Structure, function and evolution of armless mitochondrial tRNAs
... were investigated. First, the structure of two tRNAs was analyzed by means of enzymatic and chemical probing, nuclear magnetic resonance spectroscopy (NMR), and small angle X-ray scattering (SAXS). The results of these analysis show that armless tRNAs form a hairpin-like secondary structure composed ...
... were investigated. First, the structure of two tRNAs was analyzed by means of enzymatic and chemical probing, nuclear magnetic resonance spectroscopy (NMR), and small angle X-ray scattering (SAXS). The results of these analysis show that armless tRNAs form a hairpin-like secondary structure composed ...
The role of cytosolic proteins in the insertion of tail
... nascent chain to the Sec61 protein-conducting channel (Rapoport, 2007). The well characterized co-translational pathway is, however, not accessible to the large and diverse group of membrane proteins that are classified as tail-anchored (TA) proteins. TA proteins consist of a cytosolic N-terminal do ...
... nascent chain to the Sec61 protein-conducting channel (Rapoport, 2007). The well characterized co-translational pathway is, however, not accessible to the large and diverse group of membrane proteins that are classified as tail-anchored (TA) proteins. TA proteins consist of a cytosolic N-terminal do ...
Chapter 5 The Structure and Function of Large Biological Molecules
... It is hard to predict a protein’s structure from its primary structure Most proteins probably go through several stages on their way to a stable structure Chaperonins are protein molecules that assist the proper folding of other proteins Normal protein ...
... It is hard to predict a protein’s structure from its primary structure Most proteins probably go through several stages on their way to a stable structure Chaperonins are protein molecules that assist the proper folding of other proteins Normal protein ...
1408 Chapter 3.key
... – They can be sold as prescription drugs and used to treat certain diseases – They may also be abused with serious consequences, such as liver damage that can lead to cancer ...
... – They can be sold as prescription drugs and used to treat certain diseases – They may also be abused with serious consequences, such as liver damage that can lead to cancer ...
Data-driven docking for the study of biomolecular complexes
... Computational Structural Biology, Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University, 3584CH, Utrecht, The Netherlands. Email: [email protected] With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particula ...
... Computational Structural Biology, Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University, 3584CH, Utrecht, The Netherlands. Email: [email protected] With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particula ...
Histidine protonation and the activation of viral fusion proteins
... Experimentally, protein-mediated membrane fusion cannot be observed with either the spatial or temporal resolution required to investigate the role of specific histidine residues. Instead, to selectively test the effect of histidine protonation, MD simulations have been performed of the DEN2 sE prot ...
... Experimentally, protein-mediated membrane fusion cannot be observed with either the spatial or temporal resolution required to investigate the role of specific histidine residues. Instead, to selectively test the effect of histidine protonation, MD simulations have been performed of the DEN2 sE prot ...
Activity: Poisonous mushrooms worksheet
... This is the basic structure but it would be more useful if students set this out as shown in the PowerPoint presentation. This makes it easier to show how two amino acids are joined by condensation. (b) Peptide bond correctly shown between the COOH and NH2; (1 mark) For further clarification see Pow ...
... This is the basic structure but it would be more useful if students set this out as shown in the PowerPoint presentation. This makes it easier to show how two amino acids are joined by condensation. (b) Peptide bond correctly shown between the COOH and NH2; (1 mark) For further clarification see Pow ...
Computational Methods for Exploration and Analysis of
... plays a major role, which has recently been recognized by the Nobel Committee [1]. The energy landscape underscores the inherent nature of biomolecules, which are dynamical objects that are always interconverting between structures with varying energies. It affirms that biomolecules must be describe ...
... plays a major role, which has recently been recognized by the Nobel Committee [1]. The energy landscape underscores the inherent nature of biomolecules, which are dynamical objects that are always interconverting between structures with varying energies. It affirms that biomolecules must be describe ...
