Figure S1. Chloroplast localization and topology of TerC
... thylakoid membranes of transformed Arabidopsis lines. Salt treatments were performed as described previously (Armbruster et al. 2010) using antibodies specific for GFP, Lhcb1 (Agrisera) and PsaD (Agrisera). For thermolysin treatments, isolated thylakoid membranes were resuspended in10 mM HEPES-KOH, ...
... thylakoid membranes of transformed Arabidopsis lines. Salt treatments were performed as described previously (Armbruster et al. 2010) using antibodies specific for GFP, Lhcb1 (Agrisera) and PsaD (Agrisera). For thermolysin treatments, isolated thylakoid membranes were resuspended in10 mM HEPES-KOH, ...
Degradation by bacteria of membrane and soluble protein in seawater
... Expt 5 (data not shown). No data of microbial abundances are available for Expts 1, 2, and 3. The time course of protein degradation was described well by a model of first-order kinetics during the entire incubation period in Expt 4 (Fig. 1B) and other experiments, except that a lag period was obser ...
... Expt 5 (data not shown). No data of microbial abundances are available for Expts 1, 2, and 3. The time course of protein degradation was described well by a model of first-order kinetics during the entire incubation period in Expt 4 (Fig. 1B) and other experiments, except that a lag period was obser ...
Proteins - virtual laboratories
... language of polypeptides, which consists of sequences of the 20 different amino acids. As pointed out by the physicist George Gamow (1904-1968), when he was a professor at UC Boulder, the minimum set of nucleotides needed to encode all 20 amino acids is three; a sequence of one nucleotide (41) could ...
... language of polypeptides, which consists of sequences of the 20 different amino acids. As pointed out by the physicist George Gamow (1904-1968), when he was a professor at UC Boulder, the minimum set of nucleotides needed to encode all 20 amino acids is three; a sequence of one nucleotide (41) could ...
ID helix-loop-helix proteins - Journal of Cell Science
... relatives, in the ‘molecular clock’ mechanism controlling the timing of oligodendrocyte differentiation. The above studies clearly illustrate the complex role played by ID proteins in mammalian cell fate determination. Numerous reports show that ID proteins function as negative regulators of differe ...
... relatives, in the ‘molecular clock’ mechanism controlling the timing of oligodendrocyte differentiation. The above studies clearly illustrate the complex role played by ID proteins in mammalian cell fate determination. Numerous reports show that ID proteins function as negative regulators of differe ...
Protein-protein interaction networks (I): data preprocessing
... When assessing the quality of interaction data, the coverage and the accuracy are needed to be considered together. A data set of high coverage is not very useful if its accuracy is low, i.e. if it contains a large number of false positives.Similarly, a data set of high accuracy is not very useful i ...
... When assessing the quality of interaction data, the coverage and the accuracy are needed to be considered together. A data set of high coverage is not very useful if its accuracy is low, i.e. if it contains a large number of false positives.Similarly, a data set of high accuracy is not very useful i ...
Table S1.
... The enzymatic activity of all proteins was compared to the activity of wtSOD1 and a commercial recombinant SOD1 protein (hucSOD1). Of the mutant SOD1 purified, three have mutations located away from the metal binding/catalytic region, thus having properties similar to wtSOD1. These mutants are denot ...
... The enzymatic activity of all proteins was compared to the activity of wtSOD1 and a commercial recombinant SOD1 protein (hucSOD1). Of the mutant SOD1 purified, three have mutations located away from the metal binding/catalytic region, thus having properties similar to wtSOD1. These mutants are denot ...
Bacterial ancestry of actin and tubulin Fusinita van den Ent, Linda
... shall focus on tubulin and actin and their putative homologues in prokaryotic cells. The eukaryotic cytoskeleton is not a static structure. The polymers of the cytoskeleton are highly dynamic, allowing the cytoskeleton to rapidly re-organise. Owing to the polymerisation dynamics, the polymers have t ...
... shall focus on tubulin and actin and their putative homologues in prokaryotic cells. The eukaryotic cytoskeleton is not a static structure. The polymers of the cytoskeleton are highly dynamic, allowing the cytoskeleton to rapidly re-organise. Owing to the polymerisation dynamics, the polymers have t ...
