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Journal of Bacteriology
Journal of Bacteriology

... The nitrogenase of the free-living, microaerobic, N2-fixing bacterium AzospiriUum amazonense (strain Y1) was purified by chromatography on DEAE-52 cellulose, by heat treatment, and by preparative polyacrylamide gel electrophoresis. The specific nitrogenase activities were 2,400 nmol of C21[4 formed ...
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... The separated proteins are made visible by staining. 4) Density of each band is directly proprtional to its serum concentration, so albumin will show the most dense band ( serum albumin is 3.5-5.5 gm%, while globulins 1.8-3.3 gm%).  In case of decreased serum albumin (hypoalbuminuria), the albumin ...
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PowerPoint Presentation - Nerve activates contraction

... Consists of amino acids connected by peptide bonds Unique properties of each protein are determined by Type of amino acid Sequence of amino acid ...
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An ideal protein provider - Research

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... Anfinsen’s (1973) thermodynamic hypothesis: Proteins are not assembled into their native structures by a biological process, but folding is a purely physical process that depends only on the specific amino acid sequence of the protein. Anfinsen’s hypothesis implies that in principle protein structur ...
Basics of Fluorescence
Basics of Fluorescence

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Metabolism of Amino Acids
Metabolism of Amino Acids

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PDF - World Wide Journals

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Protein synthesis – the difference between prokaryotes and

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THE PROTEIN NON-FOLDING PROBLEM: AMINO ACID
THE PROTEIN NON-FOLDING PROBLEM: AMINO ACID

... disordered parts show significantly less sequence similarity than do the ordered parts (work in progress), suggesting that identification of disordered regions by homology is apparently an effective way to increase the information content after all. An additional problem is that a corresponding regi ...
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... Discrete protein sequence motifs are widely used to describe homology between proteins and establish relationships between well-known and new protein sequences. More specifically, discrete motifs identify amino acids sharing important properties conserved in evolution. Further, they are often able t ...
Comparative proteomics reveal characteristics of life
Comparative proteomics reveal characteristics of life

... [21]). We address this important life-history transition by using an electrospray tandem mass spectrometry approach for relative quantification of the whole-body proteome of nest workers and foragers. A common argument against whole-body analyses is that the contributions of different organs or tiss ...
Protein thermal stability: insights from atomic displacement
Protein thermal stability: insights from atomic displacement

... importance for industries where biocatalysts are used in extreme conditions to achieve higher solubility of substrates. It is, therefore, of primary importance to understand the factors that contribute to thermal stability. Knowledge regarding these factors has been accumulated from both experimenta ...
Proteomic analyses of the time course responses of mice infected
Proteomic analyses of the time course responses of mice infected

... Ragan et al., 2013), confirming that all animals were seronegative for brucellosis. In two infection groups, each animal was infected with 2 9 104 CFUs of either B. abortus or Y. enterocolitica through an intraperitoneal route. The remaining five mice were injected with sterile PBS as noninfected co ...
Unit 1 revision - Groby Bio Page
Unit 1 revision - Groby Bio Page

... from a region of lower concentration to a region of higher concentration using energy and carrier molecules • Explain how the carrier molecules help a molecule to enter the cell • The molecule binds to a receptor on the carrier protein. Inside the cell ATP binds to the protein and splits into ADP + ...
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What is Life? Project PART 6: The molecules of life

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Enzyme - My CCSD
Enzyme - My CCSD

... amino acids. These building blocks in various combinations make thousands of different kinds of proteins. Amino acids are linked together to form a protein by a bond called a peptide bond. A chain of amino acids bonded together is often called a polypeptide. Most proteins are made up of multiple pol ...
Xanthomonas campestris
Xanthomonas campestris

... when analyzing Xcc recovered from the resistant plant (REU) by 2DE. It seems that the presence of more plant proteins in REU is indeed a response of the resistant plant and not a technical artifact, especially since these results were consistent in all biological replicates by using two proteomic te ...
Translation: DNA to mRNA to Protein
Translation: DNA to mRNA to Protein

... The genes in DNA encode protein molecules, which are the "workhorses" of the cell, carrying out all the functions necessary for life. For example, enzymes, including those that metabolize nutrients and synthesize new cellular constituents, as well as DNA polymerases and other enzymes that make copie ...
7.06 Problem Set #5, Spring 2005
7.06 Problem Set #5, Spring 2005

... Your protein would only be able to be translocated when it is being translated at the same time. Therefore, the first experiment would result in translocation, but the second experiment would not. Proteins in mammalian cells that are destined for the secretory pathway are always co-translationally t ...
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Protein–protein interaction



Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.
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