
Solid Tumour Section Kidney: t(X;17)(p11.2;q23) in renal cell carcinoma
... Antigen (EMA). Underexpression of common epithelial proteins is a typical feature of Xp11.2translocation carcinomas. Surprisingly, the tumor was focally immunoreactive for the melanocytic proteins Melan-A and HMB45 but IHC assays for MiTF and S100 protein were negative. While unusual, the ...
... Antigen (EMA). Underexpression of common epithelial proteins is a typical feature of Xp11.2translocation carcinomas. Surprisingly, the tumor was focally immunoreactive for the melanocytic proteins Melan-A and HMB45 but IHC assays for MiTF and S100 protein were negative. While unusual, the ...
Gene Section SMAP1 (stromal membrane-associated protein 1) Atlas of Genetics and Cytogenetics
... SMAP1 protein is detected in the cytoplasm of cells, and appears to be highly concentrated near the plasma membrane. ...
... SMAP1 protein is detected in the cytoplasm of cells, and appears to be highly concentrated near the plasma membrane. ...
What is a yeast two hybrid assay? How has this approach been
... Reporter transcript Known protein as bait cDNA library If interact, reporter transcript activated Positive vs Negative Selection ...
... Reporter transcript Known protein as bait cDNA library If interact, reporter transcript activated Positive vs Negative Selection ...
Enhancing Sequence Coverage in Proteomics
... common enzyme of choice for proteomics experiments. Digestion with trypsin (or any single enzyme in general) often results in the identification of large numbers of proteins, but sequence coverage is frequently incomplete. If maximum sequence coverage is desired (e.g. when studying changes in protei ...
... common enzyme of choice for proteomics experiments. Digestion with trypsin (or any single enzyme in general) often results in the identification of large numbers of proteins, but sequence coverage is frequently incomplete. If maximum sequence coverage is desired (e.g. when studying changes in protei ...
Figure S1. - BioMed Central
... spectrometry (Orbitrap Velos and Q Exactive), whereas glycopeptides obtained from crude membrane fraction capturing (CMC) were analyzed by low-resolution mass spectrometry (LTQ Velos). Detailed schemes for the LC-MS/MS analysis are shown, including the number of technical replicates and biological r ...
... spectrometry (Orbitrap Velos and Q Exactive), whereas glycopeptides obtained from crude membrane fraction capturing (CMC) were analyzed by low-resolution mass spectrometry (LTQ Velos). Detailed schemes for the LC-MS/MS analysis are shown, including the number of technical replicates and biological r ...
Gene Section SET (SET translocation
... Location: 9q34 Local order: from centromere to telomere: SET, ABL1, NUP214 (alias CAN), NOTCH1 (alias TAN1). ...
... Location: 9q34 Local order: from centromere to telomere: SET, ABL1, NUP214 (alias CAN), NOTCH1 (alias TAN1). ...
Teaching Notes
... Q c. Where are the polar residues located in the structure? Comment about the interaction interfaces between the 4 polymer chains in the structure. A c. The polar residues are distributed all over the surfaces of the beta-barrel structures, except at the interface between pairs of chains A-B and C-D ...
... Q c. Where are the polar residues located in the structure? Comment about the interaction interfaces between the 4 polymer chains in the structure. A c. The polar residues are distributed all over the surfaces of the beta-barrel structures, except at the interface between pairs of chains A-B and C-D ...
Transport of Cytoplasmically Synthesized Proteins into Membranous
... Locations of Protein Synthesis • ER Associated Ribosomes – proteins translocate co-translationally into ER lumen – subsequent processing & transport through Golgi for secretory vesicles and lysosomes ...
... Locations of Protein Synthesis • ER Associated Ribosomes – proteins translocate co-translationally into ER lumen – subsequent processing & transport through Golgi for secretory vesicles and lysosomes ...
simplified models for proteins in coarse
... force fields, since there is no native conformation to reproduce and they are prone to aggregation, so many proteins have to be included in the simulation. Existing transferable CG potentials work fine for IDP proteins, but not for natively folded proteins. 3- The higher the resolution, the better r ...
... force fields, since there is no native conformation to reproduce and they are prone to aggregation, so many proteins have to be included in the simulation. Existing transferable CG potentials work fine for IDP proteins, but not for natively folded proteins. 3- The higher the resolution, the better r ...
