The Nterminal region of the bacterial DNA polymerase PolC features
... removal of the second half of the PolC N-terminus would help to improve the results further. Therefore, we took only the fragment of the multiple sequence alignment covering the first half of the PolC N-terminus (corresponding to residues 1–89 of BsuPolC; residue numbering is based on BsuPolC through ...
... removal of the second half of the PolC N-terminus would help to improve the results further. Therefore, we took only the fragment of the multiple sequence alignment covering the first half of the PolC N-terminus (corresponding to residues 1–89 of BsuPolC; residue numbering is based on BsuPolC through ...
Chap. 6B Enzymes Introduction to Enzymes How Enzymes Work
... It is estimated that one-third of all proteins in a eukaryotic cell are phosphorylated, and one, or often many phosphorylation events are part of virtually every regulatory process. Some proteins have only one phosphorylation site, whereas others have several, and a few have dozens of sites for phos ...
... It is estimated that one-third of all proteins in a eukaryotic cell are phosphorylated, and one, or often many phosphorylation events are part of virtually every regulatory process. Some proteins have only one phosphorylation site, whereas others have several, and a few have dozens of sites for phos ...
REGISTRATION OF RUMINANT FEEDS - AFMA
... not normally consumed as feed by itself when in combination with other substances, whether or not it has a direct or indirect nutritional value and is not classified as a medicine or an essential nutrient, which affects the characteristics of feedstuffs, feed, food, animal products, animal productio ...
... not normally consumed as feed by itself when in combination with other substances, whether or not it has a direct or indirect nutritional value and is not classified as a medicine or an essential nutrient, which affects the characteristics of feedstuffs, feed, food, animal products, animal productio ...
Effects of Anions on the Positive Ion Electrospray Ionization Mass
... Model 2400-00 1) and electrosprayed in ambient laboratory air. The resulting highly charged droplets and solvated ions were transported into the vacuum of a quadrupole mass spectrometer (Vestec Model 201) through a 20-cm-long, 0.5mm4.d. heated capillary tube. The flow rate through the spray needle w ...
... Model 2400-00 1) and electrosprayed in ambient laboratory air. The resulting highly charged droplets and solvated ions were transported into the vacuum of a quadrupole mass spectrometer (Vestec Model 201) through a 20-cm-long, 0.5mm4.d. heated capillary tube. The flow rate through the spray needle w ...
Exact and Effective Pair-Wise Potential for Protein-Ligand Interactions Obtained from a Semiempirical Energy Partition
... spatial components. As this formalism was developed at a semiempirical quantum level, it enables also the complete separability of these components. Here, the SEEB formalism was extended to describe protein-ligand interactions using a pair-wise potential. The SEEB method was then used to study the a ...
... spatial components. As this formalism was developed at a semiempirical quantum level, it enables also the complete separability of these components. Here, the SEEB formalism was extended to describe protein-ligand interactions using a pair-wise potential. The SEEB method was then used to study the a ...
Nuclear hormone receptors (contd)
... – two basic regions are required for DNA binding – two groups of bHLH proteins exist • Class A are ubiquitously expressed (eg E12/E47) • Class B are tissue-specific (MyoD, myogenin) – a common strategy among tissue-specific proteins is to heterodimerize with ubiquitous partners – homodimers are not ...
... – two basic regions are required for DNA binding – two groups of bHLH proteins exist • Class A are ubiquitously expressed (eg E12/E47) • Class B are tissue-specific (MyoD, myogenin) – a common strategy among tissue-specific proteins is to heterodimerize with ubiquitous partners – homodimers are not ...
Anti-c-myc antibody 9E10 - Protein Engineering, Design and Selection
... the key amino acids remain the same as in the wild-type myctag. Lysine at position 3, glutamic acid at position 7 and leucine at position 10 (numbering according to the initial 11-mer peptide) appear to become more specific, since fewer substitutions are tolerated. However, this is probably due to t ...
... the key amino acids remain the same as in the wild-type myctag. Lysine at position 3, glutamic acid at position 7 and leucine at position 10 (numbering according to the initial 11-mer peptide) appear to become more specific, since fewer substitutions are tolerated. However, this is probably due to t ...
Protein synthesis: Twenty three amino acids and
... a particular amino acid, and so can be extended to other synthetase–tRNA pairs on a case-by-case basis for the selection (from combinatorial libraries) of many structurally diverse amino-acid analogs. It is an interesting coincidence that tyrosyl-tRNA synthetase, the crucial synthetase used in this ...