PDF File
... emerging understanding of the relationships between enzyme superfamilies, and Khersonsky, Roodveldt and Tawfik focus on a particularly fascinating aspect of evolutionary relationships, properties of ‘catalytic promiscuity’ — the low level of activity of an enzyme for a reaction other than its curren ...
... emerging understanding of the relationships between enzyme superfamilies, and Khersonsky, Roodveldt and Tawfik focus on a particularly fascinating aspect of evolutionary relationships, properties of ‘catalytic promiscuity’ — the low level of activity of an enzyme for a reaction other than its curren ...
Protein - Creating Vitality
... our bodies, and half of our dry body weight (water taken out) is made of protein. More protein facts: All organs, muscles, tendons, cartilage, bones and blood vessels depend upon adequate protein supplies in order to maintain their structure and function. Every cell in the body is held together ...
... our bodies, and half of our dry body weight (water taken out) is made of protein. More protein facts: All organs, muscles, tendons, cartilage, bones and blood vessels depend upon adequate protein supplies in order to maintain their structure and function. Every cell in the body is held together ...
insulin-like growth factor binding proteins and their functions
... Posttranslation modifications of IGFBPs can influence their biological activity. These modifications of IGFBPs such as phosphorylation result in the change of their affinity to IGFs. More phosphorylated form of IGFBP-1 of human amniotic fluid and decidua has higher binding affinity to IGFs than less ...
... Posttranslation modifications of IGFBPs can influence their biological activity. These modifications of IGFBPs such as phosphorylation result in the change of their affinity to IGFs. More phosphorylated form of IGFBP-1 of human amniotic fluid and decidua has higher binding affinity to IGFs than less ...
Architecture of the trypanosome RNA editing accessory complex
... Both MRB8180/MRB4150 and MRB8170/MRB4160 are the result of a chromosomal duplication in T. brucei (but not L. major), and therefore these two proteins have the same single L. major homolog. g Some of the predicted sizes for the T. brucei proteins are different from those listed on TriTrypDB because ...
... Both MRB8180/MRB4150 and MRB8170/MRB4160 are the result of a chromosomal duplication in T. brucei (but not L. major), and therefore these two proteins have the same single L. major homolog. g Some of the predicted sizes for the T. brucei proteins are different from those listed on TriTrypDB because ...
Assembly and function of cell surface structures of the
... with total A. ambivalens RNA in order to confirm the transcription start site independently, to map the transcriptional terminators, to demonstrate that the internal transcription terminator is active, and to show that the 3000 nt transcript resulted from a true mRNA but not from unspecific mRNA tra ...
... with total A. ambivalens RNA in order to confirm the transcription start site independently, to map the transcriptional terminators, to demonstrate that the internal transcription terminator is active, and to show that the 3000 nt transcript resulted from a true mRNA but not from unspecific mRNA tra ...
Lysine Acetylation - Regulator of Diverse Cellular Processes
... Lysine Acetylation - Regulator of Diverse Cellular Processes Lysine acetylation is a post-translational modification (PTM) crucial for regulating the function and localization of many eukaryotic proteins. This PTM is reversible, regulated by histone deacetylases (HDACs) and histone acetyltransferase ...
... Lysine Acetylation - Regulator of Diverse Cellular Processes Lysine acetylation is a post-translational modification (PTM) crucial for regulating the function and localization of many eukaryotic proteins. This PTM is reversible, regulated by histone deacetylases (HDACs) and histone acetyltransferase ...
Resonance-assignement-Structure-constraints
... The receiver coil picks up the signal from the sample. An analog-to-digital converter “reads” the voltage and sends it to the computer for data storage. ...
... The receiver coil picks up the signal from the sample. An analog-to-digital converter “reads” the voltage and sends it to the computer for data storage. ...
Publications_files/Stotland et al 2012
... FACS-sorted, clonally selected on the basis of high mCherry expression, and probed with anti-CSN1 antibody to confirm the expression of SBP-CSN1 (Fig .1B–D). As a control, we utilized a similar construct where CSN1 was substituted with Citrine, a modified yellow fluorescent protein chosen to exclude ...
