Direct visualization of protein interactions in plant cells
... complementary DNAs NPR1/NIM1 and TGA2 from Arabidopsis were amplified by PCR with Not1 and Cla1 linkers at the 5′ and 3′ ends of the genes, respectively. NPR1-1 was created from the NPR1 clone by using the ExSite PCR-based site-directed mutagenesis kit (Strategene, La Jolla, CA). The complementary m ...
... complementary DNAs NPR1/NIM1 and TGA2 from Arabidopsis were amplified by PCR with Not1 and Cla1 linkers at the 5′ and 3′ ends of the genes, respectively. NPR1-1 was created from the NPR1 clone by using the ExSite PCR-based site-directed mutagenesis kit (Strategene, La Jolla, CA). The complementary m ...
Adaptation of Sucrose Metabolism in the Escherichia coli Wild
... Finally, we isolated and characterized mutants with chromosomal and plasmid-borne mutations that grow faster on sucrose than the wild type. Analysis of such adaptational mutations provided evidence that the basal expression level of cscB or the transport activity of the sucrose permease in the wildt ...
... Finally, we isolated and characterized mutants with chromosomal and plasmid-borne mutations that grow faster on sucrose than the wild type. Analysis of such adaptational mutations provided evidence that the basal expression level of cscB or the transport activity of the sucrose permease in the wildt ...
Study of the Effects of High-Energy Proton Beams
... Without proton irradiation, bacterial cells were monitored for 57 h by measuring the culture turbidity spectrophotometrically to obtain OD600 values, optical density at 600 nm. After irradiation, 0.1 mL of the radiation-exposed bacteria solution were reseeded in 5 mL of fresh LB medium and allowed t ...
... Without proton irradiation, bacterial cells were monitored for 57 h by measuring the culture turbidity spectrophotometrically to obtain OD600 values, optical density at 600 nm. After irradiation, 0.1 mL of the radiation-exposed bacteria solution were reseeded in 5 mL of fresh LB medium and allowed t ...
Specialized adaptation of a lactic acid bacterium to the milk
... elements as a significant contributor to the genome diversity in S. thermophilus. Deduced proteins from 57 (45%) of the 127 LMD-9 specific ORFs have no orthologous counterpart in other streptococcal species (see below), indicating they likely originated via lateral gene transfer (LGT). Approximately ...
... elements as a significant contributor to the genome diversity in S. thermophilus. Deduced proteins from 57 (45%) of the 127 LMD-9 specific ORFs have no orthologous counterpart in other streptococcal species (see below), indicating they likely originated via lateral gene transfer (LGT). Approximately ...
“Genes” and “Mutations” - Native American Cancer Research
... The injury is a mutation resulting in damage that is passed on from the first body (somatic) cell as it divides into additional cells It gives the cells harboring the mutation an advantage to outgrow other cells For example in lung cancer the carcinogens in cigarette smoke may cause damage in severa ...
... The injury is a mutation resulting in damage that is passed on from the first body (somatic) cell as it divides into additional cells It gives the cells harboring the mutation an advantage to outgrow other cells For example in lung cancer the carcinogens in cigarette smoke may cause damage in severa ...
Statistical analysis of DNA microarray data
... GEO DataSets (GDS) are curated sets of GEO Sample data. A GDS record represents a collection of biologically and statistically comparable GEO Samples and forms the basis of GEO's suite of data display and analysis tools. Samples within a GDS refer to the same Platform, that is, they share a common ...
... GEO DataSets (GDS) are curated sets of GEO Sample data. A GDS record represents a collection of biologically and statistically comparable GEO Samples and forms the basis of GEO's suite of data display and analysis tools. Samples within a GDS refer to the same Platform, that is, they share a common ...
Intracellular killing of bacteria: is Dictyostelium a model macrophage
... Kypri et al., 2007). As seen for wash KO cells, lvsB KO cells also have specific growth defects: they are unable to feed upon M. luteus and some pathogenic Klebsiella strains, but can grow as efficiently as WT cells on other bacteria, as Bacillus and E. coli. Overall, the general conclusion of these ...