Media - Inside Cancer
... hence transmitting the message through the cell. 2. Since all cell signaling pathways end with a cellular response, explain why it is important that the cellular proteins that have been activated by the binding of the signal make their way to the nucleus. Hint: What role does the nucleus play in cel ...
... hence transmitting the message through the cell. 2. Since all cell signaling pathways end with a cellular response, explain why it is important that the cellular proteins that have been activated by the binding of the signal make their way to the nucleus. Hint: What role does the nucleus play in cel ...
Molecules of Life - Reading molecules of life
... antibodies, and muscle fiber. Enzymes are a type of protein that speed up chemical reactions. They are known as "biological catalysts." For example, your stomach would not be able to break down food if it did not have special enzymes to speed up the rate of digestion. Antibodies that protect you aga ...
... antibodies, and muscle fiber. Enzymes are a type of protein that speed up chemical reactions. They are known as "biological catalysts." For example, your stomach would not be able to break down food if it did not have special enzymes to speed up the rate of digestion. Antibodies that protect you aga ...
handout 1
... production of selected targets Specialized centers for technology development leading to high throughput structure determination of difficult proteins Specialized centers for protein structures relevant to disease (other NIH Institutes and Centers) Included in NIH Structural Biology Roadmap plan ...
... production of selected targets Specialized centers for technology development leading to high throughput structure determination of difficult proteins Specialized centers for protein structures relevant to disease (other NIH Institutes and Centers) Included in NIH Structural Biology Roadmap plan ...
RACK-1, a receptor for activated C kinase, links metabotropic
... Local protein synthesis is activated by glutamate in synaptoneurosomes (Weiler, Greenough PNAS, 90:7168, 1993). To search for transmitter receptor triggered mechanisms involved in translational control of dendritically localized mRNAs, we focussed on mRNPs that might be affected by second messenger ...
... Local protein synthesis is activated by glutamate in synaptoneurosomes (Weiler, Greenough PNAS, 90:7168, 1993). To search for transmitter receptor triggered mechanisms involved in translational control of dendritically localized mRNAs, we focussed on mRNPs that might be affected by second messenger ...
Supplementary Figures
... boiled in SDS-PAGE sample buffer for 3 minutes and sonicated to reduce viscosity of released phage DNA. Samples were electrophoresed through 10% acrylamide gels and protein bands visualized by Coomassie staining. Individual bands were excised and trypsinized. The peptide mixture of these digested pr ...
... boiled in SDS-PAGE sample buffer for 3 minutes and sonicated to reduce viscosity of released phage DNA. Samples were electrophoresed through 10% acrylamide gels and protein bands visualized by Coomassie staining. Individual bands were excised and trypsinized. The peptide mixture of these digested pr ...
Intracellular Compartments and Protein Sorting
... Signal sequence often at C-terminus Some proteins with sequence near N-terminus Peroxins (receptors, docking proteins) participate in transport Inherited defects in peroxin genes such as Zellweger syndrome ...
... Signal sequence often at C-terminus Some proteins with sequence near N-terminus Peroxins (receptors, docking proteins) participate in transport Inherited defects in peroxin genes such as Zellweger syndrome ...
Pfam
... • A protein family is a group of evolutionarilyrelated proteins • Proteins in a family descend from a common ancestor (homology) and typically have similar three-dimensional structures, functions, and significant sequence similarity. While it is difficult to evaluate the significance of functional o ...
... • A protein family is a group of evolutionarilyrelated proteins • Proteins in a family descend from a common ancestor (homology) and typically have similar three-dimensional structures, functions, and significant sequence similarity. While it is difficult to evaluate the significance of functional o ...
Steps in a Western blot
... secondary antibody, such as labeling an antibody-binding protein like Staphylococcus Protein A with a radioactive isotope of iodine. Since other methods are safer, quicker and cheaper this method is now rarely used. One step Historically, the probing process was performed in two steps because of the ...
... secondary antibody, such as labeling an antibody-binding protein like Staphylococcus Protein A with a radioactive isotope of iodine. Since other methods are safer, quicker and cheaper this method is now rarely used. One step Historically, the probing process was performed in two steps because of the ...
Support vector machines for protein function prediction
... • A protein is classified as either belong (+) or not belong (-) to a functional family • By screening against all families, the function of this protein can be identified (example: SVMProt) ...