RLIMS-P Annotation guidelines This document describes some
... deficits in a wide variety of animal models of CNS diseases, including Alzheimer's disease, Fragile X syndrome, Down syndrome, Parkinson's disease, spinocerebellar ataxia type 1, traumatic brain injury, and others.” PMID: 23916593. “cGMP-dependent protein kinase II (cGKII; encoded by PRKG2) is a ser ...
... deficits in a wide variety of animal models of CNS diseases, including Alzheimer's disease, Fragile X syndrome, Down syndrome, Parkinson's disease, spinocerebellar ataxia type 1, traumatic brain injury, and others.” PMID: 23916593. “cGMP-dependent protein kinase II (cGKII; encoded by PRKG2) is a ser ...
Identification of a novel human ... through its interaction with the ...
... specificity and modes of receptor recruitment. Grb7 binds via its SH2 domain to a variety of RTKs and tyrosine phosphorylated proteins, including erbB2, erbB3 and Shc (7-10). In the case of Grb10, most attention has focused on its recruitment by the IR and IGF-1R (7,11). Grb14 is also bound by the I ...
... specificity and modes of receptor recruitment. Grb7 binds via its SH2 domain to a variety of RTKs and tyrosine phosphorylated proteins, including erbB2, erbB3 and Shc (7-10). In the case of Grb10, most attention has focused on its recruitment by the IR and IGF-1R (7,11). Grb14 is also bound by the I ...
Post-Transcriptional Regulation of Gene Expression: the Many Hats
... contiguous, whereas in larger elements one would anticipate that the 3D structure brings together nucleotides separated in the linear sequence into the binding site. This hypothesis, and the ability of proteins to recognise even the A2RE11 cis-acting element with a range of point mutations (8), make ...
... contiguous, whereas in larger elements one would anticipate that the 3D structure brings together nucleotides separated in the linear sequence into the binding site. This hypothesis, and the ability of proteins to recognise even the A2RE11 cis-acting element with a range of point mutations (8), make ...
emboj2009256-sup
... Alignment of sequences from RISP homologues Arabidopsis (accession numbers NM_125513.1 and NP_196406), Vitus vinifera (accession number CAO64487.1) and Oryza sativa (accession number XP_479024) available in the database. Conserved amino acids are shown in black. Domains, where computer analysis pred ...
... Alignment of sequences from RISP homologues Arabidopsis (accession numbers NM_125513.1 and NP_196406), Vitus vinifera (accession number CAO64487.1) and Oryza sativa (accession number XP_479024) available in the database. Conserved amino acids are shown in black. Domains, where computer analysis pred ...
Improved amino acid analysis of feedstuffs
... measurements. Chromatographic control, detection, and safety systems were performed via a PC with fully integrated software. The ninhydrin flow rate was set at 25 ml/hr and the buffer flow rate at 35 ml/hr. The system operated using a novel buffer chemistry formulated for this application which is c ...
... measurements. Chromatographic control, detection, and safety systems were performed via a PC with fully integrated software. The ninhydrin flow rate was set at 25 ml/hr and the buffer flow rate at 35 ml/hr. The system operated using a novel buffer chemistry formulated for this application which is c ...
GELBANK: a database of annotated two
... web interface. The proteomes can be queried and the resulting data set can be used as an input for some proteomics tools that are pertinent to 2D gel electrophoresis. A new `shopping basket' is introduced (Bio-bag) that allows the storage of objects presented on the website. Gelbank's content can be ...
... web interface. The proteomes can be queried and the resulting data set can be used as an input for some proteomics tools that are pertinent to 2D gel electrophoresis. A new `shopping basket' is introduced (Bio-bag) that allows the storage of objects presented on the website. Gelbank's content can be ...
Part 2
... shown. Next, the downward arrows must appear along with for protein separation. These separated text followed by the blue bands on the left and the spots on proteins on the gel are used for further ...
... shown. Next, the downward arrows must appear along with for protein separation. These separated text followed by the blue bands on the left and the spots on proteins on the gel are used for further ...
Full-Text PDF
... inflammation is the most important part in order to discover effective and specific antibiotics for H. pylori. In this regard, the structural and functional study of H. pylori proteins should be the most important point for developing antibiotics and can provide clues to help cope with new antibioti ...
... inflammation is the most important part in order to discover effective and specific antibiotics for H. pylori. In this regard, the structural and functional study of H. pylori proteins should be the most important point for developing antibiotics and can provide clues to help cope with new antibioti ...
