02_Murray - Sbkb.org
... with in vitro binding measurements and subcellular localization: Residue substitution of a single family member ...
... with in vitro binding measurements and subcellular localization: Residue substitution of a single family member ...
CANADIAN TRANSLATION OF FISHERIES AND AQUATIC
... Results -a. SEXUAL DIMORPHISM IN SALMON Figure 1 shows that -Unit,- the proteinogram of the sexually mature female may be distinguished from that of either the very young (parr) or spawning (adult) male by means of electrophoresis in gelose. ...
... Results -a. SEXUAL DIMORPHISM IN SALMON Figure 1 shows that -Unit,- the proteinogram of the sexually mature female may be distinguished from that of either the very young (parr) or spawning (adult) male by means of electrophoresis in gelose. ...
Proteomics of spermatogenesis: from protein lists to understanding
... samples via high throughput technology designed to generate a global view of the organism’s biology. The latter is a dynamic proteome and provides a comparative view of different biological processes, which allows a better understanding of the physiological and pathological states of an organism. Th ...
... samples via high throughput technology designed to generate a global view of the organism’s biology. The latter is a dynamic proteome and provides a comparative view of different biological processes, which allows a better understanding of the physiological and pathological states of an organism. Th ...
MINI REVIEW Lectin-like proteins in model organisms: implications
... 1998). Lectins are often complex, multidomain proteins, but sugar-binding activity can usually be ascribed to a single protein module within the lectin polypeptide. Such a module is designated a carbohydrate-recognition domain (CRD). CRDs in vertebrate lectins fall into a number of structurally dist ...
... 1998). Lectins are often complex, multidomain proteins, but sugar-binding activity can usually be ascribed to a single protein module within the lectin polypeptide. Such a module is designated a carbohydrate-recognition domain (CRD). CRDs in vertebrate lectins fall into a number of structurally dist ...
Lesson Overview - Midland Park School
... molecule). It is known as a codon. A codon consists of three consecutive bases that specify a single amino acid to be added to the polypeptide chain. ...
... molecule). It is known as a codon. A codon consists of three consecutive bases that specify a single amino acid to be added to the polypeptide chain. ...
![[] Protein Splicing i) inteins and ext...,](http://s1.studyres.com/store/data/008277893_1-250b6a85b20526696d229e05c4a3b4d7-300x300.png)
Electrophoresis
... in solution, chiefly proteins and nucleic acids, migrate in response to an electrical field. Their rate of migration through the electrical field, depends on the strength of the field, on the net charge, size, and shape of the molecules, and also on the ionic strength, viscosity, and temperature of ...
... in solution, chiefly proteins and nucleic acids, migrate in response to an electrical field. Their rate of migration through the electrical field, depends on the strength of the field, on the net charge, size, and shape of the molecules, and also on the ionic strength, viscosity, and temperature of ...
Sourirajan, Jayanthi: Protein Structure Prediction
... interactions, study of evolutionary relationship between proteins or protein families etc. The 3D structure of proteins can be solved by 1) Experimental methods, or 2) Structure prediction. Solving structures experimentally is very hard. Solving through X-ray crystallography produces very good resul ...
... interactions, study of evolutionary relationship between proteins or protein families etc. The 3D structure of proteins can be solved by 1) Experimental methods, or 2) Structure prediction. Solving structures experimentally is very hard. Solving through X-ray crystallography produces very good resul ...
Supplementary information
... harvested and resuspended in SDS-lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.1) and sonicated to yield DNA fragments with lengths ranging from 200 bp to 1000 bp. After centrifugation, the lysates were precleared with protein A resin at 4C for 1 h and immunoprecipitated with anti-His anti ...
... harvested and resuspended in SDS-lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.1) and sonicated to yield DNA fragments with lengths ranging from 200 bp to 1000 bp. After centrifugation, the lysates were precleared with protein A resin at 4C for 1 h and immunoprecipitated with anti-His anti ...
Interpro - European Bioinformatics Institute
... By uniting the member databases, InterPro capitalises on their individual strengths, producing a powerful diagnostic tool & integrated database ...
... By uniting the member databases, InterPro capitalises on their individual strengths, producing a powerful diagnostic tool & integrated database ...
FoldNucleus: web server for the prediction of RNA
... 3 The FoldNucleus server The FoldNucleus webserver is available at http://bioinfo.protres.ru/ foldnucleus/. For the prediction of A values using this server, one should specify RNA or a protein structure for which the prediction is to be made. For this purpose, one should specify the corresponding P ...
... 3 The FoldNucleus server The FoldNucleus webserver is available at http://bioinfo.protres.ru/ foldnucleus/. For the prediction of A values using this server, one should specify RNA or a protein structure for which the prediction is to be made. For this purpose, one should specify the corresponding P ...
Supplementary Information (doc 48K)
... of serine, threonine and tyrosine (Ser/Thr/Tyr +79.966331 Da) were searched as variable ...
... of serine, threonine and tyrosine (Ser/Thr/Tyr +79.966331 Da) were searched as variable ...