... FACS-sorted, clonally selected on the basis of high mCherry expression, and probed with anti-CSN1 antibody to confirm the expression of SBP-CSN1 (Fig .1B–D). As a control, we utilized a similar construct where CSN1 was substituted with Citrine, a modified yellow fluorescent protein chosen to exclude ...
Sato Talk
... while the origin of mitochondria is likely to be an ancestor of alpha proteobacteria. This inference is mainly supported by the comparison of genomic sequences of the organelles and various bacteria. However, we recently pointed out that, based on accumulating database information and analytical dat ...
... while the origin of mitochondria is likely to be an ancestor of alpha proteobacteria. This inference is mainly supported by the comparison of genomic sequences of the organelles and various bacteria. However, we recently pointed out that, based on accumulating database information and analytical dat ...
294_2005_38_MOESM43_ESM - Springer Static Content Server
... in filamentous fungi, which is reflected in the protein size (53995 instead of 30039; p=3.9*10-3, t-test). In contrast to the Saccharomyces, others yeasts only seem to have Ptc1 and Ptc3 orthologues. A. gossypii has a syntenic homologue to Ptc1 and Ptc3 but not to Ptc2. Similarly, filamentous fun ...
... in filamentous fungi, which is reflected in the protein size (53995 instead of 30039; p=3.9*10-3, t-test). In contrast to the Saccharomyces, others yeasts only seem to have Ptc1 and Ptc3 orthologues. A. gossypii has a syntenic homologue to Ptc1 and Ptc3 but not to Ptc2. Similarly, filamentous fun ...
Analysis of Protein Interactions at Native Chloroplast Membranes by
... and mycotoxins with detection levels as low as 0.1 ng/ml [6]. TIRE has also been used to determine the binding affinity of the molecular chaperone Hsp70 for a soluble form of its receptor OEP61 bound to a gold surface, and was sensitive enough to discriminate between the binding affinities of closel ...
... and mycotoxins with detection levels as low as 0.1 ng/ml [6]. TIRE has also been used to determine the binding affinity of the molecular chaperone Hsp70 for a soluble form of its receptor OEP61 bound to a gold surface, and was sensitive enough to discriminate between the binding affinities of closel ...
A High Yield Method for the Removal of Detergents from Low
... mass spectrometric analysis of 2.5-10 ug of BSA enzymatic digests at 25-100 µg/mL prepared in the presence of detergents and processed to remove detergent revealed sequence coverage and MASCOT scores as good as or better than control BSA samples processed without detergent. The method significantly ...
... mass spectrometric analysis of 2.5-10 ug of BSA enzymatic digests at 25-100 µg/mL prepared in the presence of detergents and processed to remove detergent revealed sequence coverage and MASCOT scores as good as or better than control BSA samples processed without detergent. The method significantly ...
2 Nucleic Acids
... Proteins are molecules that have many different functions in living things. All proteins are made of monomers called amino acids (Figure 1.2) that connect together like beads on a necklace (Figure 1.3). There are only 20 common amino acids needed to build proteins. These amino acids form in thousand ...
... Proteins are molecules that have many different functions in living things. All proteins are made of monomers called amino acids (Figure 1.2) that connect together like beads on a necklace (Figure 1.3). There are only 20 common amino acids needed to build proteins. These amino acids form in thousand ...
Plant serine/arginine-rich proteins and their role in pre
... Pre-messenger RNA (pre-mRNA) splicing, a process by which mature mRNAs are generated by excision of introns and ligation of exons, is an important step in the regulation of gene expression in all eukaryotes. Selection of alternative splice sites in a pre-mRNA generates multiple mRNAs from a single g ...
... Pre-messenger RNA (pre-mRNA) splicing, a process by which mature mRNAs are generated by excision of introns and ligation of exons, is an important step in the regulation of gene expression in all eukaryotes. Selection of alternative splice sites in a pre-mRNA generates multiple mRNAs from a single g ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.