... a particular amino acid, and so can be extended to other synthetase–tRNA pairs on a case-by-case basis for the selection (from combinatorial libraries) of many structurally diverse amino-acid analogs. It is an interesting coincidence that tyrosyl-tRNA synthetase, the crucial synthetase used in this ...
Functional Analysis of Subunit e of the F1Fo
... the Fo-sector has been best characterized in rat mitochondrion studies, from which a 2:1 ratio of Su e subunits to F1Fo-ATP synthase complex was reported (2). A single hydrophobic segment located at the extreme N-terminal region of the Su e protein (approximately residues 4 to 21) is thought to anch ...
... the Fo-sector has been best characterized in rat mitochondrion studies, from which a 2:1 ratio of Su e subunits to F1Fo-ATP synthase complex was reported (2). A single hydrophobic segment located at the extreme N-terminal region of the Su e protein (approximately residues 4 to 21) is thought to anch ...
Duchenne muscular dystrophy:
... We hypothesized that DMD would be associated with significant changes in both energy and protein metabolism. We studied the resting energy expenditure (REE) in DMD and control children using indirect calorimetry, and their protein metabolism using an intravenous infusion of leucine and glutamine lab ...
... We hypothesized that DMD would be associated with significant changes in both energy and protein metabolism. We studied the resting energy expenditure (REE) in DMD and control children using indirect calorimetry, and their protein metabolism using an intravenous infusion of leucine and glutamine lab ...
G a - Pontificia Universidad Javeriana, Cali
... Using a Timed Concurrent Constraint Process Calculus for Modeling Biomolecular Interactions ...
... Using a Timed Concurrent Constraint Process Calculus for Modeling Biomolecular Interactions ...
MICROSTRUCTURE OF PROTEIN BODIES IN MARAMA BEAN SPECIES
... slightly different from the initial TEM (Fig. 3). This is possibly due to some components being leached out from the parenchyma cells during the incubation of sample in Proteinase K buffer. Proteinase K is a stable serine protease that digests native protein effectively (Ebeling, Hennrich, Klockow, ...
... slightly different from the initial TEM (Fig. 3). This is possibly due to some components being leached out from the parenchyma cells during the incubation of sample in Proteinase K buffer. Proteinase K is a stable serine protease that digests native protein effectively (Ebeling, Hennrich, Klockow, ...
Regulation of transcript encoding the 43K
... activity. Denervation causes a 10- to 100-fold increase in AChR mRNA and AChR protein (for review, see Anderson, 1987). These nonsynaptic AChRs are not clustered at high density, but rather are present at 20to 100-fold lower concentration than at the synapse. Although fluorescence and histochemical ...
... activity. Denervation causes a 10- to 100-fold increase in AChR mRNA and AChR protein (for review, see Anderson, 1987). These nonsynaptic AChRs are not clustered at high density, but rather are present at 20to 100-fold lower concentration than at the synapse. Although fluorescence and histochemical ...
Targeted Identification of Glycoproteins in Disease
... present on bacterial cell surfaces provide one such target.(2) Importantly, glycosylation patterns vary between bacterial and mammalian species, as well as among different bacterial species. (3) This variation creates the potential to selectively target a bacterial species based on cell-surface glyc ...
... present on bacterial cell surfaces provide one such target.(2) Importantly, glycosylation patterns vary between bacterial and mammalian species, as well as among different bacterial species. (3) This variation creates the potential to selectively target a bacterial species based on cell-surface glyc ...
3. The Molecules of Cells
... of biological molecules to the functioning of living cells and to human health • Molecular interactions, such as those between the gene for lactase production, the enzyme lactase, and the milk sugar lactose, drive all biological processes ...
... of biological molecules to the functioning of living cells and to human health • Molecular interactions, such as those between the gene for lactase production, the enzyme lactase, and the milk sugar lactose, drive all biological processes ...
Beta Structures
... Retinol is bound inside the barrel, between the two b sheets, such that its only hydrophilic part (an OH tail) is at the surface of the molecule. Exhibits only minimal conformational changes in both apo- (open) and holo- (bound) forms. ...
... Retinol is bound inside the barrel, between the two b sheets, such that its only hydrophilic part (an OH tail) is at the surface of the molecule. Exhibits only minimal conformational changes in both apo- (open) and holo- (bound) forms. ...