Immunoturbidimetric assay
... AGE Production • Non-enzymatic glycosylation between a free amino group on a protein and a carboxyl group from a sugar e.g. glucose to form Schiff’s base • Schiff base formation is relatively fast and highly reversible and is an equilibrium reaction. (The amount of Schiff base formed is dictated b ...
... AGE Production • Non-enzymatic glycosylation between a free amino group on a protein and a carboxyl group from a sugar e.g. glucose to form Schiff’s base • Schiff base formation is relatively fast and highly reversible and is an equilibrium reaction. (The amount of Schiff base formed is dictated b ...
Detonator of the population explosion
... of the steel industry. It should be risked.” And he did it: he solved some unprecedented engineering problems, and commercial production of ammonia began on 9 September 1913, just four years and two months after Haber’s laboratory demonstration. Today’s ammonia synthesis has been improved in many de ...
... of the steel industry. It should be risked.” And he did it: he solved some unprecedented engineering problems, and commercial production of ammonia began on 9 September 1913, just four years and two months after Haber’s laboratory demonstration. Today’s ammonia synthesis has been improved in many de ...
putative mineral-specific proteins synthesized by a
... sterilized air. Anaerobic growth was performed in a similar manner with the following exceptions: the medium was made anaerobic by first boiling the MilliQ water used to prepare the medium and then purging the prepared media for 20 to 30 minutes with filter (0.2 m) sterilized oxygen free N2:CO2 (95 ...
... sterilized air. Anaerobic growth was performed in a similar manner with the following exceptions: the medium was made anaerobic by first boiling the MilliQ water used to prepare the medium and then purging the prepared media for 20 to 30 minutes with filter (0.2 m) sterilized oxygen free N2:CO2 (95 ...
Bilayers as Protein Solvents: Role of Bilayer Structure and Elastic
... (J. Gen. Physiol. 129:351–377) deal with several key issues in membrane biology. The four informative papers (MacCallum et al., 2007; von Heijne, 2007; White, 2007; Wolfenden, 2007) obtain detailed quantitative data on the interaction of protein amino acid residues with different membrane models. Se ...
... (J. Gen. Physiol. 129:351–377) deal with several key issues in membrane biology. The four informative papers (MacCallum et al., 2007; von Heijne, 2007; White, 2007; Wolfenden, 2007) obtain detailed quantitative data on the interaction of protein amino acid residues with different membrane models. Se ...
novel 4E-interacting protein in Leishmania is involved in stage
... subunit. eIF4G also interacts with the Poly(A)-Binding Protein (PABP) at the 30 -end of the mRNA, allowing a transient circularization of the mRNA (1). Assembly of the cap-binding complex can be globally regulated by the eIF4E-binding protein, 4E-BP, which is a conserved, small and mostly unstructur ...
... subunit. eIF4G also interacts with the Poly(A)-Binding Protein (PABP) at the 30 -end of the mRNA, allowing a transient circularization of the mRNA (1). Assembly of the cap-binding complex can be globally regulated by the eIF4E-binding protein, 4E-BP, which is a conserved, small and mostly unstructur ...
Global absolute quantification reveals tight regulation of protein
... antibodies to which heavy metals are coupled. This method currently allows quantifying up to 32 cellular proteins at the single-cell level in a single experiment (11,12). During the last decade, mass spectrometry-based proteomics emerged as a powerful tool to study the cellular proteome in an unbias ...
... antibodies to which heavy metals are coupled. This method currently allows quantifying up to 32 cellular proteins at the single-cell level in a single experiment (11,12). During the last decade, mass spectrometry-based proteomics emerged as a powerful tool to study the cellular proteome in an unbias ...
09_chapter 4
... the packing material is suspended in buffer, the charged groups become loosely associated with ions of the opposite charge. Since the buffer contains NaCl that dissociates into Na+ and Cl-, the loosely bound ions are called mobile counterions ((Walker, J.M.; 2005) Ion exchange chromatography separat ...
... the packing material is suspended in buffer, the charged groups become loosely associated with ions of the opposite charge. Since the buffer contains NaCl that dissociates into Na+ and Cl-, the loosely bound ions are called mobile counterions ((Walker, J.M.; 2005) Ion exchange chromatography separat ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.