells each containing a microscopic spot of DNA. Each microscopic spot holds a short, synthetic, single-stranded DNA sequence from ...
TRANSPORT PROCESSES
... signal sequence is cleaved from the protein while it is still growing on the ribosome; thus, signal sequences are usually not present in the “mature” proteins found in cells. The hydrophobic residues in the core of ER signal sequences form a binding site that is critical for the interaction of signa ...
... signal sequence is cleaved from the protein while it is still growing on the ribosome; thus, signal sequences are usually not present in the “mature” proteins found in cells. The hydrophobic residues in the core of ER signal sequences form a binding site that is critical for the interaction of signa ...
Interactions, Tertiary Structures
... Fibrous Proteins, α- and β-Keratins Fibrous proteins consist of long, thin, fiber-like shapes, involved in the structure of cells and tissues. Two types of fibrous protein include • α-keratins, which make up hair, wool, skin, and nails and contain three α-helices linked by disulfide — S — S — linka ...
... Fibrous Proteins, α- and β-Keratins Fibrous proteins consist of long, thin, fiber-like shapes, involved in the structure of cells and tissues. Two types of fibrous protein include • α-keratins, which make up hair, wool, skin, and nails and contain three α-helices linked by disulfide — S — S — linka ...
Powerpoint
... Poole, L. B., Zeng, B. B., Knaggs, S. A., Yakubu, M. and King, S. B. (2005) Synthesis of chemical probes to map sulfenic acid modifications on proteins. Bioconjug Chem 16, 1624-16028. Example of the strategies to develop a thiol tag that can be applied to proteomics. Landar, A., Oh, J. Y., Giles, N. ...
... Poole, L. B., Zeng, B. B., Knaggs, S. A., Yakubu, M. and King, S. B. (2005) Synthesis of chemical probes to map sulfenic acid modifications on proteins. Bioconjug Chem 16, 1624-16028. Example of the strategies to develop a thiol tag that can be applied to proteomics. Landar, A., Oh, J. Y., Giles, N. ...
Analitical chemistry 1
... analysis will depend on the information desired. In the case of biological fluids, the conditions under which the sample is collected can be important. For example, whether a patient has just eaten. Because the composition of blood varies before and after meals and for many analyses a sample is coll ...
... analysis will depend on the information desired. In the case of biological fluids, the conditions under which the sample is collected can be important. For example, whether a patient has just eaten. Because the composition of blood varies before and after meals and for many analyses a sample is coll ...
Ion Exchange Chromatography
... Ion exchange chromatography retains analyte molecules based on ionic interactions. The stationary phase surface displays ionic functional groups (R-X) that interact with analyte ions of opposite charge. This type of chromatography is further subdivided ...
... Ion exchange chromatography retains analyte molecules based on ionic interactions. The stationary phase surface displays ionic functional groups (R-X) that interact with analyte ions of opposite charge. This type of chromatography is further subdivided ...
PowerPoint Presentation from June
... Glycosylated proteins show high resistance to proteases; certain post-translational modifications (e.g. phosphorylation) decrease the detectability of the modified peptide using the standard protein mass spectrometry techniques. ...
... Glycosylated proteins show high resistance to proteases; certain post-translational modifications (e.g. phosphorylation) decrease the detectability of the modified peptide using the standard protein mass spectrometry techniques. ...
Central Dogma - essentiavitae.com
... Stop codon – UGA, UAA, and UAG on mRNA; amino acids that signal the end of the synthesis of a protein RNA – ribonucleic acid, a nucleic acid whose bases are A, C, U, and G mRNA – messenger RNA; a molecule of RNA complementary in sequence to the template strand of a gene that specifies a protein ...
... Stop codon – UGA, UAA, and UAG on mRNA; amino acids that signal the end of the synthesis of a protein RNA – ribonucleic acid, a nucleic acid whose bases are A, C, U, and G mRNA – messenger RNA; a molecule of RNA complementary in sequence to the template strand of a gene that specifies a protein ...
QPrEST™—isotope-labeled multipeptide standards for
... recombinant proteins expressed in a bacterial host with >99% isotopic incorporation and precisely determined concentrations. Each standard contains a stretch of a human protein including at least two unique tryptic peptides, ensuring multiple data points from a single standard. Furthermore, the QPrE ...