Article
... Beans were called "poor man's meat" because until the mid-twentieth century, when meat was still a scarce and precious commodity, legumes were the main source of protein, especially for people who, living far from the sea, could not take advantage of fishing. The proteins of legumes are not as compl ...
... Beans were called "poor man's meat" because until the mid-twentieth century, when meat was still a scarce and precious commodity, legumes were the main source of protein, especially for people who, living far from the sea, could not take advantage of fishing. The proteins of legumes are not as compl ...
Chemical Nature of the Amino Acids
... All peptides and polypeptides are polymers of alphaamino acids. There are 20 a-amino acids that are relevant to the make-up of mammalian proteins (see below). Several other amino acids are found in the body free or in combined states (i.e. not associated with peptides or proteins). These non-protein ...
... All peptides and polypeptides are polymers of alphaamino acids. There are 20 a-amino acids that are relevant to the make-up of mammalian proteins (see below). Several other amino acids are found in the body free or in combined states (i.e. not associated with peptides or proteins). These non-protein ...
invited talk
... RF1 (UAG, UAA) and RF2 (UGA, UAA) Fragment of the alignment (117 pairs). SDPs are shown by black boxes above the alignment. ...
... RF1 (UAG, UAA) and RF2 (UGA, UAA) Fragment of the alignment (117 pairs). SDPs are shown by black boxes above the alignment. ...
PDF Datastream - Brown Digital Repository
... have been steadily increasing due to the promise of expanding our understanding of fundamental bacterial processes such as cell wall biogenesis as well as the promise of finding potential vaccine- or drug-targets for virulent bacteria. Meanwhile, rapid development in mass spectrometry based proteomi ...
... have been steadily increasing due to the promise of expanding our understanding of fundamental bacterial processes such as cell wall biogenesis as well as the promise of finding potential vaccine- or drug-targets for virulent bacteria. Meanwhile, rapid development in mass spectrometry based proteomi ...
The TEA Transcription Factor Tec1 Links TOR and MAPK Pathways
... current view that the TORC1 and Ras/PKA pathways act in parallel in the control of cell division (Rohde et al. 2008). With respect to controlling cellular development, the Ras/ PKA pathway is known to regulate adhesive and filamentous growth via the catalytic subunit Tpk2, which targets the transcrip ...
... current view that the TORC1 and Ras/PKA pathways act in parallel in the control of cell division (Rohde et al. 2008). With respect to controlling cellular development, the Ras/ PKA pathway is known to regulate adhesive and filamentous growth via the catalytic subunit Tpk2, which targets the transcrip ...
2.3. Three-Dimensional structure and function of proteins.
... - Some of them show specific biological roles, but in other cases they are just part of the main structural and functional peptide. ...
... - Some of them show specific biological roles, but in other cases they are just part of the main structural and functional peptide. ...
Nutrition for Endurance Athletes
... should supply the body with high grade protein to support recovery. Because exercise puts a strain on the muscles and this can trigger catabolic processes (muscle loss). In order to prevent this, it is extremely important to fuel the body with protein. Regeneration is accelerated, muscles recover mo ...
... should supply the body with high grade protein to support recovery. Because exercise puts a strain on the muscles and this can trigger catabolic processes (muscle loss). In order to prevent this, it is extremely important to fuel the body with protein. Regeneration is accelerated, muscles recover mo ...
application of recombinant smr-domain containing protein of
... Abstract. The aim of this study was to find novel proteins expressed from an Angiostrongylus cantonensis adult female worm cDNA library for serodiagnosis of angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with pooled positive sera from proven angiostrongyliasis ...
... Abstract. The aim of this study was to find novel proteins expressed from an Angiostrongylus cantonensis adult female worm cDNA library for serodiagnosis of angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with pooled positive sera from proven angiostrongyliasis ...
9.1 Amino Acids—A Second Look, Continued
... Proteins are classified into groups based on their three-dimensional shape. • Globular proteins are compact, spherical structures that are soluble in an aqueous environment. Myoglobin, which stores oxygen in muscle, is an example. • Fibrous proteins are long, threadlike structures that have high hel ...
... Proteins are classified into groups based on their three-dimensional shape. • Globular proteins are compact, spherical structures that are soluble in an aqueous environment. Myoglobin, which stores oxygen in muscle, is an example. • Fibrous proteins are long, threadlike structures that have high hel ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.