2-D gels and protein mass spectrometry
... number of carbon atoms, few molecules will contain any heavier isotopes of C (or most other elements). The difference in mass between M and M+1 is a significant fraction of M, so the peaks are easily resolved by the spectrometer. But proteins contain hundreds or even thousands of carbon atoms, so al ...
... number of carbon atoms, few molecules will contain any heavier isotopes of C (or most other elements). The difference in mass between M and M+1 is a significant fraction of M, so the peaks are easily resolved by the spectrometer. But proteins contain hundreds or even thousands of carbon atoms, so al ...
Nucleolar translocalization of GRA10 of Toxoplasma gondii
... µg/ml) and high dose (0.5 µg/ml) of actinomycin D (Sigma) as an inhibitor of RNA polymerase I and 1 to 40 µ g/ml of α -amanitin as a RNA polymerase II (Dundr et al., 1995). ...
... µg/ml) and high dose (0.5 µg/ml) of actinomycin D (Sigma) as an inhibitor of RNA polymerase I and 1 to 40 µ g/ml of α -amanitin as a RNA polymerase II (Dundr et al., 1995). ...
Chlamydomonas reinhardtii strains carrying the stb1-1
... The original mutant strains I73 (sta4-1) and I97 (sta4-2) were transformed with the complete PHOB genomic fragment amplified by PCR and cloned in the pSL18 plasmid (see methods). The paromomycin resistant clones were screened by zymogram (a). Three complementated strains were obtained from 23 resist ...
... The original mutant strains I73 (sta4-1) and I97 (sta4-2) were transformed with the complete PHOB genomic fragment amplified by PCR and cloned in the pSL18 plasmid (see methods). The paromomycin resistant clones were screened by zymogram (a). Three complementated strains were obtained from 23 resist ...
Expression and Purification of Functional Ligand
... To quantify the interaction of sweet ligands with the NTD of T1Rs, it was necessary to design an expression and purification protocol that would permit the isolation of purified, soluble T1R NTD proteins in sufficient quantities for biochemical studies. We took advantage of two bacterial expression ...
... To quantify the interaction of sweet ligands with the NTD of T1Rs, it was necessary to design an expression and purification protocol that would permit the isolation of purified, soluble T1R NTD proteins in sufficient quantities for biochemical studies. We took advantage of two bacterial expression ...
Purification of GST::TaABF1 Fusion Protein in Order to Assess its
... Transcription factor TaABF1, a member of the ABA response element binding factor family, has been shown to have an important role in the signaling pathways of gibberellin (GA) and abscisic acid (ABA) in cereal grains. TaABF1 has also been found to be phosphorylated in vivo in aleurone cells and is p ...
... Transcription factor TaABF1, a member of the ABA response element binding factor family, has been shown to have an important role in the signaling pathways of gibberellin (GA) and abscisic acid (ABA) in cereal grains. TaABF1 has also been found to be phosphorylated in vivo in aleurone cells and is p ...
CSCI 474 Lab 4a : inferring the effects of mutations Spring 2017
... (try to) infer the effect of a mutation using only the sequence of amino acids in a protein. Such tools have varying degrees of accuracy for the reason that the effect of a mutation is dependent on how the amino acid substitution/deletion/insertion affects the 3D structure of the protein, and not ju ...
... (try to) infer the effect of a mutation using only the sequence of amino acids in a protein. Such tools have varying degrees of accuracy for the reason that the effect of a mutation is dependent on how the amino acid substitution/deletion/insertion affects the 3D structure of the protein, and not ju ...
Poly(Pro)II Helices in Globular Proteins: Identification and Circular
... arranged as in one turn of a PIIhelix, which has implications for exciton interactions and the resulting C D spectra. However, whether isolated residues assigned to the PIIstructure need to be considered as PIIor left unassigned is not clear. A single residue in the PIIconformation cannot be conside ...
... arranged as in one turn of a PIIhelix, which has implications for exciton interactions and the resulting C D spectra. However, whether isolated residues assigned to the PIIstructure need to be considered as PIIor left unassigned is not clear. A single residue in the PIIconformation cannot be conside ...
Molecular Chaperones in the Cytosol: from Nascent Chain to Folded
... residues (27). The nascent chain–TF complex dissociates, in an ATP-independent manner, after chain release from the ribosome (27). Although TF exhibits peptidyl-prolyl cis/trans isomerase (PPIase) activity in vitro, recognition of target polypeptides by TF is independent of proline residues (28) and ...
... residues (27). The nascent chain–TF complex dissociates, in an ATP-independent manner, after chain release from the ribosome (27). Although TF exhibits peptidyl-prolyl cis/trans isomerase (PPIase) activity in vitro, recognition of target polypeptides by TF is independent of proline residues (28) and ...
ppt - University of Illinois Urbana
... structure patterns of the family members to reveal shared characteristics that potentially describe common biological properties – Multiple sequence alignment – Motif/Domain - sequence and/or structure patterns common to protein family members (a trait) – Profile is a way to represent a family of pr ...
... structure patterns of the family members to reveal shared characteristics that potentially describe common biological properties – Multiple sequence alignment – Motif/Domain - sequence and/or structure patterns common to protein family members (a trait) – Profile is a way to represent a family of pr ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.