... Kypri et al., 2007). As seen for wash KO cells, lvsB KO cells also have specific growth defects: they are unable to feed upon M. luteus and some pathogenic Klebsiella strains, but can grow as efficiently as WT cells on other bacteria, as Bacillus and E. coli. Overall, the general conclusion of these ...
Linköping University Post Print Neuronal Subtype Specification within a Feed-Forward Loops
... It is becomingly increasingly clear that progenitor cells, both in the invertebrate and vertebrate CNS, undergo critical temporal transitions resulting in changes in their competence (reviewed in (Jacob et al., 2008; Okano and Temple, 2009). This is evident by the stereotyped appearance of different ...
... It is becomingly increasingly clear that progenitor cells, both in the invertebrate and vertebrate CNS, undergo critical temporal transitions resulting in changes in their competence (reviewed in (Jacob et al., 2008; Okano and Temple, 2009). This is evident by the stereotyped appearance of different ...
Introduction To Genetics- Chapter 11
... passed on from one generation to another. Mendel knew nothing about chromosomes, genes, or DNA. Why? These terms hadn’t yet been defined. ...
... passed on from one generation to another. Mendel knew nothing about chromosomes, genes, or DNA. Why? These terms hadn’t yet been defined. ...
Author`s personal copy
... 0.17–0.18 mm, B. Braun Biotech International GmbH, Melsungen, Germany). Cell lysis was undertaken with a bead beater (FastPrep® -24, MP Biomedicals, Heidelberg, Germany) for 45 s with 6.5 m s−1 , and samples were centrifuged to remove cell debris (16,000 × g, 4 ◦ C, 5 min). DNA in the supernatant wa ...
... 0.17–0.18 mm, B. Braun Biotech International GmbH, Melsungen, Germany). Cell lysis was undertaken with a bead beater (FastPrep® -24, MP Biomedicals, Heidelberg, Germany) for 45 s with 6.5 m s−1 , and samples were centrifuged to remove cell debris (16,000 × g, 4 ◦ C, 5 min). DNA in the supernatant wa ...
Genetically Essential and Nonessential a-Tubulin Genes Specify Functionally Interchangeable Proteins.
... P-tubulin has been made previously in many species (for reviews, see references 10 and 29). For example, the fission yeast Schizosaccharomyces pombe also has a single ,Btubulin gene and two a-tubulin genes (13, 50). The frequent occurrence of such families of closely related genes leads to questions ...
... P-tubulin has been made previously in many species (for reviews, see references 10 and 29). For example, the fission yeast Schizosaccharomyces pombe also has a single ,Btubulin gene and two a-tubulin genes (13, 50). The frequent occurrence of such families of closely related genes leads to questions ...
Antioxidant and Xenobiotic-metabolizing Enzyme Gene Expression
... detoxifying enzymes was decreased 2- to 4-fold in the doxorubicinresistant cells. Southern blot analysis of l'xtÃ--and Afs/7l-reslricled gen- the gene coding for selenium-dependent glutathione peroxidase, omic DNA revealed no evidence for amplification or rearrangement of as well as the other antiox ...
... detoxifying enzymes was decreased 2- to 4-fold in the doxorubicinresistant cells. Southern blot analysis of l'xtÃ--and Afs/7l-reslricled gen- the gene coding for selenium-dependent glutathione peroxidase, omic DNA revealed no evidence for amplification or rearrangement of as well as the other antiox ...
video slide
... X Inactivation in Female Mammals • In mammalian females, one of the two X chromosomes in each cell is randomly inactivated during embryonic development • The inactive X condenses into a Barr body • If a female is heterozygous for a particular gene located on the X chromosome, she will be a mosaic f ...
... X Inactivation in Female Mammals • In mammalian females, one of the two X chromosomes in each cell is randomly inactivated during embryonic development • The inactive X condenses into a Barr body • If a female is heterozygous for a particular gene located on the X chromosome, she will be a mosaic f ...
Leukaemia Section t(2;3)(p21;q26) THADA/MECOM Atlas of Genetics and Cytogenetics in Oncology and Haematology
... zinc finger motifs, respectively, a repression domain between the two sets of zinc fingers, and an acidic domain at its C-term. ...