... recombinant proteins expressed in a bacterial host with >99% isotopic incorporation and precisely determined concentrations. Each standard contains a stretch of a human protein including at least two unique tryptic peptides, ensuring multiple data points from a single standard. Furthermore, the QPrE ...
Chapt. 10: Protein Sorting, Transport: Endoplasmic Reticulum, Golgi
... Posttranslation translocation (more common in yeast): • Proteins synthesized on free ribosomes • Signal sequences recognized by receptors on translocon (not need SRP) • Hsp70 chaperones keep polypeptide chains unfolded so can enter translocon • Hsp70 chaperone in ER (BiP) acts as ratchet to pull pol ...
... Posttranslation translocation (more common in yeast): • Proteins synthesized on free ribosomes • Signal sequences recognized by receptors on translocon (not need SRP) • Hsp70 chaperones keep polypeptide chains unfolded so can enter translocon • Hsp70 chaperone in ER (BiP) acts as ratchet to pull pol ...
ppt
... • Proteins from ER are processed and sorted for transport: to endosomes, lysosomes, plasma membrane, or secretion. • Most glycolipids and sphingomyelin are made in Golgi, • The Golgi is composed of flattened membrane-enclosed sacs (cisternae) and associated vesicles: ...
... • Proteins from ER are processed and sorted for transport: to endosomes, lysosomes, plasma membrane, or secretion. • Most glycolipids and sphingomyelin are made in Golgi, • The Golgi is composed of flattened membrane-enclosed sacs (cisternae) and associated vesicles: ...
TUTORIAL FOR PROTEIN TECHNOLOGY: Ion-exchange
... In the diagram, one of the non-polar solutes is an attached ligand. The other, the protein, when it interacts with this,will also be bound to the column via the ligand. In other circumstances, the protein will start to precipitate out of solution( for example, ammonium sulphate precipitation). Take ...
... In the diagram, one of the non-polar solutes is an attached ligand. The other, the protein, when it interacts with this,will also be bound to the column via the ligand. In other circumstances, the protein will start to precipitate out of solution( for example, ammonium sulphate precipitation). Take ...
Genome Organisation Non-coding RNA
... between 21 to 25 nucleotides long. These are very recently discovered RNA species and there is intense interest in the literature at present concerning the functions of these molecules. They are seen as the next anti-viral agents, cures for cancer etc even a replacement for fossil fuels!!! (well not ...
... between 21 to 25 nucleotides long. These are very recently discovered RNA species and there is intense interest in the literature at present concerning the functions of these molecules. They are seen as the next anti-viral agents, cures for cancer etc even a replacement for fossil fuels!!! (well not ...
The genome organisation: the C-value paradox
... between 21 to 25 nucleotides long. These are very recently discovered RNA species and there is intense interest in the literature at present concerning the functions of these molecules. They are seen as the next anti-viral agents, cures for cancer etc even a replacement for fossil fuels!!! (well not ...
... between 21 to 25 nucleotides long. These are very recently discovered RNA species and there is intense interest in the literature at present concerning the functions of these molecules. They are seen as the next anti-viral agents, cures for cancer etc even a replacement for fossil fuels!!! (well not ...
Prolyl Isomerases –Old Proteins as New Therapeutic Targets
... The early pioneering work by Fischer concerned the enzymology of protein folding, a biochemical event unlikely to show up in the massive “target identification and validation” efforts that have taken place in pharmaceutical and biotechnology companies over the last 20 years. Moreover, a publication ...
... The early pioneering work by Fischer concerned the enzymology of protein folding, a biochemical event unlikely to show up in the massive “target identification and validation” efforts that have taken place in pharmaceutical and biotechnology companies over the last 20 years. Moreover, a publication ...
Primary Structure of Diphtheria Toxin Fragment B
... The second lipid-associating domain of fragment B, located in its middle region, is structurally related to the membranous segments of integral membrane proteins and designed to interact by its large apolar face with the hydrocarbon core of the phospholipids. This highly hydrophobic region of B coul ...
... The second lipid-associating domain of fragment B, located in its middle region, is structurally related to the membranous segments of integral membrane proteins and designed to interact by its large apolar face with the hydrocarbon core of the phospholipids. This highly hydrophobic region of B coul ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.