... • A protein is classified as either belong (+) or not belong (-) to a functional family • By screening against all families, the function of this protein can be identified (example: SVMProt) ...
Bioinformatic Software in Web
... Analyzing protein phenotypes and protein post-translational modifications in fluid, cells, or tissues. Examining the clonality of immunoglobulins and detecting clones which are not seen with conventional techniques. Monitoring disease processes and protein expression. Discovering new disease markers ...
... Analyzing protein phenotypes and protein post-translational modifications in fluid, cells, or tissues. Examining the clonality of immunoglobulins and detecting clones which are not seen with conventional techniques. Monitoring disease processes and protein expression. Discovering new disease markers ...
A Brief Summary of Protein Targeting in Eukaryotes Or a brief
... help of chaperones and several membrane-bound proteins. The transport process also requires energy. For proteins with a signal peptide: Many proteins, including almost all secreted, ER and Golgi proteins, have N-terminal signal peptides which direct their translocation to the ER lumen during their ...
... help of chaperones and several membrane-bound proteins. The transport process also requires energy. For proteins with a signal peptide: Many proteins, including almost all secreted, ER and Golgi proteins, have N-terminal signal peptides which direct their translocation to the ER lumen during their ...
Introduction
... Turnover of cellular proteins was discovered in the 1930s in studies of Rudolf Schoenheimer, but it was in the 1960s that is became apparent that this was not just turnover, but a highly selective process. By the end of the 1970s two independent groups were working on two different topics: in the la ...
... Turnover of cellular proteins was discovered in the 1930s in studies of Rudolf Schoenheimer, but it was in the 1960s that is became apparent that this was not just turnover, but a highly selective process. By the end of the 1970s two independent groups were working on two different topics: in the la ...
Protein Targeting Notes
... help of chaperones and several membrane-bound proteins. The transport process also requires energy. For proteins with a signal peptide: Many proteins, including almost all secreted, ER and Golgi proteins, have N-terminal signal peptides which direct their translocation to the ER lumen during their ...
... help of chaperones and several membrane-bound proteins. The transport process also requires energy. For proteins with a signal peptide: Many proteins, including almost all secreted, ER and Golgi proteins, have N-terminal signal peptides which direct their translocation to the ER lumen during their ...
No Slide Title
... General Remarks Antibodies are a powerful tool for life science research They find multiple application in a variety of areas including biotechnology, medicine and diagnosis. Antibodies can recognize either linear or 3D epitopes There are rules to predict what peptide fragments from a protein are li ...
... General Remarks Antibodies are a powerful tool for life science research They find multiple application in a variety of areas including biotechnology, medicine and diagnosis. Antibodies can recognize either linear or 3D epitopes There are rules to predict what peptide fragments from a protein are li ...
ANIONIC EXCHANGE FRACTIONATION
... bioprocessor (Ciphergen Biosystems). After 60 min incubation at room temperature on a platform shaker, the arrays were washed three times (5 min) in 150 µL of binding buffer before being quickly rinsed twice with 100 µL of distilled H2O. The arrays were then removed from the bioprocessor system and ...
... bioprocessor (Ciphergen Biosystems). After 60 min incubation at room temperature on a platform shaker, the arrays were washed three times (5 min) in 150 µL of binding buffer before being quickly rinsed twice with 100 µL of distilled H2O. The arrays were then removed from the bioprocessor system and ...
No Slide Title
... Chemical modifications and processing alter the biological activity of proteins ...
... Chemical modifications and processing alter the biological activity of proteins ...
Proteomics

Proteomics is the large-scale study of proteins, particularly their structures and functions. Proteins are vital parts of living organisms, as they are the main components of the physiological metabolic pathways of cells. The term proteomics was first coined in 1997 to make an analogy with genomics, the study of the genome. The word proteome is a portmanteau of protein and genome, and was coined by Marc Wilkins in 1994 while working on the concept as a PhD student.The proteome is the entire set of proteins, produced or modified by an organism or system. This varies with time and distinct requirements, or stresses, that a cell or organism undergoes. Proteomics is an interdisciplinary domain formed on the basis of the research and development of the Human Genome Project; it is also emerging scientific research and exploration of proteomes from the overall level of intracellular protein composition, structure, and its own unique activity patterns. It is an important component of functional genomics.While proteomics generally refers to the large-scale experimental analysis of proteins, it is often specifically used for protein purification and mass spectrometry.