... zinc finger motifs, respectively, a repression domain between the two sets of zinc fingers, and an acidic domain at its C-term. ...
2006a Tests of parallel molecular evolution in a long
... nonsynonymous point mutation in both pykF (Fig. 1 A) and nadR (Fig. 1B), with the exception of the focal populations that contained the previously discovered insertion mutations that led to identification of these candidate genes. One synonymous mutation was found in pykF, and none in nadR. The two ...
... nonsynonymous point mutation in both pykF (Fig. 1 A) and nadR (Fig. 1B), with the exception of the focal populations that contained the previously discovered insertion mutations that led to identification of these candidate genes. One synonymous mutation was found in pykF, and none in nadR. The two ...
DNA methylation profiling identifies epigenetic dysregulation in
... methylation status of 27 578 CpG sites corresponding to 14 475 consensus coding sequences and well-known cancer genes (Bibikova et al, 2009). Although the Infinium methylation assay is not a genome-wide DNA methylation technology, it is a useful screening tool that is sensitive, specific and highly re ...
... methylation status of 27 578 CpG sites corresponding to 14 475 consensus coding sequences and well-known cancer genes (Bibikova et al, 2009). Although the Infinium methylation assay is not a genome-wide DNA methylation technology, it is a useful screening tool that is sensitive, specific and highly re ...
Methods of Human Heredity Study
... centromeres are constant, and are called constitutive heterochromatic regions serving as chromosome markers. There are other heterochromatic regions, called facultative heterochromatin and represented by whole sex chromosomes, which become heterochromatic only at certain stages. For instance, in fem ...
... centromeres are constant, and are called constitutive heterochromatic regions serving as chromosome markers. There are other heterochromatic regions, called facultative heterochromatin and represented by whole sex chromosomes, which become heterochromatic only at certain stages. For instance, in fem ...
Genetic Approaches to Studying Genome Function
... mutation. Examples: A nonsense mutation in a gene can be suppressed by a mutation in a tRNA gene in which the anticodon has been mutated to read the nonsense codon (informational suppressor). A temperature-sensitive mutation that destabilizes a protein can be corrected by a compensating mutation in ...
... mutation. Examples: A nonsense mutation in a gene can be suppressed by a mutation in a tRNA gene in which the anticodon has been mutated to read the nonsense codon (informational suppressor). A temperature-sensitive mutation that destabilizes a protein can be corrected by a compensating mutation in ...
The complement C3 protein family in invertebrates
... The classical pathway, termed so because it was discovered first among three pathways, is initiated by binding of C1 to antigen-bound antibody, and results in the formation of the classical pathway C3 convertase composed of C4 and C2, which is responsible for the proteolytic activation of the centra ...
... The classical pathway, termed so because it was discovered first among three pathways, is initiated by binding of C1 to antigen-bound antibody, and results in the formation of the classical pathway C3 convertase composed of C4 and C2, which is responsible for the proteolytic activation of the centra ...
MayerFrankiPoster
... assembled it was introduced to E. coli. Figure 5 shows E. coli colonies (transformants) that were selected for growth on medium containing chloramphenicol (Cm), which allows only the growth of cells that have taken up the ∆cpcB inactivation plasmid. Several of these colonies were inoculated into liq ...
... assembled it was introduced to E. coli. Figure 5 shows E. coli colonies (transformants) that were selected for growth on medium containing chloramphenicol (Cm), which allows only the growth of cells that have taken up the ∆cpcB inactivation plasmid. Several of these colonies were inoculated into liq ...
Review #3 - California Lutheran University
... mutations. How is it organized and how did it minimize problems? How does wobble work, and how is it involved in “loosening” the base pairing rules? Which experiments showed how the code works: how many bases per codon, whether there were gaps or punctuation, and whether the codons overlapped? What ...
... mutations. How is it organized and how did it minimize problems? How does wobble work, and how is it involved in “loosening” the base pairing rules? Which experiments showed how the code works: how many bases per codon, whether there were gaps or punctuation, and whether the codons overlapped? What ...